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Article ; Online: m

Akhtar, Junaid / Lugoboni, Margot / Junion, Guillaume

2022 Volume 12, Issue 5, Page(s) 266–276

Abstract: ... of normal development and physiology. For example, m ...

Abstract	RNA modifications are prevalent among all the classes of RNA, regulate diverse biological processes, and have emerged as a key regulatory mechanism in post-transcriptional control of gene expression. They are subjected to precise spatial and temporal control and shown to be critical for the maintenance of normal development and physiology. For example, m
MeSH term(s)	Adenosine/genetics ; Adenosine/metabolism ; Gene Expression Regulation ; RNA/metabolism ; RNA Processing, Post-Transcriptional ; RNA Splicing ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Chemical Substances	RNA, Messenger ; RNA (63231-63-0) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2022-04-05
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2646974-1
ISSN	2154-1272 ; 2154-1264
ISSN (online)	2154-1272
ISSN	2154-1264
DOI	10.1080/21541264.2022.2057177
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Article ; Online: m

Akhtar, Junaid / Renaud, Yoan / Albrecht, Steffen / Ghavi-Helm, Yad / Roignant, Jean-Yves / Silies, Marion / Junion, Guillaume

Molecular cell

2021 Volume 81, Issue 16, Page(s) 3356–3367.e6

Abstract: ... critical for development of metazoans. Here, we show that the m ...

Abstract	RNA polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m
MeSH term(s)	Animals ; Drosophila Proteins/genetics ; Drosophila melanogaster/genetics ; Methyltransferases/genetics ; Multiprotein Complexes/genetics ; Nuclear Proteins/genetics ; Promoter Regions, Genetic/genetics ; RNA Polymerase II/genetics ; Transcription, Genetic
Chemical Substances	Drosophila Proteins ; Multiprotein Complexes ; Nuclear Proteins ; Ythdc1 protein, Drosophila ; Methyltransferases (EC 2.1.1.-) ; RNA Polymerase II (EC 2.7.7.-)
Language	English
Publishing date	2021-07-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1415236-8
ISSN	1097-4164 ; 1097-2765
ISSN (online)	1097-4164
ISSN	1097-2765
DOI	10.1016/j.molcel.2021.06.023
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Article ; Online: m

Lence, Tina / Akhtar, Junaid / Bayer, Marc / Schmid, Katharina / Spindler, Laura / Ho, Cheuk Hei / Kreim, Nastasja / Andrade-Navarro, Miguel A / Poeck, Burkhard / Helm, Mark / Roignant, Jean-Yves

Nature

2016 Volume 540, Issue 7632, Page(s) 242–247

Abstract: ... ...

Abstract	N
MeSH term(s)	Adenosine/analogs & derivatives ; Adenosine/metabolism ; Alternative Splicing ; Animals ; Behavior, Animal/physiology ; Drosophila Proteins/chemistry ; Drosophila Proteins/deficiency ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/cytology ; Drosophila melanogaster/enzymology ; Drosophila melanogaster/physiology ; Female ; Male ; Methyltransferases/chemistry ; Methyltransferases/metabolism ; Nervous System/metabolism ; Neurons/physiology ; Nuclear Proteins/chemistry ; Nuclear Proteins/deficiency ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Phenotype ; Protein Subunits/chemistry ; Protein Subunits/metabolism ; RNA-Binding Proteins/genetics ; Sex Determination Processes/genetics ; Sex Determination Processes/physiology
Chemical Substances	Drosophila Proteins ; NITO protein, Drosophila ; Nuclear Proteins ; Protein Subunits ; RNA-Binding Proteins ; Sxl protein, Drosophila ; Ythdc1 protein, Drosophila ; N-methyladenosine (CLE6G00625) ; Methyltransferases (EC 2.1.1.-) ; mRNA (2'-O-methyladenosine-N6-)-methyltransferase (EC 2.1.1.62) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2016-11-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/nature20568
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Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Development of multivalent vaccine targeting M segment of Crimean Congo Hemorrhagic Fever Virus (CCHFV) using immunoinformatic approaches.

