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Article ; Online: Construction of a tubular scaffold that mimics J-shaped stress/strain mechanics using an innovative electrospinning technique.

Rapoport, Harry Scott / Fish, Jeff / Basu, Joydeep / Campbell, Jonathon / Genheimer, Christopher / Payne, Richard / Jain, Deepak

Tissue engineering. Part C, Methods

2012 Volume 18, Issue 8, Page(s) 567–574

Abstract: ... characterized by a "J"-shaped curve on a stress-strain diagram with a low-stiffness highly elastic zone giving ... formation in tissues whose primary loading is pressure-based. "J"-shaped behavior arises ... of the corrugated laminae seen in blood vessels. In doing so, tubular scaffolds have been fabricated with complex "J ...

Abstract	Soft tissues such as blood vessel, lung, ureter, skin, etc., possess mechanical behavior characterized by a "J"-shaped curve on a stress-strain diagram with a low-stiffness highly elastic zone giving rise to a high-stiffness zone. This mechanical behavior may be adaptive and protective against aneurysm formation in tissues whose primary loading is pressure-based. "J"-shaped behavior arises from the synergistic interplay of two main structural proteins: collagen and elastin. An innovative electrospinning technique has been utilized to form tubular scaffold composites with structural features reminiscent of the corrugated laminae seen in blood vessels. In doing so, tubular scaffolds have been fabricated with complex "J"-shaped behavior through the use of elastic polyurethane and reinforcing poly-glycolic acid (PGA) woven mesh. In these studies, corrugated laminae were formed on the 175 μm and 1.5 mm scale. Initial moduli were 0.5±0.17 MPa (mean±standard deviation) giving rise to stiffer moduli of 36.09±6.72 MPa at a strain of 1.31±0.15. Burst pressures were physiologically relevant at 3095±1016 mmHg. The toughness of these prototypes was 6.3±1.9 MJ/m(3). The ability to employ different materials and different formation parameters utilizing this technique promises the ability to match complex stress-strain behaviors in soft tissues with a high degree of fidelity.
MeSH term(s)	Animals ; Elasticity ; Electrochemistry/methods ; Equipment Design ; Female ; Humans ; Male ; Materials Testing ; Polyglycolic Acid/chemistry ; Polyurethanes/chemistry ; Pressure ; Stress, Mechanical ; Tensile Strength ; Tissue Engineering/methods ; Tissue Scaffolds
Chemical Substances	Polyurethanes ; Polyglycolic Acid (26009-03-0)
Language	English
Publishing date	2012-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	2420585-0
ISSN	1937-3392 ; 1937-3384
ISSN (online)	1937-3392
ISSN	1937-3384
DOI	10.1089/ten.TEC.2011.0286
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Zs.A 5500/C: Show issues

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Book ; Online: Fast Facts : Comprehensive Genomic Profiling

Rapoport, Bernardo L. / Troncone, Giancarlo / Schmitt, Fernando / Nayler, Simon J.

Making precision medicine possible

2020

Abstract: Cancer is a multifaceted disease in which genetic changes induce uncontrolled tumor growth. Genomic characterization of cancer is now leading to better diagnostic, prognostic and predictive biomarkers, and effective individualized management. 'Fast Facts: ...

Title variant	Comprehensive Genomic Profiling
Author's details	Bernardo L. Rapoport, Giancarlo Troncone, Fernando Schmitt, Simon Nayler
Abstract	Cancer is a multifaceted disease in which genetic changes induce uncontrolled tumor growth. Genomic characterization of cancer is now leading to better diagnostic, prognostic and predictive biomarkers, and effective individualized management. 'Fast Facts: Comprehensive Genomic Profiling' provides a crash course in the science, methods and application of genomic profiling. Assuming only the most basic knowledge – or memory – of cell biology, the authors provide an overview of DNA and RNA biology and next-generation sequencing. This sets in context the descriptions of prognostic and predictive biomarkers for different cancer types and genomic-based treatments. Finally, but importantly, some of the practicalities of gaining and interpreting genomic information are described. Whether you need a primer or a refresher, this short colorful book demystifies this complex subject. Contents: • Genetic mutations and biomarkers • Understanding next-generation sequencing • Elements of comprehensive genomic profiles • Role in precision oncology • Predictive and prognostic biomarkers • Overcoming barriers to genotype-directed therapy
MeSH term(s)	Neoplasms/genetics. ; DNA Fingerprinting/methods.
Keywords	Oncology ; Biochemical markers
Language	English
Size	1 online resource (108 pages) :, 17 figures, 17 in color, 13 tables
Publisher	S. Karger
Publishing place	Basel
Document type	Book ; Online
ISBN	3-318-06819-5 ; 3-318-06818-7 ; 978-3-318-06819-1 ; 978-3-318-06818-4
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article: Interaction of BiP with the J-domain of the Sec63p component of the endoplasmic reticulum protein translocation complex.

