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  1. Book: Mammary gland development

    Martin, Finian / Stein, Torsten / Howlin, Jillian

    methods and protocols

    (Methods in molecular biology ; 1501 ; Springer protocols)

    2017  

    Author's details edited by Finian Martin, Torsten Stein, Jillian Howlin
    Series title Methods in molecular biology ; 1501
    Springer protocols
    Collection
    Keywords 2D and 3D-model culture systems ; genetically modified mouse models ; human breast cancer ; mammary stem cell isolation ; pubertal mammary gland phenotypes ; stem cells
    Language English
    Size xi, 358 Seiten, Illustrationen, Diagramme, 25.4 cm x 17.8 cm, 0 g
    Publisher Humana Press
    Publishing place New York
    Publishing country United States
    Document type Book
    HBZ-ID HT019124234
    ISBN 978-1-4939-6473-4 ; 1-4939-6473-9 ; 9781493964758 ; 1493964755
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    Zs A 7042 -1501- verfügbar in Königswinter Königswinter

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  2. Article ; Online: Oxygen-Limiting Growth Conditions and Deletion of the Transition State Regulator Protein Abrb in Bacillus subtilis 6633 Result in an Increase in Subtilosin Production and a Decrease in Subtilin Production.

    Stein, Torsten

    Probiotics and antimicrobial proteins

    2019  Volume 12, Issue 2, Page(s) 725–731

    Abstract: It has been recently shown, that certain strains/isolates of Bacillus subtilis can be used as a probiotic for humans. The production of the macrocyclic sactibiotic subtilosin in B. subtilis ATCC 6633 is highly regulated. To improve the subtilosin ... ...

    Abstract It has been recently shown, that certain strains/isolates of Bacillus subtilis can be used as a probiotic for humans. The production of the macrocyclic sactibiotic subtilosin in B. subtilis ATCC 6633 is highly regulated. To improve the subtilosin productivity of B. subtilis, different growth conditions were compared for maximal expression of the sbo promoter that regulates the expression of the subtilosin biosynthetic gene cluster. Oxygen-limiting conditions led to a strong increase of sbo promoter activities compared to aerobic conditions, and accordingly, the subtilosin amount determined by reversed phase HPLC (7.8 mg/L) was 15-fold superior to the amount of aerobic grown cultures (0.5 mg/L). A further promising enhancement of the subtilosin yield was achieved using a deletion mutant that is avoiding the general transition state regulator protein AbrB. The subtilosin titer of 42 mg/L produced by ΔabrB cells grown under oxygen-limiting conditions corresponds to an 84-fold increase compared to the subtilosin titer obtained from B. subtilis wild type cells propagated in aerobic conditions. Furthermore, evidence is provided that oxygen-limiting conditions led to a strong decrease in the productivity of the lantipeptide subtilin suggesting contrary regulatory mechanisms for the B. subtilis antimicrobials subtilin and subtilosin.
    MeSH term(s) Anti-Bacterial Agents/biosynthesis ; Bacillus subtilis/genetics ; Bacillus subtilis/growth & development ; Bacillus subtilis/metabolism ; Bacteriocins/biosynthesis ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Oxygen/metabolism ; Peptides, Cyclic/biosynthesis
    Chemical Substances Anti-Bacterial Agents ; Bacteriocins ; Peptides, Cyclic ; sboA protein, Bacillus subtilis (98914-01-3) ; subtilin (JCA69WH3TT) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2019-04-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2487792-X
    ISSN 1867-1314 ; 1867-1306
    ISSN (online) 1867-1314
    ISSN 1867-1306
    DOI 10.1007/s12602-019-09547-4
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  3. Article ; Online: High-resolution mapping of Ryd4

    Pidon, Hélène / Ruge-Wehling, Brigitte / Will, Torsten / Habekuß, Antje / Wendler, Neele / Oldach, Klaus / Maasberg-Prelle, Anja / Korzun, Viktor / Stein, Nils

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    2024  Volume 137, Issue 3, Page(s) 60

    Abstract: Key message: We mapped ... ...

    Abstract Key message: We mapped Ryd4
    MeSH term(s) Chromosome Mapping ; Hordeum/genetics ; Genetic Markers ; Disease Resistance/genetics ; Luteovirus/genetics ; Plant Breeding ; Plant Diseases
    Chemical Substances Genetic Markers
    Language English
    Publishing date 2024-02-27
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    DOI 10.1007/s00122-024-04542-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Umweltsituation und Umweltbewegung in Estland

    Stein, Torsten

    (Arbeitspapiere des Instituts für Internationale Politik und Regionalstudien ; Nr. 10)

