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Artikel ; Online: IL-25 Impact on Malignant B Cells Survival and T Cells Activation in Chronic Lymphocytic Leukemia.

Pashei, Mehrnoosh / Ghahremanfard, Farahnaz / Manouchehri Doulabi, Ehsan / Hemmati, Maral / Pak, Fatemeh / Kokhaei, Parviz

Iranian journal of allergy, asthma, and immunology

2023 Band 22, Heft 3, Seite(n) 299–311

Abstract: T cell dysregulation and shift to T helper 2 responses, boosting tumor microenvironment support, contributes to the survival of leukemic B cells in Chronic Lymphocytic Leukemia. Interleukin (IL)-25 is involved in the initiation of T helper 2 cell ... ...

Abstract	T cell dysregulation and shift to T helper 2 responses, boosting tumor microenvironment support, contributes to the survival of leukemic B cells in Chronic Lymphocytic Leukemia. Interleukin (IL)-25 is involved in the initiation of T helper 2 cell responses. Signal transduction of IL-25 begins with the heterodimer receptor (IL-17RA/IL-17RB). The presence of IL-25 in the tumor microenvironment may affect the supportive effects of T cells in the surrounding tumor cell environment. The purpose of this study was to evaluate the role of IL-25 in the biology of CLL. IL-17RB expression in CD3+ and CD19+ cells was assessed in isolated peripheral blood mononuclear cells (PBMCs) of nine CLL patients and nine healthy subjects by real-time polymerase chain reaction and flow cytometry. B cells were positively enriched from PBMCs using magnetic-activated cell sorting (MACS). PBMCs and purified leukemic B cells were cultured with recombinant human IL-25 (20ng/ml) for 72 hours, then the viability and apoptosis of cultured cells were measured by MTT assay and AnnexinV/7AAD. Furthermore, the levels of CD69 expression on T lymphocytes and IL-17RB in T and B cells were determined by flow cytometry. The basal level of IL-17RB expression in CLL patients was significantly higher than that in control individuals. In addition, the percentage of IL-17RB+/CD3+, IL-17RB+/CD19+ cells and CD69+/CD3+ cells increased after 72 hours of culture with IL-25 in CLL patients compared to healthy subjects. IL-25 also reduces the apoptosis rate of tumor cells. We found that IL-25 could stimulate T cells in CLL patients and lower B cell death. This suggests that IL-25 might have a role in enhancing the survival of tumor cell by expressing receptors for inflammation, such as IL-17RB, and might be involved in the development of CLL.
Mesh-Begriff(e)	Humans ; B-Lymphocytes ; Cells, Cultured ; Leukemia, Lymphocytic, Chronic, B-Cell ; Leukocytes, Mononuclear/metabolism ; Lymphocyte Activation ; Tumor Microenvironment
Chemische Substanzen	IL25 protein, human
Sprache	Englisch
Erscheinungsdatum	2023-06-16
Erscheinungsland	Iran
Dokumenttyp	Journal Article
ZDB-ID	2488724-9
ISSN	1735-5249 ; 1735-1502
ISSN (online)	1735-5249
ISSN	1735-1502
DOI	10.18502/ijaai.v22i3.13058
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 6806: Hefte anzeigen

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Artikel ; Online: Enhancement of targeted therapy in combination with metformin on human breast cancer cell lines.

Mahmoudi, Ghazal / Ehteshaminia, Yahya / Kokhaei, Parviz / Jalali, Seyedeh Farzaneh / Jadidi-Niaragh, Farhad / Pagheh, Abdol Sattar / Enderami, Seyed Ehsan / Kenari, Saeid Abedian / Hassannia, Hadi

Cell communication and signaling : CCS

2024 Band 22, Heft 1, Seite(n) 10

Abstract: Background: Breast cancer remains a primary global health concern due to its limited treatment options, frequent disease recurrence, and high rates of morbidity and mortality. Thereby, there is a need for more effective treatment approaches. The ... ...

