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  1. Article ; Online: Primary cilia as dynamic and diverse signalling hubs in development and disease.

    Mill, Pleasantine / Christensen, Søren T / Pedersen, Lotte B

    Nature reviews. Genetics

    2023  Volume 24, Issue 7, Page(s) 421–441

    Abstract: Primary cilia, antenna-like sensory organelles protruding from the surface of most vertebrate cell types, are essential for regulating signalling pathways during development and adult homeostasis. Mutations in genes affecting cilia cause an overlapping ... ...

    Abstract Primary cilia, antenna-like sensory organelles protruding from the surface of most vertebrate cell types, are essential for regulating signalling pathways during development and adult homeostasis. Mutations in genes affecting cilia cause an overlapping spectrum of >30 human diseases and syndromes, the ciliopathies. Given the immense structural and functional diversity of the mammalian cilia repertoire, there is a growing disconnect between patient genotype and associated phenotypes, with variable severity and expressivity characteristic of the ciliopathies as a group. Recent technological developments are rapidly advancing our understanding of the complex mechanisms that control biogenesis and function of primary cilia across a range of cell types and are starting to tackle this diversity. Here, we examine the structural and functional diversity of primary cilia, their dynamic regulation in different cellular and developmental contexts and their disruption in disease.
    MeSH term(s) Adult ; Animals ; Humans ; Cilia/genetics ; Cilia/metabolism ; Signal Transduction ; Ciliopathies/genetics ; Ciliopathies/metabolism ; Mammals
    Language English
    Publishing date 2023-04-18
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2035157-4
    ISSN 1471-0064 ; 1471-0056
    ISSN (online) 1471-0064
    ISSN 1471-0056
    DOI 10.1038/s41576-023-00587-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Tubulin transport in cilia: How many tubulin cargo-binding sites per IFT particle? (retrospective on DOI 10.1002/bies.201400007).

    Pedersen, Lotte B

    BioEssays : news and reviews in molecular, cellular and developmental biology

    2017  Volume 39, Issue 1, Page(s) 1

    Language English
    Publishing date 2017-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 50140-2
    ISSN 1521-1878 ; 0265-9247
    ISSN (online) 1521-1878
    ISSN 0265-9247
    DOI 10.1002/bies.201600201
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  3. Article: Editorial.

    Pedersen, Lotte B

    Cilia

    2014  Volume 3, Page(s) 8

    Language English
    Publishing date 2014-08-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2689513-4
    ISSN 2046-2530
    ISSN 2046-2530
    DOI 10.1186/2046-2530-3-8
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  4. Article ; Online: Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.

    Kuhns, Stefanie / Juhl, Alice Dupont / Anvarian, Zeinab / Wüstner, Daniel / Pedersen, Lotte B / Andersen, Jens S

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2725, Page(s) 147–166

    Abstract: CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in ... ...

    Abstract CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.
    MeSH term(s) Humans ; Protein Transport/genetics ; Cilia/metabolism ; Proteins/metabolism ; Biological Transport
    Chemical Substances Proteins
    Language English
    Publishing date 2023-10-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3507-0_9
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  5. Article: DLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2.

    Rezi, Csenge K / Aslanyan, Mariam G / Diwan, Gaurav D / Cheng, Tao / Chamlali, Mohamed / Junger, Katrin / Anvarian, Zeinab / Lorentzen, Esben / Pauly, Kleo B / Afshar-Bahadori, Yasmin / Fernandes, Eduardo F A / Qian, Feng / Tosi, Sébastien / Christensen, Søren T / Pedersen, Stine F / Strømgaard, Kristian / Russell, Robert B / Miner, Jeffrey H / Mahjoub, Moe R /
    Boldt, Karsten / Roepman, Ronald / Pedersen, Lotte B

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Polarized vesicular trafficking directs specific receptors and ion channels to cilia, but the underlying mechanisms are poorly understood. Here we describe a role for DLG1, a core component of the Scribble polarity complex, in regulating ciliary protein ... ...

    Abstract Polarized vesicular trafficking directs specific receptors and ion channels to cilia, but the underlying mechanisms are poorly understood. Here we describe a role for DLG1, a core component of the Scribble polarity complex, in regulating ciliary protein trafficking in kidney epithelial cells. Conditional knockout of
    Language English
    Publishing date 2024-03-14
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.11.10.566524
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: 3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines.

    Frikstad, Kari-Anne M / Schink, Kay O / Gilani, Sania / Pedersen, Lotte B / Patzke, Sebastian

    Bio-protocol

    2022  Volume 12, Issue 6, Page(s) e4360

    Abstract: The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the ... ...

    Abstract The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the centrosome drives its duplication, and enables its microtubule-organizing activity and capability to form the primary cilium, which extends from the mature (mother) centriole, as the cell exits the cell cycle. Centrosomes and primary cilia are essential to human health, signified by the causal role of centrosome- and cilia-aberrations in numerous congenic disorders, as well as in the etiology and progression of cancer. The list of disease-associated centrosomal proteins and their proximitomes is steadily expanding, emphasizing the need for high resolution mapping of such proteins to specific substructures of the organelle. Here, we provide a detailed 3D-structured illumination microscopy (3D-SIM) protocol for comparative localization analysis of fluorescently labeled proteins at the centrosome in fixed human cell lines, at approximately 120 nm lateral and 300 nm axial resolution. The procedure was optimized to work with primary antibodies previously known to depend on more disruptive fixation reagents, yet largely preserves centriole and centrosome architecture, as shown by transposing acquired images of landmark proteins on previously published transmission electron microscopy (TEM) images of centrosomes. Even more advantageously, it is compatible with fluorescent protein tags. Finally, we introduce an internal reference to ensure correct 3D channel alignment. This protocol hence enables flexible, swift, and information-rich localization and interdependence analyses of centrosomal proteins, as well as their disorder-associated mutations.
    Language English
    Publishing date 2022-03-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4360
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  7. Article: A targeted multi-proteomics approach generates a blueprint of the ciliary ubiquitinome.

