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Article ; Online: Coordination-triggered redox activity of early and late lanthanide calix[4]arene complexes.

Jiao, Yushu / Sanz, Sergio / Koláčná, Lucie / van Leusen, Jan / Izarova, Natalya V / Sarwar, Sidra / Ludvík, Jiří / Kögerler, Paul

Dalton transactions (Cambridge, England : 2003)

2024 Volume 53, Issue 11, Page(s) 4930–4936

Abstract: The methylation ... ...

Abstract	The methylation of
Language	English
Publishing date	2024-03-12
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d3dt03828a
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Article ; Online: Designing Powerful Biindole-Based Inherently Chiral Selectors: Enhancing Enantiodiscrimination by Core Functionalization with Additional Coordination Elements.

Grecchi, Sara / Bonetti, Giorgia / Emanuele, Elisa / Ludvík, Jiří / Koláčná, Lucie / Liška, Alan / Hromadová, Magdaléna / Arnaboldi, Serena / Cirilli, Roberto / Mussini, Patrizia R / Benincori, Tiziana

Chemistry (Weinheim an der Bergstrasse, Germany)

2024 Volume 30, Issue 23, Page(s) e202303530

Abstract: Among inherently chiral selectors of axial stereogenicity, usually resulting in very good enantiodiscrimination performances, the biindole-based family has the additional advantage of very easy functionalization of the two nitrogen atoms with a variety ... ...

Abstract	Among inherently chiral selectors of axial stereogenicity, usually resulting in very good enantiodiscrimination performances, the biindole-based family has the additional advantage of very easy functionalization of the two nitrogen atoms with a variety of substituents with desirable properties. Aiming to evaluate the possibility of exploiting such feature to enhance the enantiodiscrimination ability of the archetype structure, a series of three inherently chiral monomers were designed and synthesized, characterised by a 2,2'-biindole atropisomeric core conjugated to bithiophene wings enabling fast and regular electrooligomerization, and functionalised at the nitrogen atoms with an ethyl, a methoxyethyl, or a hydroxyethyl substituent. Nitrogen alkylation was also exploited to obtain for the first time the chemical resolution of the biindole selectors without employing chiral HPLC. The enantiodiscrimination ability of the selector series was comparatively evaluated in proof-of-concept chiral voltammetry experiments with a "benchmark" chiral ferrocenyl probe as well as with chiral non-steroidal anti-inflammatory drugs naproxen and ketoprofen. The large enantiomer potential differences for all probes increased in the ethyl < methoxyethyl ≪ hydroxyethyl sequence of selector substituents, supporting our assumption on the beneficial role of an additional coordination element. The powerful hydroxyethyl selector was also applied to ketoprofen in a commercial drug matrix.
Language	English
Publishing date	2024-03-22
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202303530
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Article: Photosynthesis by developing embryos of oilseed rape (Brassica napus L.)

Eastmond, P / Kolacna, L / Rawsthrone, S

Journal of experimental botany. Nov 1996. v. 47 (304)

1996

Abstract: ... rape (Brassica napus L.) and to compare photosynthetic properties of embryo plastics with those of leaf ...

Abstract	The aim of this study was to assess the photosynthetic potential of developing seeds of oilseed rape (Brassica napus L.) and to compare photosynthetic properties of embryo plastics with those of leaf chloroplasts from the same species. Measurements of CO2-dependent O2 evolution show that developing seeds of B. napus are photosynthetically active in vitro. Essentially, all of the photosynthetic activity of the developing seed is accounted for by the embryo. The rate of photosynthesis by developing embryos increased until the onset of desiccation, after which it declined, so that by maturity embryos were no longer photosynthetically active. Photosynthetic activity was positively correlated with chlorophyll content throughout development. Comparison of the photosynthetic characteristics of leaf and embryo chloroplasts revealed that rates of uncoupled electron transport were 2.5-fold greater in those from the embryo. Light-saturated rates of CO2-dependent O2 evolution, per unit chlorophyll, and CO2 saturation points were similar for chloroplasts from both tissues. However, light-saturation points and chlorophyll a/b ratios were lower for embryo than for leaf choroplasts. Embryos and embryo chloroplasts also contained considerably less ribulose 1,5-bisphosphate carboxylase/oxygenase protein per unit total protein, than leaves. Although excised embryos were capable of high rates of CO2-dependent O2 evolution (90-100 micromoles mg chlorophyll h-1) under a saturating photosynthetic photon flux density (PPFD), low transmittance of light through the silique wall (30%), together with the high PPFD required to achieve light compensation points in developing seeds (500 micromoles m-2 s-1), suggests that photosynthesis in vivo is unlikely to make a net contribution to carbon economy under normal environmental conditions.
Keywords	gas production (biological) ; Brassica napus ; photosynthesis ; embryo (plant) ; plastids ; seeds ; chloroplasts ; leaves ; chlorophyll ; oxygen ; ribulose-bisphosphate carboxylase ; light ; transmittance ; electron transfer
Language	English
Dates of publication	1996-11
Size	p. 1763-1769.
Document type	Article
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
Database	NAL-Catalogue (AGRICOLA)

