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  1. Article ; Online: Biofoundries are a nucleating hub for industrial translation.

    Farzaneh, Tabasum / Freemont, Paul S

    Synthetic biology (Oxford, England)

    2021  Volume 6, Issue 1, Page(s) ysab013

    Abstract: Contemporary synthetic biology embraces the entire innovation pipeline; it is a transformative technology platform impacting new applications and improving existing industrial products and processes. However, challenges still emerge at the interface of ... ...

    Abstract Contemporary synthetic biology embraces the entire innovation pipeline; it is a transformative technology platform impacting new applications and improving existing industrial products and processes. However, challenges still emerge at the interface of upstream and downstream processes, integral to the value chain. It is now clear that biofoundries have a key role to play in addressing this; they provide unique and accessible infrastructure to drive the standardization necessary to deliver systematic design and engineering of biological systems and workflows. As for other biofoundries, the success of the London Biofoundry has been in part due to its expertise in establishing channels for industrial translation through its extensive strategic collaborations. It has also become cemented as a key component of various consortia and partnerships that serve the broader bioeconomy and industrial strategies. Adopting a networked approach enables links to be made between infrastructure, researchers, industrialists and policy makers to de-risk the economic challenges of scale-up, as well as contribute to the growing bioeconomy.
    Language English
    Publishing date 2021-06-23
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2397-7000
    ISSN (online) 2397-7000
    DOI 10.1093/synbio/ysab013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Differences in the mechanism of metabolic regulation of ATP-sensitive K+ channels containing Kir6.1 and Kir6.2 subunits.

    Farzaneh, Tabasum / Tinker, Andrew

    Cardiovascular research

    2008  Volume 79, Issue 4, Page(s) 621–631

    Abstract: Aims: ATP sensitive K(+) channels (K(ATP)) sense adenine nucleotide concentrations and thus couple the metabolic state of the cell to membrane potential. The hetero-octameric complex of a sulphonylurea receptor (SUR2B) and an inwardly rectifying K(+) ... ...

    Abstract Aims: ATP sensitive K(+) channels (K(ATP)) sense adenine nucleotide concentrations and thus couple the metabolic state of the cell to membrane potential. The hetero-octameric complex of a sulphonylurea receptor (SUR2B) and an inwardly rectifying K(+) channel (Kir6.1) and the corresponding native channel in smooth muscle are relatively insensitive to variations in intracellular ATP. Activation of these channels in blood vessels during hypoxia/ischaemia is thought to be mediated via hormonal regulation such as cellular adenosine release or the release of mediators from the endothelium. In contrast, intracellular ATP prominently inhibits Kir6.2 containing complexes, such as those present in cardiac myocytes. Thus, we investigated differences in the mechanism of metabolic regulation of Kir6.1 and Kir6.2 containing K(ATP) channels.
    Methods and results: We have heterologously expressed K(ATP) channel subunits in HEK293 and CHO cells and studied their function using (86)Rb efflux and patch clamping. We show that rodent Kir6.1/SUR2B has direct intrinsic metabolic sensitivity independent of any regulation by protein kinase A. In contrast to Kir6.2 containing complexes, this was not endowed by the ATP sensitivity of the pore forming subunit but was instead a property of the SUR2B subunit. Mutagenesis of key residues within the nucleotide-binding domains (NBD) implicated both domains in governing the metabolic sensitivity.
    Conclusion: Kir6.1\SUR2B has intrinsic sensitivity to metabolism endowed by the likely processing of adenine nucleotides at the NBD of SUR2B.
    MeSH term(s) ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Adenine Nucleotides/metabolism ; Animals ; Binding Sites ; CHO Cells ; Cricetinae ; Cricetulus ; Energy Metabolism ; Humans ; KATP Channels/genetics ; KATP Channels/metabolism ; Membrane Potentials ; Patch-Clamp Techniques ; Point Mutation ; Potassium Channels, Inwardly Rectifying/genetics ; Potassium Channels, Inwardly Rectifying/metabolism ; Protein Binding ; Protein Structure, Tertiary ; Receptors, Drug/genetics ; Receptors, Drug/metabolism ; Rubidium Radioisotopes ; Sulfonylurea Receptors ; Time Factors ; Transfection
    Chemical Substances Adenine Nucleotides ; KATP Channels ; Kir6.2 channel ; Potassium Channels, Inwardly Rectifying ; Receptors, Drug ; Rubidium Radioisotopes ; Sulfonylurea Receptors ; uK-ATP-1 potassium channel
    Language English
    Publishing date 2008-09-01
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80340-6
    ISSN 1755-3245 ; 0008-6363
    ISSN (online) 1755-3245
    ISSN 0008-6363
    DOI 10.1093/cvr/cvn138
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 565: Show issues Location:
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  3. Article: Complex ABCC8 DNA variations in congenital hyperinsulinism: lessons from functional studies.

