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  1. Article ; Online: An AIE and ESIPT based neuraminidase fluorescent probe for influenza virus detection and imaging.

    Chang, Hao / Mei, Yu / Li, Yidan / Shang, Luqing

    Talanta

    2022  Volume 247, Page(s) 123583

    Abstract: Neuraminidase (NA), an important enzyme for influenza virus replication, is a main target for influenza virus inhibition and detection. In this work, the aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT)-active ... ...

    Abstract Neuraminidase (NA), an important enzyme for influenza virus replication, is a main target for influenza virus inhibition and detection. In this work, the aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT)-active neuraminidase fluorescent probe SABP was firstly developed for influenza virus detection. The probe SABP showed high sensitivity towards NA with strong green fluorescence, which could be used for influenza virus detection and living cell imaging. In the presence of NA, SABP exhibited increased fluorescence signal by up to 30-fold at 524 nm. SABP detected NA activity in the range 0-5.0 U/mL, with a limit of detection (LOD) of 0.024 U/mL and detect influenza virus in the range of 2
    MeSH term(s) Antiviral Agents/pharmacology ; Enzyme Inhibitors/pharmacology ; Fluorescent Dyes/pharmacology ; Humans ; Influenza, Human/diagnosis ; Neuraminidase ; Orthomyxoviridae ; Protons
    Chemical Substances Antiviral Agents ; Enzyme Inhibitors ; Fluorescent Dyes ; Protons ; Neuraminidase (EC 3.2.1.18)
    Language English
    Publishing date 2022-05-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2022.123583
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Mitochondria-Targeted Fluorescent Probe for Monitoring NADPH Overproduction during Influenza Virus Infection.

    Chang, Hao / Hu, Xiao / Tang, Xiaomei / Tian, Shiwei / Li, Yidan / Lv, Xing / Shang, Luqing

    ACS sensors

    2023  Volume 8, Issue 2, Page(s) 829–838

    Abstract: Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor in the progress of antioxidant synthesis and biosynthesis, and an abnormal NADPH level has been observed in many viral infection processes. However, efficient tools to ... ...

    Abstract Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor in the progress of antioxidant synthesis and biosynthesis, and an abnormal NADPH level has been observed in many viral infection processes. However, efficient tools to monitor NADPH in living cells after viral infection have not been reported. In this work, we present a fluorescent probe,
    MeSH term(s) Humans ; NADP/metabolism ; Fluorescent Dyes ; Influenza, Human ; Mitochondria/metabolism ; Orthomyxoviridae/metabolism
    Chemical Substances NADP (53-59-8) ; Fluorescent Dyes
    Language English
    Publishing date 2023-01-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2379-3694
    ISSN (online) 2379-3694
    DOI 10.1021/acssensors.2c02458
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: An AIE and ESIPT based neuraminidase fluorescent probe for influenza virus detection and imaging

    Chang, Hao / Mei, Yu / Li, Yidan / Shang, Luqing

    Talanta. 2022 Sept. 01, v. 247

    2022  

    Abstract: Neuraminidase (NA), an important enzyme for influenza virus replication, is a main target for influenza virus inhibition and detection. In this work, the aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT)-active ... ...

    Abstract Neuraminidase (NA), an important enzyme for influenza virus replication, is a main target for influenza virus inhibition and detection. In this work, the aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT)-active neuraminidase fluorescent probe SABP was firstly developed for influenza virus detection. The probe SABP showed high sensitivity towards NA with strong green fluorescence, which could be used for influenza virus detection and living cell imaging. In the presence of NA, SABP exhibited increased fluorescence signal by up to 30-fold at 524 nm. SABP detected NA activity in the range 0–5.0 U/mL, with a limit of detection (LOD) of 0.024 U/mL and detect influenza virus in the range of 2⁻⁴–2⁶ HAU with a LOD of 2⁻¹ HAU. Moreover, SABP was successfully applied to distinguish oseltamivir-resistant influenza virus from wild type.
    Keywords Orthomyxoviridae ; detection limit ; fluorescence ; fluorescent dyes ; sialidase ; virus replication
    Language English
    Dates of publication 2022-0901
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2022.123583
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Discovery of SARS-CoV-2 3CL

    Wang, Yaxin / Xu, Binghong / Ma, Sen / Wang, Hao / Shang, Luqing / Zhu, Cheng / Ye, Sheng

    International journal of molecular sciences

    2022  Volume 23, Issue 4

    Abstract: As the etiological agent for the coronavirus disease 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenges the ongoing efforts of vaccine development and drug design. Due to the accumulating cases of breakthrough infections, there ...