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Article: The pi-helix formation between Asp369 and Thr375 as a key factor in E1-E2 conformational change of Na+/K+-ATPase.

Tejral, G / Koláčná, L / Schoner, W / Amler, E

Physiological research

2008 Volume 58, Issue 4, Page(s) 583–589

Abstract: Molecular modeling of the H4-H5-loop of the alpha2 isoform of Na+/K+-ATPase in the E1 and E2 conformations revealed that twisting of the nucleotide (N) domain toward the phosphorylation (P) domain is connected with the formation of a short pi-helix ... ...

Abstract	Molecular modeling of the H4-H5-loop of the alpha2 isoform of Na+/K+-ATPase in the E1 and E2 conformations revealed that twisting of the nucleotide (N) domain toward the phosphorylation (P) domain is connected with the formation of a short pi-helix between Asp369 and Thr375. This conformational change close to the hinge region between the N-domain and the P-domain could be an important event leading to a bending of the N-domain by 64.7 degrees and to a shortening of the distance between the ATP binding site and the phosphorylation site (Asp369) by 1.22 nm from 3.22 nm to 2.00 nm. It is hypothesized that this shortening mechanism is involved in the Na+-dependent formation of the Asp369 phospho-intermediate as part of the overall Na+/K+-ATPase activity.
MeSH term(s)	Aspartic Acid/chemistry ; Aspartic Acid/genetics ; Binding Sites ; Kinetics ; Models, Molecular ; Protein Isoforms/chemistry ; Protein Isoforms/metabolism ; Protein Structure, Secondary ; Sodium-Potassium-Exchanging ATPase/chemistry ; Sodium-Potassium-Exchanging ATPase/genetics ; Sodium-Potassium-Exchanging ATPase/metabolism ; Threonine/chemistry ; Threonine/genetics
Chemical Substances	Protein Isoforms ; Threonine (2ZD004190S) ; Aspartic Acid (30KYC7MIAI) ; Sodium-Potassium-Exchanging ATPase (EC 7.2.2.13)
Language	English
Publishing date	2008-07-25
Publishing country	Czech Republic
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1073141-6
ISSN	1802-9973 ; 0862-8408 ; 0369-9463
ISSN (online)	1802-9973
ISSN	0862-8408 ; 0369-9463
DOI	10.33549/physiolres.931469
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Article: Comparative modeling of the H4-H5-loop of the alpha2-isoform of Na+/K+-ATPase alpha-subunit in the E1 conformation.

Tejral, G / Kolácná, L / Kotyk, A / Amler, E

Physiological research

2007 Volume 56 Suppl 1, Page(s) S143–S151

Abstract: Restraint-based comparative modeling was used for calculation and visualization of the H4-H5-loop of Na+/K+-ATPase from mouse brain (Mus musculus, adult male brain, alpha2-isoform) between the amino acid residues Cys 336 and Arg 758 in the E1 ... ...

