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Article: LncRNAs signatures associated with cuproptosis predict the prognosis of endometrial cancer.

Qi, Shiyuan / Feng, Huiyan / Li, Xiaomao

2023 Volume 14, Page(s) 1120089

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2023-04-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2023.1120089
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Article ; Online: Intracellular metal enhanced fluorescence utilizing gold nanoparticles embedded in hydrogel droplets for sensitive protein detection in cells

David Sebben / Huiyan Li

Talanta Open, Vol 8, Iss , Pp 100265- (2023)

2023

Abstract: Cellular protein detection is necessary for monitoring various biological pathways associated with complex diseases such as cancer. Detecting intracellular proteins is more challenging than cell surface protein detection because most antibodies cannot ... ...

Abstract	Cellular protein detection is necessary for monitoring various biological pathways associated with complex diseases such as cancer. Detecting intracellular proteins is more challenging than cell surface protein detection because most antibodies cannot freely cross the cellular membrane. Cellular membrane permeabilization is thus required to facilitate the antibody diffusion and achieve adequate detection when combined with various conventional protein detection methods (e.g., flow cytometry and immunocytochemistry). However, these methods come with sensitivity challenges when a large number of cells are unavailable. Therefore, for early disease diagnostics and prognosis, novel methods are needed to achieve higher sensitivity to track disease-related proteins from small amounts of cells at early stages of disease progression. Here we applied a novel biosensing platform that co-entraps cells and gold nanoparticles in 3D hydrogel droplets to achieve sensitive fluorescence-based detection and quantification of intracellular proteins in intact cells. Importantly, intracellular metal enhanced fluorescence (iMEF) was achieved using gold nanoparticles via cell permeabilization. The intracellular gold-nanoparticles, together with the high cell loading capacity of the hydrogel droplets, enhanced the fluorescence signals and in turn improved assay sensitivity compared to conventional methods. After assay optimization, we demonstrated the detection of Heat Shock Protein 70, a common cancer marker, with as little as 250 cells. This technology was also able to distinguish between cancerous and non-cancerous samples when mimicking a clinical fine needle aspiration procedure. Compared to conventional assays measuring the same protein, the assay sensitivity was improved by two to three orders of magnitude, allowing for highly sensitive intracellular protein detection from minimal amounts of cells.
Keywords	Intracellular metal enhanced fluorescence (iMEF) ; Gold nanoparticles (AuNPs) ; Alginate hydrogel droplets ; Intracellular protein detection ; Sensitivity ; Analytical chemistry ; QD71-142
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: Management of a Peristomal Abscess in a Patient With an Ileostomy: A Case Study.

Wei, Huiyan / Qiu, Jin / Li, Aini / Hu, Hongyang

Journal of wound, ostomy, and continence nursing : official publication of The Wound, Ostomy and Continence Nurses Society

2024 Volume 51, Issue 1, Page(s) 74–77

Abstract: Background: Peristomal abscess (PA) is an uncommon but challenging peristomal skin complication. The initial treatment of the PA usually includes incision and drainage of the abscess, resulting in a peristomal wound. The presence of the wound makes it ... ...

Abstract	Background: Peristomal abscess (PA) is an uncommon but challenging peristomal skin complication. The initial treatment of the PA usually includes incision and drainage of the abscess, resulting in a peristomal wound. The presence of the wound makes it difficult to maintain a seal between the ostomy skin barrier and the peristomal skin resulting in frequent removal and application of the skin barrier to prevent leakage and allow for daily wound care. Case: Ms T was a 52-year-old woman with an ileostomy resulting from a prior left hemicolectomy for colon cancer who developed a PA. Treatment of the PA was implemented, along with a modified 2-piece skin barrier that allowed access to the peristomal wound for daily dressing changes while maintaining a seal around the ostomy. Conclusion: The modified 2-piece skin barrier technique proved a successful treatment for the management of the PA without frequent changes of the ostomy pouching system.
MeSH term(s)	Female ; Humans ; Middle Aged ; Ileostomy/adverse effects ; Ileostomy/methods ; Abscess/therapy ; Abscess/complications ; Ostomy/adverse effects ; Skin Diseases/etiology ; Skin ; Skin Care
Language	English
Publishing date	2024-01-12
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1188388-1
ISSN	1528-3976 ; 1071-5754
ISSN (online)	1528-3976
ISSN	1071-5754
DOI	10.1097/WON.0000000000001047
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Article ; Online: Identification of potential extracellular vesicle protein markers altered in osteosarcoma from public databases.

