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Article: Migration mechanism and risk assessment of chlorinated paraffins in highly polluted Ya’Er lake area, China

Li, Chang / Chen, Lufeng / He, Yujian / Liang, Yong / Wang, Yingjun / Li, Feifei / Gao, Wei / Wang, Yawei / Jiang, Guibin

Environmental pollution. 2021 July 15, v. 281

2021

Abstract: Chlorinated paraffins (CPs), a type of toxic and persistent organic substances, can persist in environmental media for a long time and have adverse effects on human health. Thus, it is of great importance to investigate the accumulation and environmental ...

Abstract	Chlorinated paraffins (CPs), a type of toxic and persistent organic substances, can persist in environmental media for a long time and have adverse effects on human health. Thus, it is of great importance to investigate the accumulation and environmental behavior of CPs in industrial areas. In this study, farmland soil, water, and sediment core samples from abandoned oxidation ponds used by three chemical plants to treat wastewater over the past 38 years were investigated in detail. Results show that the concentration of CPs in sediments varied significantly with the water flow direction. The oxidation pond closest to a sewage outlet had the highest concentrations of short-chain chlorinated paraffin (SCCPs) and medium-chain chlorinated paraffin (MCCPs), within the ranges of 44.0–6.21 × 10⁴ ng/g dw (mean 9.32 × 10³ ng/g dw) and 143–1.30 × 10⁶ ng/g dw (mean 1.22 × 10⁵ ng/g dw), respectively. However, in the oxidation pond farthest from the sewage outlet, CP concentrations in sediments were significantly reduced, with ∑SCCPs and ∑MCCPs concentrations ranging from N.D.-249 ng/g dw (mean 66.8 ng/g dw) and N.D.-222 ng/g dw (mean 34.0 ng/g dw), respectively. Moreover, MCCP level in the water was below the detection limit, while the concentration of SCCP ranged from 41.0 to 1.53 × 10³ ng/L (mean 267 ng/L). Finally, a remarkable spatial trend and specific congener distribution were observed in the sediment test results. The horizontal and vertical distributions of the sediments indicate that short-chain (C₁₀₋₁₁) and low-chlorinated (Cl₆₋₇) homologs are more likely to migrate deeper or farther away from the pollution source.
Keywords	agricultural soils ; detection limit ; human health ; lakes ; oxidation ; pollution ; risk assessment ; sediments ; sewage ; toxicity ; wastewater ; water flow ; China
Language	English
Dates of publication	2021-0715
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	280652-6
ISSN	1873-6424 ; 0013-9327 ; 0269-7491
ISSN (online)	1873-6424
ISSN	0013-9327 ; 0269-7491
DOI	10.1016/j.envpol.2021.117015
Database	NAL-Catalogue (AGRICOLA)

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Article: The effect of storage time on tea Polyphenols, catechin compounds, total flavones and the biological activity of Ya’an Tibetan tea (Camellia sinensis)

Zheng, Qiaoran / Li, Wenfeng / Gao, Xiaoxv

Journal of food processing and preservation. 2021 Dec., v. 45, no. 12

2021

Abstract: The content of tea polyphenols (TPP), tea total flavones (TFS) and tea catechin compounds in Ya ... antioxidative activity of tea were measured. Moreover, the correlation between the levels of tea catechins in Ya ... increased significantly. Five catechins were identified in Ya’an Tibetan tea using UPLC‐QqQ‐MS/MS, and ...

