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  1. Article ; Online: Root volatiles manipulate bacterial biofilms.

    Raza, Waseem / Jiang, Gaofei

    Nature ecology & evolution

    2024  

    Language English
    Publishing date 2024-04-09
    Publishing country England
    Document type Journal Article
    ISSN 2397-334X
    ISSN (online) 2397-334X
    DOI 10.1038/s41559-024-02403-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Protist predation selects for the soil resistome.

    Jiang, Gaofei / Liu, Chen / Xiong, Wu / Shen, Qirong / Wei, Zhong

    The ISME journal

    2024  Volume 18, Issue 1

    Abstract: A key aspect of "One Health" is to comprehend how antibiotic resistomes evolve naturally. In this issue, Nguyen and colleagues pioneered an in situ investigation on the impact of protist predations on the soil microbial community and its antibiotic ... ...

    Abstract A key aspect of "One Health" is to comprehend how antibiotic resistomes evolve naturally. In this issue, Nguyen and colleagues pioneered an in situ investigation on the impact of protist predations on the soil microbial community and its antibiotic resistance genes (ARGs). They found that bacterivorous protists consistently increased the abundance of ARGs, such as tetracycline resistant genes. Indeed, antibiotic production is a common strategy for bacteria to evade protist predation. The rise of ARGs can be explained by the balance between antibiotic producers and resisters shaped by predatory selection. This work suggests that ARG enrichment due to biotic interactions may be less worrisome than previously thought. Unless, these ARGs are carried by or disseminated among pathogens. Therefore, it is essential to monitor the occurrence, dissemination and pathogenic hosts of ARGs, enhancing our capacity to combat antibiotic resistance.
    MeSH term(s) Animals ; Genes, Bacterial ; Soil ; Predatory Behavior ; Soil Microbiology ; Anti-Bacterial Agents/pharmacology
    Chemical Substances Soil ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-02-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2406536-5
    ISSN 1751-7370 ; 1751-7362
    ISSN (online) 1751-7370
    ISSN 1751-7362
    DOI 10.1093/ismejo/wrad007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Engineering Substrate Promiscuity of Nucleoside Phosphorylase Via an Insertions-Deletions Strategy.

    Liu, Gaofei / Wang, Jialing / Chu, Jianlin / Jiang, Tianyue / Qin, Song / Gao, Zhen / He, Bingfang

    JACS Au

    2024  Volume 4, Issue 2, Page(s) 454–464

    Abstract: Nucleoside phosphorylases (NPs) are the key enzymes in the nucleoside metabolism pathway and are widely employed for the synthesis of nucleoside analogs, which are difficult to access via conventional synthetic methods. NPs are generally classified as ... ...

    Abstract Nucleoside phosphorylases (NPs) are the key enzymes in the nucleoside metabolism pathway and are widely employed for the synthesis of nucleoside analogs, which are difficult to access via conventional synthetic methods. NPs are generally classified as purine nucleoside phosphorylase (PNP) and pyrimidine or uridine nucleoside phosphorylase (PyNP/UP), based on their substrate preference. Here, based on the evolutionary information on the NP-I family, we adopted an insertions-deletions (InDels) strategy to engineer the substrate promiscuity of nucleoside phosphorylase
    Language English
    Publishing date 2024-01-13
    Publishing country United States
    Document type Journal Article
    ISSN 2691-3704
    ISSN (online) 2691-3704
    DOI 10.1021/jacsau.3c00581
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microbe-induced phenotypic variation leads to overyielding in clonal plant populations.

    Raza, Waseem / Jiang, Gaofei / Eisenhauer, Nico / Huang, Yishuo / Wei, Zhong / Shen, Qirong / Kowalchuk, George A / Jousset, Alexandre

    Nature ecology & evolution

    2024  Volume 8, Issue 3, Page(s) 392–399

    Abstract: Overyielding, the high productivity of multispecies plant communities, is commonly seen as the result of plant genetic diversity. Here we demonstrate that biodiversity-ecosystem functioning relationships can emerge in clonal plant populations through ... ...

