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  1. Article ; Online: Autonomously Replicating RNAs of Bungowannah Pestivirus: E

    Dalmann, Anja / Reimann, Ilona / Wernike, Kerstin / Beer, Martin

    Journal of virology

    2020  Volume 94, Issue 14

    Abstract: Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, ... ...

    Abstract Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, E
    MeSH term(s) Gene Deletion ; Gene Expression ; Genes, Reporter ; Genetic Engineering ; Host-Pathogen Interactions ; Pestivirus/physiology ; Pestivirus Infections/immunology ; Pestivirus Infections/virology ; RNA, Viral ; Replicon ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/metabolism ; Virion ; Virus Assembly ; Virus Replication
    Chemical Substances RNA, Viral ; Viral Envelope Proteins
    Keywords covid19
    Language English
    Publishing date 2020-07-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.00436-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Thesis ; Online: Klonierung und Expression von Orthobunyavirus-Sequenzen zur Etablierung reverser genetischer Systeme und Analyse molekularer Virus-Wirt-Interaktionen

    Dalmann, Anja

    2015  

    Keywords ddc:570
    Language German
    Publishing country de
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Thesis ; Online: Klonierung und Expression von Orthobunyavirus-Sequenzen zur Etablierung reverser genetischer Systeme und Analyse molekularer Virus-Wirt-Interaktionen

    Dalmann, Anja

    2015  

    Keywords Text ; ddc:570
    Language German
    Publishing country de
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Seroprevalences of Newly Discovered Porcine Pestiviruses in German Pig Farms.

    Michelitsch, Anna / Dalmann, Anja / Wernike, Kerstin / Reimann, Ilona / Beer, Martin

    Veterinary sciences

    2019  Volume 6, Issue 4

    Abstract: Several novel porcine pestiviruses that are linked to disease outbreaks in commercial pig farms were discovered during recent years. Bungowannah pestivirus (BuPV; new ... ...

    Abstract Several novel porcine pestiviruses that are linked to disease outbreaks in commercial pig farms were discovered during recent years. Bungowannah pestivirus (BuPV; new species
    Language English
    Publishing date 2019-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci6040086
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Autonomously replicating RNAs of Bungowannah pestivirus

    Dalmann, Anja / Reimann, Ilona / Wernike, Kerstin / Beer, Martin

    ERNS is not essential for the generation of infectious particles

    2020  

    Abstract: Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, ERNS, E1 and E2 were constructed on the basis of an infectious cDNA clone of BuPV. Nanoluciferase ( ...

    Abstract Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, ERNS, E1 and E2 were constructed on the basis of an infectious cDNA clone of BuPV. Nanoluciferase (Nluc) insertion was used to compare the replication efficiency of all constructs after electroporation of in vitro-transcribed RNA from the different clones. Deletion of C, E1, E2 or the complete structural protein genome region (C-ERNS-E1-E2) prevented the production of infectious progeny virus, whereas deletion of ERNS still allowed the generation of infectious particles. However, those ΔERNS viral particles were defective in virus assembly and/or egress and could not be further propagated for more than three additional passages in porcine SK-6 cells. These “defective in third cycle” BuPV ΔERNS mutants were subsequently used to express the classical swine fever virus envelope protein E2, the N-terminal domain of the Schmallenberg virus Gc-protein and the receptor binding domain of the Middle-East Respiratory Syndrome Coronavirus spike protein. The constructs could be efficiently complemented and further passaged in SK-6 cells constitutively expressing the BuPV ERNS protein. Importantly, BuPV are able to infect a wide variety of target cell lines, allowing the expression in a very wide host spectrum. Therefore, we suggest that packaged BuPV ΔERNS replicon particles have the potential as broad spectrum viral vectors.
    Keywords Text ; ddc:570 ; Pestivirus ; Bungowannah virus ; ERNS53 ; replicon ; foreign gene expression ; 54 virus replicon particles ; viral vector
    Subject code 570
    Language English
    Publishing date 2020-05-13
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus

    Dalmann, Anja / Wernike, Kerstin / Snijder, Eric J / Oreshkova, Nadia / Reimann, Ilona / Beer, Martin

    Viruses. 2020 Aug. 04, v. 12, no. 8

    2020  

    Abstract: Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ... ...

    Abstract Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme sequence at the 5′ end and the hepatitis delta ribozyme at the 3′ end was placed under the control of the CMV RNA polymerase II promoter. Infectious recombinant BuPV could be rescued from pBuPV-DNA-transfected SK-6 cells and it had very similar growth characteristics to BuPV generated by conventional RNA-based reverse genetics and wild type BuPV. Subsequently, DNA-based EᴿᴺS deleted BuPV split genomes (pBuPV∆EᴿᴺS/EᴿᴺS)—co-expressing the EᴿᴺS protein from a separate synthetic CAG promoter—were constructed and characterized in vitro. Overall, DNA-launched BuPV genomes enable a rapid and cost-effective generation of recombinant BuPV and virus mutants, however, the protein expression efficiency of the DNA-launched systems after transfection is very low and needs further optimization in the future to allow the use e.g., as vaccine platform.
    Keywords DNA-directed RNA polymerase ; Pestivirus ; clones ; complementary DNA ; cost effectiveness ; genes ; hepatitis ; mutants ; protein synthesis ; reverse genetics ; ribozymes ; transfection ; vaccine development ; vaccines ; viruses
    Language English
    Dates of publication 2020-0804
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12080847
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Autonomously Replicating RNAs of Bungowannah Pestivirus: ERNS Is Not Essential for the Generation of Infectious Particles

