Article ; Online: R-Spondin1 enhances wnt signaling and decreases weight loss in short bowel syndrome zebrafish.
Biochemistry and biophysics reports
2021 Volume 25, Page(s) 100874
Abstract: Background: R-spondins, including R-spondin 1 (RSPO1), are a family of Wnt ligands that help ...
Abstract | Background: R-spondins, including R-spondin 1 (RSPO1), are a family of Wnt ligands that help to activate the canonical Wnt/β-catenin pathway, which is critical for intestinal epithelial cell proliferation and maintenance of intestinal stem cells. This proliferation underpins the epithelial expansion, or intestinal adaptation (IA), that occurs following massive bowel resection and short bowel syndrome (SBS). The purpose of this study was to identify if recombinant human RSPO1 (rhRSPO1) could be serially administered to SBS zebrafish to enhance cellular proliferation and IA. Methods: Adult male zebrafish were assigned to four groups: sham + PBS, SBS + PBS, sham + rhRSPO1, and SBS + rhRSPO1. Sham fish had a laparotomy alone. SBS fish had a laparotomy with distal intestinal ligation and creation of a proximal stoma. Fish were weighed at initial surgery and then weekly. rhRSPO1 was administered post-operatively following either a one- or two-week dosing schedule with either 3 or 5 intraperitoneal injections, respectively. Fish were harvested at 7 or 14 days with intestinal segments collected for analysis. Results: Repeated intraperitoneal injection of rhRSPO1 was feasible and well tolerated. At 7 days, intestinal epithelial proliferation was increased by rhRSPO1. At 14 days, SBS + rhRSPO1 fish lost significantly less weight than SBS + PBS fish. Measurements of intestinal surface area were not increased by rhRSPO1 administration but immunofluorescent staining for β-catenin and gene expression for Conclusions: Intraperitoneal injection of rhRSPO1 decreased weight loss in SBS zebrafish with increased β-catenin + cells and |
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Language | English |
Publishing date | 2021-01-04 |
Publishing country | Netherlands |
Document type | Journal Article |
ZDB-ID | 2831046-9 |
ISSN | 2405-5808 ; 2405-5808 |
ISSN (online) | 2405-5808 |
ISSN | 2405-5808 |
DOI | 10.1016/j.bbrep.2020.100874 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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