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Article: Quantitative and qualitative determination of dimethyl phthalate and N, N-diethyl-m-toluamide in repellents commercial formulations by high performance thin layer chromatography.

Khoobdel, Mehdi / Jonaidi, Nematollah / Sharif, Babak

Pakistan journal of biological sciences : PJBS

2008 Volume 10, Issue 20, Page(s) 3678–3682

Abstract: In this study HPTLC was used for simultaneous quantitative and qualitative determination of N, N ...

Abstract	In this study HPTLC was used for simultaneous quantitative and qualitative determination of N, N-diethyl meta toluamide (DEET) and dimethyl phthalate (DMP), which are the main elements and active ingredients in current chemical repellents. Some defined amounts of commercial form of 3 repellents included trench pomade, stick insect repellent (SIR), which is containing 33% of DEET and DMP60 (dimethyl phthalate 60%) dissolved in ethyl acetate solvent, separately. The method employed TLC aluminum plate precoated with silica gel plates (SiO4) 60F245 as the stationary phase. The solvent system consisted of benzene-diethyl etherhexane (5:3:2, v/v/v) as mobile phase. The multiple level method used for spotting. Densitometric analysis of repellents was carried out using TLC scanner 3 and CATS4 software in the absorption/reflection mode at 230 nm. According to the results, the type and amount of active ingredients in DMP60 lotion was 61.8 g (SE = +/-1.6) per 100 cc and in SIR, 31.3 g (SE = +/-0.8) diethyl meta toluamide per 100 g of repellents raw materials. Also the active ingredients in trench pomade were determined as a combination of DMP and DEET by rates of 5.5 g (SE = +/-0.2) and 25 g (SE = +/-l) per 100 g repellents commercial formulations, respectively. In this study, the value of Rf for DMP and DEET was calculated 0.71 +/- 0.2 and 0.32 +/- 0.2, respectively. HPTLC is a suitable method to quantitatively and quantitatively determine repellents which have DMP and DEET active ingredients. Since most of commercial chemical repellents have this active ingredient, adjusting and setting HPTLC up can be important.
MeSH term(s)	Animals ; Chromatography, Thin Layer/methods ; DEET/analysis ; Humans ; Insect Repellents/chemistry ; Phthalic Acids/analysis
Chemical Substances	Insect Repellents ; Phthalic Acids ; dimethyl phthalate (08X7F5UDJM) ; DEET (134-62-3)
Language	English
Publishing date	2008-12-17
Publishing country	Pakistan
Document type	Journal Article
ISSN	1028-8880
ISSN	1028-8880
DOI	10.3923/pjbs.2007.3678.3682
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Article ; Online: Quantitative and Qualitative Determination of Dimethyl Phthalate and N, N-Diethyl-m-Toluamide in Repellents Commercial Formulations by High Performance Thin Layer Chromatography

Mehdi Khoobdel / Nematollah Jonaidi / Babak Sharif

Pakistan Journal of Biological Sciences, Vol 10, Iss 20, Pp 3678-

2007 Volume 3682

Abstract: In this study HPTLC was used for simultaneous quantitative and qualitative determination of N, N ...

Abstract	In this study HPTLC was used for simultaneous quantitative and qualitative determination of N, N-diethyl meta toluamide (DEET) and dimethyl phthalate (DMP), which are the main elements and active ingredients in current chemical repellents. Some defined amounts of commercial form of 3 repellents included trench pomade, stick insect repellent (SIR), which is containing 33% of DEET and DMP60 (dimethyl phthalate 60%) dissolved in ethyl acetate solvent, separately. The method employed TLC aluminum plate precoated with silica gel plates (SiO 4 ) 60F 245 as the stationary phase. The solvent system consisted of benzene-diethyl ether-hexane (5:3:2, v/v/v) as mobile phase. The multiple level method used for spotting. Densitometric analysis of repellents was carried out using TLC scanner 3 and CATS4 software in the absorption/reflection mode at 230 nm. According to the results, the type and amount of active ingredients in DMP60 lotion was 61.8 g (SE = ±1.6) per 100 cc and in SIR, 31.3 g (SE = ±0.8) diethyl meta toluamide per 100 g of repellents raw materials. Also the active ingredients in trench pomade were determined as a combination of DMP and DEET by rates of 5.5 g (SE = ±0.2) and 25 g (SE = ±1) per 100 g repellents commercial formulations, respectively. In this study, the value of R f for DMP and DEET was calculated 0.71±0.2 and 0.32±0.2, respectively. HPTLC is a suitable method to quantitatively and quantitatively determine repellents which have DMP and DEET active ingredients. Since most of commercial chemical repellents have this active ingredient, adjusting and setting HPTLC up can be important.
Keywords	Thin layer chromatography ; repellents ; dimethyl phthalate ; diethyl tuloamide ; Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Subject code	540
Language	English
Publishing date	2007-01-01T00:00:00Z
Publisher	Asian Network for Scientific Information
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Affinities of muscarinic drugs for [3H]N-methylscopolamine (NMS) and [3H]oxotremorine (OXO) binding to a mixture of M1-M4 muscarinic receptors: use of NMS/OXO-M ratios to group compounds into potential agonist, partial agonist, and antagonist classes.

