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  1. Book: Fujian Sheng zheng xie wen shi zi liao xuan bian

    Huang, Youlin

    (Min Tai Zhong yi yao wen hua cong shu)

    2015  

    Title variant Yi jia lei
    Author's details Huang Youlin zhu bian
    Series title Min Tai Zhong yi yao wen hua cong shu
    MeSH term(s) Physicians ; Medicine, Chinese Traditional ; Medicine, Chinese Traditional/history
    Keywords China
    Language Chinese
    Size 4, 238 pages.
    Edition Di 1 ban.
    Document type Book
    ISBN 9787561556306 ; 7561556306
    Database Catalogue of the US National Library of Medicine (NLM)

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  2. Article ; Online: Detection of Circulating Tumor Cell-Related Markers in Gynecologic Cancer Using Microfluidic Devices: A Pilot Study.

    Law, Kim-Seng / Huang, Chung-Er / Chen, Sheng-Wen

    International journal of molecular sciences

    2023  Volume 24, Issue 3

    Abstract: The detection of circulating tumor cells (CTCs) is an emerging strategy for the early detection, prognostication, and identification of recurrent cancer. The clinical utility of CTC detection has been established, but few studies have employed this ... ...

    Abstract The detection of circulating tumor cells (CTCs) is an emerging strategy for the early detection, prognostication, and identification of recurrent cancer. The clinical utility of CTC detection has been established, but few studies have employed this strategy for the detection of gynecologic cancers. Here, we present a novel, biochip-based microfluidic device for the detection of CTCs in gynecologic cancers. The study cohort included three patients with cervical cancer, eight with endometrial cancer, two with ovarian cancer, two with breast cancer, and one with vaginal small cell carcinoma. Four cancer type-specific molecular markers (PanCK, GATA3, HER2, and HE4), as well as CD13, were used for prognostication and recurrence detection, along with downstream genomic analysis. GATA3 and HER2 were markedly expressed in the patients with cervical cancer, and this expression was strongly correlated with the early detection of recurrent disease. All four molecular markers were expressed preoperatively in the patients with endometrial cancer, and the re-expression of different markers was observed at follow-up before recurrence was confirmed. CD13 was identified as an alternative prognostic marker for both cervical and endometrial cancer. Our pilot study indicated that the novel CTC detection system can be used for prognostication and early detection of disease recurrence, which needed further investigation.
    MeSH term(s) Humans ; Female ; Neoplastic Cells, Circulating/pathology ; Microfluidics ; Pilot Projects ; Uterine Cervical Neoplasms ; Neoplasm Recurrence, Local/diagnosis ; Genital Neoplasms, Female/diagnosis ; Breast Neoplasms/metabolism ; Endometrial Neoplasms/diagnosis ; Endometrial Neoplasms/pathology ; Biomarkers, Tumor
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2023-01-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032300
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book: Wen zi sheng yun xun gu bi ji

    Huang, Kan / Huang, Chao

    1983  

    Author's details Huang kan shu; huang chao bian
    Language Chinese
    Size 5, 16, 288 p, 20 cm
    Edition Di 1 ban
    Publisher Shang hai gu ji chu ban she
    Publishing place Shang hai
    Document type Book
    Note Reproduced from manuscript copy
    Database Former special subject collection: coastal and deep sea fishing

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  4. Article: Contributions of Genetic Evolution to Zika Virus Emergence.

    Hung, Su-Jhen / Huang, Sheng-Wen

    Frontiers in microbiology

    2021  Volume 12, Page(s) 655065

    Abstract: Mosquito-borne Zika virus (ZIKV) was considered an obscure virus causing only mild or self-limited symptoms until the explosive outbreaks in French Polynesia in 2013-2014 and in the Americas in 2015-2016, resulting in more than 700,000 cases of the ... ...

