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  1. Article: Editorial: Utilization of microbiome to develop disease resistance in crop plants against phytopathogens.

    Ghosh, Srayan / Jha, Gopaljee

    Frontiers in plant science

    2023  Volume 14, Page(s) 1204896

    Language English
    Publishing date 2023-05-26
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1204896
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Methionine biosynthetic genes and methionine sulfoxide reductase A are required for Rhizoctonia solani AG1-IA to cause sheath blight disease in rice.

    Das, Joyati / Ghosh, Srayan / Tyagi, Kriti / Sahoo, Debashis / Jha, Gopaljee

    Microbial biotechnology

    2024  Volume 17, Issue 4, Page(s) e14441

    Abstract: Rhizoctonia solani is a polyphagous necrotrophic fungal pathogen that causes sheath blight disease in rice. It deploys effector molecules as well as carbohydrate-active enzymes and enhances the production of reactive oxygen species for killing host ... ...

    Abstract Rhizoctonia solani is a polyphagous necrotrophic fungal pathogen that causes sheath blight disease in rice. It deploys effector molecules as well as carbohydrate-active enzymes and enhances the production of reactive oxygen species for killing host tissues. Understanding R. solani ability to sustain growth under an oxidative-stress-enriched environment is important for developing disease control strategies. Here, we demonstrate that R. solani upregulates methionine biosynthetic genes, including Rs_MET13 during infection in rice, and double-stranded RNA-mediated silencing of these genes impairs the pathogen's ability to cause disease. Exogenous treatment with methionine restores the disease-causing ability of Rs_MET13-silenced R. solani and facilitates its growth on 10 mM H
    MeSH term(s) Methionine Sulfoxide Reductases ; Oryza/microbiology ; Methionine ; Hydrogen Peroxide/pharmacology ; Racemethionine/pharmacology ; Plant Diseases/microbiology ; Rhizoctonia
    Chemical Substances Methionine Sulfoxide Reductases (EC 1.8.4.-) ; Methionine (AE28F7PNPL) ; Hydrogen Peroxide (BBX060AN9V) ; Racemethionine (73JWT2K6T3)
    Language English
    Publishing date 2024-04-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.14441
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Host Gamma-Aminobutyric Acid Metabolic Pathway Is Involved in Resistance Against

    Rani, Mamta / Jha, Gopaljee

    Phytopathology

    2021  Volume 111, Issue 7, Page(s) 1207–1218

    Abstract: Rhizoctonia ... ...

    Abstract Rhizoctonia solani
    MeSH term(s) Metabolic Networks and Pathways ; Oryza ; Plant Diseases ; Rhizoctonia ; gamma-Aminobutyric Acid
    Chemical Substances gamma-Aminobutyric Acid (56-12-2)
    Language English
    Publishing date 2021-09-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208889-7
    ISSN 1943-7684 ; 0031-949X
    ISSN (online) 1943-7684
    ISSN 0031-949X
    DOI 10.1094/PHYTO-08-20-0356-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Nicotinic Acid Catabolism Modulates Bacterial Mycophagy in Burkholderia gladioli Strain NGJ1.

    Das, Joyati / Kumar, Rahul / Yadav, Sunil Kumar / Jha, Gopaljee

    Microbiology spectrum

    2023  Volume 11, Issue 3, Page(s) e0445722

    Abstract: Burkholderia gladioli strain NGJ1 exhibits mycophagous activity on a broad range of fungi, including Rhizoctonia solani, a devastating plant pathogen. Here, we demonstrate that the nicotinic acid (NA) catabolic pathway in NGJ1 is required for mycophagy. ... ...

    Abstract Burkholderia gladioli strain NGJ1 exhibits mycophagous activity on a broad range of fungi, including Rhizoctonia solani, a devastating plant pathogen. Here, we demonstrate that the nicotinic acid (NA) catabolic pathway in NGJ1 is required for mycophagy. NGJ1 is auxotrophic to NA and it potentially senses R. solani as a NA source. Mutation in the
    MeSH term(s) Burkholderia gladioli/metabolism ; Niacin/metabolism ; Bacteria/metabolism ; Biofilms ; Mutation ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial
    Chemical Substances Niacin (2679MF687A) ; Bacterial Proteins
    Language English
    Publishing date 2023-04-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.04457-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Nicotinic Acid Catabolism Modulates Bacterial Mycophagy in Burkholderia gladioli Strain NGJ1

    Joyati Das / Rahul Kumar / Sunil Kumar Yadav / Gopaljee Jha

    Microbiology Spectrum, Vol 11, Iss

    2023  Volume 3

    Abstract: ABSTRACT Burkholderia gladioli strain NGJ1 exhibits mycophagous activity on a broad range of fungi, including Rhizoctonia solani, a devastating plant pathogen. Here, we demonstrate that the nicotinic acid (NA) catabolic pathway in NGJ1 is required for ... ...

