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  1. Article ; Online: ENTREVISTA COM MARIA LIGIA COELHO PRADO

    Mary Anne Junqueira / Vitória Rodrigues e Silva

    Diálogos, Vol 8, Iss

    2017  Volume 2

    Abstract: Pergunta: A História é uma paixão antiga? Como foi sua formação como historiadora? - Resposta: Desde que me lembro, sempre gostei de História. Lembro-me de estudar História com prazer já no curso primário. Nos antigos Ginásio e Colegial também. Tive uma ... ...

    Abstract Pergunta: A História é uma paixão antiga? Como foi sua formação como historiadora? - Resposta: Desde que me lembro, sempre gostei de História. Lembro-me de estudar História com prazer já no curso primário. Nos antigos Ginásio e Colegial também. Tive uma professora nesse período, Julita Scarano, a quem muito admirava e que muito me ensinou. Mas a paixão desabrochou quando entrei para o curso de História da USP. Isto aconteceu em 1968, ano extraordinário e emblemático. Estava com 27 anos e havia feito, portanto, uma escolha tardia. Era mãe de três filhos, o mais novo dos quais mal tinha completado três meses, o que anunciava dificuldades especiais para cumprir todos os requisitos do curso. No entanto, completei-o, em 1971, nos 4 anos previstos, porque foi amor à primeira vista. Encantava-me o prédio, com seus espaços amplos, que davam uma sensação de liberdade e convidavam ao convívio. Entusiasmava-me com as disciplinas, com os professores, com os colegas. Quantas vezes pensei como seria bom se o curso não terminasse em quatro anos, que durasse uma eternidade.
    Keywords História ; Latin America. Spanish America ; F1201-3799 ; Social sciences (General) ; H1-99
    Language Portuguese
    Publishing date 2017-07-01T00:00:00Z
    Publisher Universidade Estadual de Maringá
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Entrevista com Maria Ligia Coelho Prado

    Mary Junqueira / Vitória Rodrigues e Silva

    Diálogos, Vol 8, Iss 2, Pp 65-

    2010  Volume 79

    Keywords Latin America. Spanish America ; F1201-3799 ; Social sciences (General) ; H1-99
    Language Portuguese
    Publishing date 2010-05-01T00:00:00Z
    Publisher Universidade Estadual de Maringá
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Aptamer-based therapy for fighting biofilm-associated infections.

    Sousa, Ana Margarida / Ferreira, Débora / Rodrigues, Lígia Raquel / Pereira, Maria Olívia

    Journal of controlled release : official journal of the Controlled Release Society

    2024  Volume 367, Page(s) 522–539

    Abstract: Biofilms are key players in the pathogenesis of most of chronic infections associated with host tissue or fluids and indwelling medical devices. These chronic infections are hard to be treated due to the increased biofilms tolerance towards antibiotics ... ...

    Abstract Biofilms are key players in the pathogenesis of most of chronic infections associated with host tissue or fluids and indwelling medical devices. These chronic infections are hard to be treated due to the increased biofilms tolerance towards antibiotics in comparison to planktonic (or free living) cells. Despite the advanced understanding of their formation and physiology, biofilms continue to be a challenge and there is no standardized therapeutic approach in clinical practice to eradicate them. Aptamers offer distinctive properties, including excellent affinity, selectivity, stability, making them valuable tools for therapeutic purposes. This review explores the flexibility and designability of aptamers as antibiofilm drugs but, importantly, as targeting tools for diverse drug and delivery systems. It highlights specific examples of application of aptamers in biofilms of diverse species according to different modes of action including inhibition of motility and adhesion, blocking of quorum sensing molecules, and dispersal of biofilm-cells to planktonic state. Moreover, it discusses the limitations and challenges that impaired an increased success of the use of aptamers on biofilm management, as well as the opportunities related to aptamers modifications that can significantly expand their applicability on the biofilm field.
    MeSH term(s) Humans ; Persistent Infection ; Biofilms ; Quorum Sensing ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Oligonucleotides
    Chemical Substances Anti-Bacterial Agents ; Oligonucleotides
    Language English
    Publishing date 2024-02-03
    Publishing country Netherlands
    Document type Review ; Journal Article
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2024.01.061
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cornélio Pires e o mundo caipira relido e encenado por Soffredini

    Lígia Rodrigues Balista

    Estudos Sociedade e Agricultura, Vol 28, Iss 3, Pp 594-

    2020  Volume 623

    Abstract: O artigo aborda a relação entre dois autores de tempos distintos que pensaram o mundo rural brasileiro: Cornélio Pires (1884-1954) e Carlos Alberto Soffredini (1939-2001). Para isso, analisa tanto a importância da figura de Cornélio Pires na difusão ... ...