Abstract	The content of tea polyphenols (TPP), tea total flavones (TFS) and tea catechin compounds in Ya’an Tibetan tea after different storage times was investigated, and the α‐glucosidase inhibition activity and antioxidative activity of tea were measured. Moreover, the correlation between the levels of tea catechins in Ya’an Tibetan tea with different storage years and their bioactivities were evaluated. During the period of storage, the content of Tibetan tea polyphenols decreased, and the content of Tibetan tea flavonoids increased significantly. Five catechins were identified in Ya’an Tibetan tea using UPLC‐QqQ‐MS/MS, and the Ya’an Tibetan tea had diverse antioxidant activities and α‐glucosidase inhibitory activities over different storage years, due to the various tea catechin components. (‐)‐epigallocatechin gallate (EGCG), (‐)‐epicatechin gallate (ECG), and (‐)‐epicatechin (EC) were found to be responsible for its strong antioxidant activity and α‐glucosidase inhibitory activity, indicating Ya’an Tibetan tea has potential as a functional food. PRACTICAL APPLICATIONS: Ya’an Tibetan tea, also called as Kangzhuan brick tea, is a typical Chinese dark tea with a dark brown color. The change of the main active substances and its bioactivity in Ya’an Tibetan tea during a long‐term storage was obtained in this study. These findings have provided important reference basis for people to have a better understanding of the chemical constituents and bioactivity of Ya’an Tibetan tea which store for a long time. Moreover, our study showed Ya’an Tibetan tea has high antioxidant activity and α‐glucosidase inhibitory activity. These findings will improve product quality and upgrade Ya’an Tibetan tea industry, and promotes Tibetan tea economic development.
Keywords	Camellia sinensis ; antioxidant activity ; antioxidants ; catechin ; color ; economic development ; epigallocatechin gallate ; flavones ; functional foods ; industry ; people ; polyphenols ; product quality ; storage time ; tea
Language	English
Dates of publication	2021-12
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	243448-9
ISSN	1745-4549 ; 0145-8892
ISSN (online)	1745-4549
ISSN	0145-8892
DOI	10.1111/jfpp.16004
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Article ; Online: NF-YA promotes invasion and angiogenesis by upregulating EZH2-STAT3 signaling in human melanoma cells.

Xu, Zihan / Sun, Yaowen / Guo, Yadong / Qin, Gaoping / Mu, Shengzhi / Fan, Ronghui / Wang, Benfeng / Gao, Wenjie / Wu, Hangli / Wang, Guodong / Zhang, Zhenxin

Oncology reports

2016 Volume 35, Issue 6, Page(s) 3630–3638

Abstract: ... angiogenesis. Here, we found that overexpression of nuclear transcription factor-Y alpha (NF-YA) gene ... by the overexpression of NF-YA gene in melanoma cells. The STAT3 inhibitor was able to attenuate the upregulation ... of VEGF induced by NF-YA overexpression. Enhancer of zeste homolog 2 (EZH2), the catalytic subunit ...

Abstract	The process of angiogenesis is essential for tumor development and metastasis. Vascular endothelial growth factor (VEGF), which is overexpressed in most human cancers, has been demonstrated to be a major modulator of angiogenesis. Thus, inhibition of VEGF signaling has the potential for tumor anti-angiogenic therapy. Signal transducer and activator of transcription-3 (STAT3) is a key regulator for angiogenesis by directly binding to the VEGF promoter to upregulate its transcription. Several factors can enhance STAT3 activity to affect angiogenesis. Here, we found that overexpression of nuclear transcription factor-Y alpha (NF-YA) gene could promote cell invasion and angiogenesis accompanying the increase of STAT3 signaling in human melanoma cells. Moreover, the expression and secretion of VEGF was also found to be upregulated by the overexpression of NF-YA gene in melanoma cells. The STAT3 inhibitor was able to attenuate the upregulation of VEGF induced by NF-YA overexpression. Enhancer of zeste homolog 2 (EZH2), the catalytic subunit of the Polycomb repressive complex 2, enhances STAT3 activity by mediating its lysine methylation. We also showed that NF-YA upregulated the expression of EZH2 and NF-YA‑induced angiogenesis could be inhibited by EZH2 knockdown. Taken together, these findings indicate that overexpression of NF-YA contributes to tumor angiogenesis through EZH2-STAT3 signaling in human melanoma cells, highlighting NF-YA as a potential therapeutic target in human melanoma.
MeSH term(s)	CCAAT-Binding Factor/metabolism ; Cell Line, Tumor ; Enhancer of Zeste Homolog 2 Protein/metabolism ; HEK293 Cells ; Human Umbilical Vein Endothelial Cells ; Humans ; Melanoma/pathology ; Methylation ; Neoplasm Invasiveness/pathology ; Neovascularization, Pathologic/pathology ; STAT3 Transcription Factor/metabolism ; Signal Transduction ; Vascular Endothelial Growth Factor A/metabolism ; Vascular Endothelial Growth Factor A/secretion
Chemical Substances	CCAAT-Binding Factor ; NFYA protein, human ; STAT3 Transcription Factor ; STAT3 protein, human ; VEGFA protein, human ; Vascular Endothelial Growth Factor A ; EZH2 protein, human (EC 2.1.1.43) ; Enhancer of Zeste Homolog 2 Protein (EC 2.1.1.43)
Language	English
Publishing date	2016-06
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1222484-4
ISSN	1791-2431 ; 1021-335X
ISSN (online)	1791-2431
ISSN	1021-335X
DOI	10.3892/or.2016.4761
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Article ; Online: Ya-fish (Schizothorax prenanti) spexin: identification, tissue distribution and mRNA expression responses to periprandial and fasting.