Abstract	Background: Breast cancer remains a primary global health concern due to its limited treatment options, frequent disease recurrence, and high rates of morbidity and mortality. Thereby, there is a need for more effective treatment approaches. The proposal suggests that the combination of targeted therapy with other antitumoral agents could potentially address drug resistance. In this study, we examined the antitumoral effect of combining metformin, an antidiabetic drug, with targeted therapies, including tamoxifen for estrogen receptor-positive (MCF-7), trastuzumab for HER2-positive (SKBR-3), and antibody against ROR1 receptor for triple-negative breast cancer (MDA-MB-231). Methods: Once the expression of relevant receptors on each cell line was confirmed and appropriate drug concentrations were selected through cytotoxicity assays, the antitumor effects of both monotherapy and combination therapy on colony formation, migration, invasion were assessed in in vitro as well as tumor area and metastatic potential in ex ovo Chick chorioallantoic membrane (CAM) models. Results: The results exhibited the enhanced effects of tamoxifen when combined with targeted therapy. This combination effectively inhibited cell growth, colony formation, migration, and invasion in vitro. Additionally, it significantly reduced tumor size and metastatic potential in an ex ovo CAM model. Conclusions: The findings indicate that a favorable strategy to enhance the efficacy of breast cancer treatment would be to combine metformin with targeted therapies.
Mesh-Begriff(e)	Humans ; Female ; Breast Neoplasms/pathology ; Metformin/pharmacology ; Cell Line, Tumor ; Neoplasm Recurrence, Local ; Tamoxifen/pharmacology ; Triple Negative Breast Neoplasms/pathology ; Cell Proliferation
Chemische Substanzen	Metformin (9100L32L2N) ; Tamoxifen (094ZI81Y45)
Sprache	Englisch
Erscheinungsdatum	2024-01-02
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2126315-2
ISSN	1478-811X ; 1478-811X
ISSN (online)	1478-811X
ISSN	1478-811X
DOI	10.1186/s12964-023-01446-0
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Prevalence of COVID-19 Virus Infection in Semnan province.

Jandaghi, Elahe / Hemati, Maral / Mohammadlou, Maryam / Jandaghi, Jafar / Mirmohammadkhani, Majid / Danaei, Navid / Kokhaei, Parviz

Iranian journal of immunology : IJI

2021 Band 18, Heft 1, Seite(n) 74–81

Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) causing a human pandemic disease named COVID-19 has become a major global health concern. Iran as one of the most affected countries needs unprecedented effort for monitoring and ... ...

Abstract	Background: Severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) causing a human pandemic disease named COVID-19 has become a major global health concern. Iran as one of the most affected countries needs unprecedented effort for monitoring and evaluation of COVID-19. Objective: To determine the seroprevalance of COVID-19 in Semnan province North-East of Iran. Methods: Six hundred people were randomly selected using the "SIB data-base". From 1 to 30 June, 2020, 153 participants of Semnan population were enrolled. Blood, nasopharyngeal and oropharyngeal samples were obtained. Prevalence of IgM and IgG antibodies were ascertained using ELISA and Real-Time PCR was conducted to evaluate viral load. Estimates of prevalence were standardized by age and sex, based on the 2015 national census of Semnan province. Results: Seroprevalence showed no difference between females and males and no significant association between age and seropositivity. Among total participants, the age and sex adjusted prevalence of SARS-CoV2 infection was 19.3% (95% CI, 14.0-26.7 per 100 persons). Approximately 10% of participants had detectable antibodies but showed a negative-PCR result. However, approximately 80% of participants did not show an evidence of infection. Conclusion: The majority of the population in Semnan province has no detectable antibodies to SARS-CoV-2. Therefore, Semnan is considered a SARS-CoV-2 susceptible area. These results emphasize the need for maintaining public health measures to tackle the new epidemic wave.
Mesh-Begriff(e)	Adolescent ; Adult ; Age Distribution ; Aged ; Aged, 80 and over ; Antibodies, Viral/blood ; Biomarkers/blood ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/genetics ; COVID-19/immunology ; COVID-19 Nucleic Acid Testing ; COVID-19 Serological Testing ; Child ; Cross-Sectional Studies ; Female ; Host-Pathogen Interactions ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Iran/epidemiology ; Male ; Middle Aged ; Prevalence ; RNA, Viral/blood ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Seroepidemiologic Studies ; Sex Distribution ; Viral Load ; Young Adult
Chemische Substanzen	Antibodies, Viral ; Biomarkers ; Immunoglobulin G ; Immunoglobulin M ; RNA, Viral
Sprache	Englisch
Erscheinungsdatum	2021-05-04
Erscheinungsland	Iran
Dokumenttyp	Journal Article
ISSN	1735-367X
ISSN (online)	1735-367X
DOI	10.22034/iji.2021.87670.1826
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Simultaneous disruption of circulating miR-21 and cytotoxic T lymphocytes (CTLs): Prospective diagnostic and prognostic markers for esophageal squamous cell carcinoma (ESCC).