    Abstract O artigo aborda a relação entre dois autores de tempos distintos que pensaram o mundo rural brasileiro: Cornélio Pires (1884-1954) e Carlos Alberto Soffredini (1939-2001). Para isso, analisa tanto a importância da figura de Cornélio Pires na difusão sobre o mundo rural na virada do século quanto algumas de suas criações no campo da música caipira e da literatura. Pires é um dos autores de referência ao trabalho de Soffredini, que revisita sua obra no espetáculo A estrambótica aventura da música caipira, de 1990 – analisado no intuito de compreender a representação de caipira produzida nessa peça, a releitura feita do trabalho de Pires e alguns impactos das escolhas dessa encenação no contexto do final do século XX no Brasil.
    Keywords cornélio pires ; carlos alberto soffredini ; cultura caipira ; literatura brasileira ; cultura popular ; Agriculture (General) ; S1-972 ; Land use ; HD101-1395.5 ; Agricultural industries ; HD9000-9495
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Universidade Federal Rural do Rio de Janeiro
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Challenges in the Heterologous Production of Furanocoumarins in Escherichia coli

    Joana L. Rodrigues / Daniela Gomes / Lígia R. Rodrigues

    Molecules, Vol 27, Iss 7230, p

    2022  Volume 7230

    Abstract: Coumarins and furanocoumarins are plant secondary metabolites with known biological activities. As they are present in low amounts in plants, their heterologous production emerged as a more sustainable and efficient approach to plant extraction. Although ...

    Abstract Coumarins and furanocoumarins are plant secondary metabolites with known biological activities. As they are present in low amounts in plants, their heterologous production emerged as a more sustainable and efficient approach to plant extraction. Although coumarins biosynthesis has been positively established, furanocoumarin biosynthesis has been far more challenging. This study aims to evaluate if Escherichia coli could be a suitable host for furanocoumarin biosynthesis. The biosynthetic pathway for coumarins biosynthesis in E. coli was effectively constructed, leading to the production of umbelliferone, esculetin and scopoletin (128.7, 17.6, and 15.7 µM, respectively, from tyrosine). However, it was not possible to complete the pathway with the enzymes that ultimately lead to furanocoumarins production. Prenyltransferase, psoralen synthase, and marmesin synthase did not show any activity when expressed in E. coli . Several strategies were tested to improve the enzymes solubility and activity with no success, including removing potential N -terminal transit peptides and expression of cytochrome P450 reductases, chaperones and/or enzymes to increase dimethylallylpyrophosphate availability. Considering the results herein obtained, E. coli does not seem to be an appropriate host to express these enzymes. However, new alternative microbial enzymes may be a suitable option for reconstituting the furanocoumarins pathway in E. coli . Nevertheless, until further microbial enzymes are identified, Saccharomyces cerevisiae may be considered a preferred host as it has already been proven to successfully express some of these plant enzymes.
    Keywords coumarins biosynthesis ; Escherichia coli ; heterologous production ; umbelliferone ; scopoletin ; esculetin ; Organic chemistry ; QD241-441
    Subject code 572 ; 580
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Challenges in the Heterologous Production of Furanocoumarins in

    Rodrigues, Joana L / Gomes, Daniela / Rodrigues, Lígia R

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 21

    Abstract: Coumarins and furanocoumarins are plant secondary metabolites with known biological activities. As they are present in low amounts in plants, their heterologous production emerged as a more sustainable and efficient approach to plant extraction. Although ...

    Abstract Coumarins and furanocoumarins are plant secondary metabolites with known biological activities. As they are present in low amounts in plants, their heterologous production emerged as a more sustainable and efficient approach to plant extraction. Although coumarins biosynthesis has been positively established, furanocoumarin biosynthesis has been far more challenging. This study aims to evaluate if
    MeSH term(s) Furocoumarins/chemistry ; Escherichia coli/metabolism ; Coumarins/metabolism ; Scopoletin/metabolism ; Plants/metabolism
    Chemical Substances Furocoumarins ; Coumarins ; Scopoletin (KLF1HS0SXJ)
    Language English
    Publishing date 2022-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27217230
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 81: Show issues

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  7. Article: Heterologous production of chondroitin.

    Couto, Márcia R / Rodrigues, Joana L / Rodrigues, Lígia R

    Biotechnology reports (Amsterdam, Netherlands)

    2022  Volume 33, Page(s) e00710

    Abstract: Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the ... ...