Abstract	Restraint-based comparative modeling was used for calculation and visualization of the H4-H5-loop of Na+/K+-ATPase from mouse brain (Mus musculus, adult male brain, alpha2-isoform) between the amino acid residues Cys 336 and Arg 758 in the E1 conformation The structure consists of two well separated parts. The N-domain is formed by a seven-stranded antiparallel beta-sheet with two additional beta-strands and five alpha-helices sandwiching it, the P-domain is composed of a typical Rossman fold. The ATP-binding site was found on the N-domain to be identical in both alpha2- and alpha1-isoforms. The phosphorylation Asp 369 residue was found in the central part of the P-domain, located at the C-terminal end of the central beta-sheet. The distance between the alpha-carbon of Phe 475 at the ATP-binding site and the alpha-carbon of Asp 369 at the phosphorylation site is 3.22 nm. A hydrogen bond between the oxygen atom of Asp 369 and the nitrogen atom of Lys 690 was clearly detected and assumed to play a key role in maintaining the proper structure of the phosphorylaton site in E1 conformation.
MeSH term(s)	Adenosine Triphosphate/metabolism ; Amino Acid Sequence ; Animals ; Binding Sites ; Brain/enzymology ; Computer Graphics ; Hydrogen Bonding ; Imaging, Three-Dimensional ; Isoenzymes/chemistry ; Male ; Mice ; Models, Molecular ; Molecular Sequence Data ; Phosphorylation ; Protein Conformation ; Protein Structure, Tertiary ; Reproducibility of Results ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sodium-Potassium-Exchanging ATPase/chemistry ; Sodium-Potassium-Exchanging ATPase/metabolism
Chemical Substances	Isoenzymes ; Adenosine Triphosphate (8L70Q75FXE) ; Atp1a2 protein, mouse (EC 3.6.1.-) ; Sodium-Potassium-Exchanging ATPase (EC 7.2.2.13)
Language	English
Publishing date	2007-05-31
Publishing country	Czech Republic
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1073141-6
ISSN	1802-9973 ; 0862-8408 ; 0369-9463
ISSN (online)	1802-9973
ISSN	0862-8408 ; 0369-9463
DOI	10.33549/physiolres.931313
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Article: Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix.

Kolácná, L / Bakesová, J / Varga, F / Kostáková, E / Plánka, L / Necas, A / Lukás, D / Amler, E / Pelouch, V

Physiological research

2007 Volume 56 Suppl 1, Page(s) S51–S60

Abstract: ECM is composed of different collagenous and non-collagenous proteins. Collagen nanofibers play a dominant role in maintaining the biological and structural integrity of various tissues and organs, including bone, skin, tendon, blood vessels, and ... ...

Abstract	ECM is composed of different collagenous and non-collagenous proteins. Collagen nanofibers play a dominant role in maintaining the biological and structural integrity of various tissues and organs, including bone, skin, tendon, blood vessels, and cartilage. Artificial collagen nanofibers are increasingly significant in numerous tissue engineering applications and seem to be ideal scaffolds for cell growth and proliferation. The modern tissue engineering task is to develop three-dimensional scaffolds of appropriate biological and biomechanical properties, at the same time mimicking the natural extracellular matrix and promoting tissue regeneration. Furthermore, it should be biodegradable, bioresorbable and non-inflammatory, should provide sufficient nutrient supply and have appropriate viscoelasticity and strength. Attributed to collagen features mentioned above, collagen fibers represent an obvious appropriate material for tissue engineering scaffolds. The aim of this minireview is, besides encapsulation of the basic biochemical and biophysical properties of collagen, to summarize the most promising modern methods and technologies for production of collagen nanofibers and scaffolds for artificial tissue development.
MeSH term(s)	Animals ; Collagen/chemistry ; Cytoskeleton/chemistry ; Elasticity ; Extracellular Matrix/chemistry ; Extracellular Matrix Proteins/chemistry ; Humans ; Materials Testing ; Nanostructures ; Stress, Mechanical ; Tissue Engineering ; Tissue Scaffolds ; Viscosity
Chemical Substances	Extracellular Matrix Proteins ; Collagen (9007-34-5)
Language	English
Publishing date	2007-05-31
Publishing country	Czech Republic
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1073141-6
ISSN	1802-9973 ; 0862-8408 ; 0369-9463
ISSN (online)	1802-9973
ISSN	0862-8408 ; 0369-9463
DOI	10.33549/physiolres.931302
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Article: Protein structure and dynamics determined by protein modeling combined with spectroscopic techniques.

Lansky, Z / Kubala, M / Varga, F / Kolacna, L / Amler, E

Bratislavske lekarske listy

2006 Volume 107, Issue 4, Page(s) 118–122

Abstract: Beside of the protein crystals, another attractive option in protein structure analysis has recently appeared: computer modeling of the protein structure based on homology and similarity with proteins of already known structures. We used the combination ... ...