Zhang, Jinhe / Li, Huiyan

Proteomics. Clinical applications

2023 Volume 17, Issue 4, Page(s) e2200084

Abstract: Purpose: Extracellular vesicles (EVs) have become promising biomarkers for cancer management. Particularly, the molecular cargo such as proteins carried by EVs are similar to their cells of origin, providing important information that can be used for ... ...

Abstract	Purpose: Extracellular vesicles (EVs) have become promising biomarkers for cancer management. Particularly, the molecular cargo such as proteins carried by EVs are similar to their cells of origin, providing important information that can be used for cancer diagnostics, prognosis, and treatment monitoring. However, to date, molecular analysis on EVs is still challenging, limited by the availability of efficient analytical technologies, largely due to the small size of EVs. In this work, we developed a computational workflow for in silico identification of potential EV protein markers from genomic and proteomic databases, and applied it for the discovery of osteosarcoma (OS) EV protein markers. Experimental design: Both mRNA and protein data were computed and compared from publicly accessible databases, and top markers with high differential expression levels were selected. Results: Thirty nine markers were identified overexpressed and seven found to be downregulated. These identified markers have been found to be associated with OS on different aspects in literature, demonstrating the usability of this workflow. Conclusions and clinical relevance: This work provides a list of potential EV protein markers that are either overexpressed or downregulated in OS for further experimental validation for improved clinical management of OS.
MeSH term(s)	Humans ; Proteomics ; Osteosarcoma/genetics ; Osteosarcoma/metabolism ; Biomarkers/metabolism ; Extracellular Vesicles/genetics ; Extracellular Vesicles/metabolism ; Bone Neoplasms/genetics ; Bone Neoplasms/metabolism
Chemical Substances	Biomarkers
Language	English
Publishing date	2023-01-03
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2261788-7
ISSN	1862-8354 ; 1862-8346
ISSN (online)	1862-8354
ISSN	1862-8346
DOI	10.1002/prca.202200084
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Article ; Online: Comparison of blocking reagents for antibody microarray-based immunoassays on glass and paper membrane substrates.

Ströhle, Gisela / Li, Huiyan

Analytical and bioanalytical chemistry

2023 Volume 415, Issue 10, Page(s) 1967–1977

Abstract: Background noise due to nonspecific binding of biomolecules on the assay substrates is one of the most common challenges that limits the sensitivity of microarray-based immunoassays. Background signal intensity usually increases when complex biological ... ...