Misselwitz, B / Staeck, O / Matlack, K E / Rapoport, T A

The Journal of biological chemistry

1999 Volume 274, Issue 29, Page(s) 20110–20115

Abstract: Proteins of the Hsp70 family of ATPases interact with a conserved domain of their J-protein ... partners, the J-domain, to function in numerous cellular processes. We have studied the interaction of BiP ... an Hsp70 family member in the lumen of the endoplasmic reticulum, with the J-domain of Sec63p, a component ...

Abstract	Proteins of the Hsp70 family of ATPases interact with a conserved domain of their J-protein partners, the J-domain, to function in numerous cellular processes. We have studied the interaction of BiP, an Hsp70 family member in the lumen of the endoplasmic reticulum, with the J-domain of Sec63p, a component of the Sec complex involved in post-translational protein translocation across the endoplasmic reticulum membrane. In a real-time solid phase binding assay, BiP binds to the immobilized Sec complex or to a fusion protein of the J-domain and glutathione S-transferase in a reaction that requires ATP hydrolysis. In the final complex, BiP is bound in the ADP form with its peptide binding pocket occupied. An intact peptide binding pocket is required for this interaction. Our experiments suggest that the activation of BiP by the J-domain involves a transient contact between these components, and that in the absence of physiological substrates, J-activated BiP binds even to the J-proteins themselves.
MeSH term(s)	Adenosine Triphosphatases/metabolism ; Adenosine Triphosphate/metabolism ; Amino Acid Sequence ; Base Sequence ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; DNA Primers ; Endoplasmic Reticulum/metabolism ; Enzyme Activation ; Fungal Proteins/chemistry ; Fungal Proteins/metabolism ; Glutathione Transferase/metabolism ; Heat-Shock Proteins ; Hydrolysis ; Membrane Proteins/chemistry ; Membrane Proteins/metabolism ; Membrane Transport Proteins ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Mutagenesis ; Peptides/metabolism ; Protein Binding ; Saccharomyces cerevisiae Proteins
Chemical Substances	Carrier Proteins ; DNA Primers ; Fungal Proteins ; Heat-Shock Proteins ; Membrane Proteins ; Membrane Transport Proteins ; Molecular Chaperones ; Peptides ; SEC63 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; Adenosine Triphosphate (8L70Q75FXE) ; Glutathione Transferase (EC 2.5.1.18) ; Adenosine Triphosphatases (EC 3.6.1.-) ; molecular chaperone GRP78 (YCYIS6GADR)
Language	English
Publishing date	1999-07-16
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.274.29.20110
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Article: J proteins catalytically activate Hsp70 molecules to trap a wide range of peptide sequences.

Misselwitz, B / Staeck, O / Rapoport, T A

Molecular cell

1998 Volume 2, Issue 5, Page(s) 593–603

Abstract: Proteins of the Hsp70 family of ATPases, such as BiP, function together with J proteins to bind ... segment of the J proteins, the J domain, catalytically activates BiP molecules to bind peptides ... in its immediate vicinity. The J domain interacts with the ATP form of BiP and stimulates hydrolysis resulting ...