Samiei, Hadiseh / Ajam, Faezeh / Gharavi, Abdolsamad / Abdolmaleki, Sara / Kokhaei, Parviz / Mohammadi, Saeed / Memarian, Ali

Journal of clinical laboratory analysis

2021 Band 36, Heft 1, Seite(n) e24125

Abstract: Background: Esophageal squamous cell carcinoma (ESCC) as the most prominent type of esophageal cancer (EC) in developing countries encompasses a substantial contribution of cancer-related mortalities and morbidities. Cytotoxic T lymphocytes (CTLs) are ... ...

Abstract	Background: Esophageal squamous cell carcinoma (ESCC) as the most prominent type of esophageal cancer (EC) in developing countries encompasses a substantial contribution of cancer-related mortalities and morbidities. Cytotoxic T lymphocytes (CTLs) are the major subset of effector T cells against cancer. However, the microRNAs involved in the development and regulation of CTLs could be disrupted in cancers such as EC. Methods: Here, we evaluated the population of IL-10, TGF-β, IFN-γ, and IL-17a-producing CD3+CD8+ T cells, their association with the circulating levels of miR-21 and miR-29b, and their diagnostic and/or prognostic (after 160 weeks of follow-up) utilities in 34 ESCC patients (12 newly diagnosed: ND, 24 under-treatment: UT) and 34 matched healthy donors. Results: The population of IL-10 and TGF-β-producing CTLs (CD8+ Tregs) were considerably expanded, in addition to the overexpression of miR-21 in both groups (ND and UT) of ESCC patients, while the frequency of Tc17 and CD8+ Treg cells increased only in UT patients. The expression means of TGF-β and IL-10 in CTLs were considered to be excellent biomarkers (1 ≥ area under the curve: AUC ≥0.9) in distinguishing ESCC patients and associated subgroups from healthy subjects. Moreover, the lower expressions of TGF-β, IL-17a, IL-10, and IFN-γ in CTLs were associated with ESCC better prognosis. Conclusions: The association between the impaired function of CD3+ CD8+ T cell subsets and miR-21 expression could be introduced as novel therapeutic targets and powerful diagnostic and prognostic markers for ESCC.
Mesh-Begriff(e)	Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Cytokines/blood ; Esophageal Neoplasms/diagnosis ; Esophageal Neoplasms/genetics ; Esophageal Neoplasms/metabolism ; Esophageal Squamous Cell Carcinoma/diagnosis ; Esophageal Squamous Cell Carcinoma/genetics ; Esophageal Squamous Cell Carcinoma/metabolism ; Humans ; MicroRNAs/blood ; Prognosis ; T-Lymphocytes, Cytotoxic/metabolism
Chemische Substanzen	Biomarkers, Tumor ; Cytokines ; MIRN21 microRNA, human ; MicroRNAs
Sprache	Englisch
Erscheinungsdatum	2021-11-19
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	645095-7
ISSN	1098-2825 ; 0887-8013
ISSN (online)	1098-2825
ISSN	0887-8013
DOI	10.1002/jcla.24125
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: The role of the host microbiome in autism and neurodegenerative disorders and effect of epigenetic procedures in the brain functions.