    Abstract Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the search for alternative methods and sources to obtain this compound. Several pathogenic bacteria naturally produce chondroitin-like polysaccharides through well-known pathways and, therefore, have been the basis for numerous studies that aim to produce chondroitin using non-pathogenic hosts. However, the yields obtained are not enough to meet the high demand for this glycosaminoglycan. Metabolic engineering strategies have been used to construct improved heterologous hosts. The identification of metabolic bottlenecks and regulation points, and the screening for efficient enzymes are key points for constructing microbial cell factories with improved chondroitin yields to achieve industrial CS production. The recent advances on enzymatic and microbial strategies to produce non-animal chondroitin are herein reviewed. Challenges and prospects for future research are also discussed.
    Language English
    Publishing date 2022-02-10
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2022.e00710
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Editorial: Microorganisms and their derivatives for cancer therapy.

    Zhang, Yunlei / Rodrigues, Lígia R / Cao, Zhenping / Li, Juanjuan

    Frontiers in bioengineering and biotechnology

    2023  Volume 11, Page(s) 1137341

    Language English
    Publishing date 2023-01-13
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2023.1137341
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Biosynthesis and heterologous production of furanocoumarins: perspectives and current challenges.

    Rodrigues, Joana L / Rodrigues, Lígia R

    Natural product reports

    2020  Volume 38, Issue 5, Page(s) 869–879

    Abstract: Covering: up to October 2020 Furanocoumarins are plant secondary metabolites used to treat several skin disorders, such as psoriasis and vitiligo, and also with other potential therapeutic activities. Furanocoumarins are extracted from plants where they ... ...

    Abstract Covering: up to October 2020 Furanocoumarins are plant secondary metabolites used to treat several skin disorders, such as psoriasis and vitiligo, and also with other potential therapeutic activities. Furanocoumarins are extracted from plants where they accumulate in low amounts over long growth periods. In addition, their extraction and purification are difficult in an environmentally unfriendly and expensive process. Hence, new sustainable and greener production schemes able to overcome such limitations ought to be developed. While the heterologous production of simple coumarins has been demonstrated, the biosynthesis of more complex furanocoumarins remains greatly unexplored. Although several important steps of the pathway have been elucidated in the last decade, the complete pathway has not been completely unravelled. In this paper, we review the natural conversion of amino acids into furanocoumarins, as well as the heterologous expression of each enzyme of the pathway. We also explore the challenges that need to be addressed so that their heterologous production can become a viable alternative.
    MeSH term(s) Furocoumarins/biosynthesis ; Molecular Structure ; Phytochemicals/biosynthesis ; Plants/metabolism ; Secondary Metabolism
    Chemical Substances Furocoumarins ; Phytochemicals
    Language English
    Publishing date 2020-11-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2002546-4
    ISSN 1460-4752 ; 0265-0568
    ISSN (online) 1460-4752
    ISSN 0265-0568
    DOI 10.1039/d0np00074d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Engineering A-type Dye-Decolorizing Peroxidases by Modification of a Conserved Glutamate Residue.

    Hermann, Enikö / Rodrigues, Carolina F / Martins, Lígia O / Peterbauer, Clemens / Oostenbrink, Chris

    Chembiochem : a European journal of chemical biology

    2024  , Page(s) e202300872

    Abstract: Dye-decolorizing peroxidases (DyPs) are recently identified microbial enzymes that have been used in several Biotechnology applications from wastewater treatment to lignin valorization. However, their properties and mechanism of action still have many ... ...

    Abstract Dye-decolorizing peroxidases (DyPs) are recently identified microbial enzymes that have been used in several Biotechnology applications from wastewater treatment to lignin valorization. However, their properties and mechanism of action still have many open questions. Their heme-containing active site is buried by three conserved flexible loops with a putative role in modulating substrate access and enzyme catalysis. Here, we investigated the role of a conserved glutamate residue in stabilizing interactions in loop 2 of A-type DyPs. First, we did site saturation mutagenesis of this residue, replacing it with all possible amino acids in bacterial DyPs from Bacillus subtilis (BsDyP) and from Kitasatospora aureofaciens (KaDyP1), the latter being characterized here for the first time. We screened the resulting libraries of variants for activity towards ABTS and identified variants with increased catalytic efficiency. The selected variants were purified and characterized for activity and stability. We furthermore used Molecular Dynamics simulations to rationalize the increased catalytic efficiency and found that the main reason is the electron channeling becoming easier from surface-exposed tryptophans. Based on our findings, we also propose that this glutamate could work as a pH switch in the wild-type enzyme, preventing intracellular damage.
    Language English
    Publishing date 2024-02-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202300872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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