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  1. Article ; Online: The fully automated Sysmex XN-31 hematology analyzer can detect bloodstream form Trypanosoma brucei.

    Khartabil, Tania / van Schaik, Ron Hn / Haanstra, Jurgen R / Koelewijn, Rob / Russcher, Henk / van Hellemond, Jaap J

    Diagnostic microbiology and infectious disease

    2024  Volume 108, Issue 4, Page(s) 116193

    Abstract: Background: For fully automated detection and quantification of Plasmodium parasites, Sysmex developed the XN-31 hemocytometer. This study investigated whether the XN-31 can also detect and quantify bloodstream form trypanosomes (trypomastigotes).: ... ...

    Abstract Background: For fully automated detection and quantification of Plasmodium parasites, Sysmex developed the XN-31 hemocytometer. This study investigated whether the XN-31 can also detect and quantify bloodstream form trypanosomes (trypomastigotes).
    Methods: Axenic cultures of Trypanosoma brucei brucei were used to prepare two dilution series of trypomastigotes in the whole blood of a healthy donor, which were subsequently examined by the XN-31 as well as by microscopic examination of thin and thick blood films. Trypomastigote intactness during the procedures was evaluated by microscopy.
    Results: The XN-31 hemocytometer detected trypomastigotes with a detection limit of 26 trypomastigotes/μL. Scattergram patterns of Trypanosoma and Plasmodium parasites were clearly distinct, but current interpretation settings do not allow the identification of trypomastigotes yet, and therefore, need future refinement.
    Conclusion: Proof of concept was provided for an automated fluorescent flow cytometry method that can detect and quantify Plasmodium spp., as well as Trypanosoma brucei trypomastigotes.
    MeSH term(s) Humans ; Trypanosoma brucei brucei ; Hematology/methods ; Reproducibility of Results ; Microscopy
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2024.116193
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  2. Article ; Online: Identification of gaps in the performance of routine microscopy for the diagnosis of parasitic infections revealed by the Dutch laboratory quality assessment scheme.

    Boonstra, Marrit B / Koelewijn, Rob / Brienen, Eric A T / Tassche-Borggreve, Kim / Kortbeek, Laetitia M / Mank, Theo G / Mulder, Bert / Stelma, Foekje / van Lieshout, Lisette / van Hellemond, Jaap J

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2024  

    Language English
    Publishing date 2024-02-23
    Publishing country England
    Document type Letter
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2024.02.018
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  3. Article ; Online: The decrease in plasma cholesterol during Plasmodium falciparum infections is not caused by cholesterol utilization by the parasites but by an infection-induced acute-phase response.

    Vliegenthart-Jongbloed, Klaske J / Koelewijn, Rob / Tielens, Aloysius G M / Sauerwein, Robert W / van Hellemond, Jaap J / van Genderen, Perry J J

    The Journal of infection

    2023  Volume 86, Issue 6, Page(s) 617–619

    MeSH term(s) Animals ; Humans ; Plasmodium falciparum ; Parasites ; Acute-Phase Reaction ; Malaria, Falciparum ; Infections ; Cholesterol
    Chemical Substances Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2023-03-02
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2023.02.031
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  4. Article ; Online: Influence of a chronic Schistosoma mansoni infection on the outcomes of a SARS-CoV-2 infection in the hamster model.

    Rissmann, Melanie / Veldhuis Kroeze, Edwin J B / Tielens, Aloysius G M / Rockx, Barry / van Hellemond, Jaap J

    The Journal of infection

    2023  Volume 87, Issue 3, Page(s) 273–276

    MeSH term(s) Cricetinae ; Animals ; Humans ; Schistosomiasis mansoni/complications ; COVID-19 ; SARS-CoV-2
    Language English
    Publishing date 2023-07-05
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2023.07.002
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  5. Article ; Online: Risk factors for acute toxoplasmosis in the Netherlands.

    Friesema, Ingrid H M / Hofhuis, Agnetha / Hoek-van Deursen, Denise / Jansz, Arjan R / Ott, Alewijn / van Hellemond, Jaap J / van der Giessen, Joke / Kortbeek, Laetitia M / Opsteegh, Marieke

    Epidemiology and infection

    2023  Volume 151, Page(s) e95

    Abstract: Toxoplasmosis caused by the protozoan ... ...

    Abstract Toxoplasmosis caused by the protozoan parasite
    MeSH term(s) Pregnancy ; Female ; Humans ; Netherlands/epidemiology ; Case-Control Studies ; Toxoplasmosis/epidemiology ; Toxoplasma ; Risk Factors
    Language English
    Publishing date 2023-05-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632982-2
    ISSN 1469-4409 ; 0950-2688
    ISSN (online) 1469-4409
    ISSN 0950-2688
    DOI 10.1017/S0950268823000808
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  6. Article ; Online: The unusual kinetics of lactate dehydrogenase of Schistosoma mansoni and their role in the rapid metabolic switch after penetration of the mammalian host.