Sharif, N A / Williams, G W / DeSantis, L M

Neurochemical research

1995 Volume 20, Issue 6, Page(s) 669–674

Abstract: The relative affinities of various muscarinic drugs in the antagonist ([3H]N-methyl scopolamine ... 3H]NMS)) and agonist ([3H]Oxotremorine-m ([3H]OXO-M)) binding assays using a mixture of tissues ... n = 3) and to a high density Bmax = 11.8 +/- 0.025 nmol/g wet weight) of muscarinic receptors. [3H ...

Abstract	The relative affinities of various muscarinic drugs in the antagonist ([3H]N-methyl scopolamine ([3H]NMS)) and agonist ([3H]Oxotremorine-m ([3H]OXO-M)) binding assays using a mixture of tissues containing M1-M4 receptor subtypes have been determined. [3H]NMS bound with high affinity (Kd = 25 +/- 5.9 pM; n = 3) and to a high density Bmax = 11.8 +/- 0.025 nmol/g wet weight) of muscarinic receptors. [3H]OXO-M appeared to bind to two binding sites with differing affinities (Kd1 = 2.5 +/- 0.1 nM; Kd2 = 9.0 +/- 4.9 microM; n = 4) and to a different population of binding sites (Bmax1 = 5.0 +/- 0.26 nmol/g wet weight; Bmax2 = 130 +/- 60 nmol/g wet weight). Well known antagonists exhibited high affinity for [3H]NMS binding but a lower affinity for [3H]OXO-M binding. The opposite was true for acetylcholine and other known agonists. However, pilocarpine and McN-A-343 had similar affinities for sites labeled by both radioligands. Using the ratios of antagonist-to-agonist binding affinities, it was possible to group compounds into apparently distinct full agonist (ratios of 180-665; e.g. carbachol, muscarine, OXO-M, OXO-S and arecoline), partial agonist (ratios of 14-132; e.g. McN-A-343, pilocarpine, aceclidine, bethanechol, OXA-22 and acetylcholine) and antagonist (ratios of 0.22-1.9; e.g. atropine, NMS, pirenzepine, methoctramine, 4-DAMP and p-fluorohexahydrosialo-difenidol) classes. These data suggest that the NMS/OXO-M affinity ratios using a mixture of M1-M4 muscarinic receptors may be a useful way to screen and group a large number of compounds into apparent agonist, partial agonist, and antagonist classes of cholinergic agents.
MeSH term(s)	Animals ; Binding, Competitive ; Brain/metabolism ; Cholinergic Agents/classification ; Cholinergic Agents/metabolism ; Kinetics ; Lung/metabolism ; Muscarinic Agonists ; Muscarinic Antagonists ; Myocardium/metabolism ; N-Methylscopolamine ; Oxotremorine/metabolism ; Rabbits ; Radioligand Assay ; Rats ; Receptors, Muscarinic/metabolism ; Scopolamine Derivatives/metabolism ; Tritium
Chemical Substances	Cholinergic Agents ; Muscarinic Agonists ; Muscarinic Antagonists ; Receptors, Muscarinic ; Scopolamine Derivatives ; Tritium (10028-17-8) ; Oxotremorine (5RY0UWH1JL) ; N-Methylscopolamine (VDR09VTQ8U)
Language	English
Publishing date	1995-06
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	199335-5
ISSN	1573-6903 ; 0364-3190
ISSN (online)	1573-6903
ISSN	0364-3190
DOI	10.1007/bf01705534
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Article: Diabetes-Related Microvascular Complications in Primary Health Care Settings in the West Bank, Palestine.

Dweib, Mohammad / El Sharif, Nuha

Journal of clinical medicine

2023 Volume 12, Issue 21

Abstract: Background: Worldwide, retinopathy, nephropathy, and neuropathy are the major diabetes-related microvascular complications. In Palestine, a low-middle-income country, diabetes is the fourth reason for death. However, a few studies examined diabetes ... ...

