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  1. Article ; Online: Video Head Impulse Test Demonstrates a Residual Function after Plugging of Dehiscent Superior Semicircular Canal.

    Renteria, Axel E / Elblidi, Ahlem / Altamami, Nasser / Alhabib, Salman / Saliba, Issam

    Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology

    2023  Volume 44, Issue 3, Page(s) 252–259

    Abstract: Objective: Plugging a symptomatic dehiscent superior semicircular canal (SSCC) often leads to a nonfunctional postoperative canal. However, in some instances, a residual function has been described. This study attempts to describe what factors may lead ... ...

    Abstract Objective: Plugging a symptomatic dehiscent superior semicircular canal (SSCC) often leads to a nonfunctional postoperative canal. However, in some instances, a residual function has been described. This study attempts to describe what factors may lead to such residual function.
    Study design: Retrospective study.
    Setting: Tertiary referral center.
    Patients: Thirty-five patients with confirmed SSCC dehiscence.
    Intervention: Video head impulse test was conducted pre- and postoperatively to assess any difference in the function of the SSCC.
    Main outcome measures: Mean gain and pathological saccades were recorded according to well-established thresholds along with dehiscence length and location to evaluate any associations to residual canal function.
    Results: When comparing preoperative to postoperative SSCC abnormal gains, a significant increase was observed after plugging ( p = 0.023). This also held true when abnormal gain and pathologic saccades were taken together ( p < 0.001). Interestingly, 55.3% of patients were observed to remain with a residual SSCC function 4 months postoperatively even with a clinical improvement. Of these, 47.6% had normal gain with pathologic saccades, 38.1% had an abnormal gain without pathologic saccades, and 14.3% had normal gain without pathologic saccades (normal function). Preoperatively, SSCC abnormal gain was associated with a larger dehiscence length mean ( p = 0.002). Anterosuperior located dehiscences were also associated with a larger dehiscence length mean ( p = 0.037). A residual SSCC function after plugging was associated with a shorter dehiscence length regardless of location ( p = 0.058).
    Conclusion: Dehiscence length and location may be useful in predicting disease symptomatology preoperatively and canals function recovery after plugging. These factors could be used as indicators for preoperative counseling and long-term management.
    MeSH term(s) Humans ; Head Impulse Test ; Retrospective Studies ; Otologic Surgical Procedures ; Semicircular Canals ; Saccades
    Language English
    Publishing date 2023-01-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2036790-9
    ISSN 1537-4505 ; 1531-7129
    ISSN (online) 1537-4505
    ISSN 1531-7129
    DOI 10.1097/MAO.0000000000003794
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Conference proceedings: Outcomes of the Middle Turbinate Swing Technique during the Expanded Endonasal Approach (EEA): A Tertiary Center Experience

    Renteria, Axel E. / Levi, Lirit / Silva, Bruna Castro / Hung, Li-Ting / Azevedo, Alyssa / Lee, Christine K. / Chang, Michael T. / Hwang, Peter H. / Patel, Zara M. / Fernandez-Miranda, Juan-Carlos / Nayak, Jayakar V.

    Journal of Neurological Surgery Part B: Skull Base

    2024  Volume 85, Issue S 01

    Event/congress 33rd Annual Meeting North American Skull Base Society, Atlanta Marriott Marquis Atlanta, Georgia, United States, 2024-02-16
    Language English
    Publishing date 2024-02-01
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 2654269-9
    ISSN 2193-634X ; 2193-6331
    ISSN (online) 2193-634X
    ISSN 2193-6331
    DOI 10.1055/s-0044-1779847
    Database Thieme publisher's database

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  3. Article ; Conference proceedings: Patency of the Cavernous Sinus after Medial Wall Resection: A Tertiary Center Experience

    Renteria, Axel E. / Silva, Bruna Castro / Levi, Lirit / Hung, Li-Ting / Azevedo, Alyssa / Lee, Christine K / Chang, Michael T. / Hwang, Peter H. / Nayak, Jayakar V. / Fischbein, Nancy / Fernandez-Miranda, Juan-Carlos / Patel, Zara M.

    Journal of Neurological Surgery Part B: Skull Base

    2024  Volume 85, Issue S 01

    Event/congress 33rd Annual Meeting North American Skull Base Society, Atlanta Marriott Marquis Atlanta, Georgia, United States, 2024-02-16
    Language English
    Publishing date 2024-02-01
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 2654269-9
    ISSN 2193-634X ; 2193-6331
    ISSN (online) 2193-634X
    ISSN 2193-6331
    DOI 10.1055/s-0044-1779881
    Database Thieme publisher's database

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  4. Article ; Online: Utilization of Transcriptomic Profiling to Identify Molecular Markers Predicting Successful Recovery Following Endoscopic Sinus Surgery for Chronic Rhinosinusitis.