Yousefi, Bahman / Kokhaei, Parviz / Mehranfar, Fatemeh / Bahar, Aisa / Abdolshahi, Anna / Emadi, Alireza / Eslami, Majid

Neuroscience and biobehavioral reviews

2021 Band 132, Seite(n) 998–1009

Abstract: Autism Spectrum Disorder (ASD) is a severe neurological/neurodegenerative syndrome that results in cognitive and communication disorders. The degree of dysbiosis is related to the severity of ASD signs. The gut is conferred with a variety of sensory ... ...

Abstract	Autism Spectrum Disorder (ASD) is a severe neurological/neurodegenerative syndrome that results in cognitive and communication disorders. The degree of dysbiosis is related to the severity of ASD signs. The gut is conferred with a variety of sensory receptors that cooperate with effector systems including the endocrine, nervous and gut immune systems of the intestine. Gut dysbiosis causes amplified inflammation, the launch of the HPA axis, changed levels of neurotransmitters and bacterial metabolites; these may donate to abnormal signaling throughout the Vagus nerve in ASD. Decreased integrity of the gastrointestinal barrier led to extreme leakage of substances as of the intestine in early life and inflammation followed by disruption of BBB integrity maybe increase the risk of ASD. Microbiota, by controlling the barrier permeability, regulate the quantity and types of bioactive materials that are transferred from the intestine to the brain. Exposure to metabolites and microbial products regulate significant procedures in the CNS, including glial cell role, myelination, synaptic pruning, and play a role in neurobehavioral, neurodegenerative, psychiatric, and metabolic syndrome.
Mesh-Begriff(e)	Autism Spectrum Disorder/metabolism ; Autistic Disorder/metabolism ; Brain/metabolism ; Epigenesis, Genetic ; Gastrointestinal Microbiome/physiology ; Humans ; Hypothalamo-Hypophyseal System/metabolism ; Microbiota ; Neurodegenerative Diseases/metabolism ; Pituitary-Adrenal System/metabolism
Sprache	Englisch
Erscheinungsdatum	2021-11-03
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Review
ZDB-ID	282464-4
ISSN	1873-7528 ; 0149-7634
ISSN (online)	1873-7528
ISSN	0149-7634
DOI	10.1016/j.neubiorev.2021.10.046
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Multistrain Probiotics Supplement Alleviates Asthma Symptoms via Increasing Treg Cells Population: A Randomized, Double-Blind, Placebo-Controlled Trial.

Abbasi-Dokht, Tannaz / Sadrifar, Sina / Forouzandeh, Sarvenaz / Malek, Farhad / Hemmati, Maral / Kokhaei, Parviz / Salek Farrokhi, Amir / Baharlou, Rasoul

International archives of allergy and immunology

2022 Band 184, Heft 3, Seite(n) 291–301

Abstract: Introduction: The favorable effects of probiotics have been demonstrated in allergic disorders. However, the underlying immunological mechanisms are poorly understood. In the present study, we investigated the improvement of clinical symptoms and ... ...