Abstract	Beside of the protein crystals, another attractive option in protein structure analysis has recently appeared: computer modeling of the protein structure based on homology and similarity with proteins of already known structures. We used the combination of computer modeling with spectroscopic techniques, such as steady-state or time-resolved fluorescence spectroscopy or Raman spectroscopy, and with molecular biology techniques. This method could achieve reliable results comparable with resolution obtained from crystal structures. Molecular modeling of the ATP site within the H4-H5-loop revealed eight amino acids residues, namely besides the previously reported amino acids Asp443, Lys480, Lys501, Gly502 and Arg544, also Glu446, Phe475 and Gln482, which form the complete ATP recognition site. Moreover, we proved that a hydrogen bond between Arg423 and Glu472 supported the connection of two opposite halves of the ATP-binding pocket. Similarly, the conserved residue Pro489 is important for the proper interaction of the third and fourth-strands, which both contain residues that take part in the ATP-binding (Ref. 34).
MeSH term(s)	Adenosine Triphosphate/metabolism ; Binding Sites ; Computer Simulation ; Protein Conformation ; Sodium-Potassium-Exchanging ATPase/metabolism ; Spectrometry, Fluorescence ; Spectrum Analysis, Raman
Chemical Substances	Adenosine Triphosphate (8L70Q75FXE) ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)
Language	English
Publishing date	2006
Publishing country	Slovakia
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	127421-1
ISSN	0006-9248
ISSN	0006-9248
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Article: Fluorescent advanced glycation end products in the detection of factual stages of cartilage degeneration.

Handl, M / Filová, E / Kubala, M / Lánský, Z / Kolácná, L / Vorlícek, J / Trc, T / Pach, M / Amler, E

Physiological research

2006 Volume 56, Issue 2, Page(s) 235–242

Abstract: Patients treated for knee disorders were included in this study. They were examined clinically (Lequesne and Tegner scores) and by standard X-ray investigation. Patients underwent a surgical procedure, either arthroscopy or knee replacement. At the ... ...

Abstract	Patients treated for knee disorders were included in this study. They were examined clinically (Lequesne and Tegner scores) and by standard X-ray investigation. Patients underwent a surgical procedure, either arthroscopy or knee replacement. At the initial phase of surgery, a sample of cartilage was taken for laboratory examination. Progression of the disorder and the clinical examination was correlated with the actual state of the cartilage using a novel fluorescence approach. The intrinsic fluorescence of cartilages was shown as a suitable and sensitive method for detection of the actual state of cartilages because the correlation with X-ray examination and clinical status was found. Intrinsic fluorescence properties of cartilages from patients with chondropathy and osteoarthritis were described and found to be age-dependent. We also observed a higher concentration of advanced glycation end products due to inflammatory and/or degenerative processes in the cartilage. In addition, acute pathological changes due to diseases such as meniscal lesions or anterior cruciate ligament rupture caused a significant increase of formation of advanced glycation end products even in the group of young patients. In fact, such an observation could be crucial and important for the detection of knee conditions suspected of early meniscal and/or ACL lesions especially among young patients.
MeSH term(s)	Adolescent ; Adult ; Age Distribution ; Age Factors ; Aged ; Aged, 80 and over ; Arthroplasty, Replacement, Knee ; Arthroscopy ; Cartilage Diseases/diagnosis ; Cartilage Diseases/diagnostic imaging ; Cartilage Diseases/metabolism ; Cartilage Diseases/surgery ; Cartilage, Articular/diagnostic imaging ; Cartilage, Articular/metabolism ; Cartilage, Articular/surgery ; Female ; Glycation End Products, Advanced/metabolism ; Humans ; Joint Diseases/diagnosis ; Joint Diseases/diagnostic imaging ; Joint Diseases/metabolism ; Joint Diseases/surgery ; Knee Injuries/diagnosis ; Knee Injuries/diagnostic imaging ; Knee Injuries/metabolism ; Knee Injuries/surgery ; Knee Joint/diagnostic imaging ; Knee Joint/metabolism ; Knee Joint/surgery ; Male ; Middle Aged ; Osteoarthritis, Knee/diagnosis ; Osteoarthritis, Knee/metabolism ; Predictive Value of Tests ; Radiography ; Sensitivity and Specificity ; Severity of Illness Index ; Spectrometry, Fluorescence ; Synovitis/diagnosis ; Synovitis/metabolism
Chemical Substances	Glycation End Products, Advanced
Language	English
Publishing date	2006-03-23
Publishing country	Czech Republic
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1073141-6
ISSN	1802-9973 ; 0862-8408 ; 0369-9463
ISSN (online)	1802-9973
ISSN	0862-8408 ; 0369-9463
DOI	10.33549/physiolres.930934
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Article ; Online: Fibrin/hyaluronic acid composite hydrogels as appropriate scaffolds for in vivo artificial cartilage implantation.