    Aslanyan, Mariam G / Doornbos, Cenna / Diwan, Gaurav D / Anvarian, Zeinab / Beyer, Tina / Junger, Katrin / van Beersum, Sylvia E C / Russell, Robert B / Ueffing, Marius / Ludwig, Alexander / Boldt, Karsten / Pedersen, Lotte B / Roepman, Ronald

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1113656

    Abstract: Establishment and maintenance of the primary cilium as a signaling-competent organelle requires a high degree of fine tuning, which is at least in part achieved by a variety of post-translational modifications. One such modification is ubiquitination. ... ...

    Abstract Establishment and maintenance of the primary cilium as a signaling-competent organelle requires a high degree of fine tuning, which is at least in part achieved by a variety of post-translational modifications. One such modification is ubiquitination. The small and highly conserved ubiquitin protein possesses a unique versatility in regulating protein function
    Language English
    Publishing date 2023-01-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1113656
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  8. Article ; Online: Analysis of Caveolin in Primary Cilia.

    Morthorst, Stine K / Mogensen, Johanne B / Christensen, Søren T / Pedersen, Lotte B

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2169, Page(s) 27–41

    Abstract: Recent evidence has indicated that caveolins are localized at the base of primary cilia, which are microtubule-based sensory organelles present on the cell surface, and that Caveolin-1 (CAV1) plays important roles in regulating ciliary membrane ... ...

    Abstract Recent evidence has indicated that caveolins are localized at the base of primary cilia, which are microtubule-based sensory organelles present on the cell surface, and that Caveolin-1 (CAV1) plays important roles in regulating ciliary membrane composition and function. Here we describe methods to analyze the localization and function of CAV1 in primary cilia of cultured mammalian cells. These include methods for culturing and transfecting mammalian cells with a CAV1-encoding plasmid or small interfering RNA (siRNA), analysis of mammalian cells by immunofluorescence microscopy (IFM) with antibodies against ciliary markers and CAV1, as well as methods for analyzing ciliary CAV1 function in siRNA-treated cells by IFM and cell-based signaling assays.
    MeSH term(s) Animals ; Caveolin 1/genetics ; Caveolin 1/metabolism ; Cell Culture Techniques/methods ; Cell Line ; Cells, Cultured ; Cilia/metabolism ; Humans ; Microscopy, Fluorescence/methods ; RNA, Small Interfering ; Real-Time Polymerase Chain Reaction/methods ; Signal Transduction/genetics
    Chemical Substances Caveolin 1 ; RNA, Small Interfering
    Language English
    Publishing date 2020-06-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0732-9_3
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  9. Article ; Online: The effect of traditional diet on glucose homoeostasis in carriers and non-carriers of a common

    Lewis, Jack Ivor / Lind, Mads Vendelbo / Møller, Grith / Hansen, Torben / Pedersen, Hanne / Christensen, Marie Mathilde Bjerg / Laursen, Jens Christian / Nielsen, Sara / Ottendahl, Charlotte B / Larsen, Christina V Lytken / Stark, Ken D / Bjerregaard, Peter / Jørgensen, Marit E / Lauritzen, Lotte

    The British journal of nutrition

    2023  , Page(s) 1–14

    Abstract: Consumption of traditional foods is decreasing amid a lifestyle transition in Greenland as incidence of type 2 diabetes (T2D) increases. In homozygous carriers of ... ...

    Abstract Consumption of traditional foods is decreasing amid a lifestyle transition in Greenland as incidence of type 2 diabetes (T2D) increases. In homozygous carriers of a
    Language English
    Publishing date 2023-05-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 280396-3
    ISSN 1475-2662 ; 0007-1145
    ISSN (online) 1475-2662
    ISSN 0007-1145
    DOI 10.1017/S000711452300106X
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  10. Article: Reproductive and Endocrine Outcomes in a Cohort of Danish Women following Auto-Transplantation of Frozen/Thawed Ovarian Tissue from a Single Center.

    Colmorn, Lotte B / Pedersen, Anette T / Larsen, Elisabeth C / Hansen, Alexandra S / Rosendahl, Mikkel / Andersen, Claus Yding / Kristensen, Stine G / Macklon, Kirsten T

    Cancers

    2022  Volume 14, Issue 23

    Abstract: Ovarian tissue cryopreservation (OTC) is a method of fertility preservation in girls and young women prior to gonadotoxic treatment. It is a safe and promising method to restore fertility. The initial recovery of endocrine function is high, but the ... ...

    Abstract Ovarian tissue cryopreservation (OTC) is a method of fertility preservation in girls and young women prior to gonadotoxic treatment. It is a safe and promising method to restore fertility. The initial recovery of endocrine function is high, but the longevity of the grafted tissue varies. In this single-center, combined retro- and prospective cohort study, we report the reproductive outcome and hormonal recovery following ovarian tissue transplantation (OTT) and evaluate possible predictors of the chance of pregnancy. The study includes 40 women from eastern Denmark undergoing 53 OTTs between 2003 and 2021. Permission to obtain retrospective data was given by the Danish Patient Safety Authorities and prospective data-collection by informed consent. Initial recovery of endocrine function was seen in 18/19 women with POI, and ongoing function of the grafted tissue in 7/14 two years from OTT. Live birth rate (LBR) was 41%, with 20 children to 39 women trying to conceive. Women who conceived had higher AFC at the time of OTC than women who did not (
    Language English
    Publishing date 2022-11-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14235873
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