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Article ; Online: Jiu-Wei-Yong-An Formula suppresses JAK1/STAT3 and MAPK signaling alleviates atopic dermatitis-like skin lesions.

Qinwufeng, Gu / Jiacheng, Lin / Xiaoling, Lu / Tingru, Chen / Yunyang, Wu / Yanlong, Yang

2022 Volume 295, Page(s) 115428

Abstract: Ethnopharmacological relevance: Jiu-Wei-Yong-An (JWYA) formula is a traditional Chinese medicine ...

Abstract	Ethnopharmacological relevance: Jiu-Wei-Yong-An (JWYA) formula is a traditional Chinese medicine (TCM) prescription used to treat atopic dermatitis (AD) in the clinic. JWYA is considered to have anti-inflammatory and antipruritic properties. However, the mechanism of JWYA remains unclear. Aim of the study: This study aimed to investigate the effect of JWYA on an experimental mouse AD model. Materials and methods: Mice were sensitized with 2,4-dinitrochlorobenzene (DNCB) and intragastrically administered with JWYA for 14 days. The therapeutic effect was assessed using a grade four dermatitis score, skin moisture, thickness measurements, and a mouse behavior tests. H&E and toluidine blue staining were used to observe epidermal inflammatory thickening and mast cells in mouse skin lesions. Serum IgE levels and skin TNF-α and IL-4 levels were determined using ELISAs. The TNF-α, IL-1β, IL-4, IL-13, IL-31, IL-33, and IFN-γ mRNA expression levels in skin lesions were detected using qPCR. Network pharmacology analysis based on serum active components was performed to elucidate the mechanism, and the results were verified by Western blotting. Finally, we tested the binding affinity between the active ingredients of JWYA and JAK1 via molecular docking. Results: JWYA improved the skin lesions of AD mice, relieved itching and reduced skin thickening. Additionally, JWYA decreased the serum IgE level and the levels of TNF-α, IL-1β, IL-4, IL-13, IL-31, IL-33, and IFN-γ in skin. Moreover, JWYA inhibited the activation of JAK1/STAT3 and MAPK (p38, ERK, and JNK) signaling. Molecular docking showed that kaempferol, luteolin, and forsythin have high affinity for JAK1. Conclusions: JWYA alleviates AD-like skin lesions and inhibited inflammation and skin itch. The effect of JWYA is attributed to blocking the JAK1/STAT3 and MAPK signaling pathways. We suggest that JWYA may be an alternative therapy for the treatment of AD.
MeSH term(s)	Animals ; Cytokines/metabolism ; Dermatitis, Atopic/chemically induced ; Dermatitis, Atopic/drug therapy ; Dermatitis, Atopic/metabolism ; Dinitrochlorobenzene ; Immunoglobulin E ; Interleukin-13/metabolism ; Interleukin-33/metabolism ; Interleukin-4/metabolism ; MAP Kinase Signaling System ; Mice ; Mice, Inbred BALB C ; Molecular Docking Simulation ; Plant Extracts/pharmacology ; Skin/pathology ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	Cytokines ; Dinitrochlorobenzene ; Interleukin-13 ; Interleukin-33 ; Plant Extracts ; Tumor Necrosis Factor-alpha ; Interleukin-4 (207137-56-2) ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2022-05-31
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115428
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Article: Jiu-Wei-Yong-An Formula suppresses JAK1/STAT3 and MAPK signaling alleviates atopic dermatitis-like skin lesions

Qinwufeng, Gu / Jiacheng, Lin / Xiaoling, Lu / Tingru, Chen / Yunyang, Wu / Yanlong, Yang

Journal of ethnopharmacology. 2022 Sept. 15, v. 295

2022

Abstract: Jiu-Wei-Yong-An (JWYA) formula is a traditional Chinese medicine (TCM) prescription used to treat ...