Wu, Hongwei / Lin, Fangjun / Chen, Hu / Liu, Ju / Gao, Yundi / Zhang, Xin / Hao, Jin / Chen, Defang / Yuan, Dengyue / Wang, Tao / Li, Zhiqiong

Fish physiology and biochemistry

2016 Volume 42, Issue 1, Page(s) 39–49

Abstract: ... expression responses to feeding status of SPX in Ya-fish (Schizothorax prenanti). The full-length cDNA of Ya ... that the Ya-fish SPX showed high conservation with other species. Our data revealed that SPX was widely ... expressed in all test tissues. The highest expression of SPX mRNA was observed in Ya-fish forebrain ...

Abstract	Spexin (SPX) is a novel peptide which was known for its role in physiological homeostasis. A recent study has confirmed that SPX plays an important role in the feeding regulation. However, the reports about SPX are very limited. In the present study, we characterized the structure, distribution and mRNA expression responses to feeding status of SPX in Ya-fish (Schizothorax prenanti). The full-length cDNA of Ya-fish SPX was 1330 base pairs (bp), which encoded 106 amino acid residues. These residues contained a 31-amino acid signal peptide region and a 14-amino acid mature peptide. The sequence alignment demonstrated that the Ya-fish SPX showed high conservation with other species. Our data revealed that SPX was widely expressed in all test tissues. The highest expression of SPX mRNA was observed in Ya-fish forebrain. Compared with the Ya-fish SPX mRNA expression in the forebrain between the preprandial and postprandial groups, the fed group was prominently increased than unfed groups after a meal, while the unfed group at 1 and 3 h substantially decreased than preprandial groups (P < 0.01). In addition, SPX mRNA expression in forebrain was significantly decreased (P < 0.01) during fasting for a week and sharply increased (P < 0.01) after refeeding on the 7th day, and then return to normal level on the 9th day. These results point toward that SPX mRNA expression is regulated by metabolic status or feeding conditions in Ya-fish.
MeSH term(s)	Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Cyprinidae/genetics ; Cyprinidae/metabolism ; DNA, Complementary/genetics ; Fasting/metabolism ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Molecular Sequence Data ; Peptide Hormones/genetics ; Peptide Hormones/metabolism ; Phylogeny ; Postprandial Period/physiology ; Prosencephalon/metabolism ; RNA, Messenger/metabolism
Chemical Substances	DNA, Complementary ; Fish Proteins ; Peptide Hormones ; RNA, Messenger
Language	English
Publishing date	2016-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	292907-7
ISSN	1573-5168 ; 0920-1742
ISSN (online)	1573-5168
ISSN	0920-1742
DOI	10.1007/s10695-015-0115-0
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Article ; Online: ZHX2 enhances the cytotoxicity of chemotherapeutic drugs in liver tumor cells by repressing MDR1 via interfering with NF-YA.

Ma, Hongxin / Yue, Xuetian / Gao, Lifen / Liang, Xiaohong / Yan, Wenjiang / Zhang, Zhenyu / Shan, Haixia / Zhang, Hualin / Spear, Brett T / Ma, Chunhong

Oncotarget

2015 Volume 6, Issue 2, Page(s) 1049–1063

Abstract: ... promoter activity, while knockdown of NF-YA or mutating the NF-Y binding site eliminated this ZHX2-mediated ... repression of MDR1 transcription. Co-IP and ChIP assay further suggested that ZHX2 interacted with NF-YA and ...