Abstract	Proteins of the Hsp70 family of ATPases, such as BiP, function together with J proteins to bind polypeptides in numerous cellular processes. Using a solid phase binding assay, we demonstrate that a conserved segment of the J proteins, the J domain, catalytically activates BiP molecules to bind peptides in its immediate vicinity. The J domain interacts with the ATP form of BiP and stimulates hydrolysis resulting in the rapid trapping of peptides, which are then only slowly released upon nucleotide exchange. Activation by the J domain allows BiP to trap peptides or proteins that it would not bind on its own. These results explain why BiP and probably all other Hsp70s can interact with a wide range of substrates and suggest that the J partner primarily determines the substrate specificity of Hsp70s.
MeSH term(s)	Adenosine Diphosphate/metabolism ; Adenosine Triphosphatases/metabolism ; Adenosine Triphosphate/metabolism ; Binding Sites ; Chromatography, High Pressure Liquid ; Enzyme Activation ; Fungal Proteins/chemistry ; Fungal Proteins/isolation & purification ; Fungal Proteins/metabolism ; HSP70 Heat-Shock Proteins/isolation & purification ; HSP70 Heat-Shock Proteins/metabolism ; Heat-Shock Proteins ; Kinetics ; Membrane Proteins/chemistry ; Membrane Proteins/metabolism ; Membrane Transport Proteins ; Models, Biological ; Nucleotides/metabolism ; Peptides/chemical synthesis ; Peptides/metabolism ; Protein Conformation ; Saccharomyces cerevisiae Proteins ; Substrate Specificity ; Surface Plasmon Resonance
Chemical Substances	Fungal Proteins ; HSP70 Heat-Shock Proteins ; Heat-Shock Proteins ; KAR2 protein, yeast ; Membrane Proteins ; Membrane Transport Proteins ; Nucleotides ; Peptides ; SEC63 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; Adenosine Diphosphate (61D2G4IYVH) ; Adenosine Triphosphate (8L70Q75FXE) ; Adenosine Triphosphatases (EC 3.6.1.-)
Language	English
Publishing date	1998-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1415236-8
ISSN	1097-4164 ; 1097-2765
ISSN (online)	1097-4164
ISSN	1097-2765
DOI	10.1016/s1097-2765(00)80158-6
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Zs.A 5055: Show issues

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Article: A Fatal Case of Ruxolitinib Discontinuation Syndrome Preceded by Hypercalcemia.

Perl, Kobi / Epshtein, Ksenya / Rapoport, Micha J

The Israel Medical Association journal : IMAJ

2023 Volume 25, Issue 5, Page(s) 377–378

MeSH term(s)	Humans ; Hypercalcemia/chemically induced ; Nitriles ; Pyrazoles/adverse effects ; Pyrimidines
Chemical Substances	Nitriles ; Pyrazoles ; Pyrimidines ; ruxolitinib (82S8X8XX8H)
Language	English
Publishing date	2023-05-27
Publishing country	Israel
Document type	Case Reports ; Journal Article
ZDB-ID	2008291-5
ISSN	1565-1088 ; 0021-2180
ISSN	1565-1088 ; 0021-2180
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Zs.A 5470: Show issues

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Article ; Online: Harnessing the Power of Low-tech Collaborative Learning.

Sheth, Monica / Samreen, Naziya / Rapoport, Irina / Slanetz, Priscilla J / Fornari, Alice / Lewis, Petra

Journal of breast imaging

2024 Volume 2, Issue 6, Page(s) 609–614

Abstract: Medical education in the United States has undergone a paradigm shift from passive learning to more interactive student-centered teaching methods. Several digital tools and platforms have been developed to assist educators in creating a high-tech, ... ...

Abstract	Medical education in the United States has undergone a paradigm shift from passive learning to more interactive student-centered teaching methods. Several digital tools and platforms have been developed to assist educators in creating a high-tech, interactive classroom. However, there are many low-tech ways to engage learners and improve retention by combining collaborative learning techniques and summary exercises. Collaborative learning is an educational approach that involves the coordinated engagement of two or more learners for the purpose of completing a task or solving a problem. Such methods use a student-centered active learning model to promote higher-order cognitive tasks through active engagement in course content. Additionally, summary exercises at the end of a learning activity promote reflection and retention of learned concepts while clarifying content that may have been confusing for the learner. The purpose of this article is to describe the methodology and tips for the implementation of low-tech collaborative learning methods and summary activities into trainee educational activities to create an engaging student-centered learning environment.
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ISSN	2631-6129
ISSN (online)	2631-6129
DOI	10.1093/jbi/wbaa054
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Article ; Online: Airway protection and outcomes after staged versus concurrent bilateral neck dissections with transoral base of tongue cancer resection.

