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Article ; Online: Evaluation of bactericidal efficacy of silver ions on Escherichia coli for drinking water disinfection.

Pathak, Satya P / Gopal, K

Environmental science and pollution research international

2012 Volume 19, Issue 6, Page(s) 2285–2290

Abstract: Purpose: The purpose of this study is the development of a suitable process for the disinfection of drinking water by evaluating bactericidal efficacy of silver ions from silver electrodes.: Methods: A prototype of a silver ioniser with silver ... ...

Abstract	Purpose: The purpose of this study is the development of a suitable process for the disinfection of drinking water by evaluating bactericidal efficacy of silver ions from silver electrodes. Methods: A prototype of a silver ioniser with silver electrodes and control unit has been fabricated. Silver ions from silver electrodes in water samples were estimated with an atomic absorption spectrophotometer. A fresh culture of Escherichia coli (1.75 × 10(3) c.f.u./ml) was exposed to 1, 2, 5, 10 and 20 ppb of silver ions in 100 ml of autoclaved tap water for 60 min. The effect of different pH and temperatures on bactericidal efficacy was observed at constant silver ion concentration (5 ppb) and contact time of 30 min. Results: The maximum bactericidal activity (100%) was observed at 20 ppb of silver ion concentration indicating total disinfection after 20 min while minimum bactericidal activity (25%) was observed after 10 min at 01 ppb of silver ions. Likewise, 100% bactericidal activity was noticed with 2, 5 and 10 ppb of silver ions after 60, 50 and 40 min, respectively. Bactericidal activity at pH 5, 6, 7, 8 and 9 was observed at 79.9%, 79.8%, 80.5%, 100% and 100%, respectively, whereas it was 80.4%, 88.3%, 100%, 100% and 100% at 10°C, 20°C, 30°C, 40°C and 50°C, respectively. Conclusion: The findings of this study revealed that very low concentrations of silver ions at pH 8-9 and temperature >20°C have bactericidal efficacy for total disinfection of drinking water. Silver ionisation is suitable for water disinfection and an appropriate alternative to chlorination which forms carcinogenic disinfection by-products.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Disinfection/methods ; Dose-Response Relationship, Drug ; Drinking Water/analysis ; Drinking Water/microbiology ; Escherichia coli/drug effects ; Hydrogen-Ion Concentration ; Silver/analysis ; Silver/pharmacology ; Spectrophotometry, Atomic ; Temperature ; Water Purification/methods
Chemical Substances	Anti-Bacterial Agents ; Drinking Water ; Silver (3M4G523W1G)
Language	English
Publishing date	2012-01-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-011-0735-6
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Article: NAD+ depletion enhances reovirus-induced oncolysis in multiple myeloma.

Kennedy, Barry E / Giacomantonio, Michael / Murphy, J Patrick / Cutler, Samuel / Sadek, Maryanne / Konda, Prathyusha / Paulo, Joao A / Pathak, Gopal P / Renkens, Saskia H J / Grieve, Stacy / Pol, Jonathan / Gygi, Steven P / Richardson, Christopher / Gaston, Daniel / Reiman, Anthony / Kroemer, Guido / Elnenaei, Manal O / Gujar, Shashi A

Molecular therapy oncolytics

2022 Volume 24, Page(s) 695–706

Abstract: Cancer cell energy metabolism plays an important role in dictating the efficacy of oncolysis by oncolytic viruses. To understand the role of multiple myeloma metabolism in reovirus oncolysis, we performed semi-targeted mass spectrometry-based ... ...

