Article ; Online: Flowering Poration-A Synergistic Multi-Mode Antibacterial Mechanism by a Bacteriocin Fold.
iScience
2020 Volume 23, Issue 8, Page(s) 101423
Abstract: Bacteriocins are a distinct family of antimicrobial proteins postulated to porate bacterial membranes. However, direct experimental evidence of pore formation by these proteins is lacking. Here we report a multi-mode poration mechanism induced by four- ... ...
| Abstract | Bacteriocins are a distinct family of antimicrobial proteins postulated to porate bacterial membranes. However, direct experimental evidence of pore formation by these proteins is lacking. Here we report a multi-mode poration mechanism induced by four-helix bacteriocins, epidermicin NI01 and aureocin A53. Using a combination of crystallography, spectroscopy, bioassays, and nanoscale imaging, we established that individual two-helix segments of epidermicin retain antibacterial activity but each of these segments adopts a particular poration mode. In the intact protein these segments act synergistically to balance out antibacterial and hemolytic activities. The study sets a precedent of multi-mode membrane disruption advancing the current understanding of structure-activity relationships in pore-forming proteins. |
|---|---|
| Language | English |
| Publishing date | 2020-07-30 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 2589-0042 |
| ISSN (online) | 2589-0042 |
| DOI | 10.1016/j.isci.2020.101423 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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