Abstract	Introduction: The favorable effects of probiotics have been demonstrated in allergic disorders. However, the underlying immunological mechanisms are poorly understood. In the present study, we investigated the improvement of clinical symptoms and immunological balance after receiving probiotics in patients with asthma. Methods: The present study was a randomized, double-blind, placebo-controlled trial in which 40 patients with asthma were enrolled. They were treated with probiotics or placebo: 1 capsule/day for 8 weeks. Pulmonary function test, percentage of CD4+ CD25+ FoxP3+ Tregs, and gene expression of T-bet, GATA-3, RORγt, and Foxp3 in PBMCs were assessed at baseline and after treatment. Results: Our results showed a significant increase in the expression of FoxP3 and CD4+ CD25+ FoxP3+ Tregs population, while RORγt and GATA3 expression were reduced. In addition, pulmonary function tests showed a significant improvement in forced expiratory volume and forced vital capacity after receiving probiotics. Discussion/conclusion: Our findings demonstrate that 8-week treatment with probiotic supplementation can control T-helper 2-predominant and Th17 pro-inflammatory responses and improve forced vital and forced expiratory volume in asthmatic patients. It seems probiotics can be used besides common treatments for patients with asthma.
Mesh-Begriff(e)	Humans ; T-Lymphocytes, Regulatory ; Nuclear Receptor Subfamily 1, Group F, Member 3/genetics ; Asthma ; Dietary Supplements ; Probiotics/therapeutic use ; Forkhead Transcription Factors/genetics
Chemische Substanzen	Nuclear Receptor Subfamily 1, Group F, Member 3 ; Forkhead Transcription Factors
Sprache	Englisch
Erscheinungsdatum	2022-12-09
Erscheinungsland	Switzerland
Dokumenttyp	Randomized Controlled Trial ; Journal Article
ZDB-ID	1108932-5
ISSN	1423-0097 ; 1018-2438
ISSN (online)	1423-0097
ISSN	1018-2438
DOI	10.1159/000526739
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Anti-tumor effect of berberine on chronic lymphocytic leukemia cells.

Abdollahi, Maryam / Mohammadlou, Maryam / Hemati, Maral / Baharlou, Rasoul / Manouchehri Doulabi, Ehsan / Ghahremanfard, Farahnaz / Sarabi, Mohammad Amir / Kokhaei, Parviz

Medical oncology (Northwood, London, England)

2022 Band 39, Heft 12, Seite(n) 217

Abstract: Chronic lymphocytic leukemia (CLL) is a blood malignancy that is characterized by remarkable expression of CD69 and Ki67 in CLL cells. Elevated levels of Cleaved-Poly (ADP-ribose) polymerase-1 (PARP1) and microRNA-155 (MiR-155) are related to poor ... ...

Abstract	Chronic lymphocytic leukemia (CLL) is a blood malignancy that is characterized by remarkable expression of CD69 and Ki67 in CLL cells. Elevated levels of Cleaved-Poly (ADP-ribose) polymerase-1 (PARP1) and microRNA-155 (MiR-155) are related to poor prognosis of disease. Berberine as a natural isoquinoline alkaloid, has shown an anti-tumor potential in tumor cells. The objective of present study was to explore some aspects of molecular mechanisms of berberine effect in CLL cells. To analyze the expression of CD69 and Ki67 using flow cytometry, 16 peripheral blood samples and seven bone marrow aspirates were collected from CLL patients. Isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) were treated with 25 µM of berberine for 24 h. The level of miR-155 expression was subsequently evaluated by real-time PCR. Furthermore, western blot was used for assessment of cleaved PARP1. Our results demonstrated a significant reduction in CD69 and Ki67 expression on CD19
Mesh-Begriff(e)	Berberine/pharmacology ; Humans ; Isoquinolines ; Ki-67 Antigen ; Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy ; Leukocytes, Mononuclear ; MicroRNAs/genetics ; Poly (ADP-Ribose) Polymerase-1
Chemische Substanzen	Isoquinolines ; Ki-67 Antigen ; MIRN155 microRNA, human ; MicroRNAs ; Berberine (0I8Y3P32UF) ; Poly (ADP-Ribose) Polymerase-1 (EC 2.4.2.30)
Sprache	Englisch
Erscheinungsdatum	2022-09-29
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	1201189-7
ISSN	1559-131X ; 0736-0118 ; 1357-0560
ISSN (online)	1559-131X
ISSN	0736-0118 ; 1357-0560
DOI	10.1007/s12032-022-01818-5
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Preparation and Characterization of PLGA Nanoparticles Containing Plasmid DNA Encoding Human IFN-lambda-1/IL-29.