    Muzyamba, Morris / Farzaneh, Tabasum / Behe, Phillip / Thomas, Alison / Christesen, Henrik B T / Brusgaard, Klaus / Hussain, Khalid / Tinker, Andrew

    Clinical endocrinology

    2007  Volume 67, Issue 1, Page(s) 115–124

    Abstract: Objective: Congenital hyperinsulinism (CHI) is a cause of persistent and severe hypoglycaemia in infancy. Mutations in the genes ABCC8 and KCNJ11 encoding SUR1 and Kir6.2, respectively, are the commonest cause of CHI. We investigated whether the ... ...

    Abstract Objective: Congenital hyperinsulinism (CHI) is a cause of persistent and severe hypoglycaemia in infancy. Mutations in the genes ABCC8 and KCNJ11 encoding SUR1 and Kir6.2, respectively, are the commonest cause of CHI. We investigated whether the possession of two DNA variants leading to coding changes in a single allele of ABCC8 can affect the potential mechanism of disease pathogenesis.
    Design and patients: We studied two patients with complex mutations in the ABCC8 gene with CHI and used in vitro studies to explore the potential disease mechanism and the contribution of the various mutant allelles.
    Results: The first case had diffuse disease and was homozygous for the mutations D1193V and R1436Q in SUR1. Channel complexes containing the D1193V mutant were delivered to the plasma membrane and were functional and those containing R1436Q were also present at the plasma membrane but were nonfunctional. Combining the two mutations (SUR1D1193V/R1436Q) led to intracellular retention of the channel complex. In a second family, the patient had histologically focal disease and was heterozygous for two mutations from his father (G228D and D1471N) and one from his mother (V1572I). SUR1 G228D and D1471N singly or in combination led to intracellular retention of the channel complex and loss of function. By contrast, V1572I is trafficked appropriately and is functional, consistent with a mechanism of reduction to hemizygosity of paternal ABCC8 in focal disease. V1572I is likely to be a benign DNA variant.
    Conclusion: In one patient the combination of two coding variants led to intracellular retention of channel complex. In a second patient, functional studies allowed us to unravel the DNA variants likely to be causing the abrogation of ATP-sensitive K(+) channel function.
    MeSH term(s) ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Adult ; Animals ; Blotting, Western ; CHO Cells ; Case-Control Studies ; Congenital Hyperinsulinism/genetics ; Cricetinae ; Cricetulus ; Gene Expression ; Genotype ; Humans ; Infant, Newborn ; Male ; Mice ; Mutagenesis, Site-Directed ; Mutation ; Phenotype ; Potassium Channels/genetics ; Potassium Channels/metabolism ; Potassium Channels, Inwardly Rectifying/genetics ; Potassium Channels, Inwardly Rectifying/metabolism ; Receptors, Drug/genetics ; Receptors, Drug/metabolism ; Staining and Labeling ; Sulfonylurea Receptors ; Transfection/methods
    Chemical Substances ABCC8 protein, human ; Abcc8 protein, mouse ; Kir6.2 channel ; Potassium Channels ; Potassium Channels, Inwardly Rectifying ; Receptors, Drug ; Sulfonylurea Receptors
    Language English
    Publishing date 2007-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 121745-8
    ISSN 1365-2265 ; 0300-0664
    ISSN (online) 1365-2265
    ISSN 0300-0664
    DOI 10.1111/j.1365-2265.2007.02847.x
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 814: Show issues Location:
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  4. Article: Metacaspase-8 modulates programmed cell death induced by ultraviolet light and H2O2 in Arabidopsis.

    He, Rui / Drury, Georgina E / Rotari, Vitalie I / Gordon, Anna / Willer, Martin / Farzaneh, Tabasum / Woltering, Ernst J / Gallois, Patrick

    The Journal of biological chemistry

    2007  Volume 283, Issue 2, Page(s) 774–783

    Abstract: Programmed cell death (PCD) is a genetically controlled cell death that is regulated during development and activated in response to environmental stresses or pathogen infection. The degree of conservation of PCD across kingdoms and phylum is not yet ... ...