    Renteria, Axel E / Maniakas, Anastasios / Pelletier, Audrey / Filali-Mouhim, Ali / Brochiero, Emmanuelle / Valera, Fabiana C P / Adam, Damien / Mfuna, Leandra Endam / Desrosiers, Martin

    Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

    2023  Volume 169, Issue 6, Page(s) 1662–1673

    Abstract: Objectives: Successful recovery from chronic rhinosinusitis (CRS) following endoscopic sinus surgery (ESS) can be characterized by minimal presence of symptoms and absence of disease on endoscopy. However, molecular markers of surgical success remain to ...

    Abstract Objectives: Successful recovery from chronic rhinosinusitis (CRS) following endoscopic sinus surgery (ESS) can be characterized by minimal presence of symptoms and absence of disease on endoscopy. However, molecular markers of surgical success remain to be characterized. These could allow for better tailoring of perioperative therapy. This study aims to identify novel molecular markers associated with surgery responsive patient.
    Study design: Prospective cohort study.
    Setting: Single academic hospital center.
    Method: One hundred eighteen consecutive patients with CRS at high risk of recurrence after surgery were followed prospectively following ESS in an academic medical center. Symptomatic and endoscopic outcomes were assessed at 4 months, with success rigorously defined subjectively as minimal or no symptoms (no symptom greater than 1 on an ordinal scale of 0-3) and objectively by the absence of nasal polyposis on sinus cavity endoscopy and Lund-Kennedy endoscopic edema score no greater than 1. Samples were obtained at the time of surgery and at 4-month postoperatively. Changes associated with surgery were determined by gene expression profiling using Affymetrix's Clariom S Human HT arrays.
    Results: Successful ESS was characterized by a mild upregulation in Type 1 inflammation, upregulation of cell cycle progression, and epithelial barrier and proliferation-associated genes and pathways. ESS failure was associated to very high levels of Type 1 inflammation along with downregulation of epithelial barrier function and regeneration genes and pathways.
    Conclusion: Successful recovery from ESS involves restoration of epithelial function and regulated activation of Type 1 inflammation. Excessively elevated Type 1 inflammation is associated with epithelial barrier dysfunction.
    MeSH term(s) Humans ; Prospective Studies ; Transcriptome ; Rhinitis/genetics ; Rhinitis/surgery ; Rhinitis/complications ; Sinusitis/genetics ; Sinusitis/surgery ; Sinusitis/complications ; Inflammation/complications ; Nasal Polyps/genetics ; Nasal Polyps/surgery ; Nasal Polyps/complications ; Biomarkers ; Endoscopy ; Chronic Disease ; Gene Expression Profiling ; Treatment Outcome
    Chemical Substances Biomarkers
    Language English
    Publishing date 2023-08-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 392085-9
    ISSN 1097-6817 ; 0161-6439 ; 0194-5998
    ISSN (online) 1097-6817
    ISSN 0161-6439 ; 0194-5998
    DOI 10.1002/ohn.482
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Azithromycin Mechanisms of Action in CRS Include Epithelial Barrier Restoration and Type 1 Inflammation Reduction.

    Renteria, Axel E / Valera, Fabiana C P / Maniakas, Anastasios / Adam, Damien / Filali-Mouhim, Ali / Ruffin, Manon / Mfuna, Leandra Endam / Brochiero, Emmanuelle / Desrosiers, Martin Y

    Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

    2023  Volume 169, Issue 4, Page(s) 1055–1063

    Abstract: Objective: Previous in vitro transcriptomic profiling suggests azithromycin exerts its effects in patients with chronic rhinosinusitis (CRS) via modulation of type 1 inflammation and restoration of epithelial barrier function. We wished to verify these ... ...