    Bexkens, Michiel L / Martin, Olivier M F / van den Heuvel, Jos M / Schmitz, Marion G J / Teusink, Bas / Bakker, Barbara M / van Hellemond, Jaap J / Haanstra, Jurgen R / Walkinshaw, Malcolm D / Tielens, Aloysius G M

    International journal for parasitology

    2024  

    Abstract: Lactate dehydrogenase (LDH) from Schistosoma mansoni has peculiar properties for a eukaryotic LDH. Schistosomal LDH (SmLDH) isolated from schistosomes, and the recombinantly expressed protein, are strongly inhibited by ATP, which is neutralized by ... ...

    Abstract Lactate dehydrogenase (LDH) from Schistosoma mansoni has peculiar properties for a eukaryotic LDH. Schistosomal LDH (SmLDH) isolated from schistosomes, and the recombinantly expressed protein, are strongly inhibited by ATP, which is neutralized by fructose-1,6-bisphosphate (FBP). In the conserved FBP/anion binding site we identified two residues in SmLDH (Val187 and Tyr190) that differ from the conserved residues in LDHs of other eukaryotes, but are identical to conserved residues in FBP-sensitive prokaryotic LDHs. Three-dimensional (3D) models were generated to compare the structure of SmLDH with other LDHs. These models indicated that residues Val187, and especially Tyr190, play a crucial role in the interaction of FBP with the anion pocket of SmLDH. These 3D models of SmLDH are also consistent with a competitive model of SmLDH inhibition in which ATP (inhibitor) and FBP (activator) compete for binding in a well-defined anion pocket. The model of bound ATP predicts a distortion of the nearby key catalytic residue His195, resulting in enzyme inhibition. To investigate a possible physiological role of this allosteric regulation of LDH in schistosomes we made a kinetic model in which the allosteric regulation of the glycolytic enzymes can be varied. The model showed that inhibition of LDH by ATP prevents fermentation to lactate in the free-living stages in water and ensures complete oxidation via the Krebs cycle of the endogenous glycogen reserves. This mechanism of allosteric inhibition by ATP prevents the untimely depletion of these glycogen reserves, the only fuel of the free-living cercariae. Neutralization by FBP of this ATP inhibition of LDH prevents accumulation of glycolytic intermediates when S. mansoni schistosomula are confronted with the sudden large increase in glucose availability upon penetration of the final host. It appears that the LDH of S. mansoni is special and well suited to deal with the variations in glucose availability the parasite encounters during its life cycle.
    Language English
    Publishing date 2024-03-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 120518-3
    ISSN 1879-0135 ; 0020-7519
    ISSN (online) 1879-0135
    ISSN 0020-7519
    DOI 10.1016/j.ijpara.2024.03.005
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    Zs.A 789: Show issues Location:
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  7. Article ; Online: Fast detection and quantification of Plasmodium species infected erythrocytes in a non-endemic region by using the Sysmex XN-31 analyzer.

    Khartabil, Tania A / de Rijke, Yolanda B / Koelewijn, Rob / van Hellemond, Jaap J / Russcher, Henk

    Malaria journal

    2022  Volume 21, Issue 1, Page(s) 119

    Abstract: Background: Due to increased travel from endemic countries, malaria occurs more frequently in non-endemic regions. It is a challenge for diagnostic laboratories in non-endemic countries to provide reliable results, as experience of staff is often ... ...