    Abstract Programmed cell death (PCD) is a genetically controlled cell death that is regulated during development and activated in response to environmental stresses or pathogen infection. The degree of conservation of PCD across kingdoms and phylum is not yet clear; however, whereas caspases are proteases that act as key components of animal apoptosis, plants have no orthologous caspase sequences in their genomes. The discovery of plant and fungi metacaspases as proteases most closely related to animal caspases led to the hypothesis that metacaspases are the functional homologues of animal caspases in these organisms. Arabidopsis thaliana has nine metacaspase genes, and so far it is unknown which members of the family if any are involved in the regulation of PCD. We show here that metacaspase-8 (AtMC8) is a member of the gene family strongly up-regulated by oxidative stresses caused by UVC, H(2)O(2), or methyl viologen. This up-regulation was dependent of RCD1, a mediator of the oxidative stress response. Recombinant metacaspase-8 cleaved after arginine, had a pH optimum of 8, and complemented the H(2)O(2) no-death phenotype of a yeast metacaspase knock-out. Overexpressing AtMC8 up-regulated PCD induced by UVC or H(2)O(2), and knocking out AtMC8 reduced cell death triggered by UVC and H(2)O(2) in protoplasts. Knock-out seeds and seedlings had an increased tolerance to the herbicide methyl viologen. We suggest that metacaspase-8 is part of an evolutionary conserved PCD pathway activated by oxidative stress.
    MeSH term(s) Apoptosis/drug effects ; Apoptosis/physiology ; Apoptosis/radiation effects ; Arabidopsis ; Arabidopsis Proteins/drug effects ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/radiation effects ; Caspase 8/drug effects ; Caspase 8/genetics ; Caspase 8/radiation effects ; Cell Death ; Cysteine Endopeptidases/deficiency ; Cysteine Endopeptidases/drug effects ; Cysteine Endopeptidases/genetics ; Cysteine Endopeptidases/radiation effects ; DNA Primers ; Gene Expression Regulation, Enzymologic/drug effects ; Gene Expression Regulation, Enzymologic/radiation effects ; Gene Expression Regulation, Plant/drug effects ; Gene Expression Regulation, Plant/radiation effects ; Hydrogen Peroxide/pharmacology ; Oxidative Stress ; Plants, Genetically Modified/metabolism ; Protoplasts/drug effects ; Protoplasts/physiology ; Protoplasts/radiation effects ; Reverse Transcriptase Polymerase Chain Reaction ; Ultraviolet Rays
    Chemical Substances Arabidopsis Proteins ; DNA Primers ; Hydrogen Peroxide (BBX060AN9V) ; Caspase 8 (EC 3.4.22.-) ; Cysteine Endopeptidases (EC 3.4.22.-) ; metacaspase-8, Arabidopsis (EC 3.4.22.-)
    Language English
    Publishing date 2007-11-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.M704185200
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    Uc I Zs.108: Show issues Location:
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  5. Article: Metacaspase-8 Modulates Programmed Cell Death Induced by Ultraviolet Light and H₂O₂ in Arabidopsis

    He, Rui / Drury, Georgina E / Rotari, Vitalie I / Gordon, Anna / Willer, Martin / Farzaneh, Tabasum / Woltering, Ernst J / Gallois, Patrick

    Journal of biological chemistry. 2008 Jan. 11, v. 283, no. 2

    2008  

    Abstract: Programmed cell death (PCD) is a genetically controlled cell death that is regulated during development and activated in response to environmental stresses or pathogen infection. The degree of conservation of PCD across kingdoms and phylum is not yet ... ...

    Abstract Programmed cell death (PCD) is a genetically controlled cell death that is regulated during development and activated in response to environmental stresses or pathogen infection. The degree of conservation of PCD across kingdoms and phylum is not yet clear; however, whereas caspases are proteases that act as key components of animal apoptosis, plants have no orthologous caspase sequences in their genomes. The discovery of plant and fungi metacaspases as proteases most closely related to animal caspases led to the hypothesis that metacaspases are the functional homologues of animal caspases in these organisms. Arabidopsis thaliana has nine metacaspase genes, and so far it is unknown which members of the family if any are involved in the regulation of PCD. We show here that metacaspase-8 (AtMC8) is a member of the gene family strongly up-regulated by oxidative stresses caused by UVC, H₂O₂, or methyl viologen. This up-regulation was dependent of RCD1, a mediator of the oxidative stress response. Recombinant metacaspase-8 cleaved after arginine, had a pH optimum of 8, and complemented the H₂O₂ no-death phenotype of a yeast metacaspase knock-out. Overexpressing AtMC8 up-regulated PCD induced by UVC or H₂O₂, and knocking out AtMC8 reduced cell death triggered by UVC and H₂O₂ in protoplasts. Knock-out seeds and seedlings had an increased tolerance to the herbicide methyl viologen. We suggest that metacaspase-8 is part of an evolutionary conserved PCD pathway activated by oxidative stress.
    Language English
    Dates of publication 2008-0111
    Size p. 774-783.
    Publishing place American Society for Biochemistry and Molecular Biology
    Document type Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    Database NAL-Catalogue (AGRICOLA)

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