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  1. Article: The conjugated plasma proteins of the American cockroach. I. The normal state.

    SIAKOTOS, A N

    The Journal of general physiology

    2000  Volume 43, Page(s) 999–1013

    Abstract: Five protein fractions have been separated by paper electrophoresis from the plasma of the American cockroach. With the utilization of various staining procedures several of the plasma fractions were shown to be conjugated proteins. Two of these ( ... ...

    Abstract Five protein fractions have been separated by paper electrophoresis from the plasma of the American cockroach. With the utilization of various staining procedures several of the plasma fractions were shown to be conjugated proteins. Two of these (fractions II and IV) are readily identifiable by their phospholipid, carbohydrate, and protein composition. A third conjugated protein, fraction III, is characterized by its high neutral lipid and sterol content. This lipoprotein is also sex-specific. Another fraction (I) contains neutral lipid, sterol, and protein but electrophoretically is more mobile than fraction III. Fraction V, the last and least mobile of the normally occurring proteins, possesses electrophoretic properties similar to human fibrinogen.
    MeSH term(s) Animals ; Blood Proteins ; Female ; Humans ; Insecta ; Lipids ; Male ; Periplaneta ; Phospholipids ; Proteins
    Chemical Substances Blood Proteins ; Lipids ; Phospholipids ; Proteins
    Language English
    Publishing date 2000-12-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3118-5
    ISSN 1540-7748 ; 0022-1295
    ISSN (online) 1540-7748
    ISSN 0022-1295
    DOI 10.1085/jgp.43.5.999
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The conjugated plasma proteins of the American cockroach. II. Changes during the molting and clotting processes.

    SIAKOTOS, A N

    The Journal of general physiology

    2000  Volume 43, Page(s) 1015–1030

    Abstract: Evidence has been presented for the possible transport function of conjugated roach plasma proteins during molting. Extensive changes in these proteins are evident during the premolt stage. The remainder of the instar appears to be devoted to a gradual ... ...

    Abstract Evidence has been presented for the possible transport function of conjugated roach plasma proteins during molting. Extensive changes in these proteins are evident during the premolt stage. The remainder of the instar appears to be devoted to a gradual return to the intermolt stage. This recovery process is characterized principally by a transformation of less mobile lipoproteins to lipoproteins of higher electrophoretic mobilities and may be indicative of a lipoprotein-lipase reaction in insects. This transformation also appears to give rise to a second sex-specific lipoprotein. Significant changes in the glycoproteins at the premolt and ecdysial stages may indicate transport of carbohydrate for storage or utilization. A total of six fractions has been shown to occur in roach plasma in the period from one molt to the next. The process of clotting appeared to be concerned primarily with the blood lipoproteins. These protein fractions reacted to form two new lipoproteins, one of which was the relatively insoluble coagulum network. The second major protein fraction appearing as a result of the coagulation process also was a lipoprotein which contained a lower concentration of carbohydrate than the clot. A possible method of assaying or screening new anticoagulants for insect blood is proposed.
    MeSH term(s) Animals ; Biological Transport ; Blood Proteins ; Female ; Humans ; Insecta ; Lipoproteins ; Male ; Molting ; Periplaneta ; Proteins
    Chemical Substances Blood Proteins ; Lipoproteins ; Proteins
    Language English
    Publishing date 2000-12-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3118-5
    ISSN 1540-7748 ; 0022-1295
    ISSN (online) 1540-7748
    ISSN 0022-1295
    DOI 10.1085/jgp.43.5.1015
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  3. Article: GLASS-BREAKING PLIERS FOR THE PREPARATION OF GLASS KNIVES USED IN ULTRAMICROTOMY.

    SIAKOTOS, A N / MAIOLATESI, E

    Stain technology

    2003  Volume 39, Page(s) 171–172

    MeSH term(s) Glass ; Household Articles ; Microtomy
    Language English
    Publishing date 2003-08-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 204773-1
    ISSN 0038-9153
    ISSN 0038-9153
    DOI 10.3109/10520296409061224
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  4. Article: Morphological and biochemical abnormalities in a model of retinal degeneration: Canine ceroid-lipofuscinosis (CCL).

    Armstrong, D / Neville, H / Siakotos, A / Wilson, B / Wehling, C / Koppang, N

    Neurochemistry international

    2009  Volume 1C, Page(s) 405–426

    Abstract: Human ceroid-lipofuscinosis is marked by blindness, dementia, ataxia, and premature death. A canine model for this disease exists in English setters whose clinical, pathological and biochemical changes resemble the human disorder. In both syndromes, ... ...