    Abstract As the etiological agent for the coronavirus disease 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenges the ongoing efforts of vaccine development and drug design. Due to the accumulating cases of breakthrough infections, there are urgent needs for broad-spectrum antiviral medicines. Here, we designed and examined five new tetrapeptidomimetic anti-SARS-CoV-2 inhibitors targeting the 3C-Like protease (3CL
    MeSH term(s) Animals ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; COVID-19/drug therapy ; Catalytic Domain ; Chlorocebus aethiops ; Coronavirus 3C Proteases/antagonists & inhibitors ; Coronavirus 3C Proteases/chemistry ; Drug Design ; Fluorescence Resonance Energy Transfer ; Histidine/chemistry ; Ligands ; Molecular Dynamics Simulation ; Peptidomimetics/chemistry ; Peptidomimetics/pharmacology ; Protease Inhibitors/chemistry ; Protease Inhibitors/pharmacology ; SARS-CoV-2/drug effects ; Structure-Activity Relationship ; Vero Cells
    Chemical Substances Antiviral Agents ; Ligands ; Peptidomimetics ; Protease Inhibitors ; Histidine (4QD397987E) ; 3C-like proteinase, SARS-CoV-2 (EC 3.4.22.-) ; Coronavirus 3C Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2022-02-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23042392
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Discovery of SARS-CoV-2 3CL Pro Peptidomimetic Inhibitors through the Catalytic Dyad Histidine-Specific Protein–Ligand Interactions

    Yaxin Wang / Binghong Xu / Sen Ma / Hao Wang / Luqing Shang / Cheng Zhu / Sheng Ye

    International Journal of Molecular Sciences, Vol 23, Iss 2392, p

    2022  Volume 2392

    Abstract: As the etiological agent for the coronavirus disease 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenges the ongoing efforts of vaccine development and drug design. Due to the accumulating cases of breakthrough infections, there ...

    Abstract As the etiological agent for the coronavirus disease 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenges the ongoing efforts of vaccine development and drug design. Due to the accumulating cases of breakthrough infections, there are urgent needs for broad-spectrum antiviral medicines. Here, we designed and examined five new tetrapeptidomimetic anti-SARS-CoV-2 inhibitors targeting the 3C-Like protease (3CL Pro ), which is highly conserved among coronaviruses and essential for viral replications. We significantly improved the efficacy of a ketoamide lead compound based on high-resolution co-crystal structures, all-atom simulations, and binding energy calculations. The inhibitors successfully engaged the catalytic dyad histidine residue (H41) of 3CLPro as designed, and they exhibited nanomolar inhibitory capacity as well as mitigated the viral loads of SARS-CoV-2 in cellular assays. As a widely applicable design principle, our results revealed that the potencies of 3CL Pro -specific drug candidates were determined by the interplay between 3CL Pro H41 residue and the peptidomimetic inhibitors.
    Keywords SARS-CoV-2 ; covalent inhibitor ; molecular dynamics simulations ; molecule design ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  6. Article ; Online: Correction: TMEM158 promotes the proliferation and migration of glioma cells via STAT3 signaling in glioblastomas.

    Li, Jiabo / Wang, Xuya / Chen, Lulu / Zhang, Jinhao / Zhang, Yiming / Ren, Xiao / Sun, Jinzhang / Fan, Xiaoguang / Fan, Jikang / Li, Tao / Tong, Luqing / Yi, Li / Chen, Lei / Liu, Jie / Shang, Guanjie / Ren, Xiude / Zhang, Hao / Yu, Shengping / Ming, Haolang /
    Huang, Qiang / Dong, Jun / Zhang, Chen / Yang, Xuejun

    Cancer gene therapy

    2024  Volume 31, Issue 3, Page(s) 495–496

    Language English
    Publishing date 2024-01-11
    Publishing country England
    Document type Published Erratum
    ZDB-ID 1212513-1
    ISSN 1476-5500 ; 0929-1903
    ISSN (online) 1476-5500
    ISSN 0929-1903
    DOI 10.1038/s41417-024-00733-3
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4125: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Reversible covalent inhibitors suppress enterovirus 71 infection by targeting the 3C protease.

    Liu, Meijun / Xu, Binghong / Ma, Yuying / Shang, Luqing / Ye, Sheng / Wang, Yaxin

    Antiviral research

    2021  Volume 192, Page(s) 105102

    Abstract: As one of the principal etiological agents of hand, foot, and mouth disease (HFMD), enterovirus 71 (EV71) is associated with severe neurological complications or fatal diseases, while without effective medications thus far. Here we applied dually ... ...

    Abstract As one of the principal etiological agents of hand, foot, and mouth disease (HFMD), enterovirus 71 (EV71) is associated with severe neurological complications or fatal diseases, while without effective medications thus far. Here we applied dually activated Michael acceptor to develop a series of reversible covalent compounds for EV71 3C protease (3C
    MeSH term(s) 3C Viral Proteases/antagonists & inhibitors ; 3C Viral Proteases/chemistry ; Acrylamides/chemistry ; Acrylamides/pharmacology ; Animals ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Cell Line ; Cell Survival/drug effects ; Cyanoacrylates/chemistry ; Cyanoacrylates/pharmacology ; Enterovirus/classification ; Enterovirus/drug effects ; Enterovirus A, Human/drug effects ; Enterovirus Infections/virology ; Humans ; Molecular Docking Simulation ; Protease Inhibitors/chemistry ; Protease Inhibitors/pharmacology ; Virus Replication/drug effects
    Chemical Substances Acrylamides ; Antiviral Agents ; Cyanoacrylates ; Protease Inhibitors ; 3C Viral Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2021-06-01
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2021.105102
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1627: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: The Potential Mechanisms of Cinobufotalin Treating Colon Adenocarcinoma by Network Pharmacology.

