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  1. Article ; Online: Methods to Determine the Role of Autophagy Proteins in C. elegans Aging.

    Henis-Korenblit, Sivan / Meléndez, Alicia

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1880, Page(s) 561–586

    Abstract: This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and ... ...

    Abstract This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and degradation by autophagy.
    MeSH term(s) Aging/physiology ; Animals ; Autophagy/physiology ; Autophagy-Related Proteins/analysis ; Autophagy-Related Proteins/genetics ; Autophagy-Related Proteins/metabolism ; Biological Assay/instrumentation ; Biological Assay/methods ; Caenorhabditis elegans/physiology ; Caenorhabditis elegans Proteins/analysis ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Gene Knockdown Techniques/instrumentation ; Gene Knockdown Techniques/methods ; Longevity/physiology ; Models, Animal ; RNA Interference
    Chemical Substances Autophagy-Related Proteins ; Caenorhabditis elegans Proteins
    Language English
    Publishing date 2018-10-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8873-0_37
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Non-autonomous autophagy in germline stem cell proliferation.

    Ames, Kristina / Meléndez, Alicia

    Cell cycle (Georgetown, Tex.)

    2017  Volume 16, Issue 16, Page(s) 1481–1482

    MeSH term(s) Autophagy ; Beclin-1 ; Cell Division ; Cell Proliferation ; Germ Cells
    Chemical Substances Beclin-1
    Language English
    Publishing date 2017-07-19
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2146183-1
    ISSN 1551-4005 ; 1538-4101 ; 1554-8627
    ISSN (online) 1551-4005
    ISSN 1538-4101 ; 1554-8627
    DOI 10.1080/15384101.2017.1345233
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5881: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Autophagy in C. elegans development.

    Palmisano, Nicholas J / Meléndez, Alicia

    Developmental biology

    2018  Volume 447, Issue 1, Page(s) 103–125

    Abstract: Autophagy involves the sequestration of cytoplasmic contents in a double-membrane structure referred to as the autophagosome and the degradation of its contents upon delivery to lysosomes. Autophagy activity has a role in multiple biological processes ... ...

    Abstract Autophagy involves the sequestration of cytoplasmic contents in a double-membrane structure referred to as the autophagosome and the degradation of its contents upon delivery to lysosomes. Autophagy activity has a role in multiple biological processes during the development of the nematode Caenorhabditis elegans. Basal levels of autophagy are required to remove aggregate prone proteins, paternal mitochondria, and spermatid-specific membranous organelles. During larval development, autophagy is required for the remodeling that occurs during dauer development, and autophagy can selectively degrade components of the miRNA-induced silencing complex, and modulate miRNA-mediated silencing. Basal levels of autophagy are important in synapse formation and in the germ line, to promote the proliferation of proliferating stem cells. Autophagy activity is also required for the efficient removal of apoptotic cell corpses by promoting phagosome maturation. Finally, autophagy is also involved in lipid homeostasis and in the aging process. In this review, we first describe the molecular complexes involved in the process of autophagy, its regulation, and mechanisms for cargo recognition. In the second section, we discuss the developmental contexts where autophagy has been shown to be important. Studies in C. elegans provide valuable insights into the physiological relevance of this process during metazoan development.
    MeSH term(s) Animals ; Autophagy/physiology ; Caenorhabditis elegans/embryology ; Caenorhabditis elegans Proteins/metabolism ; MicroRNAs/metabolism ; RNA, Helminth/metabolism
    Chemical Substances Caenorhabditis elegans Proteins ; MicroRNAs ; RNA, Helminth
    Language English
    Publishing date 2018-04-27
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1114-9
    ISSN 1095-564X ; 0012-1606
    ISSN (online) 1095-564X
    ISSN 0012-1606
    DOI 10.1016/j.ydbio.2018.04.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 508: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Breast-Conserving Surgery Guided with Magnetic Seeds vs. Wires: A Single-Institution Experience.