Abstract	Jiu-Wei-Yong-An (JWYA) formula is a traditional Chinese medicine (TCM) prescription used to treat atopic dermatitis (AD) in the clinic. JWYA is considered to have anti-inflammatory and antipruritic properties. However, the mechanism of JWYA remains unclear. Aim of the study: This study aimed to investigate the effect of JWYA on an experimental mouse AD model. Mice were sensitized with 2,4-dinitrochlorobenzene (DNCB) and intragastrically administered with JWYA for 14 days. The therapeutic effect was assessed using a grade four dermatitis score, skin moisture, thickness measurements, and a mouse behavior tests. H&E and toluidine blue staining were used to observe epidermal inflammatory thickening and mast cells in mouse skin lesions. Serum IgE levels and skin TNF-α and IL-4 levels were determined using ELISAs. The TNF-α, IL-1β, IL-4, IL-13, IL-31, IL-33, and IFN-γ mRNA expression levels in skin lesions were detected using qPCR. Network pharmacology analysis based on serum active components was performed to elucidate the mechanism, and the results were verified by Western blotting. Finally, we tested the binding affinity between the active ingredients of JWYA and JAK1 via molecular docking. JWYA improved the skin lesions of AD mice, relieved itching and reduced skin thickening. Additionally, JWYA decreased the serum IgE level and the levels of TNF-α, IL-1β, IL-4, IL-13, IL-31, IL-33, and IFN-γ in skin. Moreover, JWYA inhibited the activation of JAK1/STAT3 and MAPK (p38, ERK, and JNK) signaling. Molecular docking showed that kaempferol, luteolin, and forsythin have high affinity for JAK1. JWYA alleviates AD-like skin lesions and inhibited inflammation and skin itch. The effect of JWYA is attributed to blocking the JAK1/STAT3 and MAPK signaling pathways. We suggest that JWYA may be an alternative therapy for the treatment of AD.
Keywords	Oriental traditional medicine ; atopic dermatitis ; blood serum ; gene expression ; inflammation ; interleukin-13 ; interleukin-4 ; kaempferol ; luteolin ; mice ; models ; pharmacology ; therapeutics ; toluidine blue
Language	English
Dates of publication	2022-0915
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115428
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Article: [Epidemiological characteristics and environmental risk factors of hemorrhagic fever with renal syndrome in Wei River basin, China, 2005-2015].

Li, X / Liu, K / Gu, X / Yuan, X J / Shao, Z J

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

2018 Volume 39, Issue 9, Page(s) 1159–1164

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; Age Distribution ; China/epidemiology ; Endemic Diseases ; Hemorrhagic Fever with Renal Syndrome/epidemiology ; Humans ; Incidence ; Middle Aged ; Rain ; Risk Factors ; Rivers
Language	Chinese
Publishing date	2018-10-06
Publishing country	China
Document type	Journal Article
ZDB-ID	645026-x
ISSN	0254-6450
ISSN	0254-6450
DOI	10.3760/cma.j.issn.0254-6450.2018.09.004
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Article: Genetic Diversity of Nanmu (Phoebe zhennan S. Lee. et F. N. Wei) Breeding Population and Extraction of Core Collection Using nSSR, cpSSR and Phenotypic Markers

Zhu, Yan / An, Wenna / Peng, Jian / Li, Jinwu / Gu, Yunjie / Jiang, Bo / Chen, Lianghua / Zhu, Peng / Yang, Hanbo

Forests. 2022 Aug. 18, v. 13, no. 8

2022

Abstract: ... zhennan S. Lee. et F. N. Wei) is an extremely valuable tree species that can provide logs ...

Abstract	Genetic characterization is vital for tree germplasm utilization and conservation. Nanmu (Phoebe zhennan S. Lee. et F. N. Wei) is an extremely valuable tree species that can provide logs for many industrial products. This study aimed to assess the genetic diversity of a Nanmu breeding population using nine nSSR, five newly-developed cpSSR markers, and nine phenotypic traits, and extract a core collection. In general, the Na, Ne, and PIC for each nSSR/cpSSR were 2–37/2–3, 1.160–11.276/1.020–1.940, and 0.306–0.934/0.109–0.384, respectively. Fifteen chlorotype haplotypes were detected in 102 germplasms. The breeding population exhibited a relatively high level of genetic diversity for both nSSR (I = 1.768), cpSSR (I = 0.440, h = 0.286), and phenotypic traits (H′ = 1.98). Bayesian and cluster analysis clustered these germplasms into three groups. The germplasms revealed a high level of admixture between clusters, which indicated a relatively high level of gene exchange between germplasms. The F value (0.124) also showed a moderate genetic differentiation in the breeding population. A core collection consisting of 64 germplasms (62.7% of the whole germplasm) was extracted from phenotypic and molecular data, and the diversity parameters were not significantly different from those of the whole germplasm. Thereafter, a molecular identity was made up for each core germplasm. These results may contribute to germplasm management and conservation in the Nanmu breeding program, as well as genetics estimation and core collection extraction in other wood production rare species.
Keywords	Bayesian theory ; Phoebe zhennan ; cluster analysis ; genes ; genetic variation ; germplasm ; haplotypes ; phenotype ; rare species ; trees ; wood
Language	English
Dates of publication	2022-0818
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527081-3
ISSN	1999-4907
ISSN	1999-4907
DOI	10.3390/f13081320
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Article ; Online: Identification of differentially expressed genes in longissimus dorsi muscle between Wei and Yorkshire pigs using RNA sequencing.