    Wang, Jiyan / Chang, Hongkai / Su, Meng / Zhao, Huifang / Qiao, Yaya / Wang, Yu / Shang, Luqing / Shan, Changliang / Zhang, Shuai

    Frontiers in pharmacology

    2022  Volume 13, Page(s) 934729

    Abstract: Network pharmacology, as a novel way using bioinformatics to explore drug targets and interactions in cancer, broadens our understanding of drug action, thereby facilitating drug discovery. Here, we utilized network pharmacology to explore the role and ... ...

    Abstract Network pharmacology, as a novel way using bioinformatics to explore drug targets and interactions in cancer, broadens our understanding of drug action, thereby facilitating drug discovery. Here, we utilized network pharmacology to explore the role and mechanism by which cinobufotalin functions in colon adenocarcinoma (COAD). We found that cinobufotalin represses the growth and proliferation of colon cancer cells, and integrated public databases for targets reported to be associated with COAD, together with those predicted to be targets of cinobufotalin. Targets overlapped between COAD-associated proteins and cinobufotalin target proteins were used to filter candidate targets of cinobufotalin in COAD. The following proteins were thought to occupy a key position in COAD-cinobufotalin target networks: SRC, PIK3R1, MAPK1, PIK3CA, HSP90AA1, CTNNB1, GRB2, RHO1, PTPN11, and EGFR. The networks regulated by cinobufotalin were involved mainly in extracellular signal stimulation and transduction, including MAPK signaling pathway, PI3K-AKT signaling pathway, and JAK-STAT signaling pathway. Besides, transcriptome sequencing results also indicated that cinobufotalin inhibits the response of colon cancer cells to extracellular stimulation and promotes cell apoptosis. Molecular docking results showed that cinobufotalin matches in the pocket of the top candidate cinobufotalin target proteins (SRC, PIK3R1, MAPK1 and PIK3CA). These findings demonstrate cinobufotalin can be developed as potential anti-cancer therapeutics.
    Language English
    Publishing date 2022-06-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2022.934729
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: 4-Iminooxazolidin-2-One as a Bioisostere of Cyanohydrin Suppresses EV71 Proliferation by Targeting 3C

    Xu, Binghong / Liu, Meijun / Ma, Sen / Ma, Yuying / Liu, Si / Shang, Luqing / Zhu, Cheng / Ye, Sheng / Wang, Yaxin

    Microbiology spectrum

    2021  Volume 9, Issue 3, Page(s) e0102521

    Abstract: The fatal pathogen enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease (HFMD), which leads to serious neurological syndromes. While there are no effective clinical agents available for EV71 treatment thus far, EV71 3C protease ( ... ...

    Abstract The fatal pathogen enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease (HFMD), which leads to serious neurological syndromes. While there are no effective clinical agents available for EV71 treatment thus far, EV71 3C protease (3C
    MeSH term(s) Animals ; Humans ; 3C Viral Proteases/antagonists & inhibitors ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Cell Line, Tumor ; Chlorocebus aethiops ; Enterovirus A, Human/drug effects ; Enterovirus A, Human/growth & development ; Hand, Foot and Mouth Disease/drug therapy ; Hand, Foot and Mouth Disease/prevention & control ; Hand, Foot and Mouth Disease/virology ; HEK293 Cells ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Nitriles/chemistry ; Nitriles/pharmacology ; Vero Cells ; Virus Replication/drug effects
    Chemical Substances 3C Viral Proteases (EC 3.4.22.28) ; Antiviral Agents ; cyanohydrin ; Nitriles ; 4-iminooxazolidin-2-one
    Language English
    Publishing date 2021-11-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/Spectrum.01025-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Design and application of near-infrared fluorophore based on a novel thiazolidinedione-functionalized dicyanoisophorone.

    Liang, Xiao / Zhang, Lu / Shi, Bing / Chang, Hao / Qiao, Dan / Shen, Tangliang / Zhao, Wei / Yin, Zheng / Shang, Luqing

    Talanta

    2020  Volume 220, Page(s) 121433

    Abstract: A novel dicyanoisophorone (DCI)-based NIR fluorophore employing 2, 4-thiazolidinediones as the modification site was designed for fluorescence imaging. The fluorophore was assessed as a switchable reporter for ... ...

    Abstract A novel dicyanoisophorone (DCI)-based NIR fluorophore employing 2, 4-thiazolidinediones as the modification site was designed for fluorescence imaging. The fluorophore was assessed as a switchable reporter for H
    Language English
    Publishing date 2020-07-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2020.121433
    Database MEDical Literature Analysis and Retrieval System OnLINE

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