Abstract	Background noise due to nonspecific binding of biomolecules on the assay substrates is one of the most common challenges that limits the sensitivity of microarray-based immunoassays. Background signal intensity usually increases when complex biological fluids are used because they have a combination of molecules and vesicles that can adsorb onto substrate surfaces. Blocking strategies coupled with surface chemistries can reduce such nonspecific binding and improve assay sensitivity. In this paper, we conducted a systematic optimization of blocking strategies on a variety of commonly used substrates for protein measurement in complex biofluids. Four blocking strategies (BSA, non-fat milk, PEG, and a protein-free solution) coupled with four surface chemistries (3-glycidoxypropyltrimethoxysilane (GPS), poly-L-lysine (PLL), aminoalkylsilane (AAS), and nitrocellulose (NC)) were studied for their effect on background, microspot, and net signal intensities. We have also explored the effect that these blocking strategies have when proteins in complex samples (plasma, serum, cell culture media, and EV lysate) are measured. Irregular spot morphology could affect signal extraction using automated software. We found that the microspots with the best morphology were the ones printed on GPS glass surfaces for all immunoassays. On NC membrane, the protein-based blocking strategies yielded the highest net fluorescent intensity with the antigen contained in PBS, plasma, serum, and serum-free cell culture media. Differently, with EV lysate samples, Pierce™ protein-free blocker yielded the best net signal intensity on both GPS and NC surfaces. The choice of blocking strategies highly depends on the substrate. Moreover, the findings discovered in this study are not limited to microarray-based immunoassays but can provide insights for other assay formats.
MeSH term(s)	Indicators and Reagents ; Antibodies ; Microarray Analysis ; Proteins/chemistry ; Immunoassay ; Surface Properties
Chemical Substances	Indicators and Reagents ; Antibodies ; Proteins
Language	English
Publishing date	2023-02-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-023-04614-w
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Article ; Online: Advances in three dimensional metal enhanced fluorescence based biosensors using metal nanomaterial and nano-patterned surfaces.

Goodrum, Rebecca / Li, Huiyan

Biotechnology journal

2023 Volume 19, Issue 1, Page(s) e2300519

Abstract: Metal enhanced fluorescence (MEF) is a phenomenon that increases fluorescence signal through placement of metal near a fluorophore. For biosensing applications, MEF-based biosensors are becoming increasingly popular as it enables highly sensitive ... ...

Abstract	Metal enhanced fluorescence (MEF) is a phenomenon that increases fluorescence signal through placement of metal near a fluorophore. For biosensing applications, MEF-based biosensors are becoming increasingly popular as it enables highly sensitive detection of molecules, important for early diagnosis. The structure and size of the metal influence the optical properties through enhancing the fluorophore photostability and light absorption and emission. In recent years, many metal nanostructures have been fabricated and examined for their effectiveness in developing MEF-based biosensors. This review focuses on the latest applications of three-dimensional nanostructures and nano-patterned surfaces used to develop and improve fluorescence sensing via MEF. Current reviews mostly discussed the applications of two dimensional MEF and metal-nanoparticles-based MEF with a focus on fabrication of nanoparticles and metal substrates. In this article, we focused more on the effect of the metal nanostructure and size on MEF and then provided an in-depth summary of the performance of the state-of-the-art three dimensional MEF-based biosensors. While more work is needed to demonstrate applicability for complex samples, it is evident that with the use of metal nanoparticles and three dimensional nano-patterns, the assay sensitivity of fluorescence-based detection can be greatly improved, making it suitable for use in early disease diagnostics.
MeSH term(s)	Spectrometry, Fluorescence/methods ; Metals/chemistry ; Fluorescent Dyes/chemistry ; Nanostructures/chemistry ; Biosensing Techniques/methods ; Metal Nanoparticles/chemistry
Chemical Substances	Metals ; Fluorescent Dyes
Language	English
Publishing date	2023-12-05
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2221885-3
ISSN	1860-7314 ; 1860-6768
ISSN (online)	1860-7314
ISSN	1860-6768
DOI	10.1002/biot.202300519
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Article ; Online: Mycobacterium haemophilum infection in immunocompetent adults: a literature review and case report.

Sun, Yuan / Li, Huiyan

International journal of dermatology

2023 Volume 63, Issue 2, Page(s) 169–176

Abstract: Background: Mycobacterium haemophilum has been increasingly found in severely immunocompromised patients but is scarcely reported in immunocompetent adults.: Methods: We systematically reviewed previous literature to identify studies on infection in ... ...