Xu, Jingen / Wang, Chonglong / Jin, Erhui / Gu, Youfang / Li, Shenghe / Li, Qinggang

Genes & genomics

2017 Volume 40, Issue 4, Page(s) 413–421

Abstract: ... in the longissimus dorsi muscle of Wei pigs (a Chinese indigenous breed) and Yorkshire pigs (a Western lean-type ... breed) with different IMF content. For the Wei and Yorkshire pig libraries, over 57 and 64 million clean reads were ... and 394 down-regulated genes in Wei pigs compared with Yorkshire pigs. Gene Ontology analysis showed ...

Abstract	Intramuscular fat (IMF) content is an important trait closely related to meat quality, which is highly variable among pig breeds from diverse genetic backgrounds. High-throughput sequencing has become a powerful technique for analyzing the whole transcription profiles of organisms. In order to elucidate the molecular mechanism underlying porcine meat quality, we adopted RNA sequencing to detect transcriptome in the longissimus dorsi muscle of Wei pigs (a Chinese indigenous breed) and Yorkshire pigs (a Western lean-type breed) with different IMF content. For the Wei and Yorkshire pig libraries, over 57 and 64 million clean reads were generated by transcriptome sequencing, respectively. A total of 717 differentially expressed genes (DEGs) were identified in our study (false discovery rate < 0.05 and fold change > 2), with 323 up-regulated and 394 down-regulated genes in Wei pigs compared with Yorkshire pigs. Gene Ontology analysis showed that DEGs significantly related to skeletal muscle cell differentiation, phospholipid catabolic process, and extracellular matrix structural constituent. Pathway analysis revealed that DEGs were involved in fatty acid metabolism, steroid biosynthesis, glycerophospholipid metabolism, and protein digestion and absorption. Quantitative real time PCR confirmed the differential expression of 11 selected DEGs in both pig breeds. The results provide useful information to investigate the transcriptional profiling in skeletal muscle of different pig breeds with divergent phenotypes, and several DEGs can be taken as functional candidate genes related to lipid metabolism (ACSL1, FABP3, UCP3 and PDK4) and skeletal muscle development (ASB2, MSTN, ANKRD1 and ANKRD2).
MeSH term(s)	Adipose Tissue/metabolism ; Animal Husbandry/methods ; Animals ; Base Sequence/genetics ; Biomarkers ; Breeding ; Female ; Gene Expression Profiling/methods ; Gene Expression Regulation/genetics ; Gene Ontology ; High-Throughput Nucleotide Sequencing/methods ; Lipid Metabolism/genetics ; Meat ; Muscle Development/genetics ; Muscle, Skeletal/metabolism ; Phenotype ; Sequence Analysis, RNA ; Swine/genetics ; Transcriptome/genetics
Chemical Substances	Biomarkers
Language	English
Publishing date	2017-12-19
Publishing country	Korea (South)
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2504587-8
ISSN	2092-9293 ; 1976-9571
ISSN (online)	2092-9293
ISSN	1976-9571
DOI	10.1007/s13258-017-0643-3
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Article ; Online: The efficacy of the traditional Chinese medicine Jia Wei Niu Bang Zi granule combined with methotrexate in treating active rheumatoid arthritis: A multicenter, randomized, double-blinded controlled clinical trial.

Wang, Yi-Ru / Liu, Li / Wang, Xiao-Yun / Wang, Qiong / Yao, Min / Cui, Xue-Jun / Mao, Jian-Chun / Liu, Jian / Gu, Xiao-Hua / Chen, Yong-Li / Yu, Xiang / Shi, Qi / Liang, Qian-Qian / Wang, Yong-Jun

Medicine

2019 Volume 98, Issue 6, Page(s) e14424

Abstract: ... to benefit patients with RA by large. Jia Wei Niu Bang Zi granule (NBZG) consisting of RA-fighting Chinese ...