    Abstract Mosquito-borne Zika virus (ZIKV) was considered an obscure virus causing only mild or self-limited symptoms until the explosive outbreaks in French Polynesia in 2013-2014 and in the Americas in 2015-2016, resulting in more than 700,000 cases of the disease, with occasional miscarriage and severe congenital birth defects, such as intrauterine growth restriction, fetal microcephaly, and other neurodevelopmental malformations. In this review, we summarized the evolution of ZIKV from a mundane virus to an epidemic virus. ZIKV has acquired a panel of amino acid substitutions during evolution when the virus spread from Africa, Asia, Pacific, through to the Americas. Robust occurrence of mutations in the evolution of ZIKV has increased its epidemic potential. Here we discussed the contributions of these evolutionary mutations to the enhancement of viral pathogenicity and host-mosquito transmission. We further explored the potential hypotheses for the increase in ZIKV activity in recent decades. Through this review, we also explored the hypotheses for the occurrence of the recent ZIKV epidemics and highlighted the potential roles of various factors including pathogen-, host-, vector-related, and environmental factors, which may have synergistically contributed to the ZIKV epidemics.
    Language English
    Publishing date 2021-05-06
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.655065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Detection of Circulating Tumor Cell-Related Markers in Gynecologic Cancer Using Microfluidic Devices

    Kim-Seng Law / Chung-Er Huang / Sheng-Wen Chen

    International Journal of Molecular Sciences, Vol 24, Iss 2300, p

    A Pilot Study

    2023  Volume 2300

    Abstract: The detection of circulating tumor cells (CTCs) is an emerging strategy for the early detection, prognostication, and identification of recurrent cancer. The clinical utility of CTC detection has been established, but few studies have employed this ... ...

    Abstract The detection of circulating tumor cells (CTCs) is an emerging strategy for the early detection, prognostication, and identification of recurrent cancer. The clinical utility of CTC detection has been established, but few studies have employed this strategy for the detection of gynecologic cancers. Here, we present a novel, biochip-based microfluidic device for the detection of CTCs in gynecologic cancers. The study cohort included three patients with cervical cancer, eight with endometrial cancer, two with ovarian cancer, two with breast cancer, and one with vaginal small cell carcinoma. Four cancer type-specific molecular markers (PanCK, GATA3, HER2, and HE4), as well as CD13, were used for prognostication and recurrence detection, along with downstream genomic analysis. GATA3 and HER2 were markedly expressed in the patients with cervical cancer, and this expression was strongly correlated with the early detection of recurrent disease. All four molecular markers were expressed preoperatively in the patients with endometrial cancer, and the re-expression of different markers was observed at follow-up before recurrence was confirmed. CD13 was identified as an alternative prognostic marker for both cervical and endometrial cancer. Our pilot study indicated that the novel CTC detection system can be used for prognostication and early detection of disease recurrence, which needed further investigation.
    Keywords BioChips ; breast cancer ; circulating tumor cells ; cervical cancer ; ovarian cancer ; endometrial cancer ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 610 ; 616
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  6. Article: High-resolution magnetic resonance vessel wall imaging in ischemic stroke and carotid artery atherosclerotic stenosis: A review.

    Huang, Li-Xin / Wu, Xiao-Bing / Liu, Yi-Ao / Guo, Xin / Liu, Chi-Chen / Cai, Wang-Qing / Wang, Sheng-Wen / Luo, Bin

    Heliyon

    2024  Volume 10, Issue 7, Page(s) e27948

    Abstract: Ischemic stroke is a significant burden on human health worldwide. Carotid Atherosclerosis stenosis plays an important role in the comprehensive assessment and prevention of ischemic stroke patients. High-resolution vessel wall magnetic resonance imaging ...