Abstract	We previously reported the tumor suppressor function of Zinc-fingers and homeoboxes 2 (ZHX2) in hepatocellular carcinoma (HCC). Other studies indicate the association of increased ZHX2 expression with improved response to high dose chemotherapy in multiple myeloma. Here, we aim to test whether increased ZHX2 levels in HCC cells repress multidrug resistance 1(MDR1) expression resulting in increased sensitivity to chemotherapeutic drugs. We showed evidence that increased ZHX2 levels correlated with reduced MDR1 expression and enhanced the cytotoxicity of CDDP and ADM in different HCC cell lines. Consistently, elevated ZHX2 significantly reduced ADM efflux in HepG2 cells and greatly increased the CDDP-mediated suppression of liver tumor growth in vivo. Furthermore, immunohistochemical staining demonstrated the inverse correlation of ZHX2 and MDR1 expression in HCC tissues. Luciferase report assay showed that ZHX2 repressed the MDR1 promoter activity, while knockdown of NF-YA or mutating the NF-Y binding site eliminated this ZHX2-mediated repression of MDR1 transcription. Co-IP and ChIP assay further suggested that ZHX2 interacted with NF-YA and reduced NF-Y binding to the MDR1 promoter. Taken together, we clarify that ZHX2 represses NF-Y-mediated activation of MDR1 transcription and, in doing so, enhances the effects of chemotherapeutics in HCC cells both in vitro and in vivo.
MeSH term(s)	ATP Binding Cassette Transporter, Sub-Family B/genetics ; ATP Binding Cassette Transporter, Sub-Family B/metabolism ; Adult ; Aged ; Animals ; Antineoplastic Agents/pharmacology ; CCAAT-Binding Factor/genetics ; CCAAT-Binding Factor/metabolism ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/metabolism ; Cell Line, Tumor ; Cell Survival/drug effects ; Cell Survival/genetics ; Cisplatin/pharmacology ; Female ; HEK293 Cells ; Hep G2 Cells ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Immunohistochemistry ; Liver Neoplasms/drug therapy ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism ; Male ; Mice, Inbred BALB C ; Mice, Nude ; Middle Aged ; Protein Binding ; RNA Interference ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Xenograft Model Antitumor Assays
Chemical Substances	ABCB1 protein, human ; ATP Binding Cassette Transporter, Sub-Family B ; Antineoplastic Agents ; CCAAT-Binding Factor ; Homeodomain Proteins ; NFYA protein, human ; Transcription Factors ; ZHX2 protein, human ; Cisplatin (Q20Q21Q62J)
Language	English
Publishing date	2015-01-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.2832
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Article ; Online: Molecular characterization, tissue distribution and feeding related changes of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti).

Lin, Fangjun / Zhou, Chaowei / Chen, Hu / Wu, Hongwei / Xin, Zhiming / Liu, Ju / Gao, Yundi / Yuan, Dengyue / Wang, Tao / Wei, Rongbin / Chen, Defang / Yang, Shiyong / Wang, Yan / Pu, Yundan / Li, Zhiqiong

Gene

2014 Volume 536, Issue 2, Page(s) 238–246

Abstract: ... we characterized the structure, distribution, and meal responsive of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax ... prenanti) for the first time. The full length cDNA of Ya-fish was 2140base pair (bp), which encoded ... among other species is not present in Ya-fish NUCB2A sequence. Tissue distribution analysis revealed that Ya-fish ...

Abstract	The protein nucleobindin-2 (NUCB2) was identified over a decade ago and recently raised great interest as its derived peptide nesfatin-1 was shown to reduce food intake and body weight in rodents. However, the involvement of NUCB2 in feeding behavior has not well been studied in fish. In the present study, we characterized the structure, distribution, and meal responsive of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti) for the first time. The full length cDNA of Ya-fish was 2140base pair (bp), which encoded a polypeptide of 487 amino acid residues including a 23 amino acid signal peptide. A high conservation in NUCB2 sequences was found in vertebrates, however the proposed propeptide cleavage site (Arg-Arg) conserved among other species is not present in Ya-fish NUCB2A sequence. Tissue distribution analysis revealed that Ya-fish NUCB2A mRNA was ubiquitously expressed in all test tissues, and abundant expression was detected in several regions including the hypothalamus, hepatopancreas, ovary and intestines. NUCB2A mRNA expression respond to feeding status change may vary and be tissue specific. NUCB2A mRNA levels significantly increased (P<0.05) in the hypothalamus and intestines after feeding and substantially decreased (P<0.01) during a week food deprivation in the hypothalamus. Meanwhile, NUCB2A mRNA in the hepatopancreas was significantly elevated (P<0.001) during food deprivation, and a similar increase was also found after short-time fasting. This points toward a potential hepatopancreas specific local role for NUCB2A in the regulation of metabolism during food deprivation. Collectively, these results provide the molecular and functional evidence to support potential anorectic and metabolic roles for NUCB2A in Ya-fish.
MeSH term(s)	Amino Acid Sequence ; Animals ; Base Sequence ; Calcium-Binding Proteins/genetics ; Cloning, Molecular ; DNA, Complementary/genetics ; DNA-Binding Proteins/genetics ; Eating/genetics ; Fishes/genetics ; Molecular Sequence Data ; Nerve Tissue Proteins/genetics ; Phylogeny ; RNA, Messenger/genetics ; Sequence Alignment ; Tissue Distribution/genetics
Chemical Substances	Calcium-Binding Proteins ; DNA, Complementary ; DNA-Binding Proteins ; Nerve Tissue Proteins ; RNA, Messenger ; nucleobindin
Language	English
Publishing date	2014-02-25
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2013.12.031
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Zs.A 1357: Show issues

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Article ; Online: Molecular and physiological evidences for the role in appetite regulation of apelin and its receptor APJ in Ya-fish (Schizothorax prenanti).