Abstract	Cancer cell energy metabolism plays an important role in dictating the efficacy of oncolysis by oncolytic viruses. To understand the role of multiple myeloma metabolism in reovirus oncolysis, we performed semi-targeted mass spectrometry-based metabolomics on 12 multiple myeloma cell lines and revealed a negative correlation between NAD+ levels and susceptibility to oncolysis. Likewise, a negative correlation was observed between the activity of the rate-limiting NAD+ synthesis enzyme NAMPT and oncolysis. Indeed, depletion of NAD+ levels by pharmacological inhibition of NAMPT using FK866 sensitized several myeloma cell lines to reovirus-induced killing. The myelomas that were most sensitive to this combination therapy expressed a functional p53 and had a metabolic and transcriptomic profile favoring mitochondrial metabolism over glycolysis, with the highest synergistic effect in KMS12 cells. Mechanistically, U-
Language	English
Publishing date	2022-02-20
Publishing country	United States
Document type	Journal Article
ISSN	2372-7705
ISSN	2372-7705
DOI	10.1016/j.omto.2022.02.017
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Article: Bacterial contamination and antibiotic resistance in fecal coliforms from glacial water runoff.

Pathak, S P / Gopal, K

Bulletin of environmental contamination and toxicology

2007 Volume 79, Issue 2, Page(s) 163–167

MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial ; Environmental Monitoring ; Escherichia coli/drug effects ; Escherichia coli/isolation & purification ; Feces/microbiology ; Fresh Water/microbiology ; Ice Cover ; India ; Water Microbiology ; Water Pollution/analysis ; Water Supply/analysis
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2007-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	6895-0
ISSN	1432-0800 ; 0007-4861
ISSN (online)	1432-0800
ISSN	0007-4861
DOI	10.1007/s00128-007-9128-4
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Article: Prevalence of bacterial contamination with antibiotic-resistant and enterotoxigenic fecal coliforms in treated drinking water.

Pathak, S P / Gopal, K

Journal of toxicology and environmental health. Part A

2008 Volume 71, Issue 7, Page(s) 427–433

Abstract: Pollution indicator bacteria such as coliforms, fecal coliforms, and fecal streptococci were enumerated using a multiple-tube fermentation method in 100 treated drinking-water samples from 20 locations in residential, commercial, and industrial areas of ... ...

Abstract	Pollution indicator bacteria such as coliforms, fecal coliforms, and fecal streptococci were enumerated using a multiple-tube fermentation method in 100 treated drinking-water samples from 20 locations in residential, commercial, and industrial areas of a tropical city during summer. Thirty-four percent of the samples were bacteriologically nonpotable. Maximum coliform-contaminated (27%) samples were derived from industrial areas, while samples contaminated with fecal coliform (23%) and fecal streptococci (20%) originated from commercial areas. Coliforms identified as Escherichia coli, Klebsiella sp., Enterobacter sp., and Citrobacter sp. were present in 29%, 26%, 24%, and 15% of samples, respectively. Fecal coliforms were examined for antibiotic susceptibility with disc diffusion method. All test isolates exhibited multiple antibiotic resistance (MAR) for kanamycin, nalidixic acid, tetracycline, and trimethoprim. Escherichia coli isolates were examined for enterotoxigenicity using the suckling mice bioassay and 60% of the isolates displayed enterotoxigenicity. Data indicate that drinking water contaminated with antibiotic-resistant enterotoxigenic fecal bacteria may be responsible for presence of waterborne diarrheal diseases attributed to therapeutic agents used by urban populations in the tropics.
MeSH term(s)	Colony Count, Microbial ; Decontamination/methods ; Drug Resistance, Multiple, Bacterial ; Enterotoxigenic Escherichia coli/drug effects ; Enterotoxigenic Escherichia coli/isolation & purification ; Environmental Monitoring ; Fresh Water/microbiology ; Humans ; India ; Water Microbiology ; Water Pollution/analysis ; Water Pollution/prevention & control ; Water Supply/analysis
Language	English
Publishing date	2008
Publishing country	England
Document type	Journal Article
ZDB-ID	1413345-3
ISSN	1528-7394 ; 0098-4108 ; 1087-2620
ISSN	1528-7394 ; 0098-4108 ; 1087-2620
DOI	10.1080/15287390701838796
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Article ; Online: Combined effect of substance P and curcumin on cutaneous wound healing in diabetic rats.