    Abstract ABSTRACT Burkholderia gladioli strain NGJ1 exhibits mycophagous activity on a broad range of fungi, including Rhizoctonia solani, a devastating plant pathogen. Here, we demonstrate that the nicotinic acid (NA) catabolic pathway in NGJ1 is required for mycophagy. NGJ1 is auxotrophic to NA and it potentially senses R. solani as a NA source. Mutation in the nicC and nicX genes involved in NA catabolism renders defects in mycophagy and the mutant bacteria are unable to utilize R. solani extract as the sole nutrient source. As supplementation of NA, but not FA (fumaric acid, the end product of NA catabolism) restores the mycophagous ability of ΔnicC/ΔnicX mutants, we anticipate that NA is not required as a carbon source for the bacterium during mycophagy. Notably, nicR, a MarR-type of transcriptional regulator that functions as a negative regulator of the NA catabolic pathway is upregulated in ΔnicC/ΔnicX mutant and upon NA supplementation the nicR expression is reduced to the basal level in both the mutants. The ΔnicR mutant produces excessive biofilm and is completely defective in swimming motility. On the other hand, ΔnicC/ΔnicX mutants are compromised in swimming motility as well as biofilm formation, potentially due to the upregulation of nicR. Our data suggest that a defect in NA catabolism alters the NA pool in the bacterium and upregulates nicR which in turn suppresses bacterial motility as well as biofilm formation, leading to mycophagy defects. IMPORTANCE Mycophagy is an important trait through which certain bacteria forage over fungal mycelia and utilize fungal biomass as a nutrient source to thrive in hostile environments. The present study emphasizes that nicotinic acid (NA) is important for bacterial motility and biofilm formation during mycophagy by Burkholderia gladioli strain NGJ1. Defects in NA catabolism potentially alter the cellular NA pool, upregulate the expression of nicR, a negative regulator of biofilm, and therefore suppress bacterial motility as well as biofilm formation, leading to ...
    Keywords bacterial-fungal interaction ; bacteriology ; Burkholderia gladioli ; environmental microbiology ; genetics and molecular biology ; mycophagy ; Microbiology ; QR1-502
    Subject code 572
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher American Society for Microbiology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Host Gamma-Aminobutyric Acid Metabolic Pathway Is Involved in Resistance Against Rhizoctonia solani

    Rani, Mamta / Jha, Gopaljee

    Phytopathology. 2021 July, v. 111, no. 7

    2021  

    Abstract: Rhizoctonia solani is a highly destructive necrotrophic fungal pathogen having a diverse host range, including rice and tomato. Previously R. solani infection has been found to cause large-scale readjustment in host primary metabolism and accumulation of ...

    Abstract Rhizoctonia solani is a highly destructive necrotrophic fungal pathogen having a diverse host range, including rice and tomato. Previously R. solani infection has been found to cause large-scale readjustment in host primary metabolism and accumulation of various stress-associated metabolites such as gamma-aminobutyric acid (GABA) in rice. In this study, we report upregulation of GABA pathway genes during pathogenesis of R. solani in rice and tomato. The exogenous application of GABA provided partial resistance against R. solani infection in both the hosts. Furthermore, by using the virus-induced gene silencing approach, we knocked down the expression of some of the tomato genes involved in GABA biosynthesis (glutamate decarboxylase) and GABA catabolism (GABA-transaminase and succinic semialdehyde dehydrogenase) to study their role in host defense against R. solani infection. The silencing of each of these genes increased disease susceptibility in tomato. Overall the results from gene expression analysis, exogenous chemical application, and gene silencing studies suggest that the GABA pathway plays a positive role in plant defense against necrotrophic pathogen R. solani.
    Keywords Thanatephorus cucumeris ; biochemical pathways ; biosynthesis ; catabolism ; disease susceptibility ; fungi ; gamma-aminobutyric acid ; gene expression ; genes ; glutamate decarboxylase ; host range ; metabolites ; oxidoreductases ; pathogenesis ; pathogens ; plant pathology ; rice ; tomatoes
    Language English
    Dates of publication 2021-07
    Size p. 1207-1218.
    Publishing place The American Phytopathological Society
    Document type Article
    ZDB-ID 208889-7
    ISSN 1943-7684 ; 0031-949X
    ISSN (online) 1943-7684
    ISSN 0031-949X
    DOI 10.1094/PHYTO-08-20-0356-R
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Draft Genome Sequence of

    Mishra, Arti / Jha, Gopaljee / Thakur, Indu Shekhar

    Genome announcements

    2018  Volume 6, Issue 5

    Abstract: ... ...