Amir Kalvanagh, Parisa / Ebtekara, Masoumeh / Kokhaei, Parviz / Soleimanjahi, Hoorieh

Iranian journal of pharmaceutical research : IJPR

2019 Band 18, Heft 1, Seite(n) 156–167

Abstract: During the 15 years since the discovery of type III human interferons [IFN-λ1(IL-29), IFN-λ2(IL-28A), and IFN-λ3(IL-28B)], numerous biological properties such as anticancer, antiviral, and immunomodulatory activities of this new IFN family have been ... ...

Abstract	During the 15 years since the discovery of type III human interferons [IFN-λ1(IL-29), IFN-λ2(IL-28A), and IFN-λ3(IL-28B)], numerous biological properties such as anticancer, antiviral, and immunomodulatory activities of this new IFN family have been investigated. Several studies have shown that the encapsulation of pcDNA with PLGA nanoparticles (NPs) protects them against DNase enzyme action and increases the efficiency of gene delivery to the cells. The purpose of this study was to encapsulate pcDNA encoding IFN-λ1 (pIFN-λ1) with a simple and cost-effective method using PLGA NPs. The pIFN-λ1-loaded PLGA NPs were produced by a double-emulsion-solvent evaporation method and characterized in terms of size, size distribution, and zeta potential by DLS and morphologically by SEM and TEM. The bioactivity of NPs was also examined by fluorescent microscopy. The results showed that IFN-λ1 expressed by HEK293T cells could protect HepC-2 cells from the cytopathic effects of EMCV. The NPs were spherical in shape with a mean diameter of 380 ± 3 nm, a zeta potential of -3.3 ± 7.6 mV, an encapsulation efficiency of 75 ± 5%, and a loading capacity of 0.83 ± 0.06. The NPs were also bioactive and easily engulfed by RAW264.7 cells. The pIFN-λ1 could be sustainably released from NPs. Due to the facility and affordability of encapsulation of pIFN-λ1 in the PLGA NPs proposed in this study and the advantages of encapsulation by PLGA, it appeared rational to use pIFN-λ1-loaded NPs instead of naked pIFN-λ1 to determine other unexplained activities of this new cytokine or to use it as an alternative or adjunct to current IFN-α therapy.
Sprache	Englisch
Erscheinungsdatum	2019-04-08
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article
ZDB-ID	2578271-X
ISSN	1735-0328 ; 1726-6890
ISSN	1735-0328 ; 1726-6890
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Current information on the association of Helicobacter pylori with autophagy and gastric cancer.

Eslami, Majid / Yousefi, Bahman / Kokhaei, Parviz / Arabkari, Vahid / Ghasemian, Abdolmajid

Journal of cellular physiology

2019 Band 234, Heft 9, Seite(n) 14800–14811

Abstract: Helicobacter pylori (H. pylori) is a Gram-negative bacterium and causative agent of gastric cancer. H. pylori induce defective autophagy or inhibit it by means of CagA and vacuolating cytotoxin A (VacA) toxins leading to the gastric cancer induction. ... ...