    Abstract Background: Due to increased travel from endemic countries, malaria occurs more frequently in non-endemic regions. It is a challenge for diagnostic laboratories in non-endemic countries to provide reliable results, as experience of staff is often limited to only a few cases per year. This study evaluated the diagnostic accuracy of the fully automated Sysmex XN-31 malaria analyzer in a routine diagnostic setting in a non-endemic region was evaluated.
    Methods: Samples from 112 patients suspected for malaria were examined by the Sysmex XN-31 analyzer to determine the absolute count of malaria-infected red blood cells count (MI-RBC/µL). Microscopic examination of both Quantitative Buffy Coat capillary tubes and thick and thin blood films were used as reference methods. Limits of blank (LoB), detection (LoD) and quantification (LoQ) were investigated using an in vitro Plasmodium falciparum culture. Nine hundred twenty samples of patients with RBC abnormalities were included to determine which RBC abnormalities trigger indeterminate or false positive results.
    Results: No false positive nor false negative results were obtained for the examined patient samples suspected for malaria. For 3% of samples an indeterminate result by the XN-31 was obtained. The Passing-Bablok regression line for diagnostic accuracy of the parasitaemia was y = 39.75 + 0.7892 × showing a positive bias of about 21% when comparing the MI-RBC results to microscopy. The LoB, LoD and LoQ were calculated to be 4.7, 5.9, and 19.0 infected RBC/μL, respectively. From the 920 abnormal RBC samples collected, 4.6% resulted in a false positive MI-RBC result and almost half of the samples produced indeterminate results. These results were related to increases in nucleated red blood cells, reticulocytes and other abnormal RBC morphologies such as sickle cells.
    Conclusions: Based on the results, the XN-31 is a fast and reliable screening method in the detection and quantification of Plasmodium species in patients However, if an abnormal red blood cell morphology is present, the results of the XN-31 should be interpreted with caution as false positive results can be caused by interfering abnormal erythrocytes.
    MeSH term(s) Erythrocytes ; Humans ; Malaria/diagnosis ; Parasitemia/diagnosis ; Plasmodium ; Plasmodium falciparum
    Language English
    Publishing date 2022-04-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091229-8
    ISSN 1475-2875 ; 1475-2875
    ISSN (online) 1475-2875
    ISSN 1475-2875
    DOI 10.1186/s12936-022-04147-0
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  8. Article ; Online: Single-dose pentamidine substantially reduces viability of trypanosomes in human East African trypanosomiasis.

    van Genderen, Perry J J / Nouwen, Jan L / De Mendonça Melo, Mariana / Rijnders, Bart J A / van Hellemond, Jaap J

    Journal of travel medicine

    2021  Volume 28, Issue 6

    MeSH term(s) Animals ; Humans ; Pentamidine ; Trypanocidal Agents/therapeutic use ; Trypanosoma ; Trypanosomiasis, African/drug therapy
    Chemical Substances Trypanocidal Agents ; Pentamidine (673LC5J4LQ)
    Language English
    Publishing date 2021-05-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 1212504-0
    ISSN 1708-8305 ; 1195-1982
    ISSN (online) 1708-8305
    ISSN 1195-1982
    DOI 10.1093/jtm/taab080
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  9. Article ; Online: Are humanized IgE reporter systems potential game changers in serological diagnosis of human parasitic infection?

    Prakash, Prema S / Weber, Michael H W / van Hellemond, Jaap J / Falcone, Franco H

    Parasitology research

    2021  Volume 121, Issue 4, Page(s) 1137–1144

    Abstract: Immunoglobulin E (IgE) is thought to have evolved to protect mammalian hosts against parasitic infections or toxins and plays a central role in the pathogenesis, diagnosis, and therapy of IgE-mediated allergy. Despite the prominence of IgE responses in ... ...

    Abstract Immunoglobulin E (IgE) is thought to have evolved to protect mammalian hosts against parasitic infections or toxins and plays a central role in the pathogenesis, diagnosis, and therapy of IgE-mediated allergy. Despite the prominence of IgE responses in most parasitic infections, and in stark contrast to its use in the diagnosis of allergy, this isotype is almost completely unexploited for parasite diagnosis. Here, we discuss the perceived or real limitations of IgE-based diagnosis in parasitology and suggest that the recent creation of a new generation of very sensitive cellular IgE-based reporters may represent a powerful new diagnostic platform, but needs to be based on a very careful choice of diagnostic allergens.
    MeSH term(s) Allergens ; Animals ; Humans ; Hypersensitivity/diagnosis ; Immunoglobulin E ; Mammals ; Parasitic Diseases/diagnosis
    Chemical Substances Allergens ; Immunoglobulin E (37341-29-0)
    Language English
    Publishing date 2021-11-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-021-07352-z
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    Uh II Zs.124: Show issues Location:
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  10. Article ; Online: Inhibition of Fatty Acid Oxidation as a New Target To Treat Primary Amoebic Meningoencephalitis.

    Sarink, Maarten J / Tielens, Aloysius G M / Verbon, Annelies / Sutak, Robert / van Hellemond, Jaap J

    Antimicrobial agents and chemotherapy

    2020  Volume 64, Issue 8

    Abstract: Primary amoebic meningoencephalitis (PAM) is a rapidly fatal infection caused by the free-living ... ...

    Abstract Primary amoebic meningoencephalitis (PAM) is a rapidly fatal infection caused by the free-living amoeba
    MeSH term(s) Amphotericin B ; Animals ; Central Nervous System Protozoal Infections ; Fatty Acids ; Meningoencephalitis ; Naegleria ; Naegleria fowleri
    Chemical Substances Fatty Acids ; Amphotericin B (7XU7A7DROE)
    Language English
    Publishing date 2020-07-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/AAC.00344-20
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