    Abstract Human ceroid-lipofuscinosis is marked by blindness, dementia, ataxia, and premature death. A canine model for this disease exists in English setters whose clinical, pathological and biochemical changes resemble the human disorder. In both syndromes, autofluorescent lipopigments, i.e.; lipofuscin and ceroid ("granular", "fingerprint" and/or "curvilinear bodies") are found in the nervous system, viscera, retina, and pigment epithelium (RPE). Retinal neurons of affected animals between 6 and 22 months of age, contain a variety of abnormal intracellular pigment inclusions. Pigment epithelial cells also contain distinctive cytosomes. Electroretinograms from affected animals showed a reduction in b-wave amplitude. Leukocyte, retinal, and RPE peroxidases, were decreased in affected animals, and also showed age-related changes. In the normal canine eye, peroxidase was associated with fractions containing plasma membranes and melanolysosomes. Improved fractionation techniques localized normal peroxidase to "heavy" fractions (1.24-1.28 g/ml), and peroxidase was decreased in these fractions in CCL animals. A new particle containing hexosaminidase, galactosidase, and acid lipase was observed in affected animals. When retinal homogenates from CCL dogs were injected into the vitreous of rabbit eyes they completely abolished the ERG recording. No such change was observed with homogenates from unaffected animals. The accumulation of large numbers of dense bodies in the retina and RPE in dogs with CCL, along with a decrease in peroxidase, suggests an impairment of degradative mechanisms. Furthermore, ceroid appears to be cytotoxic to the retina and RPE. The relationship of these cytotoxic properties to the accumulation of ceroid in the eye, is the subject of our future research.
    Language English
    Publishing date 2009-11-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 283190-9
    ISSN 1872-9754 ; 0197-0186
    ISSN (online) 1872-9754
    ISSN 0197-0186
    DOI 10.1016/0197-0186(80)90076-5
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  5. Article: POLYSACCHARIDE AND GLYCOPROTEIN FORMATION IN THE COCKROACH. I. IDENTITY AND TITER OF BOUND MONOSACCHARIDES.

    LIPKE, H / GRAINGER, M M / SIAKOTOS, A N

    The Journal of biological chemistry

    2002  Volume 240, Page(s) 594–600

    MeSH term(s) Amino Acids ; Animals ; Arabinose ; Biochemical Phenomena ; Biochemistry ; Carbohydrate Metabolism ; Chromatography ; Cockroaches ; Disaccharides ; Galactose ; Glucose ; Glycogen ; Glycoproteins ; Hexosamines ; Insecta ; Ion Exchange Resins ; Mannose ; Metamorphosis, Biological ; Monosaccharides ; Polysaccharides ; Proteins/metabolism ; Research ; Xylose
    Chemical Substances Amino Acids ; Disaccharides ; Glycoproteins ; Hexosamines ; Ion Exchange Resins ; Monosaccharides ; Polysaccharides ; Proteins ; Glycogen (9005-79-2) ; Xylose (A1TA934AKO) ; Arabinose (B40ROO395Z) ; Glucose (IY9XDZ35W2) ; Mannose (PHA4727WTP) ; Galactose (X2RN3Q8DNE)
    Language English
    Publishing date 2002-11-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
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  6. Article: Canine hereditary ceroid-lipofuscinosis: evidence for a defect in the carnitine biosynthetic pathway.

    Katz, M L / Siakotos, A N

    American journal of medical genetics

    1995  Volume 57, Issue 2, Page(s) 266–271

    Abstract: ... that contains an epsilon-N-trimethyllysine (TML) residue. TML is a precursor in carnitine biosynthesis ...