    Abstract Objective: Previous in vitro transcriptomic profiling suggests azithromycin exerts its effects in patients with chronic rhinosinusitis (CRS) via modulation of type 1 inflammation and restoration of epithelial barrier function. We wished to verify these postulated effects using in vitro models of epithelial repair and in vivo transcriptional profiling.
    Study design: Functional effects of azithromycin in CRS were verified using in vitro models of wounding. The mechanism of the effect of azithromycin was assessed in vivo using transcriptomic profiling.
    Setting: Academic medical center.
    Methods: Effects of azithromycin on the speed of epithelial repair were verified in a wounding model using primary nasal epithelial cells (pNEC) from CRS patients. Nasal brushings collected pre-and posttreatment during a placebo-controlled trial of azithromycin for CRS patients unresponsive to surgery underwent transcriptomic profiling to identify implicated pathways.
    Results: Administration of azithromycin improved the wound healing rates in CRS pNECs and prevented the negative effect of Staphylococcus aureus on epithelial repair. In vivo, response to azithromycin was associated with downregulation in pathways of type 1 inflammation, and upregulation of pathways implicated in the restoration of the cell cycle.
    Conclusion: Restoration of healthy epithelial function may represent a major mode of action of azithromycin in CRS. In vitro models show enhanced epithelial repair, while in vivo transcriptomics shows downregulation of pathways type 1 inflammation accompanied by upregulation of DNA repair and cell-cycle pathways. The maximal effect in patients with high levels of type 1-enhanced inflammation suggests that azithromycin may represent a novel therapeutic option for surgery-unresponsive CRS patients.
    MeSH term(s) Humans ; Azithromycin/pharmacology ; Azithromycin/therapeutic use ; Azithromycin/metabolism ; Rhinitis/complications ; Nasal Polyps/complications ; Sinusitis/complications ; Inflammation/drug therapy ; Inflammation/complications ; Chronic Disease ; Nasal Mucosa/pathology
    Chemical Substances Azithromycin (83905-01-5)
    Language English
    Publishing date 2023-05-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 392085-9
    ISSN 1097-6817 ; 0161-6439 ; 0194-5998
    ISSN (online) 1097-6817
    ISSN 0161-6439 ; 0194-5998
    DOI 10.1002/ohn.355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Intranasal Application of

    Endam, Leandra Mfuna / Alromaih, Saud / Gonzalez, Emmanuel / Madrenas, Joaquin / Cousineau, Benoit / Renteria, Axel E / Desrosiers, Martin

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 440

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Carnobacteriaceae ; Chronic Disease ; Humans ; Lactococcus lactis ; Prospective Studies ; Quality of Life ; Rhinitis/therapy
    Language English
    Publishing date 2020-10-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.00440
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Low-dose and long-term azithromycin significantly decreases Staphylococcus aureus in the microbiome of refractory CRS patients.

    Renteria, Axel E / Maniakas, Anastasios / Mfuna, Leandra Endam / Asmar, Marc-Henri / Gonzalez, Emmanuel / Desrosiers, Martin

    International forum of allergy & rhinology

    2020  Volume 11, Issue 2, Page(s) 93–105

    Abstract: Background: The sinonasal microbiome is believed to play an important role in the pathophysiology of refractory chronic rhinosinusitis (CRS). We evaluated changes in the microbiome following a 4-month course of low-dose azithromycin. Assessing ... ...

    Abstract Background: The sinonasal microbiome is believed to play an important role in the pathophysiology of refractory chronic rhinosinusitis (CRS). We evaluated changes in the microbiome following a 4-month course of low-dose azithromycin. Assessing microbiome alterations following such a treatment may help identify underlying mechanisms of this drug.
    Methods: A total of 48 adults with refractory CRS were enrolled in a double-blind, randomized, placebo-controlled trial. Patients were randomized to 250 mg of azithromycin or placebo 3 times weekly for 4 months. During this time, daily budesonide saline irrigations were continued. Sinonasal swabs were collected by endoscopically-assisted method prior to treatment initiation and at the end of it, and sent for 16S ribosomal RNA gene sequencing. High-resolution ANCHOR pipeline was used to infer and annotate putative species. The 2 patient groups were compared using DESeq2 differential abundance analysis.
    Results: From initiation to the end of azithromycin treatment, patients showed a significant difference in beta diversity analysis (p = 0.0004) along with a significant decrease in 71 different operational taxonomic units (OTUs) of Staphylococcus aureus (false discovery rate [FDR] < 0.05) obtained from the differential abundance analysis. This was not observed in placebo-treated patients. By the end of treatments, azithromycin-treated patients had a significant decrease in 29 different OTUs of S. aureus (FDR < 0.05) when compared to placebo.
    Conclusion: A 4-month course of 250 mg of azithromycin 3 times weekly in patients with refractory CRS significantly decreases S. aureus abundance in the sinonasal microbiome. Considering the pathogenic role of S. aureus in the refractory CRS population, azithromycin may constitute an additional therapeutic option to help control this disease.
    MeSH term(s) Adult ; Azithromycin/therapeutic use ; Chronic Disease ; Humans ; Microbiota ; Rhinitis/drug therapy ; Sinusitis/drug therapy ; Staphylococcus aureus
    Chemical Substances Azithromycin (83905-01-5)
    Language English
    Publishing date 2020-07-26
    Publishing country United States
    Document type Journal Article ; Randomized Controlled Trial
    ZDB-ID 2625826-2
    ISSN 2042-6984 ; 2042-6976
    ISSN (online) 2042-6984
    ISSN 2042-6976
    DOI 10.1002/alr.22653
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Do Aging Factors Influence the Clinical Presentation and Management of Chronic Rhinosinusitis?

    Renteria, Axel E / Mfuna Endam, Leandra / Desrosiers, Martin

    Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

    2017  Volume 156, Issue 4, Page(s) 598–605

    Abstract: Objective/Hypothesis Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the upper respiratory airways resulting from the dysregulation of immunity and epithelial defenses. More recently, the contribution of an altered nasal microbiome to ... ...