    Moreno-Palacios, Elisa / Martí, Covadonga / Frías, Laura / Meléndez, Marcos / Loayza, Adolfo / Roca, María José / Córdoba, Vicenta / Oliver, José María / Hernández, Alicia / Sánchez-Méndez, José Ignacio

    Cancers

    2024  Volume 16, Issue 3

    Abstract: Purpose: The aim of this study is to describe our initial experience using magnetic seeds (Magseed: Methods: We performed a retrospective study including all breast-conserving surgeries for non-palpable breast lesions under 16 mm from June 2018 to ... ...

    Abstract Purpose: The aim of this study is to describe our initial experience using magnetic seeds (Magseed
    Methods: We performed a retrospective study including all breast-conserving surgeries for non-palpable breast lesions under 16 mm from June 2018 to May 2021. We compared breast-conserving surgeries guided with magnetic seeds (Magseed
    Results: Data from 225 cases were collected, including 149 cases guided by magnetic seeds and 76 cases guided by wires. The breast lesion was localized in every case. Both cohorts were similar regarding clinical and pathological characteristics. We found significant statistical differences (
    Conclusion: In our experience, the use of magnetic seed (Magseed
    Language English
    Publishing date 2024-01-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16030566
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Synthesis and Characterization of a pH- and Temperature-Sensitive Fe

    Sánchez-Orozco, Jorge Luis / Meléndez-Ortiz, Héctor Iván / Puente-Urbina, Bertha Alicia / Rodríguez-Fernández, Oliverio Santiago / Martínez-Luévanos, Antonia / García-Cerda, Luis Alfonso

    Polymers

    2023  Volume 15, Issue 4

    Abstract: This work reports the synthesis, characterization, and in vitro release studies of pH- and temperature-sensitive ... ...

    Abstract This work reports the synthesis, characterization, and in vitro release studies of pH- and temperature-sensitive Fe
    Language English
    Publishing date 2023-02-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym15040968
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: RNAi-Mediated Inactivation of Autophagy Genes in Caenorhabditis elegans.

    Palmisano, Nicholas J / Meléndez, Alicia

    Cold Spring Harbor protocols

    2016  Volume 2016, Issue 2, Page(s) pdb.prot086520

    Abstract: RNA interference (RNAi) is a process that results in the sequence-specific silencing of endogenous mRNA through the introduction of double-stranded RNA (dsRNA). In the nematode Caenorhabditis elegans, RNA inactivation can be used at any specific ... ...

    Abstract RNA interference (RNAi) is a process that results in the sequence-specific silencing of endogenous mRNA through the introduction of double-stranded RNA (dsRNA). In the nematode Caenorhabditis elegans, RNA inactivation can be used at any specific developmental stage or during adulthood to inhibit a given target gene. Investigators can take advantage of the fact that, in C. elegans, RNAi is unusual in that it is systemic, meaning that dsRNA can spread throughout the animal and can affect virtually all tissues except neurons. Here, we describe a protocol for the most common method to achieve RNAi in C. elegans, which is to feed them bacteria that express dsRNA complementary to a specific target gene. This method has various advantages, including the availability of libraries that essentially cover the whole genome, the ability to treat animals at any developmental stage, and that it is relatively cost effective. We also discuss how RNAi specific to autophagy genes has proven to be an excellent method to study the role of these genes in autophagy, as well as other cellular and developmental processes, while also highlighting the caveats that must be applied.
    MeSH term(s) Animals ; Autophagy ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/physiology ; Gene Knockdown Techniques/methods ; Genes, Helminth ; RNA Interference
    Language English
    Publishing date 2016-02-01
    Publishing country United States
    Document type Journal Article
    ISSN 1559-6095
    ISSN (online) 1559-6095
    DOI 10.1101/pdb.prot086520
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Detection of Autophagy in Caenorhabditis elegans.

