Article ; Online: GTP-stimulated membrane fission by the N-BAR protein AMPH-1.
2022 Volume 24, Issue 1, Page(s) 34–47
Abstract: Membrane-enclosed transport carriers sort biological molecules between stations in the cell in a dynamic process that is fundamental to the physiology of eukaryotic organisms. While much is known about the formation and release of carriers from specific ... ...
Abstract | Membrane-enclosed transport carriers sort biological molecules between stations in the cell in a dynamic process that is fundamental to the physiology of eukaryotic organisms. While much is known about the formation and release of carriers from specific intracellular membranes, the mechanism of carrier formation from the recycling endosome, a compartment central to cellular signaling, remains to be resolved. In Caenorhabditis elegans, formation of transport carriers from the recycling endosome requires the dynamin-like, Eps15-homology domain (EHD) protein, RME-1, functioning with the Bin/Amphiphysin/Rvs (N-BAR) domain protein, AMPH-1. Here we show, using a free-solution single-particle technique known as burst analysis spectroscopy (BAS), that AMPH-1 alone creates small, tubular-vesicular products from large, unilamellar vesicles by membrane fission. Membrane fission requires the amphipathic H0 helix of AMPH-1 and is slowed in the presence of RME-1. Unexpectedly, AMPH-1-induced membrane fission is stimulated in the presence of GTP. Furthermore, the GTP-stimulated membrane fission activity seen for AMPH-1 is recapitulated by the heterodimeric N-BAR amphiphysin protein from yeast, Rvs161/167p, strongly suggesting that GTP-stimulated membrane fission is a general property of this important class of N-BAR proteins. |
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MeSH term(s) | Animals ; Cell Membrane/metabolism ; Endocytosis/physiology ; Endosomes/metabolism ; Intracellular Membranes ; Caenorhabditis elegans ; Guanosine Triphosphate/metabolism |
Chemical Substances | Guanosine Triphosphate (86-01-1) |
Language | English |
Publishing date | 2022-12-13 |
Publishing country | England |
Document type | Journal Article ; Research Support, N.I.H., Extramural |
ZDB-ID | 1483852-7 |
ISSN | 1600-0854 ; 1398-9219 |
ISSN (online) | 1600-0854 |
ISSN | 1398-9219 |
DOI | 10.1111/tra.12875 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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