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Article ; Online: Multiplexed Ultrasensitive Sample-to-Answer RT-LAMP Chip for the Identification of SARS-CoV-2 and Influenza Viruses.

Song, Minsun / Hong, SoonGweon / Lee, Luke P

Advanced materials (Deerfield Beach, Fla.)

2023 Volume 35, Issue 10, Page(s) e2207138

Abstract: Prompt on-site diagnosis of SARS-CoV-2 with other respiratory infections will have minimized the global impact of the COVID-19 pandemic through rapid, effective management. However, no such multiplex point-of-care (POC) chip has satisfied a suitable ... ...

Abstract	Prompt on-site diagnosis of SARS-CoV-2 with other respiratory infections will have minimized the global impact of the COVID-19 pandemic through rapid, effective management. However, no such multiplex point-of-care (POC) chip has satisfied a suitable sensitivity of gold-standard nucleic acid amplification tests (NAATs). Here, a rapid multiplexed ultrasensitive sample-to-answer loop-mediated isothermal amplification (MUSAL) chip operated by simple LED-driven photothermal amplification to detect six targets from single-swab sampling is presented. First, the MUSAL chip allows ultrafast on-chip sample preparation with ≈500-fold preconcentration at a rate of 1.2 mL min
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19 ; Clinical Laboratory Techniques ; COVID-19 Testing ; Pandemics ; Sensitivity and Specificity ; Nucleic Acid Amplification Techniques ; Orthomyxoviridae
Language	English
Publishing date	2023-01-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1474949-X
ISSN	1521-4095 ; 0935-9648
ISSN (online)	1521-4095
ISSN	0935-9648
DOI	10.1002/adma.202207138
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Preclinical Animal Models for Dravet Syndrome: Seizure Phenotypes, Comorbidities and Drug Screening.

Griffin, Aliesha / Hamling, Kyla R / Hong, SoonGweon / Anvar, Mana / Lee, Luke P / Baraban, Scott C

Frontiers in pharmacology

2018 Volume 9, Page(s) 573

Abstract: Epilepsy is a common chronic neurological disease affecting almost 3 million people in the United States and 50 million people worldwide. Despite availability of more than two dozen FDA-approved anti-epileptic drugs (AEDs), one-third of patients fail to ... ...

Abstract	Epilepsy is a common chronic neurological disease affecting almost 3 million people in the United States and 50 million people worldwide. Despite availability of more than two dozen FDA-approved anti-epileptic drugs (AEDs), one-third of patients fail to receive adequate seizure control. Specifically, pediatric genetic epilepsies are often the most severe, debilitating and pharmaco-resistant forms of epilepsy. Epileptic syndromes share a common symptom of unprovoked seizures. While some epilepsies/forms of epilepsy are the result of acquired insults such as head trauma, febrile seizure, or viral infection, others have a genetic basis. The discovery of epilepsy associated genes suggests varied underlying pathologies and opens the door for development of new "personalized" treatment options for each genetic epilepsy. Among these, Dravet syndrome (DS) has received substantial attention for both the pre-clinical and early clinical development of novel therapeutics. Despite these advances, there is no FDA-approved treatment for DS. Over 80% of patients diagnosed with DS carry a
Language	English
Publishing date	2018-06-04
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2018.00573
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: KIM-1/TIM-1 is a Receptor for SARS-CoV-2 in Lung and Kidney.

Mori, Yutaro / Fink, Corby / Ichimura, Takaharu / Sako, Keisuke / Mori, Makiko / Lee, Nathan N / Aschauer, Philipp / Padmanabha Das, Krishna M / Hong, SoonGweon / Song, Minsun / Padera, Robert F / Weins, Astrid / Lee, Luke P / Nasr, Mahmoud L / Dekaban, Gregory A / Dikeakos, Jimmy D / Bonventre, Joseph V

medRxiv : the preprint server for health sciences

2022

Abstract: SARS-CoV-2 precipitates respiratory distress by infection of airway epithelial cells and is often accompanied by acute kidney injury. We report that Kidney Injury Molecule-1/T cell immunoglobulin mucin domain 1 (KIM-1/TIM-1) is expressed in lung and ... ...