    Palmisano, Nicholas J / Meléndez, Alicia

    Cold Spring Harbor protocols

    2016  Volume 2016, Issue 2, Page(s) pdb.top070466

    Abstract: Autophagy is a dynamic and catabolic process that results in the breakdown and recycling of cellular components through the autophagosomal-lysosomal pathway. Many autophagy genes identified in yeasts and mammals have orthologs in the nematode ... ...

    Abstract Autophagy is a dynamic and catabolic process that results in the breakdown and recycling of cellular components through the autophagosomal-lysosomal pathway. Many autophagy genes identified in yeasts and mammals have orthologs in the nematode Caenorhabditis elegans. In recent years, gene inactivation by RNA interference (RNAi) and chromosomal mutations has been useful to probe the functions of autophagy in C. elegans, and a role for autophagy has been shown to contribute to multiple processes, such as the adaptation to stress, longevity, cell death, cell growth control, clearance of aggregation-prone proteins, degradation of P granules during embryogenesis, and apoptotic cell clearance. Here, we discuss some of these roles and describe methods that can be used to study autophagy in C. elegans. Specifically, we summarize how to visualize autophagy in embryos, larva, or adults, how to detect the lipidation of the ubiquitin-like modifier LGG-1 by western blot, and how to inactivate autophagy genes by RNAi.
    MeSH term(s) Animals ; Autophagy ; Blotting, Western/methods ; Caenorhabditis elegans/physiology ; Gene Knockdown Techniques ; Molecular Biology/methods ; Optical Imaging/methods ; Parasitology/methods ; RNA Interference
    Language English
    Publishing date 2016-02-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1559-6095
    ISSN (online) 1559-6095
    DOI 10.1101/pdb.top070466
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Detecting Autophagy in Caenorhabditis elegans Embryos Using Markers of P Granule Degradation.

    Palmisano, Nicholas J / Meléndez, Alicia

    Cold Spring Harbor protocols

    2016  Volume 2016, Issue 1, Page(s) pdb.prot086504

    Abstract: Autophagy plays an active role during the early stages of embryogenesis in the nematode Caenorhabditis elegans. Although their exact function is unknown, P granules are ribonucleoprotein particles that play a role in germ cell specification. The ... ...

    Abstract Autophagy plays an active role during the early stages of embryogenesis in the nematode Caenorhabditis elegans. Although their exact function is unknown, P granules are ribonucleoprotein particles that play a role in germ cell specification. The localization of P granules is restricted to the germline precursor cells in wild-type embryos, as a result of their degradation in the somatic cell lineage. Autophagy is known to be required for the degradation of P granules, as mutations in various autophagy genes, including those encoding the adaptor SEPA-1 and the p62-like adaptor SQST-1, result in the accumulation of the P granule components PGL-1 and PGL-3 (termed PGL granules) in the somatic cells of C. elegans embryos. In this protocol, we present a methodology for using fusion reporters of SEPA-1, SQST-1, and PGL-1 that have aided in the identification of new genes for normal autophagy activity by screening for mutant animals that lack the degradation of these autophagy substrates.
    MeSH term(s) Animals ; Autophagy/physiology ; Caenorhabditis elegans/cytology ; Caenorhabditis elegans/embryology ; Embryonic Development/genetics ; Embryonic Development/physiology ; Germ Cells/pathology ; Proteolysis ; RNA-Binding Proteins/metabolism
    Chemical Substances RNA-Binding Proteins
    Language English
    Publishing date 2016-01-04
    Publishing country United States
    Document type Journal Article
    ISSN 1559-6095
    ISSN (online) 1559-6095
    DOI 10.1101/pdb.prot086504
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Autophagy in C. elegans development

    Palmisano, Nicholas J / Meléndez, Alicia

    Developmental biology. 2019 Mar. 01, v. 447, no. 1

    2019  

    Abstract: Autophagy involves the sequestration of cytoplasmic contents in a double-membrane structure referred to as the autophagosome and the degradation of its contents upon delivery to lysosomes. Autophagy activity has a role in multiple biological processes ... ...