Abstract	Background: Mycobacterium haemophilum has been increasingly found in severely immunocompromised patients but is scarcely reported in immunocompetent adults. Methods: We systematically reviewed previous literature to identify studies on infection in immunocompetent adults. Articles reporting at least one case of M. haemophilum infection were included. We excluded articles involving patients who had immunosuppression-related diseases and routinely used glucocorticoids or immunosuppressants. We also reported a case of a young immunocompetent woman infected by M. haemophilum along the eyebrows, which was probably due to the use of an eyebrow pencil retrieved from a sink drain. Results: Twelve qualifying articles reporting M. haemophilum infection in immunocompetent adults were identified. Among them, most cases report skin lesions along the eyebrows, and the remaining had cervicofacial lymphadenitis, lesions on the arm or fingers, inflammation in the eyeballs, or ulceration in the perineal region. Most cases were caused by tattoos, make-up, injury, or surgical operation. For diagnosis, specialized tissue culture sensitivity was roughly 75%, and polymerase chain reaction (PCR) test sensitivity was approximately 89%. Triple antibiotic therapy for 3 to 24 months, or surgical excision was effective in controlling infection. Conclusion: M. haemophilum infection should be considered if routine antibacterial and glucocorticoid treatments are ineffective against the disease, even in healthy adults. To definitively diagnose this infection, conditioned tissue culture or PCR testing is required. Treatment usually involves a combination of multiple antibiotics and, if necessary, surgical removal of infected tissue.
MeSH term(s)	Adult ; Female ; Humans ; Mycobacterium haemophilum ; Anti-Bacterial Agents/therapeutic use ; Lymphadenitis/diagnosis ; Mycobacterium Infections/diagnosis ; Mycobacterium Infections/drug therapy ; Mycobacterium Infections/microbiology ; Inflammation
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-12-06
Publishing country	England
Document type	Systematic Review ; Case Reports ; Journal Article ; Review
ZDB-ID	412254-9
ISSN	1365-4632 ; 0011-9059 ; 1461-1244
ISSN (online)	1365-4632
ISSN	0011-9059 ; 1461-1244
DOI	10.1111/ijd.16874
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Article ; Online: Investigating genetic links between blood metabolites and preeclampsia.

Lin, Qiannan / Li, Siyu / Wang, Huiyan / Zhou, Wenbo

BMC women's health

2024 Volume 24, Issue 1, Page(s) 223

Abstract: Background: Observational studies have revealed that metabolic disorders are closely related to the development of preeclampsia (PE). However, there is still a research gap on the causal role of metabolites in promoting or preventing PE. We aimed to ... ...

Abstract	Background: Observational studies have revealed that metabolic disorders are closely related to the development of preeclampsia (PE). However, there is still a research gap on the causal role of metabolites in promoting or preventing PE. We aimed to systematically explore the causal association between circulating metabolites and PE. Methods: Single nucleotide polymorphisms (SNPs) from genome-wide association study (GWAS) of 486 blood metabolites (7,824 participants) were extracted as instrumental variables (P < 1 × 10 Results: The instrumental variables for the metabolites ranged from 3 to 493. Primary analysis revealed a total of 12 known (e.g., phenol sulfate, citrulline, lactate and gamma-glutamylglutamine) and 11 unknown metabolites were associated with PE. Heterogeneity and pleiotropy tests verified the robustness of the MR results. Validation with another metabolite GWAS dataset revealed consistency trends in 6 of the known metabolites with preliminary analysis, particularly the finding that genetic susceptibility to low levels of arachidonate (20:4n6) and citrulline were risk factors for PE. The pathway analysis revealed glycolysis/gluconeogenesis and arginine biosynthesis involved in the pathogenesis of PE. Conclusions: This study identifies a causal relationship between some circulating metabolites and PE. Our study presented new perspectives on the pathogenesis of PE by integrating metabolomics with genomics, which opens up avenues for more accurate understanding and management of the disease, providing new potential candidate metabolic molecular markers for the prevention, diagnosis and treatment of PE. Considering the limitations of MR studies, further research is needed to confirm the causality and underlying mechanisms of these findings.
MeSH term(s)	Female ; Pregnancy ; Humans ; Citrulline ; Genome-Wide Association Study ; Pre-Eclampsia/genetics ; Genetic Predisposition to Disease ; Lactic Acid
Chemical Substances	Citrulline (29VT07BGDA) ; Lactic Acid (33X04XA5AT)
Language	English
Publishing date	2024-04-05
Publishing country	England
Document type	Meta-Analysis ; Journal Article
ZDB-ID	2050444-5
ISSN	1472-6874 ; 1472-6874
ISSN (online)	1472-6874
ISSN	1472-6874
DOI	10.1186/s12905-024-03000-7
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Article ; Online: Progressive sarcopenia and myosteatosis predict prognosis of advanced HCC patients treated with immune checkpoint inhibitors.