Abstract	SARS-CoV-2 precipitates respiratory distress by infection of airway epithelial cells and is often accompanied by acute kidney injury. We report that Kidney Injury Molecule-1/T cell immunoglobulin mucin domain 1 (KIM-1/TIM-1) is expressed in lung and kidney epithelial cells in COVID-19 patients and is a receptor for SARS-CoV-2. Human and mouse lung and kidney epithelial cells express KIM-1 and endocytose nanoparticles displaying the SARS-CoV-2 spike protein (virosomes). Uptake was inhibited by anti-KIM-1 antibodies and TW-37, a newly discovered inhibitor of KIM-1-mediated endocytosis. Enhanced KIM-1 expression by human kidney tubuloids increased uptake of virosomes. KIM-1 binds to the SARS-CoV-2 Spike protein
Keywords	covid19
Language	English
Publishing date	2022-01-11
Publishing country	United States
Document type	Preprint
DOI	10.1101/2020.09.16.20190694
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Article ; Online: Asymmetric Cell Division of Fibroblasts is An Early Deterministic Step to Generate Elite Cells during Cell Reprogramming

Yang Song / Jennifer Soto / Pingping Wang / Qin An / Xuexiang Zhang / SoonGweon Hong / Luke P. Lee / Guoping Fan / Li Yang / Song Li

Advanced Science, Vol 8, Iss 7, Pp n/a-n/a (2021)

2021

Abstract: Abstract Cell reprogramming is considered a stochastic process, and it is not clear which cells are prone to be reprogrammed and whether a deterministic step exists. Here, asymmetric cell division (ACD) at the early stage of induced neuronal (iN) ... ...

Abstract	Abstract Cell reprogramming is considered a stochastic process, and it is not clear which cells are prone to be reprogrammed and whether a deterministic step exists. Here, asymmetric cell division (ACD) at the early stage of induced neuronal (iN) reprogramming is shown to play a deterministic role in generating elite cells for reprogramming. Within one day, fibroblasts underwent ACD, with one daughter cell being converted into an iN precursor and the other one remaining as a fibroblast. Inhibition of ACD significantly inhibited iN conversion. Moreover, the daughter cells showed asymmetric DNA segregation and histone marks during cytokinesis, and the cells inheriting newly replicated DNA strands during ACD became iN precursors. These results unravel a deterministic step at the early phase of cell reprogramming and demonstrate a novel role of ACD in cell phenotype change. This work also supports a novel hypothesis that daughter cells with newly replicated DNA strands are elite cells for reprogramming, which remains to be tested in various reprogramming processes.
Keywords	asymmetric cell division ; cell fate determination ; direct reprogramming ; epigenetic state ; Science ; Q
Subject code	612
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Asymmetric Cell Division of Fibroblasts is An Early Deterministic Step to Generate Elite Cells during Cell Reprogramming.

Song, Yang / Soto, Jennifer / Wang, Pingping / An, Qin / Zhang, Xuexiang / Hong, SoonGweon / Lee, Luke P / Fan, Guoping / Yang, Li / Li, Song

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2021 Volume 8, Issue 7, Page(s) 2003516

Abstract: Cell reprogramming is considered a stochastic process, and it is not clear which cells are prone to be reprogrammed and whether a deterministic step exists. Here, asymmetric cell division (ACD) at the early stage of induced neuronal (iN) reprogramming is ...

Abstract	Cell reprogramming is considered a stochastic process, and it is not clear which cells are prone to be reprogrammed and whether a deterministic step exists. Here, asymmetric cell division (ACD) at the early stage of induced neuronal (iN) reprogramming is shown to play a deterministic role in generating elite cells for reprogramming. Within one day, fibroblasts underwent ACD, with one daughter cell being converted into an iN precursor and the other one remaining as a fibroblast. Inhibition of ACD significantly inhibited iN conversion. Moreover, the daughter cells showed asymmetric DNA segregation and histone marks during cytokinesis, and the cells inheriting newly replicated DNA strands during ACD became iN precursors. These results unravel a deterministic step at the early phase of cell reprogramming and demonstrate a novel role of ACD in cell phenotype change. This work also supports a novel hypothesis that daughter cells with newly replicated DNA strands are elite cells for reprogramming, which remains to be tested in various reprogramming processes.
MeSH term(s)	Animals ; Asymmetric Cell Division/physiology ; Cellular Reprogramming/physiology ; Fibroblasts/physiology ; Mice ; Mice, Inbred C57BL ; Models, Animal
Language	English
Publishing date	2021-02-25
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202003516
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish.