    1995  

    Author's details Torsten Stein. Institut für Internationale Politik und Regionalstudien des Fachbereichs Politische Wissenschaft der Freien Universität Berlin
    Series title Arbeitspapiere des Instituts für Internationale Politik und Regionalstudien ; Nr. 10
    Collection
    Keywords Estland ; Natürliche Ressourcen ; Ressourcenallokation ; Umweltschaden ; Umweltschutz
    Subject Ökologie ; Umweltvorsorge ; Umweltzerstörung ; Zivilisationsschaden ; Ökologischer Schaden ; Ressourcen ; Naturgüter ; Naturschätze ; Naturressourcen ; Natürliche Ressource
    Size 20 Bl. : graph. Darst.
    Publisher Inst. für Internat. Politik und Regionalstudien
    Publishing place Berlin
    Document type Book
    HBZ-ID HT011142765
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Shelf markLoan statusLocation
    994/1924 available for loan (reading room) Freihandmagazin (U1)

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  5. Article ; Online: Antibiotic profiling of wild-type bacilli led to the discovery of new lanthipeptide subtilin-producing Bacillus spizizenii strains whose 16S rDNA sequences differ from the B. spizizenii typing strain.

    Helfrich, Markus / Entian, Karl-Dieter / Stein, Torsten

    International microbiology : the official journal of the Spanish Society for Microbiology

    2022  Volume 25, Issue 4, Page(s) 839–850

    Abstract: Two dozen field-collected Bacillus and a dozen Bacillus spizizenii wild-type strains from strain collections were selected on the basis of their antagonistic properties against the Gram-positive strain Micrococcus luteus. Based on their genetic and ... ...

    Abstract Two dozen field-collected Bacillus and a dozen Bacillus spizizenii wild-type strains from strain collections were selected on the basis of their antagonistic properties against the Gram-positive strain Micrococcus luteus. Based on their genetic and antibiotic profiles, they were characterized (subtilin encoding spaS gene sequences, mass spectrometric, and quantitative-reversed phase liquid chromatographic analyses, as well as the presence of the lanthionine cyclase protein SpaC by western blotting), seven novel producers of the lanthipeptide subtilin. Phylogenetic analyses of the subtilin-producing wild-type strains based on their 16S rRNA sequences showed that all seven strains could be classified as B. spizizenii: The field-collected strains HS and N5, as well as strains DSM 618, 1087, 6395, 6405, and 8439 from the German Collection of Microorganisms and Cell Cultures. To the best of our knowledge, all B. spizizenii strains described so far are characterized by the fact that they can produce a lanthipeptide of the subtilin family. Both the lanthipeptide structures and the organization and sequences of the 16S rRNA-encoding genes suggest a subdivision of B. spizizenii into subspecies: The subtilin-producing B. spizizenii strains are distinctly different from the entianin-producing B. spizizenii typing strain TU-B-10
    MeSH term(s) Anti-Bacterial Agents/metabolism ; Bacillus/genetics ; Bacillus subtilis/chemistry ; Bacteriocins ; DNA, Bacterial/genetics ; DNA, Ribosomal/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics
    Chemical Substances Anti-Bacterial Agents ; Bacteriocins ; DNA, Bacterial ; DNA, Ribosomal ; RNA, Ribosomal, 16S ; subtilin (JCA69WH3TT)
    Language English
    Publishing date 2022-07-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1454951-7
    ISSN 1618-1905 ; 1139-6709
    ISSN (online) 1618-1905
    ISSN 1139-6709
    DOI 10.1007/s10123-022-00266-5
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4964: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  6. Book ; Thesis: Hämodilution bei akuter zerebraler Ischämie

    Stein, Torsten

    Vergleich der Wirkungen von vier Infusionslösungen auf hämorheologische und hämodynamische Parameter sowie den konjunktivalen Sauerstoffpartialdruck

    1992  

    Author's details vorgelegt von Torsten Stein
    Size 119 Bl. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Heidelberg, Univ., Diss., 1992
    HBZ-ID HT004966659
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    93 E 2163 order for loan Magazin

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  7. Article ; Online: SFRP1 Expression is Inversely Associated With Metastasis Formation in Canine Mammary Tumours.

    Seitz, Judith / Bilsland, Alan / Puget, Chloé / Baasner, Ian / Klopfleisch, Robert / Stein, Torsten

    Journal of mammary gland biology and neoplasia

    2023  Volume 28, Issue 1, Page(s) 15

    Abstract: Background: Canine mammary tumours (CMTs) are the most frequent tumours in intact female dogs and show strong similarities with human breast cancer. In contrast to the human disease there are no standardised diagnostic or prognostic biomarkers available ...