Abstract	Background: Worldwide, retinopathy, nephropathy, and neuropathy are the major diabetes-related microvascular complications. In Palestine, a low-middle-income country, diabetes is the fourth reason for death. However, a few studies examined diabetes microvascular consequences and its management. Therefore, we carried out a national study that aims to investigate the factors associated with diabetes-related microvascular complications among individuals seeking care in primary healthcare settings of the West Bank of Palestine. Method: Using a cluster systematic sampling technique, 882 participants with diabetes patients were chosen for a cross-sectional study from primary healthcare facilities operated by the Ministry of Health (PMoH), the United Nations Relief and Works Agency (UNRWA), and the Palestinian Medical Relief Society (PMRS). Data about patients related to diabetes-related complications, medication use, and other diseases were extracted from patients' medical records. In addition, an interview face-to-face questionnaire was used to collect information about patients' sociodemographic variables, medical history, smoking habits, duration of the disease, presence of concurrent conditions previous referrals, and hospital admissions, as well as their level of knowledge regarding diabetes, complications, and treatments. Results: Approximately 34.4% of persons with diabetes patients in Palestine encounter at least one microvascular complication associated with diabetes. The most prevalent diabetes-related microvascular complication was retinopathy (17.3%), 23.4% of participants had more than one microvascular complication, and 29% of male patients had erectile dysfunction. A higher probability of having any microvascular complications was associated with older age (over 60 years). Participants with diabetes patients with fundoscopy or ophthalmology reports, according to diabetes follow-up guidelines, were less likely to develop retinopathy. Also, those who performed regular kidney function testing were less likely to have nephropathy, and those who performed a regular foot exam were less likely to develop diabetic foot. Conclusions: Diabetes-related microvascular complications were associated with patient age, low education level, residency location, and adherence to diabetes follow-up guidelines of diabetes management; i.e., having been tested for HbA1c, consulting with specialists, regular kidney function, and foot examination. These factors can be utilized in setting up proper management protocols to prevent or delay microvascular complications in many patients.
Language	English
Publishing date	2023-10-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12216719
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Article: Analyzing nonlinear oscillations with He's frequency-amplitude method and numerical comparison in jet engine vibration system.

Kawser, M Abul / Alim, Md Abdul / Sharif, Nazmul

Heliyon

2024 Volume 10, Issue 2, Page(s) e24261

Abstract: In this study, we describe and successfully solve a jet engine vibration equation using a straightforward tool known as the He's frequency-amplitude Method (HFAM). The jet engine vibration system demonstrates diverse applications across aerospace, power ... ...

Abstract	In this study, we describe and successfully solve a jet engine vibration equation using a straightforward tool known as the He's frequency-amplitude Method (HFAM). The jet engine vibration system demonstrates diverse applications across aerospace, power generation, industrial machinery, transportation, marine propulsion, energy optimization, defense, and aviation training. Utilizing HFAM, we derive periodic solutions in a general form for this system, considering various cases dependent on damping and driving forces. The obtained results highlight the effectiveness of HFAM as a distinct and straightforward technique for nonlinear equations. By comparing the solutions with numerical results obtained using the fourth-order Runge-Kutta method, we demonstrate the excellent accuracy of our solutions.
Language	English
Publishing date	2024-01-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e24261
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Article: Efficacy and Safety Outcomes of XEN Implantation and Gonioscopy-assisted Transluminal Trabeculotomy for the Management of Advanced Open-angle Glaucoma.

Ruparelia, Sunil / Sharif, Mohammed / Shoham-Hazon, Nir

Journal of current glaucoma practice

2023 Volume 17, Issue 2, Page(s) 63–67

Abstract: ... outcomes in patients with advanced OAG.: How to cite this article: Ruparelia S, Sharif M, Shoham-Hazon N ...

