Article: Identification of T-cell-activating recombinant antigens shared among three candidate antileprosy vaccines, killed M. leprae, M. bovis BCG, and mycobacterium w.
1988 Volume 56, Issue 2, Page(s) 265–273
Abstract: ... BCG, and Mycobacterium w, was studied using T-cell lines and clones raised from BCG- and killed-M ... kDa M. leprae and M. tuberculosis antigens are present in M. leprae, BCG, and Mycobacterium w; 18-kDa ... M. leprae antigen is shared between M. leprae and Mycobacterium w, 13B3 M. leprae antigen is ...
Abstract | Antigenic crossreactivity among three candidate antileprosy vaccines, killed Mycobacterium leprae, BCG, and Mycobacterium w, was studied using T-cell lines and clones raised from BCG- and killed-M. leprae-vaccinated subjects. To identify the crossreactive antigens, the T-cell lines and clones were tested against Escherichia coli lysates containing 65-, 36-, 28-, 18-, and 14-kilodalton (kDa) and 13B3 M. leprae antigens and 65-, 19-, and 12-kDa M. tuberculosis antigens. The short-term T-cell lines, which compared to T-cell clones are easy to raise and maintain, were equally effective in identifying the T-cell-activating recombinant antigens. The reactivity pattern of the T-cell lines and the clones suggested that 65-kDa M. leprae and M. tuberculosis antigens are present in M. leprae, BCG, and Mycobacterium w; 18-kDa M. leprae antigen is shared between M. leprae and Mycobacterium w, 13B3 M. leprae antigen is possessed by M. leprae and BCG. These and other unidentified T-cell-activating antigens shared among candidate leprosy vaccines may be the basis for induction of in vivo sensitization to M. leprae antigens after vaccination with BCG or Mycobacterium w. |
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MeSH term(s) | Antigens, Bacterial/analysis ; Antigens, Bacterial/immunology ; Bacterial Vaccines/immunology ; Cell Line ; Clone Cells ; Cross Reactions ; Humans ; Lymphocyte Activation ; Mycobacterium/immunology ; Mycobacterium bovis/immunology ; Mycobacterium leprae/immunology ; Recombinant Proteins/analysis ; Recombinant Proteins/immunology ; T-Lymphocytes/immunology ; Vaccines, Synthetic/immunology |
Chemical Substances | Antigens, Bacterial ; Bacterial Vaccines ; Recombinant Proteins ; Vaccines, Synthetic |
Language | English |
Publishing date | 1988-06 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 982148-x |
ISSN | 1544-581X ; 0148-916X |
ISSN (online) | 1544-581X |
ISSN | 0148-916X |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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