Liu, Mengchen / Jin, Qianna / Wang, Huiyan / Li, Yunqiao

Frontiers in immunology

2024 Volume 15, Page(s) 1396927

Abstract: Background: Immunotherapy stands as a pivotal modality in the therapeutic landscape for the treatment of advanced hepatocellular carcinoma, yet responses vary among patients. This study delves into the potential impact of sarcopenia, myosteatosis and ... ...

Abstract	Background: Immunotherapy stands as a pivotal modality in the therapeutic landscape for the treatment of advanced hepatocellular carcinoma, yet responses vary among patients. This study delves into the potential impact of sarcopenia, myosteatosis and adiposity indicators, as well as their changes during immunotherapy, on treatment response and prognosis in patients with advanced hepatocellular carcinoma treated with immune checkpoint inhibitors. Methods: In this retrospective analysis, 116 patients with advanced hepatocellular carcinoma receiving immune checkpoint inhibitors were recruited. Skeletal muscle, intramuscular, subcutaneous, and visceral adipose tissue were assessed by computed tomography at the level of the third lumbar vertebrae before and after 3 months of treatment. Sarcopenia and myosteatosis were evaluated by skeletal muscle index and mean muscle density using predefined threshold values. Patients were stratified based on specific baseline values or median values, along with alterations observed during the treatment course. Overall survival (OS) and progression-free survival (PFS) were compared using the log-rank test and a multifactorial Cox proportional risk model. Results: A total of 116 patients were recruited and divided into two cohorts, 81 patients for the training set and 35 patients for the validating set. In the overall cohort, progressive sarcopenia ( Conclusion: Progressive sarcopenia and myosteatosis are predictors of poor clinical outcomes in patients with advanced hepatocellular carcinoma receiving immune checkpoint inhibitors, and high baseline visceral adiposity is associated with a poorer survival.
MeSH term(s)	Humans ; Sarcopenia/etiology ; Sarcopenia/diagnosis ; Male ; Female ; Immune Checkpoint Inhibitors/therapeutic use ; Immune Checkpoint Inhibitors/adverse effects ; Middle Aged ; Liver Neoplasms/mortality ; Liver Neoplasms/drug therapy ; Carcinoma, Hepatocellular/mortality ; Carcinoma, Hepatocellular/drug therapy ; Retrospective Studies ; Aged ; Prognosis ; Adult ; Muscle, Skeletal/pathology ; Adiposity
Chemical Substances	Immune Checkpoint Inhibitors
Language	English
Publishing date	2024-04-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1396927
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Article ; Online: Short-Term Efficacy of Er:YAG Laser and Nd:YAG Laser Combined Therapy on Periodontitis.

Gong, Huiyan / Li, Meihua

Photobiomodulation, photomedicine, and laser surgery

2022 Volume 40, Issue 8, Page(s) 580–588

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Aluminum ; Follow-Up Studies ; Humans ; Lasers, Solid-State/therapeutic use ; Periodontal Pocket ; Periodontitis/radiotherapy ; Yttrium
Chemical Substances	Yttrium (58784XQC3Y) ; Aluminum (CPD4NFA903)
Language	English
Publishing date	2022-08-12
Publishing country	United States
Document type	Journal Article
ISSN	2578-5478
ISSN (online)	2578-5478
DOI	10.1089/photob.2022.0045
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