Abstract	Aim: Minimally invasive glaucoma surgery (MIGS) is not typically used in patients with advanced-stage glaucoma. This study describes the outcomes and complications of patients with advanced open-angle glaucoma (OAG) who underwent XEN implantation with cataract surgery or gonioscopy-assisted transluminal trabeculotomy (GATT) with cataract surgery. Methods: This retrospective study identified patients who had undergone XEN implantation or GATT for the management of advanced OAG. Outcomes included surgical success, intraocular pressure (IOP) reduction, number of topical IOP-lowering drops, visual field mean deviation (MD), best-corrected visual acuity (BCVA), and complications. Surgical success was defined as an IOP of <14 mm Hg and a 20% reduction at 12 months without topical IOP-lowering drops (complete success) or with topical IOP-lowering drops (qualified success). Results: Exactly 70 eyes were enrolled in this study, including 35 who had undergone XEN implantation and 35 who had undergone GATT. The overall surgical success rate was 74.3% (26 of 35) for eyes that underwent XEN implantation and 71.4% (25 of 35) for eyes that underwent GATT. Percent IOP reduction from baseline to 12 months postoperatively was 48% in the XEN cohort and 32% in the GATT cohort. Significant reduction in the use of topical IOP-lowering drops was demonstrated for both XEN (3.26 ± 1.15-1.23 ± 1.28) ( Conclusions: Both XEN implantation and GATT may be safe and effective management options when treating patients with advanced OAG. However, larger sample sizes are required to make direct statistical comparisons between these techniques. Clinical significance: In this study, XEN implantation and GATT combined with cataract surgery were each associated with favorable outcomes in patients with advanced OAG. How to cite this article: Ruparelia S, Sharif M, Shoham-Hazon N. Efficacy and Safety Outcomes of XEN Implantation and Gonioscopy-assisted Transluminal Trabeculotomy for the Management of Advanced Open-angle Glaucoma. J Curr Glaucoma Pract 2023;17(2):63-67.
Language	English
Publishing date	2023-06-16
Publishing country	India
Document type	Journal Article
ISSN	0974-0333
ISSN	0974-0333
DOI	10.5005/jp-journals-10078-1394
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Article ; Online: Exploring Potential Referral Pathways for Renal Artery Denervation and Developing a Centre of Excellence in Ireland.

Leahy, Niall / Wagener, Max / Shahzad, Shirjeel / Murphy, Darragh / McMorrow, Amy / Coen, Eileen / Sharif, Ruth / Sharif, Faisal

SN comprehensive clinical medicine

2024 Volume 6, Issue 1, Page(s) 32

Abstract: Arterial hypertension is one of the most significant and prevalent risk factors for cardiovascular disease. Despite widespread awareness of the condition, as well as a multitude of available antihypertensive drug classes, rates of uncontrolled ... ...

Abstract	Arterial hypertension is one of the most significant and prevalent risk factors for cardiovascular disease. Despite widespread awareness of the condition, as well as a multitude of available antihypertensive drug classes, rates of uncontrolled hypertension remain high on a global scale. Frequently, poor compliance with anti-hypertensive medication plays a big role in patients' inability to attain adequate blood pressure control. In individuals with resistant and/or uncontrolled hypertension, renal denervation is an emerging device-based therapy that has shown to be efficacious and safe in reducing blood pressure in several sham controlled trials. Additionally, it represents a treatment option for patients intolerant to oral pharmacotherapy. University Hospital Galway has been performing renal denervation procedures over the past number of years within multicentre, international sham-controlled trials and registries. Representing a novel and emerging antihypertensive treatment option, sources of referral for renal denervation are diverse and multiple; thus, there is an unmet need for standardised referral structures in Ireland. Herein, we review current and developing referral pathways for renal denervation at our institution, and discuss streamlined patient management and requirements to establish a centre of excellence.
Language	English
Publishing date	2024-03-16
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2947211-8
ISSN	2523-8973 ; 2523-8973
ISSN (online)	2523-8973
ISSN	2523-8973
DOI	10.1007/s42399-024-01647-9
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Article ; Online: Bilateral pneumothorax in a patient with angiosarcoma of the scalp: a case report.

Vaqar, Maham / Sharif, Ayesha / Iqbal, Nousheen / Irfan, Muhammad

Journal of medical case reports

2023 Volume 17, Issue 1, Page(s) 173

Abstract: Background: Angiosarcoma is a rare, vascular malignancy that arises from endothelial cells of blood vessels. This case report aims to create the awareness of its existence in the region and its mode of presentation.: Case presentation: A 63-year-old ... ...