Kant, Vinay / Kumar, Dinesh / Prasad, Raju / Gopal, Anu / Pathak, Nitya N / Kumar, Pawan / Tandan, Surender K

The Journal of surgical research

2017 Volume 212, Page(s) 130–145

Abstract: Background: Our earlier studies demonstrated that topically applied substance P (SP) or curcumin ...

Abstract	Background: Our earlier studies demonstrated that topically applied substance P (SP) or curcumin on excision skin wound accelerated the wound healing in streptozotocin-induced diabetic rats. In the present study, we aimed to evaluate the wound healing potential of combination of SP and curcumin in diabetic rats. Materials and methods: Open cutaneous excision wound was created on the back of each of the 60 diabetic rats. Wound-inflicted rats were equally divided into three groups namely, control, gel treated, and SP + curcumin treated. Normal saline, pluronic gel, and SP (0.5 × 10 Results: SP + curcumin combination significantly accelerated wound closure and decreased messenger RNA expressions of tumor necrosis factor-alpha, interleukin-1beta, and matrix metalloproteinase-9, whereas the combination markedly increased the expressions of interleukin-10, vascular endothelial growth factor, transforming growth factor-beta1, hypoxia-inducible factor 1-alpha, stromal cell-derived factors-1alpha, heme oxygenase-1 and endothelial nitric oxide synthase, and activities of superoxide dismutase, catalase, and glutathione peroxidase in granulation-healing tissue, compared with control and gel-treated groups. In combination group, granulation tissue was better, as was evidenced by improved fibroblast proliferation, collagen deposition, microvessel density, growth-associated protein 43-positive nerve fibers, and thick regenerated epithelial layer. Conclusions: The combination of SP and curcumin accelerated wound healing in diabetic rats and both the drugs were compatible at the doses used in this study.
MeSH term(s)	Administration, Cutaneous ; Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Biomarkers/metabolism ; Curcumin/pharmacology ; Curcumin/therapeutic use ; Diabetes Mellitus, Experimental/complications ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Experimental/pathology ; Drug Therapy, Combination ; Male ; Neurotransmitter Agents/pharmacology ; Neurotransmitter Agents/therapeutic use ; Rats ; Rats, Wistar ; Skin/drug effects ; Skin/injuries ; Skin/metabolism ; Skin/pathology ; Substance P/pharmacology ; Substance P/therapeutic use ; Treatment Outcome ; Wound Healing/drug effects ; Wound Healing/physiology ; Wounds and Injuries/complications ; Wounds and Injuries/drug therapy ; Wounds and Injuries/metabolism ; Wounds and Injuries/pathology
Chemical Substances	Anti-Inflammatory Agents ; Biomarkers ; Neurotransmitter Agents ; Substance P (33507-63-0) ; Curcumin (IT942ZTH98)
Language	English
Publishing date	2017-01-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2017.01.011
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Article ; Online: Strong Anharmonicity-Induced Low Thermal Conductivity and High n-type Mobility in the Topological Insulator Bi

Pathak, Riddhimoy / Dutta, Prabir / Srivastava, Ashutosh / Rawat, Divya / Gopal, Radha Krishna / Singh, Abhishek K / Soni, Ajay / Biswas, Kanishka

Angewandte Chemie (International ed. in English)

2022 Volume 61, Issue 41, Page(s) e202210783

Abstract: Intrinsically low lattice thermal conductivity ( ... ...

Abstract	Intrinsically low lattice thermal conductivity (κ
Language	English
Publishing date	2022-09-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202210783
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Article ; Online: Detection and isolation of selected genes of interest from metagenomic libraries by a DNA microarray approach.

Pathak, Gopal P / Gärtner, Wolfgang

Methods in molecular biology (Clifton, N.J.)

2010 Volume 668, Page(s) 299–312

Abstract: A DNA microarray-based approach is described for screening metagenomic libraries for the presence of selected genes. The protocol is exemplified for the identification of flavin-binding, blue-light-sensitive biological photoreceptors (BL), based on a ... ...