    Abstract Background: Canine mammary tumours (CMTs) are the most frequent tumours in intact female dogs and show strong similarities with human breast cancer. In contrast to the human disease there are no standardised diagnostic or prognostic biomarkers available to guide treatment. We recently identified a prognostic 18-gene RNA signature that could stratify human breast cancer patients into groups with significantly different risk of distant metastasis formation. Here, we assessed whether expression patterns of these RNAs were also associated with canine tumour progression.
    Method: A sequential forward feature selection process was performed on a previously published microarray dataset of 27 CMTs with and without lymph node (LN) metastases to identify RNAs with significantly differential expression to identify prognostic genes within the 18-gene signature. Using an independent set of 33 newly identified archival CMTs, we compared expression of the identified prognostic subset on RNA and protein basis using RT-qPCR and immunohistochemistry on FFPE-tissue sections.
    Results: While the 18-gene signature as a whole did not have any prognostic power, a subset of three RNAs: Col13a1, Spock2, and Sfrp1, together completely separated CMTs with and without LN metastasis in the microarray set. However, in the new independent set assessed by RT-qPCR, only the Wnt-antagonist Sfrp1 showed significantly increased mRNA abundance in CMTs without LN metastases on its own (p = 0.013) in logistic regression analysis. This correlated with stronger SFRP1 protein staining intensity of the myoepithelium and/or stroma (p < 0.001). SFRP1 staining, as well as β-catenin membrane staining, was significantly associated with negative LN status (p = 0.010 and 0.014 respectively). However, SFRP1 did not correlate with β-catenin membrane staining (p = 0.14).
    Conclusion: The study identified SFRP1 as a potential biomarker for metastasis formation in CMTs, but lack of SFRP1 was not associated with reduced membrane-localisation of β-catenin in CMTs.
    MeSH term(s) Humans ; Dogs ; Animals ; Female ; beta Catenin/metabolism ; Prognosis ; Lymphatic Metastasis ; Mammary Neoplasms, Animal/pathology ; RNA ; Breast Neoplasms/genetics
    Chemical Substances beta Catenin ; RNA (63231-63-0)
    Language English
    Publishing date 2023-07-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1327345-0
    ISSN 1573-7039 ; 1083-3021
    ISSN (online) 1573-7039
    ISSN 1083-3021
    DOI 10.1007/s10911-023-09543-z
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    Zs.A 4478: Show issues Location:
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  8. Article ; Online: CONSTITUTIONAL SOCIO-ECONOMIC RIGHTS AND INTERNATIONAL LAW

    Torsten Stein

    Potchefstroom Electronic Law Journal, Vol 16, Iss 1, Pp 12-

    "YOU ARE NOT ALONE"

    2013  Volume 30

    Abstract: The second oratio was a keynote speech delivered by Torsten Stein, the Director of the Institute ...

    Abstract The second oratio was a keynote speech delivered by Torsten Stein, the Director of the Institute of European Studies and holder of the chair for European law and European Public Law at Saarland University, Germany. He delivered his speech during November 2012 at the 3rd Human Rights Indaba on The Role of International Law in Understanding and Applying the Socio-economic Rights in South Africa's Bill of Rights, which was held by the Faculty of Law (NWU, Potchefstroom Campus) in collaboration with the Konrad-Adenauer Foundation. He shared some thoughts about the nature, development and implementation of socio-economic rights within an international and European setting.
    Keywords Law in general. Comparative and uniform law. Jurisprudence ; K1-7720 ; Law ; K ; DOAJ:Law ; DOAJ:Law and Political Science
    Language Afrikaans
    Publishing date 2013-04-01T00:00:00Z
    Publisher North-West University
    Document type Article ; Online
    DOI 10.4314/pelj.v16i1.2
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Book ; Thesis: Thema: Nosokomiale Infektionen bei Früh- und Neugeborenen der Neonatologischen Intensivstation des Klinikums Frankfurt (Oder) 1989 - 1992

    Stein, Sabine / Karsch, Torsten

    1994  

    Author's details vorgelegt von Sabine Stein und Torsten Karsch
    Language German
    Size 112, 2 Bl. : graph. Darst.
    Edition [Mikrofiche-Ausg.]
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Berlin, Humboldt-Univ., Diss., 1994
    Note Mikrofiche-Ausg.: 2 Mikrofiches : 24x
    HBZ-ID HT006780403
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    1995 MB 2258 order for loan Magazin

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  10. Book: Herausforderungen an Staat und Verfassung

    Calliess, Christian / Stein, Torsten

    Völkerrecht - Europarecht - Menschenrechte ; Liber Amicorum für Torsten Stein zum 70. Geburtstag

    (Schriften des Europa-Instituts der Universität des Saarlandes - Rechtswissenschaft ; 100)

    2015  

    Author's details Christian Calliess (Hrsg.)
    Series title Schriften des Europa-Instituts der Universität des Saarlandes - Rechtswissenschaft ; 100
    Language German ; English ; French
    Size 1088 S., Ill.
    Edition 1. Aufl.
    Publisher Nomos
    Publishing place Baden-Baden
    Document type Book
    Note Beitr. teilw. dt., teilw. engl., teilw. franz.
    ISBN 9783848713479 ; 3848713470
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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