    Abstract Overyielding, the high productivity of multispecies plant communities, is commonly seen as the result of plant genetic diversity. Here we demonstrate that biodiversity-ecosystem functioning relationships can emerge in clonal plant populations through interaction with microorganisms. Using a model clonal plant species, we found that exposure to volatiles of certain microorganisms led to divergent plant phenotypes. Assembling communities out of plants associated with different microorganisms led to transgressive overyielding in both biomass and seed yield. Our results highlight the importance of belowground microbial diversity in plant biodiversity research and open new avenues for precision ecosystem management.
    MeSH term(s) Ecosystem ; Biodiversity ; Biomass ; Plants ; Population Dynamics
    Language English
    Publishing date 2024-01-09
    Publishing country England
    Document type Journal Article
    ISSN 2397-334X
    ISSN (online) 2397-334X
    DOI 10.1038/s41559-023-02297-1
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  5. Article: Improved water solubility, chemical stability, antioxidant and anticancer activity of resveratrol via nanoencapsulation with pea protein nanofibrils

    Yi, Jiang / He, Qingyu / Peng, Gaofei / Fan, Yuting

    Food chemistry. 2022 May 30, v. 377

    2022  

    Abstract: Fabricated pea protein isolate (PPI) nanofibrils were used as nanocarriers to encapsulate, stabilize and deliver resveratrol (RES). PPI nanofibrils possessed a dramatically higher surface hydrophobicity than PPI (native), and PPI nanofibrils exhibited ... ...

    Abstract Fabricated pea protein isolate (PPI) nanofibrils were used as nanocarriers to encapsulate, stabilize and deliver resveratrol (RES). PPI nanofibrils possessed a dramatically higher surface hydrophobicity than PPI (native), and PPI nanofibrils exhibited nanoscale widths of 10 nm and average lengths of 1.0 μm. Fluorescence analyses demonstrated PPI nanofibrils had high binding constant with RES. Compared with RES (free), the aqueous solubility of RES was improved by approximately 1000-fold with PPI nanofibrils complex. DPPH and ABTS radical scavenging activity assays showed that the antioxidant capacity of RES was pronouncedly enhanced through the nanocomplexation with PPI nanofibrils. RES-PPI nanofibrils complexes exhibited higher antiproliferative activities than RES (free), with the cell viabilities of 52.6% and 38.5% for RES (free) and RES-PPI nanofibrils complex at 20 μg/mL. This study demonstrates that PPI nanofibrils can be utilized as novel nanocarriers for improvements of the water solubility, chemical stability and in vitro biological activities of hydrophobic nutraceuticals.
    Keywords antineoplastic activity ; antioxidant activity ; antioxidants ; dietary supplements ; fluorescence ; food chemistry ; hydrophobicity ; nanocapsules ; nanocarriers ; nanofibers ; pea protein ; protein isolates ; resveratrol ; water solubility
    Language English
    Dates of publication 2022-0530
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2021.131942
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Deep-Learning-Enabled Microwave-Induced Thermoacoustic Tomography Based on ResAttU-Net for Transcranial Brain Hemorrhage Detection.

    Li, Chenzhe / Xi, Zijun / Jin, Gaofei / Jiang, Weichao / Wang, Baosheng / Cai, Xiran / Wang, Xiong

    IEEE transactions on bio-medical engineering

    2023  Volume 70, Issue 8, Page(s) 2350–2361

    Abstract: Objective: Hemorrhagic stroke is a leading threat to human's health. The fast-developing microwave-induced thermoacoustic tomography (MITAT) technique holds potential to do brain imaging. However, transcranial brain imaging based on MITAT is still ... ...

    Abstract Objective: Hemorrhagic stroke is a leading threat to human's health. The fast-developing microwave-induced thermoacoustic tomography (MITAT) technique holds potential to do brain imaging. However, transcranial brain imaging based on MITAT is still challenging due to the involved huge heterogeneity in speed of sound and acoustic attenuation of human skull. This work aims to address the adverse effect of the acoustic heterogeneity using a deep-learning-based MITAT (DL-MITAT) approach for transcranial brain hemorrhage detection.
    Methods: We establish a new network structure, a residual attention U-Net (ResAttU-Net), for the proposed DL-MITAT technique, which exhibits improved performance as compared to some traditionally used networks. We use simulation method to build training sets and take images obtained by traditional imaging algorithms as the input of the network.
    Results: We present ex-vivo transcranial brain hemorrhage detection as a proof-of-concept validation. By using an 8.1-mm thick bovine skull and porcine brain tissues to perform ex-vivo experiments, we demonstrate that the trained ResAttU-Net is capable of efficiently eliminating image artifacts and accurately restoring the hemorrhage spot. It is proved that the DL-MITAT method can reliably suppress false positive rate and detect a hemorrhage spot as small as 3 mm. We also study effects of several factors of the DL-MITAT technique to further reveal its robustness and limitations.
    Conclusion: The proposed ResAttU-Net-based DL-MITAT method is promising for mitigating the acoustic inhomogeneity issue and performing transcranial brain hemorrhage detection.
    Significance: This work provides a novel ResAttU-Net-based DL-MITAT paradigm and paves a compelling route for transcranial brain hemorrhage detection as well as other transcranial brain imaging applications.
    MeSH term(s) Animals ; Cattle ; Humans ; Swine ; Deep Learning ; Microwaves ; Image Processing, Computer-Assisted/methods ; Tomography, X-Ray Computed ; Intracranial Hemorrhages/diagnostic imaging
    Language English
    Publishing date 2023-07-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 160429-6
    ISSN 1558-2531 ; 0018-9294
    ISSN (online) 1558-2531
    ISSN 0018-9294
    DOI 10.1109/TBME.2023.3243491
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Improved water solubility, chemical stability, antioxidant and anticancer activity of resveratrol via nanoencapsulation with pea protein nanofibrils.