Lin, Fangjun / Wu, Hongwei / Chen, Hu / Xin, Zhiming / Yuan, Dengyue / Wang, Tao / Liu, Ju / Gao, Yundi / Zhang, Xin / Zhou, Chaowei / Wei, Rongbin / Chen, Defang / Yang, Shiyong / Wang, Yan / Pu, Yundan / Li, Zhiqiong

Molecular and cellular endocrinology

2014 Volume 396, Issue 1-2, Page(s) 46–57

Abstract: ... in Ya-fish (Schizothorax prenanti) tissues. We also assessed the effects of different nutritional status ... suggest that apelin acts as an orexigenic factor in Ya-fish. Their widespread distributions also suggest ...

Abstract	Apelin is a recently discovered peptide produced by several tissues with diverse physiological actions mediated by its receptor APJ. In order to better understand the role of apelin in the regulation of appetite in fish, we cloned the cDNAs encoding apelin and APJ, and investigated their mRNA distributions in Ya-fish (Schizothorax prenanti) tissues. We also assessed the effects of different nutritional status on apelin and APJ mRNAs abundance. Apelin and APJ mRNAs were ubiquitously expressed in all tissues tested, relatively high expression levels were detected in the heart, spleen, hypothalamus and kidney. Short-term fasting significant increased APJ mRNA expression, but no significant difference between fasted fish and fed control on 5- and 7-day. Meanwhile, apelin mRNA expression consistently increased during the 7-day food deprivation. In order to further characterize apelin in fish, we performed intraperitoneal (i.p.) injection of apelin-13 and examined food intake of the injected fish. Apelin injected at a dose of 100 ng/g body weight induced a significant increase in food intake compared to saline injected fish. Our results suggest that apelin acts as an orexigenic factor in Ya-fish. Their widespread distributions also suggest that apelin and APJ might play multiple physiological regulating roles in fish.
MeSH term(s)	Amino Acid Sequence ; Animals ; Appetite/genetics ; Appetite Regulation/genetics ; Base Sequence ; Cloning, Molecular ; Eating/drug effects ; Eating/genetics ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Fasting ; Fishes/classification ; Fishes/genetics ; Fishes/metabolism ; Food Deprivation ; Gene Expression Regulation ; Hypothalamus/metabolism ; Injections, Intraperitoneal ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Intercellular Signaling Peptides and Proteins/pharmacology ; Kidney/metabolism ; Molecular Sequence Data ; Myocardium/metabolism ; Phylogeny ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Homology, Amino Acid ; Spleen/metabolism
Chemical Substances	Intercellular Signaling Peptides and Proteins ; RNA, Messenger ; Receptors, G-Protein-Coupled ; Recombinant Proteins
Language	English
Publishing date	2014-10
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2014.08.009
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Article: Molecular characterization, tissue distribution and feeding related changes of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti)

Lin, Fangjun / Chaowei Zhou / Defang Chen / Dengyue Yuan / Hongwei Wu / Hu Chen / Ju Liu / Rongbin Wei / Shiyong Yang / Tao Wang / Yan Wang / Yundan Pu / Yundi Gao / Zhiming Xin / Zhiqiong Li