Hong, SoonGweon / Lee, Philip / Baraban, Scott C / Lee, Luke P

Scientific reports

2016 Volume 6, Page(s) 28248

Abstract: Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for ...

Abstract	Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish.
MeSH term(s)	Animals ; Anticonvulsants/pharmacology ; Anticonvulsants/therapeutic use ; Electrophysiological Phenomena ; Epilepsy/drug therapy ; Epilepsy/physiopathology ; Microelectrodes ; Microfluidics ; Zebrafish/physiology
Chemical Substances	Anticonvulsants
Language	English
Publishing date	2016-06-16
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep28248
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Nanophotonic Cell Lysis and Polymerase Chain Reaction with Gravity-Driven Cell Enrichment for Rapid Detection of Pathogens.

Cho, Byungrae / Lee, Sang Hun / Song, Jihwan / Bhattacharjee, Saptati / Feng, Jeffrey / Hong, SoonGweon / Song, Minsun / Kim, Wonseok / Lee, Jonghwan / Bang, Doyeon / Wang, Bowen / Riley, Lee W / Lee, Luke P

ACS nano

2019 Volume 13, Issue 12, Page(s) 13866–13874

Abstract: Rapid and precise detection of pathogens is a critical step in the prevention and identification of emergencies related to health and biosafety as well as the clinical management of community-acquired urinary tract infections or sexually transmitted ... ...

Abstract	Rapid and precise detection of pathogens is a critical step in the prevention and identification of emergencies related to health and biosafety as well as the clinical management of community-acquired urinary tract infections or sexually transmitted diseases. However, a conventional culture-based pathogen diagnostic method is time-consuming, permitting physicians to use antibiotics without ample clinical data. Here, we present a nanophotonic
MeSH term(s)	Escherichia coli/isolation & purification ; Gravitation ; Nanoparticles/chemistry ; Photons ; Polymerase Chain Reaction/methods
Language	English
Publishing date	2019-12-04
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	1936-086X
ISSN (online)	1936-086X
DOI	10.1021/acsnano.9b04685
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Article ; Online: Preclinical Animal Models for Dravet Syndrome

Aliesha Griffin / Kyla R. Hamling / SoonGweon Hong / Mana Anvar / Luke P. Lee / Scott C. Baraban

Frontiers in Pharmacology, Vol

Seizure Phenotypes, Comorbidities and Drug Screening

2018 Volume 9

Abstract	Epilepsy is a common chronic neurological disease affecting almost 3 million people in the United States and 50 million people worldwide. Despite availability of more than two dozen FDA-approved anti-epileptic drugs (AEDs), one-third of patients fail to receive adequate seizure control. Specifically, pediatric genetic epilepsies are often the most severe, debilitating and pharmaco-resistant forms of epilepsy. Epileptic syndromes share a common symptom of unprovoked seizures. While some epilepsies/forms of epilepsy are the result of acquired insults such as head trauma, febrile seizure, or viral infection, others have a genetic basis. The discovery of epilepsy associated genes suggests varied underlying pathologies and opens the door for development of new “personalized” treatment options for each genetic epilepsy. Among these, Dravet syndrome (DS) has received substantial attention for both the pre-clinical and early clinical development of novel therapeutics. Despite these advances, there is no FDA-approved treatment for DS. Over 80% of patients diagnosed with DS carry a de novo mutation within the voltage-gated sodium channel gene SCN1A and these patients suffer with drug resistant and life-threatening seizures. Here we will review the preclinical animal models for DS featuring inactivation of SCN1A (including zebrafish and mice) with an emphasis on seizure phenotypes and behavioral comorbidities. Because many drugs fail somewhere between initial preclinical discovery and clinical trials, it is equally important that we understand how these models respond to known AEDs. As such, we will also review the available literature and recent drug screening efforts using these models with a focus on assay protocols and predictive pharmacological profiles. Validation of these preclinical models is a critical step in our efforts to efficiently discover new therapies for these patients. The behavioral and electrophysiological drug screening assays in zebrafish will be discussed in detail including specific examples from ...
Keywords	epilepsy ; dravet syndrome ; drug discovery ; in vivo ; precision medicine ; antiepileptic drugs ; Therapeutics. Pharmacology ; RM1-950
Subject code	616
Language	English
Publishing date	2018-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Bubble-free rapid microfluidic PCR

Lee, Sang Hun / Jihwan Song / Byungrae Cho / SoonGweon Hong / Ori Hoxha / Taewook Kang / Dongchoul Kim / Luke P. Lee

Biosensors & bioelectronics. 2019 Feb. 01, v. 126

2019

Abstract: Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble ... ...