    Yi, Jiang / He, Qingyu / Peng, Gaofei / Fan, Yuting

    Food chemistry

    2021  Volume 377, Page(s) 131942

    Abstract: Fabricated pea protein isolate (PPI) nanofibrils were used as nanocarriers to encapsulate, stabilize and deliver resveratrol (RES). PPI nanofibrils possessed a dramatically higher surface hydrophobicity than PPI (native), and PPI nanofibrils exhibited ... ...

    Abstract Fabricated pea protein isolate (PPI) nanofibrils were used as nanocarriers to encapsulate, stabilize and deliver resveratrol (RES). PPI nanofibrils possessed a dramatically higher surface hydrophobicity than PPI (native), and PPI nanofibrils exhibited nanoscale widths of 10 nm and average lengths of 1.0 μm. Fluorescence analyses demonstrated PPI nanofibrils had high binding constant with RES. Compared with RES (free), the aqueous solubility of RES was improved by approximately 1000-fold with PPI nanofibrils complex. DPPH and ABTS radical scavenging activity assays showed that the antioxidant capacity of RES was pronouncedly enhanced through the nanocomplexation with PPI nanofibrils. RES-PPI nanofibrils complexes exhibited higher antiproliferative activities than RES (free), with the cell viabilities of 52.6% and 38.5% for RES (free) and RES-PPI nanofibrils complex at 20 μg/mL. This study demonstrates that PPI nanofibrils can be utilized as novel nanocarriers for improvements of the water solubility, chemical stability and in vitro biological activities of hydrophobic nutraceuticals.
    MeSH term(s) Antioxidants ; Pea Proteins ; Resveratrol ; Solubility ; Water
    Chemical Substances Antioxidants ; Pea Proteins ; Water (059QF0KO0R) ; Resveratrol (Q369O8926L)
    Language English
    Publishing date 2021-12-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2021.131942
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Gasless submental-transoral combined approach endoscopic thyroidectomy: a new surgical technique.

    Jiang, Jinxi / He, Gaofei / Chu, Junjie / Li, Jianbo / Lu, Xiaoxiao / Jiang, Xianfeng / Xie, Lei / Gao, Li / Zhang, Deguang

    Frontiers in oncology

    2023  Volume 13, Page(s) 1115927

    Abstract: Background: The development of transoral endoscopic vestibular approach thyroidectomy (TOETVA) has been limited by inherent defects, such as mental nerve injury and carbon dioxide (CO: Methods: We reviewed 75 patients who successfully underwent ... ...

    Abstract Background: The development of transoral endoscopic vestibular approach thyroidectomy (TOETVA) has been limited by inherent defects, such as mental nerve injury and carbon dioxide (CO
    Methods: We reviewed 75 patients who successfully underwent gasless STET using novel instruments at our institution from November 2020 to November 2021. A main incision of approximately 2 cm was made in the natural submental crease line and then combined with two vestibule incisions to complete the procedure. Demographic data, surgical technique and perioperative outcomes were retrospectively recorded.
    Results: Thirteen male and sixty-two female patients with a mean age of 34.0 ± 8.1 years were enrolled in this study. Sixty-eight patients had papillary thyroid carcinomas and seven had benign nodules. We successfully performed all gasless STET without conversion to open surgery. The average postoperative hospital stay was 4.2 ± 1.8 days. One transient recurrent laryngeal nerve injury and two transient hypoparathyroidisms were observed. Three patients complained of slight lower lip numbness on the first postoperative day. One case of lymphatic fistula, subcutaneous effusion, and incision swelling occurred each, all of which were conservatively cured. One patient developed a recurrence six months after surgery.
    Conclusions: Gasless STET using our own designed suspension system is technically safe and feasible with reasonable operative and oncologic results.
    Language English
    Publishing date 2023-05-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1115927
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Positive regulation of the PhcB neighbouring regulator PrhX on expression of the type III secretion system and pathogenesis in Ralstonia solanacearum.

