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  1. Article: Antibiotic resistance associated lactic acid cross tolerance in Shiga-toxin producing

    Oguadinma, Ikechukwu / Mishra, Abhinav / Dev Kumar, Govindaraj

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1059144

    Abstract: Introduction: The occurrence of antibiotic resistant (ABR) bacteria in foods is a growing public health challenge. We evaluated sanitizer cross-tolerance among ABR : Methods: Resistance to ampicillin and streptomycin were evolved in : Results: The ...

    Abstract Introduction: The occurrence of antibiotic resistant (ABR) bacteria in foods is a growing public health challenge. We evaluated sanitizer cross-tolerance among ABR
    Methods: Resistance to ampicillin and streptomycin were evolved in
    Results: The minimum inhibitory concentration (MIC) of lactic acid for all strains evaluated was 0.375% v/v. Analysis of bacterial growth parameters in tryptic soy broth amended with 0.0625% v/v, 0.125% v/v, and 0.25% v/v (subMIC) lactic acid indicated that growth correlated positively with the lag phase duration, and negatively with both the maximum growth rate and change in population density for all strains evaluated except for the highly tolerant variant- O157:H7 amp P strep C. Strains O121 NR (non-ABR), O121 amp C, O121 amp P strep C, O157:H7 H1730 amp C and O157:H7 H1730 amp P strep C were not inactivated after exposure to 1% and 2.5% v/v lactic acid for 300 s. No recovery of cells was observed after the strains were exposed to 5% v/v lactic acid for 300 s. ABR strains O157:H7 H1730 amp C and O157: H7 H1730 amp P strep C demonstrated a high tolerance to lactic acid (
    Conclusion: ABR in isolate
    Language English
    Publishing date 2023-04-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1059144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Comparison of three air sampling methods for the quantification of Salmonella, Shiga-toxigenic Escherichia coli (STEC), coliforms, and generic E. coli from bioaerosols of cattle and poultry farms.

    Ruiz-Llacsahuanga, Blanca / Sanchez-Tamayo, Martha / Dev Kumar, Govindaraj / Critzer, Faith

    Journal of food protection

    2024  , Page(s) 100282

    Abstract: Recent fresh produce outbreaks potentially associated with bioaerosol contamination from animal operations in adjacent land highlighted the need for further study to better understand the associated risk. The purpose of this research was to evaluate ... ...

    Abstract Recent fresh produce outbreaks potentially associated with bioaerosol contamination from animal operations in adjacent land highlighted the need for further study to better understand the associated risk. The purpose of this research was to evaluate three sampling methods for quantifying target bacterial bioaerosols from animal operations. A dairy cattle and poultry farm located in Georgia, U.S. were visited six times each. Air was collected for 10 min using: 2-stage Andersen impactor with and without mineral oil overlay and impingement samplers. Sampling devices were run concurrently at 0.1, 1, and 2 m heights (n=36). Andersen samplers were loaded with CHROMagar™ Salmonella, CHROMagar™ STEC, or Brilliance™ coliforms/E. coli. The impingement sampler contained buffered peptone water (20 mL) which was vacuum filtered through a 0.45 µm filter and placed onto the respective media. Plates were incubated at 37 ℃ for 48 h. PCR confirmation followed targeting ttr for Salmonella and stx
    Language English
    Publishing date 2024-04-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.1016/j.jfp.2024.100282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Assessing the Risk of Seasonal Effects of

    Xu, Xinran / Rothrock, Michael J / Dev Kumar, Govindaraj / Mishra, Abhinav

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 13

    Abstract: Campylobacter has consistently posed a food safety issue in broiler meat. This study aimed to create a quantitative microbial risk assessment model from retail to consumption, designed to evaluate the seasonal risk of campylobacteriosis associated with ... ...

    Abstract Campylobacter has consistently posed a food safety issue in broiler meat. This study aimed to create a quantitative microbial risk assessment model from retail to consumption, designed to evaluate the seasonal risk of campylobacteriosis associated with broiler meat consumption in the United States. To achieve this, data was gathered to build distributions that would enable us to predict the growth of Campylobacter during various stages such as retail storage, transit, and home storage. The model also included potential fluctuations in concentration during food preparation and potential cross-contamination scenarios. A Monte Carlo simulation with 100,000 iterations was used to estimate the risk of infection per serving and the number of infections in the United States by season. In the summer, chicken meat was estimated to have a median risk of infection per serving of 9.22 × 10
    Language English
    Publishing date 2023-06-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12132559
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  4. Article ; Online: Effect of aquaponic water and substratum material on biofilm formation by Aeromonas hydrophila.

