Article ; Online: Tenglong Buzhong granules inhibits the growth of SW620 human colon cancer.
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
2022 Volume 42, Issue 5, Page(s) 701–706
Abstract: Objective: To observe the anticancer effects of the granular preparation of Tenglong Buzhong ... decoction (,TBD), i.e Tenglong Buzhong granules (, TBG), in human SW620 colon cancer.: Methods: BALB/c ...
| Abstract | Objective: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction (,TBD), i.e Tenglong Buzhong granules (, TBG), in human SW620 colon cancer. Methods: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG (2.56 g/kg, once per day) and/or 5-Fu (104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry. Results: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anti-cancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3, -8, and -9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a (CDKN1a) and cyclin-dependent kinase inhibitor 2a (CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein (RB) and expression of cyclin E1 (CCNE1) and cyclin-dependent kinases (CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α). Conclusions: TBG inhibits SW620 colon cancer growth, induces apoptosis SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu. |
|---|---|
| MeSH term(s) | Animals ; Cell Line, Tumor ; Colonic Neoplasms/drug therapy ; Colonic Neoplasms/genetics ; Colonic Neoplasms/metabolism ; Cyclin-Dependent Kinases ; Cyclins ; Fluorouracil ; Humans ; Mice ; Mice, Nude ; Neovascularization, Pathologic/pathology ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism |
| Chemical Substances | Cyclins ; Vascular Endothelial Growth Factor A ; Cyclin-Dependent Kinases (EC 2.7.11.22) ; Fluorouracil (U3P01618RT) |
| Language | English |
| Publishing date | 2022-10-26 |
| Publishing country | China |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 603186-9 |
| ISSN | 2589-451X ; 0254-6272 ; 0255-2922 |
| ISSN (online) | 2589-451X ; 0254-6272 |
| ISSN | 0255-2922 |
| DOI | 10.19852/j.cnki.jtcm.2022.05.004 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.B 2564: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.