Abstract	Background: Rheumatoid arthritis (RA) is a chronic systemic and autoimmune inflammatory disease ending with the destruction of joints. Current therapies can relieve RA symptoms, but some also bring severe adverse events. Therefore, an effective and safe therapeutic strategy remains to be created to benefit patients with RA by large. Jia Wei Niu Bang Zi granule (NBZG) consisting of RA-fighting Chinese herbals has been used in Longhua Hospital in the last several decades. NBZG has potential therapeutic effect on RA, which should be evaluated by larger sample clinical trial. Methods: A multicenter, randomized, double-blind, placebo-controlled clinical trials will be conducted to determine the efficiency of NBZG in pain relief and joint protection. A total of 120 patients with active RA will be enrolled, and treated with NBZG or placebo for 12 weeks. The primary outcome measurements include rate of American College of Rheumatology (ACR) 50 at 12 weeks' treatment. The 2nd outcome measurements include rate change of ACR20, ACR70, the disease activity score (DAS) 28, 36-item Short-Form Health Survey Questionnaire, Health Assessment Questionnaire - Disability Index, score changes of Patient Assessment of Arthritis Pain, Patient Global Assessment of Arthritis, and the Athens insomnia scale at the same time points. Discussion: Although NBZG has shown efficacy in treating RA in Longhua Hospital for decades, the universality of this efficacy needs evaluated. The results of this trial will provide a convincing evidence about NBZG's efficacy in treating active RA in a large population. Trial registration: ClinicalTrials.gov ID: NCT03173040 (registered on May 30, 2017).
MeSH term(s)	Adolescent ; Adult ; Antirheumatic Agents/administration & dosage ; Arthritis, Rheumatoid/drug therapy ; Double-Blind Method ; Drug Therapy, Combination ; Drugs, Chinese Herbal/administration & dosage ; Female ; Humans ; Male ; Medicine, Chinese Traditional/methods ; Methotrexate/administration & dosage ; Middle Aged ; Randomized Controlled Trials as Topic ; Treatment Outcome ; Young Adult
Chemical Substances	Antirheumatic Agents ; Drugs, Chinese Herbal ; Methotrexate (YL5FZ2Y5U1)
Language	English
Publishing date	2019-02-15
Publishing country	United States
Document type	Clinical Trial Protocol ; Journal Article ; Multicenter Study
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000014424
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Article: Identification of differentially expressed genes in longissimus dorsi muscle between Wei and Yorkshire pigs using RNA sequencing

Xu, Jingen / Chonglong Wang / Erhui Jin / Youfang Gu / Shenghe Li / Qinggang Li

Genes & genomics. 2018 Apr., v. 40, no. 4

2018

Abstract	Intramuscular fat (IMF) content is an important trait closely related to meat quality, which is highly variable among pig breeds from diverse genetic backgrounds. High-throughput sequencing has become a powerful technique for analyzing the whole transcription profiles of organisms. In order to elucidate the molecular mechanism underlying porcine meat quality, we adopted RNA sequencing to detect transcriptome in the longissimus dorsi muscle of Wei pigs (a Chinese indigenous breed) and Yorkshire pigs (a Western lean-type breed) with different IMF content. For the Wei and Yorkshire pig libraries, over 57 and 64 million clean reads were generated by transcriptome sequencing, respectively. A total of 717 differentially expressed genes (DEGs) were identified in our study (false discovery rate < 0.05 and fold change > 2), with 323 up-regulated and 394 down-regulated genes in Wei pigs compared with Yorkshire pigs. Gene Ontology analysis showed that DEGs significantly related to skeletal muscle cell differentiation, phospholipid catabolic process, and extracellular matrix structural constituent. Pathway analysis revealed that DEGs were involved in fatty acid metabolism, steroid biosynthesis, glycerophospholipid metabolism, and protein digestion and absorption. Quantitative real time PCR confirmed the differential expression of 11 selected DEGs in both pig breeds. The results provide useful information to investigate the transcriptional profiling in skeletal muscle of different pig breeds with divergent phenotypes, and several DEGs can be taken as functional candidate genes related to lipid metabolism (ACSL1, FABP3, UCP3 and PDK4) and skeletal muscle development (ASB2, MSTN, ANKRD1 and ANKRD2).
Keywords	absorption ; biosynthesis ; cell differentiation ; digestion ; extracellular matrix ; fatty acid metabolism ; gene expression regulation ; gene ontology ; genes ; genetic background ; high-throughput nucleotide sequencing ; intramuscular fat ; longissimus muscle ; meat quality ; muscle development ; phenotype ; phospholipids ; pork ; quantitative polymerase chain reaction ; skeletal muscle ; swine ; swine breeds ; transcription (genetics) ; transcriptome ; transcriptomics
Language	English
Dates of publication	2018-04
Size	p. 413-421.
Publishing place	The Genetics Society of Korea
Document type	Article
ZDB-ID	2504587-8
ISSN	2092-9293 ; 1976-9571
ISSN (online)	2092-9293
ISSN	1976-9571
DOI	10.1007/s13258-017-0643-3
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Article: Changes in the long-term hydrological regimes and the impacts of human activities in the main Wei River, China