    Dorick, Jennifer M / Macarisin, Dumitru / Dunn, Laurel / Dev Kumar, Govindaraj

    International journal of food microbiology

    2023  Volume 404, Page(s) 110316

    Abstract: Aeromonas hydrophila is a zoonotic pathogen causing illness in fish and susceptible humans. This emerging pathogen has been isolated within aquaponic systems and could cause disease in fish and a hazard to humans consuming aquaponic produce. This study ... ...

    Abstract Aeromonas hydrophila is a zoonotic pathogen causing illness in fish and susceptible humans. This emerging pathogen has been isolated within aquaponic systems and could cause disease in fish and a hazard to humans consuming aquaponic produce. This study determined whether A. hydrophila from an aquaponic farm could form biofilms in aquaponic water and on materials used in these systems. A. hydrophila biofilm biomass and cell density in aquaponic water were evaluated by crystal violet staining and culture-based enumeration. Biofilm biomass and biofilm cell density were affected by the water source and A. hydrophila isolate (P < 0.05). A. hydrophila formed the most biomass from the beginning of deep-water culture (BDWC) water (OD
    MeSH term(s) Humans ; Animals ; Aeromonas hydrophila ; Water ; Biofilms ; Fishes ; Aquaculture ; Fish Diseases
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2023-07-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 87122-9
    ISSN 1879-3460 ; 0168-1605
    ISSN (online) 1879-3460
    ISSN 0168-1605
    DOI 10.1016/j.ijfoodmicro.2023.110316
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  5. Article ; Online: Sunlight Parameters Influence the Survival and Decline of Salmonella and Escherichia coli in Water.

    Oguadinma, Ikechukwu Chukwuma / Mishra, Abhinav / Kumar, Govindaraj Dev

    Journal of food protection

    2022  Volume 85, Issue 11, Page(s) 1614–1624

    MeSH term(s) Animals ; Food Microbiology ; Colony Count, Microbial ; Water ; Sunlight ; Food Handling ; Salmonella ; Escherichia coli O157 ; Temperature ; Anti-Bacterial Agents
    Chemical Substances Water (059QF0KO0R) ; Anti-Bacterial Agents
    Language English
    Publishing date 2022-08-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/JFP-22-042
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Longitudinal Survey of Aeromonas hydrophila and Foodborne Pathogens in a Commercial Aquaponics System.

    Dorick, Jennifer / Kumar, Govindaraj Dev / Macarisin, Dumitru / Andrew Widmer, J / Stivers, Tori / Dunn, Laurel L

    Journal of food protection

    2024  Volume 87, Issue 3, Page(s) 100230

    Abstract: Aquaponic production of fresh produce is a sustainable agricultural method becoming widely adopted, though few studies have investigated potential food safety hazards within commercial systems. A longitudinal study was conducted to isolate and quantify ... ...

    Abstract Aquaponic production of fresh produce is a sustainable agricultural method becoming widely adopted, though few studies have investigated potential food safety hazards within commercial systems. A longitudinal study was conducted to isolate and quantify several foodborne pathogens from a commercial, aquaponic farm, and to elucidate their distribution throughout. The survey was conducted over 2 years on a controlled-environment farm containing Nile tilapia (Oreochromis niloticus) and lettuce (Lactuca sativa). Samples (N = 1,047) were collected bimonthly from three identical, independent systems, and included lettuce leaves, roots, fingerlings (7-126 d old), feces from mature fish (>126 d old), water, and sponge swabs collected from the tank interior surface. Most probable number of generic Escherichia coli were determined using IDEXX Colilert Quanti-Tray. Enumeration and enrichment were used to detect Shiga toxin-producing E. coli (STEC), Salmonella enterica, Listeria monocytogenes, Aeromonas spp., Aeromonas hydrophilia, and Pseudomonas aeruginosa. Generic E. coli, STEC, L. monocytogenes, and S. enterica were not detected in collected samples. P. aeruginosa was isolated from water (7/351; 1.99%), swabs (3/351; 0.85%), feces (2/108; 1.85%), and lettuce leaves (2/99; 2.02%). A. hydrophila was isolated from all sample types (623/1047; 59.50%). The incidence of A. hydrophila in water (X
    MeSH term(s) Animals ; Escherichia coli ; Aeromonas hydrophila ; Longitudinal Studies ; Agriculture/methods ; Water
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2024-01-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.1016/j.jfp.2024.100230
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Inactivation of dried cells and biofilms of

    Olszewska, Magdalena A / Dev Kumar, Govindaraj / Hur, Minji / Diez-Gonzalez, Francisco

    Applied and environmental microbiology

    2023  Volume 89, Issue 10, Page(s) e0114723

    Abstract: Antimicrobial blue light (aBL) in the 400-470 nm wavelength range has been reported to kill multiple bacteria. This study assessed its potential for mitigating an important foodborne pathogen, ...