    Abstract Ischemic stroke is a significant burden on human health worldwide. Carotid Atherosclerosis stenosis plays an important role in the comprehensive assessment and prevention of ischemic stroke patients. High-resolution vessel wall magnetic resonance imaging has emerged as a successful technique for assessing carotid atherosclerosis stenosis. This advanced imaging modality has shown promise in effectively displaying a wide range of characteristics associated with the condition, leading to a comprehensive evaluation. High-resolution vessel wall magnetic resonance imaging not only enables a comprehensive evaluation of the instability of carotid atherosclerosis stenosis plaques but also provides valuable information for understanding the pathogenesis and predicting the prognosis of ischemic stroke patients. The purpose of this article is to review the application of high-resolution magnetic resonance imaging in ischemic stroke and carotid atherosclerotic stenosis.
    Language English
    Publishing date 2024-03-24
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e27948
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: VP1 codon deoptimization and high-fidelity substitutions in 3D polymerase as potential vaccine strategies for eliciting immune responses against enterovirus A71.

    Hsieh, Wen-Sheng / Chao, Chiao-Hsuan / Shen, Chun-Yu / Cheng, Dayna / Huang, Sheng-Wen / Wang, Ya-Fang / Chen, Chien-Chin / Chen, Shun-Hua / Hsu, Li-Jin / Wang, Jen-Ren

    Journal of virology

    2024  Volume 98, Issue 1, Page(s) e0155823

    Abstract: Enterovirus A71 (EV-A71) can induce severe neurological complications and even fatal encephalitis in children, and it has caused several large outbreaks in Taiwan since 1998. We previously generated VP1 codon-deoptimized (VP1-CD) reverse genetics (rg) EV- ...

    Abstract Enterovirus A71 (EV-A71) can induce severe neurological complications and even fatal encephalitis in children, and it has caused several large outbreaks in Taiwan since 1998. We previously generated VP1 codon-deoptimized (VP1-CD) reverse genetics (rg) EV-A71 viruses (rgEV-A71s) that harbor a high-fidelity (HF) 3D polymerase. These VP1-CD-HF rgEV-A71s showed lower replication kinetics
    MeSH term(s) Animals ; Mice ; Antibodies, Viral/immunology ; Codon ; Enterovirus A, Human/genetics ; Enterovirus Infections/immunology ; Vaccines, Attenuated ; Capsid Proteins/genetics ; Immunity, Humoral ; Immunity, Cellular ; Antibodies, Neutralizing/immunology ; Viral Vaccines ; Mice, Inbred ICR ; Mice, Inbred BALB C ; RNA-Dependent RNA Polymerase/genetics
    Chemical Substances Antibodies, Viral ; Codon ; Vaccines, Attenuated ; Capsid Proteins ; Antibodies, Neutralizing ; Viral Vaccines ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
    Language English
    Publishing date 2024-01-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.01558-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Antigenic mapping of enterovirus A71 from Taiwan and Southeast Asia.

    Cheng, Dayna / Huang, Sheng-Wen / Tsai, Yi-Hsuan / Lien, Yun-Yin / Wang, Jen-Ren

    Antiviral research

    2023  Volume 212, Page(s) 105569

    Abstract: Enterovirus A71 (EV-A71) is a non-enveloped virus possessing 4 capsid proteins: VP1-VP4. The outermost capsid protein, VP1, plays roles in both antigenicity and virulence of the virus. The concept of generating other EV-A71 genotypes of reverse genetics ( ...