    Dalmann, Anja / Reimann, Ilona / Wernike, Kerstin / Beer, Martin

    J. virol

    Abstract: Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, ERNS, E1, and E2 were constructed on the basis of an infectious cDNA clone of BuPV. Nanoluciferase ...

    Abstract Autonomously replicating subgenomic Bungowannah virus (BuPV) RNAs (BuPV replicons) with deletions of the genome regions encoding the structural proteins C, ERNS, E1, and E2 were constructed on the basis of an infectious cDNA clone of BuPV. Nanoluciferase (Nluc) insertion was used to compare the replication efficiencies of all constructs after electroporation of in vitro-transcribed RNA from the different clones. Deletion of C, E1, E2, or the complete structural protein genome region (C-ERNS-E1-E2) prevented the production of infectious progeny virus, whereas deletion of ERNS still allowed the generation of infectious particles. However, those ΔERNS viral particles were defective in virus assembly and/or egress and could not be further propagated for more than three additional passages in porcine SK-6 cells. These "defective-in-third-cycle" BuPV ΔERNS mutants were subsequently used to express the classical swine fever virus envelope protein E2, the N-terminal domain of the Schmallenberg virus Gc protein, and the receptor binding domain of the Middle East respiratory syndrome coronavirus spike protein. The constructs could be efficiently complemented and further passaged in SK-6 cells constitutively expressing the BuPV ERNS protein. Importantly, BuPVs are able to infect a wide variety of target cell lines, allowing expression in a very wide host spectrum. Therefore, we suggest that packaged BuPV ΔERNS replicon particles have potential as broad-spectrum viral vectors.IMPORTANCE The proteins NPRO and ERNS are unique for the genus Pestivirus, but only NPRO has been demonstrated to be nonessential for in vitro growth. While this was also speculated for ERNS, it has always been previously shown that pestivirus replicons with deletions of the structural proteins ERNS, E1, or E2 did not produce any infectious progeny virus in susceptible host cells. Here, we demonstrated for the first time that BuPV ERNS is dispensable for the generation of infectious virus particles but still important for efficient passaging. The ERNS-defective BuPV particles showed clearly limited growth in cell culture but were capable of several rounds of infection, expression of foreign genes, and highly efficient trans-complementation to rescue virus replicon particles (VRPs). The noncytopathic characteristics and the absence of preexisting immunity to BuPV in human populations and livestock also provide a significant benefit for a possible use, e.g., as a vector vaccine platform.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #260611
    Database COVID19

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  8. Article ; Online: Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus.

    Dalmann, Anja / Wernike, Kerstin / Snijder, Eric J / Oreshkova, Nadia / Reimann, Ilona / Beer, Martin

    Viruses

    2020  Volume 12, Issue 8

    Abstract: Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ... ...

    Abstract Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme sequence at the 5' end and the hepatitis delta ribozyme at the 3' end was placed under the control of the CMV RNA polymerase II promoter. Infectious recombinant BuPV could be rescued from pBuPV-DNA-transfected SK-6 cells and it had very similar growth characteristics to BuPV generated by conventional RNA-based reverse genetics and wild type BuPV. Subsequently, DNA-based E
    MeSH term(s) Animals ; Cell Line ; Cloning, Molecular ; Cytomegalovirus/genetics ; DNA, Complementary/genetics ; DNA-Directed DNA Polymerase/genetics ; Genome, Viral ; Pestivirus/genetics ; Pestivirus/pathogenicity ; Promoter Regions, Genetic ; RNA Polymerase II/genetics ; RNA, Catalytic/genetics ; Reverse Genetics/methods ; Swine
    Chemical Substances DNA, Complementary ; RNA, Catalytic ; RNA Polymerase II (EC 2.7.7.-) ; bacteriophage T7 induced DNA polymerase (EC 2.7.7.-) ; DNA-Directed DNA Polymerase (EC 2.7.7.7)
    Language English
    Publishing date 2020-08-04
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12080847
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book ; Audio / Video ; Online: Expression of viral immunogens by pestivirus replicon vectors

    Dalmann, Anja / Reimann, Ilona

    2016  

    Keywords ddc:570
    Language English
    Publishing date 2016-09-29
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  10. Book ; Audio / Video ; Online: Expression of viral immunogens by pestivirus replicon vectors

    Dalmann, Anja / Reimann, Ilona

    2016  

    Keywords Text ; ddc:570
    Language English
    Publishing date 2016-09-29
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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