    Abstract Antimicrobial blue light (aBL) in the 400-470 nm wavelength range has been reported to kill multiple bacteria. This study assessed its potential for mitigating an important foodborne pathogen,
    MeSH term(s) Listeria monocytogenes ; Colony Count, Microbial ; Biofilms ; Anti-Infective Agents/pharmacology ; Light ; Stainless Steel ; Food Microbiology
    Chemical Substances Anti-Infective Agents ; Stainless Steel (12597-68-1)
    Language English
    Publishing date 2023-10-17
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/aem.01147-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Antibiotic Resistance Influences Growth Rates and Cross-Tolerance to Lactic Acid in

    Oguadinma, Ikechukwu Chukwuma / Mishra, Abhinav / Juneja, Vijay K / Dev Kumar, Govindaraj

    Foodborne pathogens and disease

    2022  Volume 19, Issue 9, Page(s) 622–629

    Abstract: Escherichia ... ...

    Abstract Escherichia coli
    MeSH term(s) Ampicillin ; Animals ; Anti-Bacterial Agents/pharmacology ; Cattle ; Colony Count, Microbial ; Drug Resistance, Microbial ; Escherichia coli O157 ; Food Microbiology ; Lactic Acid/pharmacology ; Streptomycin/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Lactic Acid (33X04XA5AT) ; Ampicillin (7C782967RD) ; Streptomycin (Y45QSO73OB)
    Language English
    Publishing date 2022-07-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2148479-X
    ISSN 1556-7125 ; 1535-3141
    ISSN (online) 1556-7125
    ISSN 1535-3141
    DOI 10.1089/fpd.2022.0009
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  9. Article: Evaluation of Low-Cost Smartphone-Based Infrared Cameras to Assess the Cooling and Refrigerated Storage Temperatures of Fresh Produce.

    Yang, Boran / Dev Kumar, Govindaraj / Mis Solval, Kevin

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 21

    Abstract: Populations of pathogens may increase in fresh produce when subjected to temperature abuse. Smartphone-based infrared (SBIR) cameras are potential alternatives for temperature measurements of fresh produce during postharvest handling and storage. This ... ...

    Abstract Populations of pathogens may increase in fresh produce when subjected to temperature abuse. Smartphone-based infrared (SBIR) cameras are potential alternatives for temperature measurements of fresh produce during postharvest handling and storage. This study compared the performance of SBIR cameras (FLIR and Seek) against conventional temperature acquisition devices for evaluating fresh produce's simulated hydrocooling and storage conditions. First, thermal images of fresh produce were obtained with SBIR cameras and handheld thermal imagers at ~35 °C, ~20 °C, and ~4 °C to simulate outdoor, packinghouse, and refrigerated environments, respectively. Next, fresh produce was incubated at ~42 °C for 20 h and immersed in chilled water for a hydrocooling simulation. Then, boxes containing cooled fresh produce were stored in a walk-in cooler at different heights for three days. FLIR SBIR cameras were more effective at capturing thermal images of fresh produce than Seek SBIR cameras in all evaluated conditions. More importantly, SBIR cameras accurately acquired temperature profiles of fresh produce during simulated hydrocooling and cold storage. Additionally, the accuracy and quality of thermal images obtained with FLIR cameras were better than those obtained with Seek cameras. The study demonstrated that SBIR cameras are practical, easy-to-use, and cost-effective devices to monitor fresh produce's temperature during postharvest handling and storage.
    Language English
    Publishing date 2022-10-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11213440
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  10. Article: Transfer of generic

    Bardsley, Cameron A / Chasteen, Kaicie / Shapiro-Ilan, David / Bock, Clive H / Niemira, Brendan A / Kumar, Govindaraj Dev

    Heliyon

    2023  Volume 9, Issue 9, Page(s) e19676

    Abstract: During harvest pecan nuts are at risk of contamination with foodborne pathogens from extended contact with the ground. The objective of this study was to determine the potential transfer ... ...

    Abstract During harvest pecan nuts are at risk of contamination with foodborne pathogens from extended contact with the ground. The objective of this study was to determine the potential transfer of
    Language English
    Publishing date 2023-08-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e19676
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