Sana, Maaza / Javed, Aneela / Babar Jamal, Syed / Junaid, Muhammad / Faheem, Muhammad

Saudi journal of biological sciences

2021 Volume 29, Issue 4, Page(s) 2372–2388

Abstract: ... in M segment) of CCHFV with an N-terminus human β-defensin as an adjuvant, as well as an N-terminus ...

Abstract	Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne viral infection with no licensed vaccine or therapeutics available for its treatment. In the present study we have developed the first multi-epitope subunit vaccine effective against all the seven genotypes of CCHF virus (CCHFV). The vaccine contains five B-cell, two MHC-II (HTL), and three MHC-I (CTL) epitopes screened from two structural glycoproteins (Gc and Gn in M segment) of CCHFV with an N-terminus human β-defensin as an adjuvant, as well as an N-terminus EAAAK sequence. The epitopes were rigorously investigated for their antigenicity, allergenicity, IFN gamma induction, anti-inflammatory responses, stability, and toxicity. The three-dimensional structure of the vaccine was predicted and docked with TLR-3, TLR-8, and TLR-9 receptors to find the strength of the binding complexes via molecular dynamics simulation. After codon adaptation, the subunit vaccine construct was developed in a pDual-GC plasmid and has population coverage of 98.47% of the world's population (HLA-I & II combined). The immune simulation studies were carried out on the C-ImmSim in-silico interface showing a marked increase in the production of cellular and humoral response (B-cell and T-cell) as well as TGFβ, IL-2, IL-10, and IL-12 indicating that the proposed vaccine would be able to sufficiently provoke both humoral and cell-mediated immune responses. Thus, making it a new and promising vaccine candidate against CCHFV.
Language	English
Publishing date	2021-12-10
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2021.12.004
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Article: Multivariate Analysis Coupled with M-SVM Classification for Lard Adulteration Detection in Meat Mixtures of Beef, Lamb, and Chicken Using FTIR Spectroscopy.

Siddiqui, Muhammad Aadil / Khir, Mohd Haris Md / Witjaksono, Gunawan / Ghumman, Ali Shaan Manzoor / Junaid, Muhammad / Magsi, Saeed Ahmed / Saboor, Abdul

Foods (Basel, Switzerland)

2021 Volume 10, Issue 10

Abstract: Adulteration of meat products is a delicate issue for people around the globe. The mixing of lard in meat causes a significant problem for end users who are sensitive to halal meat consumption. Due to the highly similar lipid profiles of meat species, ... ...

Abstract	Adulteration of meat products is a delicate issue for people around the globe. The mixing of lard in meat causes a significant problem for end users who are sensitive to halal meat consumption. Due to the highly similar lipid profiles of meat species, the identification of adulteration becomes more difficult. Therefore, a comprehensive spectral detailing of meat species is required, which can boost the adulteration detection process. The experiment was conducted by distributing samples labeled as "Pure (80 samples)" and "Adulterated (90 samples)". Lard was mixed with the ratio of 10-50%
Language	English
Publishing date	2021-10-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods10102405
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Article ; Online: Molecular analysis of blaSHV, blaTEM, and blaCTX-M in extended-spectrum β-lactamase producing Enterobacteriaceae recovered from fecal specimens of animals.

Ejaz, Hasan / Younas, Sonia / Abosalif, Khalid O A / Junaid, Kashaf / Alzahrani, Badr / Alsrhani, Abdullah / Abdalla, Abualgasim Elgaili / Ullah, Muhammad Ikram / Qamar, Muhammad Usman / Hamam, Sanaa S M

PloS one

2021 Volume 16, Issue 1, Page(s) e0245126

Abstract: ... of blaSHV, blaTEM, and blaCTX-M variants in animals, as well as to evaluate the antimicrobial resistance ... and blaCTX-M variants. The drug-resistance spectrum and hierarchical clusters were analyzed against 19 ... Klebsiella pneumoniae were prevalent species. A total of 73.9% blaCTX-M, 26.1% blaTEM, and 14.2% blaSHV were found ...