Gene. 2014 Feb. 25, v. 536

2014

Abstract	The protein nucleobindin-2 (NUCB2) was identified over a decade ago and recently raised great interest as its derived peptide nesfatin-1 was shown to reduce food intake and body weight in rodents. However, the involvement of NUCB2 in feeding behavior has not well been studied in fish. In the present study, we characterized the structure, distribution, and meal responsive of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti) for the first time. The full length cDNA of Ya-fish was 2140base pair (bp), which encoded a polypeptide of 487 amino acid residues including a 23 amino acid signal peptide. A high conservation in NUCB2 sequences was found in vertebrates, however the proposed propeptide cleavage site (ArgâArg) conserved among other species is not present in Ya-fish NUCB2A sequence. Tissue distribution analysis revealed that Ya-fish NUCB2A mRNA was ubiquitously expressed in all test tissues, and abundant expression was detected in several regions including the hypothalamus, hepatopancreas, ovary and intestines. NUCB2A mRNA expression respond to feeding status change may vary and be tissue specific. NUCB2A mRNA levels significantly increased (P<0.05) in the hypothalamus and intestines after feeding and substantially decreased (P<0.01) during a week food deprivation in the hypothalamus. Meanwhile, NUCB2A mRNA in the hepatopancreas was significantly elevated (P<0.001) during food deprivation, and a similar increase was also found after short-time fasting. This points toward a potential hepatopancreas specific local role for NUCB2A in the regulation of metabolism during food deprivation. Collectively, these results provide the molecular and functional evidence to support potential anorectic and metabolic roles for NUCB2A in Ya-fish.
Keywords	amino acids ; body weight ; complementary DNA ; fasting ; feeding behavior ; fish ; food deprivation ; food intake ; gene expression ; hepatopancreas ; hypothalamus ; intestines ; messenger RNA ; metabolism ; polypeptides ; rodents ; Schizothorax prenanti ; signal peptide ; tissue distribution
Language	English
Dates of publication	2014-0225
Size	p. 238-246.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2013.12.031
Database	NAL-Catalogue (AGRICOLA)

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Article: Molecular and physiological evidences for the role in appetite regulation of apelin and its receptor APJ in Ya-fish (Schizothorax prenanti)

Lin, Fangjun / Chaowei Zhou / Defang Chen / Dengyue Yuan / Hongwei Wu / Hu Chen / Ju Liu / Rongbin Wei / Shiyong Yang / Tao Wang / Xin Zhang / Yan Wang / Yundan Pu / Yundi Gao / Zhiming Xin / Zhiqiong Li

Molecular and Cellular Endocrinology. 2014 Oct., v. 396

2014

Abstract	Apelin is a recently discovered peptide produced by several tissues with diverse physiological actions mediated by its receptor APJ. In order to better understand the role of apelin in the regulation of appetite in fish, we cloned the cDNAs encoding apelin and APJ, and investigated their mRNA distributions in Ya-fish (Schizothorax prenanti) tissues. We also assessed the effects of different nutritional status on apelin and APJ mRNAs abundance. Apelin and APJ mRNAs were ubiquitously expressed in all tissues tested, relatively high expression levels were detected in the heart, spleen, hypothalamus and kidney. Short-term fasting significant increased APJ mRNA expression, but no significant difference between fasted fish and fed control on 5- and 7-day. Meanwhile, apelin mRNA expression consistently increased during the 7-day food deprivation. In order to further characterize apelin in fish, we performed intraperitoneal (i.p.) injection of apelin-13 and examined food intake of the injected fish. Apelin injected at a dose of 100âng/g body weight induced a significant increase in food intake compared to saline injected fish. Our results suggest that apelin acts as an orexigenic factor in Ya-fish. Their widespread distributions also suggest that apelin and APJ might play multiple physiological regulating roles in fish.
Keywords	appetite ; body weight ; complementary DNA ; fasting ; fish ; food deprivation ; food intake ; gene expression ; heart ; hypothalamus ; kidneys ; messenger RNA ; nutritional status ; Schizothorax prenanti ; spleen ; tissues
Language	English
Dates of publication	2014-10
Size	p. 46-57.
Publishing place	Elsevier Ireland Ltd
Document type	Article
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2014.08.009
Database	NAL-Catalogue (AGRICOLA)

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Book: Gao xue ya Zhong xi yi zhen liao yu tiao yang

Pan, Yi / Wang, Xiaoping / Yang, Yejun / Du, Tongfang / Gao, Hansen

Hypertension

(Zhong xi yi zhen liao yu tiao yang xi lie cong shu)

2000

Title variant	Hypertension
Author's details	Pan Yi, Wang Xiaoping, Yang Yejun bian zhu ; Du Tongfang, Gao Hansen bian shen
Series title	Zhong xi yi zhen liao yu tiao yang xi lie cong shu
MeSH term(s)	Hypertension ; Medicine, Chinese Traditional
Language	Chinese
Size	296 p. :, ill. ;, 21 cm.
Edition	Di 1 ban, jia ting shi yong ban.
Publisher	Guangdong lü you chu ban she
Publishing place	Guangzhou Shi
Document type	Book
Note	Text in Chinese.
ISBN	9787806531815 ; 7806531815
Database	Catalogue of the US National Library of Medicine (NLM)

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