Lee, Jake J / Rapoport, Nicholas A / Pipkorn, Patrik / Puram, Sidharth V / Jackson, Ryan S

Head & neck

2024 Volume 46, Issue 5, Page(s) 1020–1027

Abstract: Objectives: To assess airway, safety, and resource utilization outcomes between transoral base of tongue (BOT) surgery with staged versus concurrent bilateral neck dissections (BND).: Methods: A retrospective cohort study of patients with human ... ...

Abstract	Objectives: To assess airway, safety, and resource utilization outcomes between transoral base of tongue (BOT) surgery with staged versus concurrent bilateral neck dissections (BND). Methods: A retrospective cohort study of patients with human papilloma virus (HPV)-related BOT cancer who underwent transoral surgery and BND from January 2015 through June 2022 was conducted. Free flap patients were excluded. Results: Of 126 patients (46 [37%] staged and 80 [63%] concurrent BND), there were no significant differences in rates of postoperative intubation, tracheostomy, intensive care admission, operative takebacks, gastrostomy, and 30-day readmission. Total operative time (median difference 1.4 [95% CI 0.9-1.8] hours), length of stay (1.0 [1.0-1.0] day), and time between primary surgery and adjuvant therapy initiation (4.0 [0.0-8.0] days) were lower in the concurrent BND cohort. Conclusion: Concurrent BND alongside transoral BOT resection is safe with similar airway outcomes and lower total operative time, length of stay, and time to adjuvant therapy initiation compared to staged BND.
MeSH term(s)	Humans ; Tongue Neoplasms/surgery ; Retrospective Studies ; Neck Dissection ; Carcinoma, Squamous Cell/surgery ; Oropharyngeal Neoplasms ; Robotic Surgical Procedures
Language	English
Publishing date	2024-02-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	645165-2
ISSN	1097-0347 ; 0148-6403 ; 1043-3074
ISSN (online)	1097-0347
ISSN	0148-6403 ; 1043-3074
DOI	10.1002/hed.27696
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Zs.A 1493: Show issues

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Article ; Online: Pro-Tumorigenic and Thrombotic Activities of Platelets in Lung Cancer.

Anderson, Ronald / Rapoport, Bernardo L / Steel, Helen C / Theron, Annette J

International journal of molecular sciences

2023 Volume 24, Issue 15

Abstract: Aside from their key protective roles in hemostasis and innate immunity, platelets are now recognized as having multifaceted, adverse roles in the pathogenesis, progression and outcome of many types of human malignancy. The most consistent and compelling ...

Abstract	Aside from their key protective roles in hemostasis and innate immunity, platelets are now recognized as having multifaceted, adverse roles in the pathogenesis, progression and outcome of many types of human malignancy. The most consistent and compelling evidence in this context has been derived from the notable association of elevated circulating platelet counts with the onset and prognosis of various human malignancies, particularly lung cancer, which represents the primary focus of the current review. Key topics include an overview of the association of lung cancer with the circulating platelet count, as well as the mechanisms of platelet-mediated, pro-tumorigenic immunosuppression, particularly the role of transforming growth factor beta 1. These issues are followed by a discussion regarding the pro-tumorigenic role of platelet-derived microparticles (PMPs), the most abundant type of microparticles (MPs) in human blood. In this context, the presence of increased levels of PMPs in the blood of lung cancer patients has been associated with tumor growth, invasion, angiogenesis and metastasis, which correlate with disease progression and decreased survival times. The final section of the review addresses, firstly, the role of cancer-related platelet activation and thrombosis in the pathogenesis of secondary cardiovascular disorders and the associated mortality, particularly in lung cancer, which is second only to disease progression; secondly, the review addresses the potential role of antiplatelet agents in the adjunctive therapy of cancer.
MeSH term(s)	Humans ; Blood Platelets/metabolism ; Cell-Derived Microparticles/metabolism ; Thrombosis/metabolism ; Lung Neoplasms/metabolism ; Carcinogenesis/metabolism ; Disease Progression
Language	English
Publishing date	2023-07-25
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241511927
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Article ; Online: Introducing surgical intelligence in gynecology: Automated identification of key steps in hysterectomy.