Abstract	Colonization of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as animal gut microbiota is a substantial global threat. This study aimed to determine the molecular characterization of blaSHV, blaTEM, and blaCTX-M variants in animals, as well as to evaluate the antimicrobial resistance conferred by these genes. We prospectively analyzed 1273 fecal specimens of farm and domestic animals for the isolation of enterobacteria that had the ESBL phenotype by using biochemical methods. The extracted genes were amplified by polymerase chain reaction and sequenced for the characterization of blaSHV, blaTEM, and blaCTX-M variants. The drug-resistance spectrum and hierarchical clusters were analyzed against 19 antibacterial agents. Out of 245 (19.2%) ESBL enterobacteria, 180 (75.5%) Escherichia coli and 34 (13.9%) Klebsiella pneumoniae were prevalent species. A total of 73.9% blaCTX-M, 26.1% blaTEM, and 14.2% blaSHV were found among the enterobacteria; however, their association with farm or domestic animals was not statistically significant. The distribution of bla gene variants showed the highest number of blaCTX-M-1 (133; 54.3%), followed by blaCTX-M-15 (28; 11.4%), blaTEM-52 (40; 16.3%), and blaSHV-12 (22; 9%). In addition, 84.5% of the enterobacteria had the integrons intI1. We observed ±100% enterobacteria resistant to cephalosporin, 7 (2.9%) to colistin (minimum inhibitory concentration breakpoint ≥4 μg/mL), 9 (3.7%) to piperacillin-tazobactam, 11 (4.5%) to imipenem, 14 (5.7%) to meropenem, and 18 (7.3%) to cefoperazone-sulbactam, without statistically significant association. Animal gut microbiota contain a considerable number of blaCTX-M, blaTEM, blaSHV, and integrons, which are a potential source of acquired extensive drug resistance in human strains and leaves fewer therapeutic substitutes.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Escherichia coli/classification ; Escherichia coli/enzymology ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Feces/microbiology ; Gastrointestinal Microbiome ; Humans ; Klebsiella pneumoniae/classification ; Klebsiella pneumoniae/enzymology ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/isolation & purification ; beta-Lactam Resistance/genetics ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2021-01-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0245126
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Article: Facilely Synthesized M-Montmorillonite (M = Cr, Fe, and Co) as Efficient Catalysts for Enhancing CO₂ Desorption from Amine Solution

Bhatti, Umair H. / Kazmi, Wajahat W. / Min, Gwan Hong / Haider, Junaid / Nam, Sungchan / Baek, Il Hyun

Industrial & engineering chemistry process design and development. 2021 Sept. 01, v. 60, no. 36

2021

Abstract: ... desorption at low temperatures. In this work, we synthesized inexpensive M-montmorillonite (M = Cr, Fe, and ...