Abstract	Helicobacter pylori (H. pylori) is a Gram-negative bacterium and causative agent of gastric cancer. H. pylori induce defective autophagy or inhibit it by means of CagA and vacuolating cytotoxin A (VacA) toxins leading to the gastric cancer induction. Impaired or defective autophagy leads to the accumulation of cytotoxic materials, such as ROS and P62 that lead to increased mutations in the DNA, genome instability, and risk of cancer formation. H. pylori CagA may inhibit autophagy through the c-Met-PI3k/Akt-mTOR signaling pathway. However, VacA induces autophagy by some signaling pathways. In the gastric epithelial cells, VacA is a necessary and sufficient factor for the creation of autophagy. While CagA is a negative regulator of this phenomenon, the elimination of this gene from H. pylori has increased autophagy and the production of inflammatory cytokines is reduced. In gastrointestinal cancers, some of the microRNAs (miRNAs) act as tumor suppressors and some other are oncogenes by regulating various genes expression. H. pylori can also modify autophagy through a mechanism that includes the function of miRNAs. In autophagy, oncogenic miRNAs inhibit activation of some tumor suppressor signaling pathways (e.g., ULK1 complex, Beclin-1 function, and Atg4 messaging), whereas tumor suppressor miRNAs can block the activation of oncogenic signaling pathways. For instance, Beclin-1 is negatively regulated by miRNA-376b (oncogenic miRNA) and miRNA-30a (tumor suppressor miRNA). Similarly, Atg4 by miRNA-376b (oncogenic miRNA) and miRNA-101 (tumor suppressor miRNA). So, this apparent paradox can be explained as that both Beclin-1 and Atg4 play different roles in a particular cell or tissue.
Sprache	Englisch
Erscheinungsdatum	2019-02-19
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Review
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.28279
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Uc I Zs.212: Hefte anzeigen

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Artikel ; Online: The Cooperation of IL-29 and PLGA Nanoparticles Improves the Protective Immunity of the gD-1 DNA Vaccine Against Herpes Simplex Virus Type 1 in Mice.

Amir Kalvanagh, Parisa / Karimi, Hesam / Soleimanjahi, Hoorieh / Ebtekar, Massoumeh / Kokhaei, Parviz / Matloubi, Zahra / Rahimi, Roghieh / Kazemi-Sefat, Nazanin Atieh / Rajaei, Hajar

Immunological investigations

2023 Band 52, Heft 7, Seite(n) 779–795

Abstract: In clinical practice, the low immunogenicity and low stability of the DNA plasmid vaccine candidates are two significant shortcomings in their application against infectious diseases. To overcome these two disadvantages, the plasmid expressing IL-29 (pIL- ...

Abstract	In clinical practice, the low immunogenicity and low stability of the DNA plasmid vaccine candidates are two significant shortcomings in their application against infectious diseases. To overcome these two disadvantages, the plasmid expressing IL-29 (pIL-29) as a genetic adjuvant and polylactic-co-glycolic acid (PLGA) as a non-viral delivery system were used, respectively. In this study, the pIL-29 encapsulated in PLGA nanoparticles (nanoIL-29) and the pgD1 encapsulated in PLGA nanoparticles (nanoVac) were simultaneously applied to boost immunologic responses against HSV-1. We generated spherical nanoparticles with encapsulation efficiency of 75 ± 5% and sustained the release of plasmids from them. Then, Balb/c mice were subcutaneously immunized twice with nanoVac+nanoIL-29, Vac+IL-29, nanoVac, Vac, nanoIL-29, and/or IL-29 in addition to negative and positive control groups. Cellular immunity was evaluated via lymphocyte proliferation assay, cytotoxicity test, and IFN-γ, IL-4, and IL-2 measurements. Mice were also challenged with 50X LD50 of HSV-1. The nanoVac+nanoIL-29 candidate vaccine efficiently enhances CTL and Th1-immune responses and increases the survival rates by 100% in mice vaccinated by co-administration of nanoVac and nanoIL-29 against the HSV-1 challenge. The newly proposed vaccine is worth studying in further clinical trials, because it could effectively improve cellular immune responses and protected mice against HSV-1.
Mesh-Begriff(e)	Animals ; Mice ; Herpesvirus 1, Human ; Glycols ; Vaccines, DNA ; Cytokines ; Mice, Inbred BALB C ; Nanoparticles
Chemische Substanzen	Glycols ; Vaccines, DNA ; Cytokines
Sprache	Englisch
Erscheinungsdatum	2023-08-23
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	632565-8
ISSN	1532-4311 ; 0882-0139
ISSN (online)	1532-4311
ISSN	0882-0139
DOI	10.1080/08820139.2023.2243979
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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