    Abstract Objective/Hypothesis Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the upper respiratory airways resulting from the dysregulation of immunity and epithelial defenses. More recently, the contribution of an altered nasal microbiome to the development of CRS has also been proposed. However, the impact of aging on the development of CRS has been long overlooked. Here we propose, in a hypothesis piece, that aging can influence the physiopathology of CRS and its subsequent management in an elderly population. Data Sources We summarize the recent literature findings supporting that elderly patients with CRS could be a distinct population from those with adult CRS and might require different or adjunct therapeutic approaches. Methods Review of recent literature of the effect of aging and its possible effects in CRS using 3 different databases. Conclusions Age-dependent decrease in the levels of the S100 family proteins involved in epithelial proliferation, repair, and defenses combined with chronic inflammation might lead to an increased risk of abnormal microbial colonization and loss of microbiota diversity. Ultimately, these changes could have the potential to alter the physiopathology of CRS in the elderly. Implications Unlike in adults, in whom CRS T
    MeSH term(s) Age Factors ; Aged ; Aging/immunology ; Aging/physiology ; Humans ; Microbiota ; Nasal Mucosa/physiology ; Rhinitis/physiopathology ; Rhinitis/therapy ; S100 Proteins/physiology ; Sinusitis/physiopathology ; Sinusitis/therapy
    Chemical Substances S100 Proteins
    Language English
    Publishing date 2017-04
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 392085-9
    ISSN 1097-6817 ; 0161-6439 ; 0194-5998
    ISSN (online) 1097-6817
    ISSN 0161-6439 ; 0194-5998
    DOI 10.1177/0194599817691258
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining.

    Sonzogni-Desautels, Karine / Di Lenardo, Thomas Z / Renteria, Axel E / Gascon, Marc-André / Geary, Timothy G / Ndao, Momar

    PLoS neglected tropical diseases

    2019  Volume 13, Issue 3, Page(s) e0007259

    Abstract: Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens the lives of young children in developing countries. In veterinary medicine, C. parvum causes life-threatening diarrhea and dehydration in newborn dairy ... ...

    Abstract Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens the lives of young children in developing countries. In veterinary medicine, C. parvum causes life-threatening diarrhea and dehydration in newborn dairy calves. Protocols to detect Cryptosporidium spp. oocysts using flow cytometry have been reported; however, these protocols use antibodies against the parasite and typically focus on detection of oocysts, not quantification. These techniques are not well-suited for studies that generate large variations in oocyst burdens because the amount of antibody required is proportional to the number of oocysts expected in samples. Also, oocysts are lost in washes in the staining protocol, reducing accuracy of oocyst counts. Moreover, these protocols require costly fluorochrome-conjugated monoclonal antibodies and are not optimal for studies involving large numbers of samples. Here we present an optimized protocol for purifying oocysts from mouse stool and intestine samples combined with a reliable method to quantify oocysts in a relatively pure population without the need for antibody staining. We used morphology (SSC-A vs FSC-A) and the innate characteristics of C. parvum oocysts compared to fecal and intestinal contaminants to develop a two-step gating strategy that can differentiate oocysts from debris. This method is a fast, reliable, and high-throughput technique to promote research projects on C. parvum infections in mice and potentially other animal hosts.
    MeSH term(s) Animals ; Cryptosporidiosis/parasitology ; Cryptosporidium/isolation & purification ; Disease Models, Animal ; Feces/parasitology ; Flow Cytometry/methods ; Mice, Inbred C57BL ; Oocysts/isolation & purification ; Parasite Load/methods
    Language English
    Publishing date 2019-03-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2727
    ISSN (online) 1935-2735
    ISSN 1935-2727
    DOI 10.1371/journal.pntd.0007259
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Staphylococcus aureus

    Maniakas, Anastasios / Asmar, Marc-Henri / Renteria Flores, Axel E / Nayan, Smriti / Alromaih, Saud / Mfuna Endam, Leandra / Desrosiers, Martin Y

    Frontiers in cellular and infection microbiology

    2018  Volume 8, Page(s) 150

    Abstract: Objectives: ...

    Abstract Objectives:
    MeSH term(s) Adolescent ; Adult ; Aged ; Budesonide ; Child ; Chronic Disease ; Endoscopy/adverse effects ; Female ; Humans ; Male ; Middle Aged ; Paranasal Sinuses/microbiology ; Paranasal Sinuses/surgery ; Prospective Studies ; Recurrence ; Reoperation/adverse effects ; Sinusitis/microbiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/pathogenicity ; Tertiary Care Centers ; Young Adult
    Chemical Substances Budesonide (51333-22-3)
    Language English
    Publishing date 2018-05-15
    Publishing country Switzerland
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2018.00150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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