    Abstract Autophagy involves the sequestration of cytoplasmic contents in a double-membrane structure referred to as the autophagosome and the degradation of its contents upon delivery to lysosomes. Autophagy activity has a role in multiple biological processes during the development of the nematode Caenorhabditis elegans. Basal levels of autophagy are required to remove aggregate prone proteins, paternal mitochondria, and spermatid-specific membranous organelles. During larval development, autophagy is required for the remodeling that occurs during dauer development, and autophagy can selectively degrade components of the miRNA-induced silencing complex, and modulate miRNA-mediated silencing. Basal levels of autophagy are important in synapse formation and in the germ line, to promote the proliferation of proliferating stem cells. Autophagy activity is also required for the efficient removal of apoptotic cell corpses by promoting phagosome maturation. Finally, autophagy is also involved in lipid homeostasis and in the aging process. In this review, we first describe the molecular complexes involved in the process of autophagy, its regulation, and mechanisms for cargo recognition. In the second section, we discuss the developmental contexts where autophagy has been shown to be important. Studies in C. elegans provide valuable insights into the physiological relevance of this process during metazoan development.
    Keywords Caenorhabditis elegans ; apoptosis ; autophagy ; germ cells ; homeostasis ; larval development ; lipids ; lysosomes ; mitochondria ; phagosomes ; proteins ; stem cells ; synapse
    Language English
    Dates of publication 2019-0301
    Size p. 103-125.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1114-9
    ISSN 1095-564X ; 0012-1606
    ISSN (online) 1095-564X
    ISSN 0012-1606
    DOI 10.1016/j.ydbio.2018.04.009
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 76/715: Show issues

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  10. Article ; Online: Detection of Autophagy in Caenorhabditis elegans by Western Blotting Analysis of LGG-1.

    Palmisano, Nicholas J / Meléndez, Alicia

    Cold Spring Harbor protocols

    2016  Volume 2016, Issue 2, Page(s) pdb.prot086512

    Abstract: A common way to measure the induction of autophagy in yeast and mammalian cells is to compare the amount of Atg8/LC3-I with that of Atg8-PE/LC3-II by using western blot analysis. This is because changes in the amount of LC3-II correlate closely with ... ...

    Abstract A common way to measure the induction of autophagy in yeast and mammalian cells is to compare the amount of Atg8/LC3-I with that of Atg8-PE/LC3-II by using western blot analysis. This is because changes in the amount of LC3-II correlate closely with changes in the number of autophagosomes present in cells. Atg8/LC3 is initially synthesized as an unprocessed form, which is proteolytically processed to form Atg8/LC3-I, and then this is modified into the phosphatidylethanolamine (PE)-conjugated Atg8-PE/LC3-II form. Atg8/LC3-II is membrane bound, whereas Atg8-PE/LC3-I is cytosolic. By associating with both the inner and outer membranes of the autophagosome, Atg8-PE/LC3-II is the only autophagy reporter that is reliably associated with completed autophagosomes. In the nematode Caenorhabditis elegans, the ortholog of Atg8/LC3 is LGG-1. Here, we discuss how changes in the levels of LGG-1-II (and the paralog LGG-2) protein can, with appropriate controls, be used to monitor autophagy activity in nematodes and present a protocol for monitoring changes in the protein levels of different forms of LGG-1 by western blotting.
    MeSH term(s) Animals ; Autophagy ; Blotting, Western/methods ; Caenorhabditis elegans/cytology ; Caenorhabditis elegans/physiology ; Gene Silencing ; Molecular Biology/methods ; Optical Imaging/methods ; Parasitology/methods
    Language English
    Publishing date 2016-02-01
    Publishing country United States
    Document type Journal Article
    ISSN 1559-6095
    ISSN (online) 1559-6095
    DOI 10.1101/pdb.prot086512
    Database MEDical Literature Analysis and Retrieval System OnLINE

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