    Huang, Jiajun / Wang, Rongsheng / Zhang, Qi / Wang, Chunli / Liang, Tao / Hikichi, Yasufumi / Ohnishi, Kouhei / Jiang, Gaofei / Guo, Tao / Zhang, Yong

    Molecular plant pathology

    2023  Volume 25, Issue 1, Page(s) e13398

    Abstract: Ralstonia solanacearum PhcB and PhcA control a quorum-sensing (QS) system that globally regulates expression of about one third of all genes, including pathogenesis genes. The PhcB-PhcA QS system positively regulates the production of exopolysaccharide ( ... ...

    Abstract Ralstonia solanacearum PhcB and PhcA control a quorum-sensing (QS) system that globally regulates expression of about one third of all genes, including pathogenesis genes. The PhcB-PhcA QS system positively regulates the production of exopolysaccharide (EPS) and negatively regulates hrp gene expression, which is crucial for the type III secretion system (T3SS). Both EPS and the T3SS are essential for pathogenicity. The gene rsc2734 is located upstream of a phcBSR operon and annotated as a response regulator of a two-component system. Here, we demonstrated that RSc2734, hereafter named PrhX, positively regulated hrp gene expression via a PrhA-PrhIR-PrhJ-HrpG signalling cascade. Moreover, PrhX was crucial for R. solanacearum to invade host roots and grow in planta naturally. prhX expression was independent of the PhcB-PhcA QS system. PrhX did not affect the expression of phcB and phcA and the QS-dependent phenotypes, such as EPS production and biofilm formation. Our results provide novel insights into the complex regulatory network of the T3SS and pathogenesis in R. solanacearum.
    MeSH term(s) Ralstonia solanacearum ; Type III Secretion Systems/genetics ; Type III Secretion Systems/metabolism ; Cyanoacrylates/metabolism ; Virulence/genetics ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial
    Chemical Substances poly(hexyl-2-cyanoacrylate) (26877-39-4) ; Type III Secretion Systems ; Cyanoacrylates ; Bacterial Proteins
    Language English
    Publishing date 2023-10-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020755-4
    ISSN 1364-3703 ; 1364-3703
    ISSN (online) 1364-3703
    ISSN 1364-3703
    DOI 10.1111/mpp.13398
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  10. Article ; Online: Novel suspension system for gasless transoral vestibular thyroidectomy.

    Jiang, Jinxi / He, Gaofei / Chu, Junjie / Li, Jianbo / Lu, Xiaoxiao / Zhang, Deguang

    Surgical endoscopy

    2022  Volume 37, Issue 2, Page(s) 1070–1076

    Abstract: Background: The transoral endoscopic thyroidectomy vestibular approach (TOETVA) is receiving increased attention, frequently due to growing requirements for cosmetic incisions. Here, we report our initial experience and discuss the safety and efficacy ... ...

    Abstract Background: The transoral endoscopic thyroidectomy vestibular approach (TOETVA) is receiving increased attention, frequently due to growing requirements for cosmetic incisions. Here, we report our initial experience and discuss the safety and efficacy of the innovative surgical working space suspension system for gasless TOETVA.
    Methods: We retrospectively analyzed 75 consecutive patients for whom gasless TOETVA with our novel working space suspension system was used. This suspension system included self-developed retractors, a sterile bandage, and an anesthesia stand. We also improved some main surgical instruments in gasless TOETVA.
    Results: The study included 75 patients who successfully underwent thyroidectomy and central neck dissection via gasless TOETVA. The mean operating time was 143.27 ± 34.60 min. The mean number of retrieved lymph nodes was 8.00 ± 5.39. Conversion to open surgery did not occur, nor did patients exhibit serious postoperative complications. Postoperative complications included 4 cases of transient recurrent laryngeal nerve (RLN) palsy, 9 of transient hypoparathyroidism, and 3 of transient mental nerve injury. One patient with subcutaneous fluid after surgery recovered after aspiration. Another patient with submental minor perforation recovered well after suturing. There was no evidence of specific complications related to self-designed retractors.
    Conclusion: The innovative working space suspension system for gasless TOETVA provided enough and stable working space and optimized the clarity of the surgical field without CO
    MeSH term(s) Humans ; Thyroidectomy/adverse effects ; Retrospective Studies ; Natural Orifice Endoscopic Surgery/adverse effects ; Hypoparathyroidism/complications ; Hypoparathyroidism/surgery ; Vocal Cord Paralysis/etiology ; Postoperative Complications/etiology ; Thyroid Neoplasms/surgery
    Language English
    Publishing date 2022-09-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-022-09528-9
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