Zhang, Hongbo / Qiang Huang / Qiang Zhang / Lei Gu / Keyu Chen / Qijun Yu

Hydrological sciences journal. 2016 Apr. 25, v. 61, no. 6

2016

Abstract: ... of the Wei River shows remarkable variations which have caused many issues in the Wei River in recent decades ... runoff series at four hydrological stations on the main Wei River and quantifies features of their long ... the entire main stream of the Wei River and the more serious decrease emerging in measured flow should result ...

Abstract	Under the combined influence of climate changes and human activities, the hydrological regime of the Wei River shows remarkable variations which have caused many issues in the Wei River in recent decades, such as a lack of freshwater, water pollution, disastrous flooding and channel sedimentation. Hence, hydrological regime changes and potential human-induced impacts have been drawing increasing attention from local government and hydrologists. This study investigates hydrological regime changes in the natural and measured runoff series at four hydrological stations on the main Wei River and quantifies features of their long-term change by analysing their historical annual and seasonal runoff data using several approaches, i.e., continuous wavelet transform, cross-wavelet, wavelet coherence, trend-free pre-whitening Mann-Kendall test and detrended fluctuation analysis. By contrasting two different analysis results between natural and measured river runoff series, the impacts of human activities on the long-term hydrological regime were investigated via the changes of spatio-temporal distribution in dominant periods, the trends and long-range memory of river runoff. The results show : (a) that periodic properties of the streamflow changes are the result of climate, referring to precipitation changes in particular, while human activities play a minor role; (b) a significant decreasing trend can be observed in the natural streamflow series along the entire main stream of the Wei River and the more serious decrease emerging in measured flow should result from human-induced influences in recent decades; and (c) continuous decreasing streamflow in the Wei River will trigger serious shortages of freshwater in the future, which may challenge the sustainability and safety of water resources development in the river basin, and should be paid great attention before 2020. Editor Z.W. Kundzewicz; Associate editor K. Hamed
Keywords	anthropogenic activities ; climate ; climate change ; freshwater ; humans ; local government ; play activities ; rivers ; runoff ; stream flow ; water pollution ; watersheds ; wavelet ; China
Language	English
Dates of publication	2016-0425
Size	p. 1054-1068.
Publishing place	Taylor & Francis
Document type	Article
ISSN	2150-3435
DOI	10.1080/02626667.2015.1027708
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Article: Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice.

Zhao, Ai-Guang / Li, Ting / You, Sheng-Fu / Zhao, Hai-Lei / Gu, Ying / Tang, Lai-Di / Yang, Jin-Kun

World journal of gastroenterology

2008 Volume 14, Issue 5, Page(s) 693–700

Abstract: Aim: To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang ...