Rampichová, Michala / Filová, Eva / Varga, Ferdinand / Lytvynets, Andriy / Prosecká, Eva / Koláčná, Lucie / Motlík, Jan / Nečas, Alois / Vajner, Luděk / Uhlík, Jiří / Amler, Evžen

ASAIO journal (American Society for Artificial Internal Organs : 1992)

2010 Volume 56, Issue 6, Page(s) 563–568

Abstract: Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation ... ...

Abstract	Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation of biomechanically stable tissue. Regenerated cartilage showed very good biomechanical and histological properties only 6 months after implantation. Notably, the quality of the healing process was dependent on the initial chondrocyte concentration of the scaffolds. These experiments were performed according to good laboratory practice (GLP).
MeSH term(s)	Animals ; Biocompatible Materials/chemistry ; Biomechanical Phenomena ; Cartilage/physiology ; Cartilage/surgery ; Chondrocytes/cytology ; Chondrocytes/physiology ; Chondrogenesis ; Fibrin/chemistry ; Hyaluronic Acid/chemistry ; Hydrogels ; Male ; Materials Testing ; Prostheses and Implants ; Regeneration ; Swine ; Swine, Miniature ; Tissue Engineering ; Tissue Scaffolds/chemistry
Chemical Substances	Biocompatible Materials ; Hydrogels ; Fibrin (9001-31-4) ; Hyaluronic Acid (9004-61-9)
Language	English
Publishing date	2010-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	759982-1
ISSN	1538-943X ; 0162-1432 ; 1058-2916
ISSN (online)	1538-943X
ISSN	0162-1432 ; 1058-2916
DOI	10.1097/MAT.0b013e3181fcbe24
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Article: Functional expression of the voltage-gated neuronal mammalian potassium channel rat ether à go-go1 in yeast.

Schwarzer, Sarah / Kolacna, Lucie / Lichtenberg-Fraté, Hella / Sychrova, Hana / Ludwig, Jost

FEMS yeast research

2008 Volume 8, Issue 3, Page(s) 405–413

Abstract: It has been shown previously that heterologous expression of inwardly rectifying potassium channels (K+-channels) from plants and mammals in K+-transport defective yeast mutants can restore the ability of growth in media with low [K+]. In this study, the ...

Abstract	It has been shown previously that heterologous expression of inwardly rectifying potassium channels (K+-channels) from plants and mammals in K+-transport defective yeast mutants can restore the ability of growth in media with low [K+]. In this study, the functional expression of an outward rectifying mammalian K+-channel in yeast is presented for the first time. The outward-rectifying mammalian neuronal K+-channel rat ether à go-go channel 1 (rEAG1, Kv 10.1) was expressed in yeast (Saccharomyces cerevisiae) strains lacking the endogenous K+-uptake systems and/or alkali-metal-cation efflux systems. It was found that a truncated channel version, lacking almost the complete intracellular N-terminus (rEAG1 Delta 190) but not the full-length rEAG1, partially complemented the growth defect of K+-uptake mutant cells (trk1,2 Delta tok1 Delta) in media containing low K+ concentrations. The expression of rEAG1 Delta 190 in a strain lacking the cation efflux systems (nha1 Delta ena1-4 Delta) increased the sensitivity to high monovalent cation concentrations. Both phenotypes were observed, when rEAG1 Delta 190 was expressed in a trk1,2 Delta and nha1, ena1-4 Delta mutant strain. In the presence of K+-channel blockers (Cs+, Ba2+ and quinidine), the growth advantage of rEAG1 Delta 190 expressing trk1,2 tok1 Delta cells disappeared, indicating its dependence on functional rEAG1 channels. The results demonstrate that S. cerevisiae is a suitable expression system even for voltage-gated outward-rectifying mammalian K+-channels.
MeSH term(s)	Animals ; Barium Compounds/pharmacology ; Chlorides/pharmacology ; Ether-A-Go-Go Potassium Channels/genetics ; Ether-A-Go-Go Potassium Channels/physiology ; Hydrogen-Ion Concentration ; Potassium/metabolism ; Potassium Channel Blockers/pharmacology ; Rats ; Recombinant Proteins/metabolism ; Rubidium/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism
Chemical Substances	Barium Compounds ; Chlorides ; Ether-A-Go-Go Potassium Channels ; Potassium Channel Blockers ; Recombinant Proteins ; barium chloride (0VK51DA1T2) ; Rubidium (MLT4718TJW) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2008-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2036775-2
ISSN	1567-1364 ; 1567-1356
ISSN (online)	1567-1364
ISSN	1567-1356
DOI	10.1111/j.1567-1364.2007.00351.x
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