Abstract	Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble generation and reagent evaporation, which can cause malfunctions. Here, through theoretical modeling and characterization of bubble behavior, we propose a bubble-free microfluidic PCR device via controlled fluid transfer. Our approach exploits a thin impermeable polyethylene (PE) top layer that minimizes the generation of bubbles by inhibiting mass transport along a vertical direction. Simulation results demonstrate that a calculated mass flow difference of approximately 370% can be obtained by utilizing an impermeable membrane as the vertical barrier layer. To demonstrate proof-of-concept, two nanoporous polymeric materials, poly(dimethylsiloxane) (PDMS) and PE, were used for stand-alone self-powered sample loading (approximately 70 s) and for use as a vertical barrier layer, respectively. Consequently, we demonstrate successful amplification of the cMET gene, a nucleic acid (NA) biomarker for lung cancer, and complete an ultrafast PCR test in less than 3 min using a high powered Peltier-based thermal cycler under bubble-free conditions. This approach will result in a new paradigm for ultrafast molecular diagnosis and can facilitate NA-based nearly instantaneous diagnostics for point-of-care testing and for personalized and preventive medicine.
Keywords	biomarkers ; biosensors ; bubbles ; diagnostic techniques ; evaporation ; gene amplification ; genes ; lung neoplasms ; mass flow ; mass transfer ; medicine ; nanopores ; nucleic acids ; point-of-care testing ; polyethylene ; polymerase chain reaction
Language	English
Dates of publication	2019-0201
Size	p. 725-733.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2018.10.005
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Bubble-free rapid microfluidic PCR.

Lee, Sang Hun / Song, Jihwan / Cho, Byungrae / Hong, SoonGweon / Hoxha, Ori / Kang, Taewook / Kim, Dongchoul / Lee, Luke P

Biosensors & bioelectronics

2018 Volume 126, Page(s) 725–733

Abstract	Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble generation and reagent evaporation, which can cause malfunctions. Here, through theoretical modeling and characterization of bubble behavior, we propose a bubble-free microfluidic PCR device via controlled fluid transfer. Our approach exploits a thin impermeable polyethylene (PE) top layer that minimizes the generation of bubbles by inhibiting mass transport along a vertical direction. Simulation results demonstrate that a calculated mass flow difference of approximately 370% can be obtained by utilizing an impermeable membrane as the vertical barrier layer. To demonstrate proof-of-concept, two nanoporous polymeric materials, poly(dimethylsiloxane) (PDMS) and PE, were used for stand-alone self-powered sample loading (approximately 70 s) and for use as a vertical barrier layer, respectively. Consequently, we demonstrate successful amplification of the cMET gene, a nucleic acid (NA) biomarker for lung cancer, and complete an ultrafast PCR test in less than 3 min using a high powered Peltier-based thermal cycler under bubble-free conditions. This approach will result in a new paradigm for ultrafast molecular diagnosis and can facilitate NA-based nearly instantaneous diagnostics for point-of-care testing and for personalized and preventive medicine.
MeSH term(s)	Biomarkers, Tumor/genetics ; Biomarkers, Tumor/isolation & purification ; Biosensing Techniques ; Computer Simulation ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/genetics ; Microfluidic Analytical Techniques ; Models, Theoretical ; Polyethylene/chemistry ; Polymers/chemistry ; Proto-Oncogene Proteins c-met/genetics ; Proto-Oncogene Proteins c-met/isolation & purification
Chemical Substances	Biomarkers, Tumor ; Polymers ; Polyethylene (9002-88-4) ; MET protein, human (EC 2.7.10.1) ; Proto-Oncogene Proteins c-met (EC 2.7.10.1)
Language	English
Publishing date	2018-10-11
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2018.10.005
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