Abstract	Catalytic amine regeneration has recently emerged as an effective strategy to improve CO₂ desorption at low temperatures. In this work, we synthesized inexpensive M-montmorillonite (M = Cr, Fe, and Co) catalysts via a facile metal ion-exchange process and used these to optimize the CO₂ desorption rate of a 30 wt % monoethanolamine (MEA) solution at a moderate temperature (∼86 °C). The metal ion-exchange process led to Si and Al leaching from the aluminosilicate layers and cation removal from the Mont interlayers, resulting in an increase in the surface acidity, mesoporosity, and total surface area of the ion-exchanged Mont catalysts. The prepared catalysts introduce acid sites to amine solution that can attach with the carbamate, carbonate, and bicarbonates, to favor the CO₂ desorption at low temperatures. Overall, the CO₂ desorption rate and the total amount of released CO₂ were improved up to 315 and 82.5%, respectively, whereas the regeneration energy penalty was reduced by 40%, in comparison with the noncatalytic MEA solution. The impact of various physicochemical catalytic properties on the CO₂ desorption performance was also evaluated. The stability of the prepared catalysts was verified in five cyclic uses and no change in the catalytic activity or structure was detected. In addition, the catalysts were readily separable by simple filtration. This work introduces an effective strategy to design abundant and cost-effective catalysts for energy-efficient CO₂ capture.
Keywords	acidity ; aluminum silicates ; carbon dioxide ; carbonates ; catalytic activity ; cations ; cost effectiveness ; desorption ; energy efficiency ; ethanolamine ; filtration ; ion exchange ; process design ; surface area ; temperature
Language	English
Dates of publication	2021-0901
Size	p. 13318-13325.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1484436-9
ISSN	1520-5045 ; 0888-5885
ISSN (online)	1520-5045
ISSN	0888-5885
DOI	10.1021/acs.iecr.1c02487
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Article ; Online: Molecular analysis of blaSHV, blaTEM, and blaCTX-M in extended-spectrum β-lactamase producing Enterobacteriaceae recovered from fecal specimens of animals.

Hasan Ejaz / Sonia Younas / Khalid O A Abosalif / Kashaf Junaid / Badr Alzahrani / Abdullah Alsrhani / Abualgasim Elgaili Abdalla / Muhammad Ikram Ullah / Muhammad Usman Qamar / Sanaa S M Hamam

PLoS ONE, Vol 16, Iss 1, p e

2021 Volume 0245126

Abstract	Colonization of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as animal gut microbiota is a substantial global threat. This study aimed to determine the molecular characterization of blaSHV, blaTEM, and blaCTX-M variants in animals, as well as to evaluate the antimicrobial resistance conferred by these genes. We prospectively analyzed 1273 fecal specimens of farm and domestic animals for the isolation of enterobacteria that had the ESBL phenotype by using biochemical methods. The extracted genes were amplified by polymerase chain reaction and sequenced for the characterization of blaSHV, blaTEM, and blaCTX-M variants. The drug-resistance spectrum and hierarchical clusters were analyzed against 19 antibacterial agents. Out of 245 (19.2%) ESBL enterobacteria, 180 (75.5%) Escherichia coli and 34 (13.9%) Klebsiella pneumoniae were prevalent species. A total of 73.9% blaCTX-M, 26.1% blaTEM, and 14.2% blaSHV were found among the enterobacteria; however, their association with farm or domestic animals was not statistically significant. The distribution of bla gene variants showed the highest number of blaCTX-M-1 (133; 54.3%), followed by blaCTX-M-15 (28; 11.4%), blaTEM-52 (40; 16.3%), and blaSHV-12 (22; 9%). In addition, 84.5% of the enterobacteria had the integrons intI1. We observed ±100% enterobacteria resistant to cephalosporin, 7 (2.9%) to colistin (minimum inhibitory concentration breakpoint ≥4 μg/mL), 9 (3.7%) to piperacillin-tazobactam, 11 (4.5%) to imipenem, 14 (5.7%) to meropenem, and 18 (7.3%) to cefoperazone-sulbactam, without statistically significant association. Animal gut microbiota contain a considerable number of blaCTX-M, blaTEM, blaSHV, and integrons, which are a potential source of acquired extensive drug resistance in human strains and leaves fewer therapeutic substitutes.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Development of multivalent vaccine targeting M segment of Crimean Congo Hemorrhagic Fever Virus (CCHFV) using immunoinformatic approaches

Maaza Sana / Aneela Javed / Syed Babar Jamal / Muhammad Junaid / Muhammad Faheem

Saudi Journal of Biological Sciences, Vol 29, Iss 4, Pp 2372-

2022 Volume 2388

Abstract: ... in M segment) of CCHFV with an N-terminus human β-defensin as an adjuvant, as well as an N-terminus ...