Abstract	A DNA microarray-based approach is described for screening metagenomic libraries for the presence of selected genes. The protocol is exemplified for the identification of flavin-binding, blue-light-sensitive biological photoreceptors (BL), based on a homology search in already sequenced, annotated genomes. The microarray carried 149 different 54-mer oligonucleotides, derived from consensus sequences of BL photoreceptors. The array could readily identify targets carrying 4% sequence mismatch, and allowed unambiguous identification of a positive cosmid clone of as little as 10 ng against a background of 25 μg of cosmid DNA. The protocol allows screening up to 1,200 library clones in concentrations as low as ca. 20 ng, each with a ca. 40 kb insert size readily in a single batch. Calibration and control conditions are outlined. This protocol, when applied to the thermophilic fraction of a soil sample, yielded the identification and functional characterization of a novel, BL-encoding gene that showed a 58% similarity to a known, BL-encoding gene from Kineococcus radiotolerans SRS30216 (similarity values refer to the respective LOV domains).
MeSH term(s)	Amino Acid Sequence ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Gene Library ; Genes, Bacterial ; Metagenome ; Metagenomics/instrumentation ; Metagenomics/methods ; Molecular Sequence Data ; Molecular Structure ; Oligonucleotide Array Sequence Analysis/instrumentation ; Oligonucleotide Array Sequence Analysis/methods ; Sequence Alignment
Chemical Substances	DNA, Bacterial
Language	English
Publishing date	2010
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-60761-823-2_21
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Article ; Online: Identifying social determinants of health from clinical narratives: A study of performance, documentation ratio, and potential bias.

Yu, Zehao / Peng, Cheng / Yang, Xi / Dang, Chong / Adekkanattu, Prakash / Gopal Patra, Braja / Peng, Yifan / Pathak, Jyotishman / Wilson, Debbie L / Chang, Ching-Yuan / Lo-Ciganic, Wei-Hsuan / George, Thomas J / Hogan, William R / Guo, Yi / Bian, Jiang / Wu, Yonghui

Journal of biomedical informatics

2024 Volume 153, Page(s) 104642

Abstract: Objective: To develop a natural language processing (NLP) package to extract social determinants of health (SDoH) from clinical narratives, examine the bias among race and gender groups, test the generalizability of extracting SDoH for different disease ...

Abstract	Objective: To develop a natural language processing (NLP) package to extract social determinants of health (SDoH) from clinical narratives, examine the bias among race and gender groups, test the generalizability of extracting SDoH for different disease groups, and examine population-level extraction ratio. Methods: We developed SDoH corpora using clinical notes identified at the University of Florida (UF) Health. We systematically compared 7 transformer-based large language models (LLMs) and developed an open-source package - SODA (i.e., SOcial DeterminAnts) to facilitate SDoH extraction from clinical narratives. We examined the performance and potential bias of SODA for different race and gender groups, tested the generalizability of SODA using two disease domains including cancer and opioid use, and explored strategies for improvement. We applied SODA to extract 19 categories of SDoH from the breast (n = 7,971), lung (n = 11,804), and colorectal cancer (n = 6,240) cohorts to assess patient-level extraction ratio and examine the differences among race and gender groups. Results: We developed an SDoH corpus using 629 clinical notes of cancer patients with annotations of 13,193 SDoH concepts/attributes from 19 categories of SDoH, and another cross-disease validation corpus using 200 notes from opioid use patients with 4,342 SDoH concepts/attributes. We compared 7 transformer models and the GatorTron model achieved the best mean average strict/lenient F1 scores of 0.9122 and 0.9367 for SDoH concept extraction and 0.9584 and 0.9593 for linking attributes to SDoH concepts. There is a small performance gap (∼4%) between Males and Females, but a large performance gap (>16 %) among race groups. The performance dropped when we applied the cancer SDoH model to the opioid cohort; fine-tuning using a smaller opioid SDoH corpus improved the performance. The extraction ratio varied in the three cancer cohorts, in which 10 SDoH could be extracted from over 70 % of cancer patients, but 9 SDoH could be extracted from less than 70 % of cancer patients. Individuals from the White and Black groups have a higher extraction ratio than other minority race groups. Conclusions: Our SODA package achieved good performance in extracting 19 categories of SDoH from clinical narratives. The SODA package with pre-trained transformer models is available at https://github.com/uf-hobi-informatics-lab/SODA_Docker.
MeSH term(s)	Humans ; Natural Language Processing ; Social Determinants of Health ; Female ; Male ; Narration ; Bias ; Electronic Health Records ; Documentation/methods ; Data Mining/methods
Language	English
Publishing date	2024-04-14
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2057141-0
ISSN	1532-0480 ; 1532-0464
ISSN (online)	1532-0480
ISSN	1532-0464
DOI	10.1016/j.jbi.2024.104642
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Article: Evaluation of the presence-absence (P-A) test: A simplified bacteriological test for detecting coliforms in rural drinking water of India.