Abstract	Aim: To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang An (WCA) in human gastric cancer cell line SGC-7901. Methods: A human gastric adenocarcinoma cell line SGC-7901 grafted onto nude mice was used as the animal model. The mice were randomly divided into 3 groups, one control and the two representing experimental conditions. Animals in the two experimental groups received either WCA over a 34-d period or 5-fluorouracil (5-FU) over 6-d period starting at 8th d after grafting. Control animals received saline on an identical schedule. Animals were killed 41 d after being grafted. The expression profiles in paired WCA treated gastric cancer samples and the N.S. control samples were studied by using a cDNA array representing 14181 cDNA clusters. The alterations in gene expression levels were confirmed by Real-time Quantitative polymerase chain reaction (qPCR). Results: When compared with controls, the average tumor inhibitory rate in WCA group was 44.32% +/- 5.67% and 5-FU 47.04% +/- 11.33% (P < 0.01, respectively). The average labeling index (LI) for PCNA in WCA group and 5-FU group was significantly decreased compared with the control group. Apoptotic index (AI) was significantly increased to 9.72% +/- 4.51% using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling (TUNEL) method in WCA group compared with the controls 2.45% +/- 1.37%. 5-FU group was also found to have a significantly increased AI compared with the controls. The expression of cleaved Caspase-3 in WCA group and 5-FU group was significantly increased compared with the control group respectively. There were 45 different expressed sequence tags (ESTs) among the control sample pool and WCA sample pool. There were 24 ESTs up-regulated in WCA samples and 21 ESTs down-regulated. By using qPCR, the expression level of Stat3, rap2 interacting protein x (RIPX), regulator of differentiation 1 (ROD1) and Bcl-2 was lower in WCA group than that in control group respectively. By using SP immunohistochemical method the expression of Phospho-Stat3 (Tyr705) and Bcl-2 in WCA group and 5-FU group was significantly decreased compared with the control group respectively. Conclusion: WCA could inhibit gastric cancer cell SGC-7901 growth in vivo. WCA could induce gastric cancer cell apoptosis and suppress proliferation. Its mechanisms might be involved in the down-regulation of Stat3, RIPX, ROD1 and Bcl-2 gene.
MeSH term(s)	Adenocarcinoma/drug therapy ; Adenocarcinoma/genetics ; Adenocarcinoma/pathology ; Animals ; Atractylodes ; Cell Line, Tumor ; Drugs, Chinese Herbal/pharmacology ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Polyporales ; Prunella ; Stomach Neoplasms/drug therapy ; Stomach Neoplasms/genetics ; Stomach Neoplasms/pathology ; Xenograft Model Antitumor Assays
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2008-01-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.14.693
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Article: Simultaneous determination of 12 chemical constituents in the traditional Chinese Medicinal Prescription Xiao-Yao-San-Jia-Wei by HPLC coupled with photodiode array detection.

Zhang, Hongmin / Chen, Shiwei / Qin, Feng / Huang, Xi / Ren, Ping / Gu, Xinqi

Journal of pharmaceutical and biomedical analysis

2008 Volume 48, Issue 5, Page(s) 1462–1466

Abstract: ... of 12 components in Xiao-Yao-San-Jia-Wei (XYSJW): geniposide, puerarin, paeoniflorin, ferulic acid ...

Abstract	An HPLC-photodiode array (PDA) detection method was established for the simultaneous determination of 12 components in Xiao-Yao-San-Jia-Wei (XYSJW): geniposide, puerarin, paeoniflorin, ferulic acid, liquiritin, hesperidin, naringin, paeonol, daidzein, glycyrrhizic acid, honokiol, and magnolol. These were separated in less than 70 min using a Waters Symmetry Shield RP 18 column with gradient elution using (A) acetonitrile, (B) water, and (C) acetic acid at a flow rate of 1 ml/min, and with a PDA detector. All calibration curves showed good linear regression (r(2)>0.9992) within the test ranges. The method was validated for specificity, accuracy, precision, and limits of detection. The proposed method enables in a single run the simultaneous identification and determination for quality control of 12 multi-structural components of XYSJW forming the basis of its therapeutic effect.
MeSH term(s)	Acetophenones/chemistry ; Benzoates/chemistry ; Biphenyl Compounds/chemistry ; Bridged-Ring Compounds/chemistry ; Coumaric Acids/chemistry ; Drugs, Chinese Herbal ; Flavanones/chemistry ; Glucosides/chemistry ; Glycyrrhizic Acid/chemistry ; Hesperidin/chemistry ; Iridoids/chemistry ; Isoflavones/chemistry ; Lignans/chemistry ; Medicine, Chinese Traditional ; Molecular Structure ; Monoterpenes ; Quality Control ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity
Chemical Substances	Acetophenones ; Benzoates ; Biphenyl Compounds ; Bridged-Ring Compounds ; Coumaric Acids ; Drugs, Chinese Herbal ; Flavanones ; Glucosides ; Iridoids ; Isoflavones ; Lignans ; Monoterpenes ; xiaoyao ; magnolol (001E35HGVF) ; honokiol (11513CCO0N) ; geniposide (145295QLXY) ; peoniflorin (21AIQ4EV64) ; paeonol (3R834EPI82) ; daidzein (6287WC5J2L) ; Glycyrrhizic Acid (6FO62043WK) ; ferulic acid (AVM951ZWST) ; Hesperidin (E750O06Y6O) ; naringin (N7TD9J649B) ; liquiritin (T0O79T74CD) ; puerarin (Z9W8997416)
Language	English
Publishing date	2008-12-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2008.09.023
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In stock of ZB MED Cologne/Königswinter

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Order via subito

Inter-library loan at ZB MED

Full text online

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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Cologne/Königswinter

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