Abstract	Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne viral infection with no licensed vaccine or therapeutics available for its treatment. In the present study we have developed the first multi-epitope subunit vaccine effective against all the seven genotypes of CCHF virus (CCHFV). The vaccine contains five B-cell, two MHC-II (HTL), and three MHC-I (CTL) epitopes screened from two structural glycoproteins (Gc and Gn in M segment) of CCHFV with an N-terminus human β-defensin as an adjuvant, as well as an N-terminus EAAAK sequence. The epitopes were rigorously investigated for their antigenicity, allergenicity, IFN gamma induction, anti-inflammatory responses, stability, and toxicity. The three-dimensional structure of the vaccine was predicted and docked with TLR-3, TLR-8, and TLR-9 receptors to find the strength of the binding complexes via molecular dynamics simulation. After codon adaptation, the subunit vaccine construct was developed in a pDual-GC plasmid and has population coverage of 98.47% of the world's population (HLA-I & II combined). The immune simulation studies were carried out on the C-ImmSim in-silico interface showing a marked increase in the production of cellular and humoral response (B-cell and T-cell) as well as TGFβ, IL-2, IL-10, and IL-12 indicating that the proposed vaccine would be able to sufficiently provoke both humoral and cell-mediated immune responses. Thus, making it a new and promising vaccine candidate against CCHFV.
Keywords	CCHFV ; Vaccine ; Glycoproteins ; Adjuvant ; B-cell ; T-cell ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article: Insights into the nonlinear optical (NLO) response of pure Aum (2 ≥ m ≤ 7) and copper-doped Auₘ–xCuₓ clusters

Hussain, Fakhar / Hussain, Riaz / Adnan, Muhammad / Muhammad, Shabbir / Irshad, Zobia / Khan, Muhammad Usman / Yaqoob, Junaid / Ayub, Khurshid

RSC advances. 2022 Sept. 07, v. 12, no. 39

2022

Abstract: A series of small pure Auₘ (2 ≥ m ≤ 7) and copper-doped Auₘ₋ₓCuₓ clusters was evaluated by density ...

Abstract	A series of small pure Auₘ (2 ≥ m ≤ 7) and copper-doped Auₘ₋ₓCuₓ clusters was evaluated by density functional theory (DFT) at the CAM-B3LYP/LANL2DZ level for their geometric, electronic, and nonlinear optical (NLO) properties. The charge transfer for the Au cluster significantly improved by reducing the HOMO–LUMO energy gap from 3.67 eV to 0.91 eV after doping with Cu atoms. The doping of Cu also showed noteworthy impacts on other optical and NLO properties, including a decrease in the excitation energy and increase in the dipole moment and oscillator strength. Furthermore, changes in the linear isotropic and anisotropic polarizabilities (αᵢₛₒ and αₐₙᵢₛₒ) and first and second NLO hyperpolarizabilities (βₛₜₐₜᵢc, γₛₜₐₜᵢc) were also observed in the pure and Cu-doped clusters, which enhanced the NLO response. The nonlinear optical properties of the clusters were evaluated by calculating the static and frequency dependent second- and third-order NLO polarizabilities at 1064 nm wavelength. Among all the doped structures, the Au₃Cu₁ cluster showed the largest static first hyperpolarizability of β₍ₜₒₜₐₗ₎ = 4.73 × 10³ au, while the Au₁Cu₆ cluster showed frequency dependent first hyperpolarizability of β₍₋₂w;w,w₎ = 1.26 × 10⁶ au. Besides this, large static and frequency-dependent second hyperpolarizability values of 6.30 × 10⁵ au and 1.05 × 10 au were exhibited by Cu₇ and Au₁Cu₆, respectively. This study offers an effective approach to design high-performance NLO materials utilizing mixed metal clusters which might have broad applications in the fields of optoelectronics and electronics.
Keywords	anisotropy ; density functional theory ; electronics ; energy ; geometry ; isotropy ; wavelengths
Language	English
Dates of publication	2022-0907
Size	p. 25143-25153.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/d2ra03664a
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