    Abstract Enterovirus A71 (EV-A71) is a non-enveloped virus possessing 4 capsid proteins: VP1-VP4. The outermost capsid protein, VP1, plays roles in both antigenicity and virulence of the virus. The concept of generating other EV-A71 genotypes of reverse genetics (rg) viruses by replacing VP1 can be made possible with synthetic biotechnology, allowing us to redesign organisms, creating unavailable ones. To determine suitable vaccine candidates against EV-A71 infections, we combined synthetic biotechnology, rg-virus production and high-fidelity determinants to produce genetically stable viruses. With the use of antigenic cartography, we are able to view the antigenic distance among various points. We analyzed and generated various EV-A71 VP1 sequences from Taiwan and Southeast Asian (SEA) countries, which were then used to produce recombinant rg-viruses and the viral proteins were purified for immunization of mice and rabbits. Antisera against various EV-A71 genotypes were used in neutralization assays against various Taiwan and SEA EV-A71 genotypes. Based on neutralization data from mice and rabbit antisera, we found that antisera produced from several genotypes were able to effectively neutralize the various Taiwan and SEA EV-A71 genotypes. Additionally, comparing the antigenic maps produced from mouse, rabbit and human antisera against different EV-A71 genotypes, a difference in clustering was seen and the spacing between points also differed. Based on antigenic mapping and neutralizing activities, B4 7008-HF and C4 M79 may be good potential vaccine candidates against EV-A71.
    MeSH term(s) Animals ; Humans ; Rabbits ; Enterovirus/genetics ; Enterovirus A, Human/genetics ; Taiwan ; Enterovirus Infections ; Antigens, Viral/genetics ; Capsid Proteins/metabolism
    Chemical Substances Antigens, Viral ; Capsid Proteins
    Language English
    Publishing date 2023-02-22
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2023.105569
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1627: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Conserved Residues Adjacent to ß-Barrel and Loop Intersection among Enterovirus VP1 Affect Viral Replication: Potential Target for Anti-Enteroviral Development.

    Huang, Ya-Ling / Huang, Sheng-Wen / Shen, Chun-Yu / Cheng, Dayna / Wang, Jen-Ren

    Viruses

    2022  Volume 14, Issue 2

    Abstract: ... ...

    Abstract Enterovirus
    MeSH term(s) Amino Acid Sequence ; Antiviral Agents/chemistry ; Capsid Proteins/chemistry ; Capsid Proteins/genetics ; Cell Line, Tumor ; Conserved Sequence ; Enterovirus/physiology ; Humans ; Mutation ; Protein Conformation ; Protein Stability ; RNA, Viral/metabolism ; Small Molecule Libraries/chemistry ; Temperature ; Viral Load ; Virus Replication
    Chemical Substances Antiviral Agents ; Capsid Proteins ; RNA, Viral ; Small Molecule Libraries
    Language English
    Publishing date 2022-02-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020364
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Conserved Residues Adjacent to ß-Barrel and Loop Intersection among Enterovirus VP1 Affect Viral Replication: Potential Target for Anti-Enteroviral Development

    Huang, Ya-Ling / Huang, Sheng-Wen / Shen, Chun-Yu / Cheng, Dayna / Wang, Jen-Ren

    Viruses. 2022 Feb. 10, v. 14, no. 2

    2022  

    Abstract: Enterovirus genus has over one hundred genotypes and could cause several kinds of severe animal and human diseases. Understanding the role of conserved residues in the VP1 capsid protein among the enterovirus genus may lead to anti-enteroviral drug ... ...

    Abstract Enterovirus genus has over one hundred genotypes and could cause several kinds of severe animal and human diseases. Understanding the role of conserved residues in the VP1 capsid protein among the enterovirus genus may lead to anti-enteroviral drug development. The highly conserved residues were found to be located at the loop and ß-barrel intersections. To elucidate the role of these VP1 residues among the enterovirus genus, alanine substitution reverse genetics (rg) variants were generated, and virus properties were investigated for their impact. Six highly conserved residues were identified as located near the inside of the canyon, and four of them were close to the ß-barrel and loop intersection. The variants rgVP1-R86A, rgVP1-P193A, rgVP1-G231A, and rgVP1-K256A were unable to be obtained, which may be due to disruption in the virus replication process. In contrast, rgVP1-E134A and rgVP1-P157A replicated well and rgVP1-P157A showed smaller plaque size, lower viral growth kinetics, and thermal instability at 39.5°C when compared to the rg wild type virus. These findings showed that the conserved residues located at the ß-barrel and loop junction play roles in modulating viral replication, which may provide a pivotal role for pan-enteroviral inhibitor candidate.
    Keywords Enterovirus ; alanine ; coat proteins ; drug development ; growth models ; humans ; reverse genetics ; viral growth ; virus replication ; viruses
    Language English
    Dates of publication 2022-0210
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020364
    Database NAL-Catalogue (AGRICOLA)

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