Ramteke, P W / Pathak, S P / Bhattacherjee, J W / Gopal, K / Mathur, N

Environmental monitoring and assessment

2013 Volume 33, Issue 1, Page(s) 53–59

Abstract: ... surface water and piped supplies were tested in order to compare the presence-absence (P-A) test with standard ... 1030 (74.88%) were positive by the MPN and P-A test, respectively. The P-A test detected 96 ... of the positives detected by the MPN test. The P-A test may be effectively used as a rapid screening method ...

Abstract	One thousand three-hundred and ninety-four drinking water sources comprising ground water, surface water and piped supplies were tested in order to compare the presence-absence (P-A) test with standard MPN method to detect coliforms as indicators of water quality. Out of 1394 samples, 1074 (77.04%) and 1030 (74.88%) were positive by the MPN and P-A test, respectively. The P-A test detected 96% of the positives detected by the MPN test. The P-A test may be effectively used as a rapid screening method to detect coliform contamination in less polluted sources such as ground water and piped supplies.
Language	English
Publishing date	2013-11-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/BF00546661
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Article: Occurrence of antibiotic and metal resistance in bacteria from organs of river fish.

Pathak, S P / Gopal, K

Environmental research

2005 Volume 98, Issue 1, Page(s) 100–103

Abstract: Bacterial populations in some organs, viz., liver, spleen, kidney, gill, and arborescent organ of the catfish Clarias batrachus were enumerated followed by determination of resistance for antibiotics and metals. The total viable counts in these organs, ... ...

Abstract	Bacterial populations in some organs, viz., liver, spleen, kidney, gill, and arborescent organ of the catfish Clarias batrachus were enumerated followed by determination of resistance for antibiotics and metals. The total viable counts in these organs, observed, were 2.24x10(4), 2.08x10(4), 1.44x10(4), 1.23x10(4), and 6.40x10(3) colony-forming units/mL, respectively. The random bacterial isolates from these fish organs showed resistance in decreasing order for colistin (98%), ampicillin (82%), gentamycin (34%), carbenicillin (28%), tetracyline (20%), streptomycin (12%), and ciprofloxacin (02%). Most of the isolates exhibited an increasing order of tolerance for the metals (microg/mL) copper (100), lead (200), manganese (400), cadmium (200), and chromium (50), with minimum inhibitory concentration (MIC) ranging from <50 to 1600 microg/mL. These observations indicate that the significant occurrence of bacterial population in organs of fish with high incidence of resistance for antibiotics and metals may pose risk to fish fauna and public health.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Bacteria/drug effects ; Catfishes/microbiology ; Drug Resistance, Bacterial/physiology ; Metals, Heavy/pharmacology ; Microbial Sensitivity Tests ; Rivers ; Water Pollutants, Chemical/poisoning
Chemical Substances	Anti-Bacterial Agents ; Metals, Heavy ; Water Pollutants, Chemical
Language	English
Publishing date	2005-05
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2004.05.012
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