    Abstract Zhihengliuella
    Language English
    Publishing date 2018-02-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.01533-17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: RS_CRZ1

    Ghosh, Srayan / Kant, Ravi / Pradhan, Amrita / Jha, Gopaljee

    Molecular plant-microbe interactions : MPMI

    2020  Volume 34, Issue 1, Page(s) 26–38

    Abstract: Rhizoctonia ... ...

    Abstract Rhizoctonia solani
    MeSH term(s) Gene Silencing ; Lycopersicon esculentum/microbiology ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Rhizoctonia/genetics ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Up-Regulation
    Chemical Substances Transcription Factors
    Language English
    Publishing date 2020-12-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-05-20-0121-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: N-Terminus Plays a Critical Role for Stabilizing the Filamentous Assembly and the Antifungal Activity of Bg_9562.

    Sahoo, Pankaj Kumar / Kumar, Rahul / Rani, Aruna / Yadav, Sunil Kumar / Jha, Gopaljee / Jain, Deepti

    Microbiology spectrum

    2022  Volume 10, Issue 5, Page(s) e0160722

    Abstract: Bg_9562, a prophage tail-like protein was earlier shown to be required for bacterial mycophagy by Burkholderia gladioli strain NGJ1. The purified protein exhibited broad-spectrum antifungal activity; however, the structural and mechanistic details vis-à- ... ...

    Abstract Bg_9562, a prophage tail-like protein was earlier shown to be required for bacterial mycophagy by Burkholderia gladioli strain NGJ1. The purified protein exhibited broad-spectrum antifungal activity; however, the structural and mechanistic details vis-à-vis its activity remained elusive. In this study, we have structurally characterized the protein Bg_9562 using negatively stained transmission electron microscopy, molecular modeling and mutagenesis. We find that Bg_9562 shows structural similarity to Gp13, a tail assembly chaperone. The transmission electron microscopy revealed that, Bg_9562 forms long flexible tubular structures. Molecular modeling of the filament like structure divulges that the inter subunit contacts are meditated largely through hydrophobic interactions. Using mutagenesis, we demonstrate that the N-terminal residues of the protein when deleted results in reduced activity and destabilization of filament formation. Overall, structure-function analysis opens up avenues for further utilization of the protein as a potent antifungal molecule.
    MeSH term(s) Animals ; Humans ; Antifungal Agents/pharmacology ; Plant Diseases/microbiology
    Chemical Substances Antifungal Agents
    Language English
    Publishing date 2022-08-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01607-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Alterations in plant sugar metabolism: signatory of pathogen attack.

    Kanwar, Poonam / Jha, Gopaljee

    Planta

    2018  Volume 249, Issue 2, Page(s) 305–318

    Abstract: Main conclusion: This review summarizes the current understanding, future challenges and ongoing quest on sugar metabolic alterations that influence the outcome of plant-pathogen interactions. Intricate cellular and molecular events occur during plant- ... ...

    Abstract Main conclusion: This review summarizes the current understanding, future challenges and ongoing quest on sugar metabolic alterations that influence the outcome of plant-pathogen interactions. Intricate cellular and molecular events occur during plant-pathogen interactions. They cause major metabolic perturbations in the host and alterations in sugar metabolism play a pivotal role in governing the outcome of various kinds of plant-pathogen interactions. Sugar metabolizing enzymes and transporters of both host and pathogen origin get differentially regulated during the interactions. Both plant and pathogen compete for utilizing the host sugar metabolic machinery and in turn promote resistant or susceptible responses. However, the kind of sugar metabolism alteration that is beneficial for the host or pathogen is yet to be properly understood. Recently developed tools and methodologies are facilitating research to understand the intricate dynamics of sugar metabolism during the interactions. The present review elaborates current understanding, future challenges and ongoing quest on sugar metabolism, mobilization and regulation during various plant-pathogen interactions.
    MeSH term(s) Plant Diseases ; Plant Immunity ; Plants/metabolism ; Sugars/metabolism
    Chemical Substances Sugars
    Language English
    Publishing date 2018-09-28
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-018-3018-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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