Levin, Ishai / Rapoport Ferman, Judith / Bar, Omri / Ben Ayoun, Danielle / Cohen, Aviad / Wolf, Tamir

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics

2024

Abstract: Objective: The analysis of surgical videos using artificial intelligence holds great promise for the future of surgery by facilitating the development of surgical best practices, identifying key pitfalls, enhancing situational awareness, and ... ...

Abstract	Objective: The analysis of surgical videos using artificial intelligence holds great promise for the future of surgery by facilitating the development of surgical best practices, identifying key pitfalls, enhancing situational awareness, and disseminating that information via real-time, intraoperative decision-making. The objective of the present study was to examine the feasibility and accuracy of a novel computer vision algorithm for hysterectomy surgical step identification. Methods: This was a retrospective study conducted on surgical videos of laparoscopic hysterectomies performed in 277 patients in five medical centers. We used a surgical intelligence platform (Theator Inc.) that employs advanced computer vision and AI technology to automatically capture video data during surgery, deidentify, and upload procedures to a secure cloud infrastructure. Videos were manually annotated with sequential steps of surgery by a team of annotation specialists. Subsequently, a computer vision system was trained to perform automated step detection in hysterectomy. Analyzing automated video annotations in comparison to manual human annotations was used to determine accuracy. Results: The mean duration of the videos was 103 ± 43 min. Accuracy between AI-based predictions and manual human annotations was 93.1% on average. Accuracy was highest for the dissection and mobilization step (96.9%) and lowest for the adhesiolysis step (70.3%). Conclusion: The results of the present study demonstrate that a novel AI-based model achieves high accuracy for automated steps identification in hysterectomy. This lays the foundations for the next phase of AI, focused on real-time clinical decision support and prediction of outcome measures, to optimize surgeon workflow and elevate patient care.
Language	English
Publishing date	2024-03-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	80149-5
ISSN	1879-3479 ; 0020-7292
ISSN (online)	1879-3479
ISSN	0020-7292
DOI	10.1002/ijgo.15490
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Zs.A 355: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 183: Show issues

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Article ; Online: An Echocardiographic Approach for the Management of Shock: The Subcostal to Apical, Respiratory to Parasternal-Cardiac to Respiratory, Aortic to Stomach Protocol.

Nikravan, Sara / Bughrara, Nibras / Klick, John / Lanspa, Michael J / Rapoport, Lisa / Díaz-Gomez, José

Seminars in ultrasound, CT, and MR

2023 Volume 45, Issue 1, Page(s) 74–83

Abstract: Point of care ultrasound has become an integral part of critical care medicine, particularly for recognizing shock etiologies and guiding management. Most of the current ultrasonography guided shock protocols have been tailored towards a qualitative ... ...

Abstract	Point of care ultrasound has become an integral part of critical care medicine, particularly for recognizing shock etiologies and guiding management. Most of the current ultrasonography guided shock protocols have been tailored towards a qualitative assessment of patients on presentation with shock. Unfortunately, the evolving nature of shock, particularly in the face of resuscitation and physiologic changes, demands a more sophisticated approach. This manuscript serves to present a comprehensive algorithm called the transthoracic Subcostal To Apical, Respiratory to paraSternal and transesophageal Cardiac to Respiratory, Aortic to StomacH ultrasonographic evaluations for the assessment of shock. This protocol is better suited for the critically ill patient in its ability to move beyond pattern recognition and focus on monitoring shock states from their presentation through their evolution. Not only is importance placed on the sequence of the exam, but also the identification of signs of chronic disease, the early incorporation of pulmonary evaluation, and the role for transesophageal imaging in critically ill patients with difficult surface imaging. Given the broad capabilities of bedside ultrasound, the Subcostal To Apical, Respiratory to paraSternal-Cardiac to Respiratory, Aortic to StomacH protocol serves as a multifaceted algorithm allowing for a nuanced and dynamic approach for the resuscitation of critically ill patients in shock.
MeSH term(s)	Humans ; Critical Illness ; Heart/diagnostic imaging ; Echocardiography/methods ; Ultrasonography ; Stomach
Language	English
Publishing date	2023-12-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1353113-x
ISSN	1558-5034 ; 0887-2171
ISSN (online)	1558-5034
ISSN	0887-2171
DOI	10.1053/j.sult.2023.12.008
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