Abstract	Background: Angiosarcoma is a rare, vascular malignancy that arises from endothelial cells of blood vessels. This case report aims to create the awareness of its existence in the region and its mode of presentation. Case presentation: A 63-year-old Pakistani man presented to the emergency department with sudden bilateral chest pain and shortness of breath for 2 days. On examination, a scalp lesion was seen which had been increasing in size over the last 6 weeks. The lesion was 8 × 10 cm in size with an irregular border, non-tender, violet and dome-shaped in elevation on the right occipito-parietal lobe of the skull. Chest computed tomography (CT) showed multiple cystic lesions on both lungs, patchy areas of ground-glass opacities, nodules of variable sizes and bilateral pneumothorax. Bilateral tube thoracostomy was performed which provided symptomatic relief for shortness of breath. His bronchoalveolar lavage (BAL) was negative for infection. He underwent biopsy of scalp lesion which was positive for aggressive angiosarcoma. Conclusion: Bilateral spontaneous pneumothorax can be the initial manifestation of aggressive cutaneous angiosarcoma and frequently leads to respiratory failure. Early recognition is essential to prevent delay in diagnosis and management.
MeSH term(s)	Male ; Humans ; Middle Aged ; Pneumothorax/diagnostic imaging ; Pneumothorax/etiology ; Pneumothorax/therapy ; Hemangiosarcoma/complications ; Hemangiosarcoma/diagnostic imaging ; Hemangiosarcoma/pathology ; Scalp/pathology ; Endothelial Cells/pathology ; Lung Neoplasms/pathology ; Dyspnea/etiology
Language	English
Publishing date	2023-05-01
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	2269805-X
ISSN	1752-1947 ; 1752-1947
ISSN (online)	1752-1947
ISSN	1752-1947
DOI	10.1186/s13256-023-03878-1
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Article: Tuberculosis detection from raw sputum samples using Au-electroplated screen-printed electrodes as E-DNA sensor.

Sharif, M N / Taufiq, S / Sohail, M / Abbas, S R

Frontiers in chemistry

2022 Volume 10, Page(s) 1046930

Abstract: Tuberculosis (TB) remains a leading cause of death globally, especially in underdeveloped nations. The main impediment to TB eradication is a lack of efficient diagnostic tools for disease diagnosis. In this work, label free and ultrasensitive ... ...

Abstract	Tuberculosis (TB) remains a leading cause of death globally, especially in underdeveloped nations. The main impediment to TB eradication is a lack of efficient diagnostic tools for disease diagnosis. In this work, label free and ultrasensitive electrochemical DNA biosensor for detecting
Language	English
Publishing date	2022-11-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711776-5
ISSN	2296-2646
ISSN	2296-2646
DOI	10.3389/fchem.2022.1046930
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Article ; Online: The Outcome of A Centralization Program in Biliary atresia: 20 years and beyond.

Davenport, Mark / Makin, Erica / Ong, Evelyn Gp / Sharif, Khalid / Dawrant, Michael / Alizai, Naved

Annals of surgery

2024

Abstract: ... born with BA and managed between January 1999 and December 2019. Death occurred without intervention (n ... 10, 1.1%) or were subject to primary transplant (n=26, 3.0%); leaving 831 (95.9%) infants ...

Abstract	Objective: Biliary atresia (BA) is a rare disease and reported outcomes of surgical management, typically a Kasai portoenterostomy (KPE), vary considerably across the world. Centralization has been proposed to improve this. Summary background data: A national centralization programme was started in Jan. 1999, involving 3 English units with co-located liver transplant facilities. As the program has now reached the 20-year point, the main aim was to update outcome statistics and identify trends. Methods: Prospective registry and database. The main measures of outcome were (i) time to KPE, (ii) Clearance of Jaundice (CoJ), defined as reaching a bilirubin value of <20µmol/L (≈1.5 mg/dL), and (iii) actuarial native liver survival (NLS) and overall survival (OS). Data are quoted as median (IQR) and non-parametric statistical comparison used with P<0.05 regarded as significant. Results: 867 infants were born with BA and managed between January 1999 and December 2019. Death occurred without intervention (n=10, 1.1%) or were subject to primary transplant (n=26, 3.0%); leaving 831 (95.9%) infants who underwent KPE at median age of 51 (IQR 39-64) days. Age at KPE reduced over the period (P=0.0001) becoming 48(35-57) days in the last 5-year era. CoJ was achieved in 505/831 (60.6%), also increasing over the period (P=0.002). 42 (5.0%) died post-KPE and 384 were transplanted, leaving 405 alive with their native livers at last follow-up. Of the 412 children transplanted, there were 23 (5.6%) deaths, leaving 387 alive. 5-year and 10-year native liver survival were 51.3% (95% CI 54.8-47.8) and 46.5% (95% CI 50.1 - 42.9) and overall survival were 91.5% (95% CI 93.2 - 89.4) and 90.5% (95% CI 92.3 - 88.2%) respectively. Conclusions: There have been continued improvements in efficiency over the period of centralization with a significant reduction in time to KPE and improved CoJ following KPE. Overall survival in this disease remains >90%.
Language	English
Publishing date	2024-03-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	340-2
ISSN	1528-1140 ; 0003-4932
ISSN (online)	1528-1140
ISSN	0003-4932
DOI	10.1097/SLA.0000000000006273
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