    Abstract The ceroid-lipofuscinoses are a group of autosomal-recessive hereditary lysosomal storage diseases that have been characterized in humans and other mammalian species. In a canine model for the juvenile form of the human disease, a major constituent of the storage bodies is the subunit c protein of mitochondrial ATP synthase that contains an epsilon-N-trimethyllysine (TML) residue. TML is a precursor in carnitine biosynthesis. To determine whether accumulation of the TML-containing protein could result from a defect in the carnitine biosynthetic pathway, plasma carnitine and trimethyllysine levels were measured in homozygous affected, heterozygous carriers, and in normal dogs. When compared top normal animals, mean carnitine levels were reduced by 67% in affected and 50% in carrier dogs. Mean plasma TML levels were elevated almost 50% above control levels in the carriers, but were decreased by approximately 25% in the affected animals. The changes in plasma carnitine and TML levels in the carriers are consistent with the possibility that the disease involves a defect in the carnitine biosynthetic pathway. Secondary effects of the disease process may account for the apparently contradictory decrease in plasma TML levels in affected animals.
    MeSH term(s) Animals ; Biomarkers/blood ; Carnitine/metabolism ; Dog Diseases ; Dogs ; Genetic Carrier Screening ; Homozygote ; Humans ; Lysine/analogs & derivatives ; Lysine/blood ; Macromolecular Substances ; Mammals ; Mitochondria/enzymology ; Neuronal Ceroid-Lipofuscinoses/genetics ; Neuronal Ceroid-Lipofuscinoses/metabolism ; Neuronal Ceroid-Lipofuscinoses/veterinary ; Proton-Translocating ATPases/metabolism ; Reference Values
    Chemical Substances Biomarkers ; Macromolecular Substances ; trimethyllysine (3YGF0495O2) ; Proton-Translocating ATPases (EC 3.6.3.14) ; Lysine (K3Z4F929H6) ; Carnitine (S7UI8SM58A)
    Language English
    Publishing date 1995-06-05
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 133387-2
    ISSN 0148-7299
    ISSN 0148-7299
    DOI 10.1002/ajmg.1320570231
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  7. Article: The isolation of nuclei from normal human and bovine brain.

    Siakotos, A N

    Methods in enzymology

    1974  Volume 31, Page(s) 452–457

    MeSH term(s) Animals ; Brain/ultrastructure ; Cattle ; Cell Fractionation/methods ; Cell Nucleus/ultrastructure ; Centrifugation, Density Gradient ; Chromatography, Gel ; Humans ; Microscopy, Phase-Contrast ; Neuroglia/cytology ; Neuroglia/ultrastructure ; Species Specificity ; Ultracentrifugation
    Language English
    Publishing date 1974
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ISSN 0076-6879
    ISSN 0076-6879
    DOI 10.1016/0076-6879(74)31051-8
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  8. Article: The isolation of endothelial cells from normal human and bovine brain.

    Siakotos, A N

    Methods in enzymology

    1974  Volume 32, Page(s) 717–722

    MeSH term(s) Animals ; Brain/cytology ; Brain/ultrastructure ; Cattle ; Centrifugation ; Chromatography ; Endothelium/cytology ; Endothelium/ultrastructure ; Filtration ; Glass ; Humans ; Methods ; Microscopy, Electron ; Microscopy, Phase-Contrast ; Species Specificity
    Language English
    Publishing date 1974
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ISSN 0076-6879
    ISSN 0076-6879
    DOI 10.1016/0076-6879(74)32074-5
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  9. Article: Procedures for the isolation of brain lipopigments: ceroid and lipofuscin.

    Siakotos, A N

    Methods in enzymology

    1974  Volume 31, Page(s) 478–485

    MeSH term(s) Aging ; Animals ; Brain/cytology ; Brain/growth & development ; Brain/ultrastructure ; Brain Chemistry ; Centrifugation, Density Gradient ; Dextrans ; Dogs ; Humans ; Lipids/isolation & purification ; Methods ; Microscopy, Fluorescence ; Pigments, Biological/isolation & purification ; Time Factors ; Ultracentrifugation
    Chemical Substances Dextrans ; Lipids ; Pigments, Biological
    Language English
    Publishing date 1974
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ISSN 0076-6879
    ISSN 0076-6879
    DOI 10.1016/0076-6879(74)31053-1
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  10. Article: Assessment of dietary therapies in a canine model of Batten disease.

    Siakotos, A N / Hutchins, G D / Farlow, M R / Katz, M L

    European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society

    2001  Volume 5 Suppl A, Page(s) 151–156

    Abstract: The neuronal ceroid lipofuscinoses (NCLs) are inherited neurodegenerative diseases that occur in a number of animal species, including dogs. A study was conducted to determine whether the resupply of nutrients lost in NCL English Setter dogs would modify ...

    Abstract The neuronal ceroid lipofuscinoses (NCLs) are inherited neurodegenerative diseases that occur in a number of animal species, including dogs. A study was conducted to determine whether the resupply of nutrients lost in NCL English Setter dogs would modify the course of the disease. Carnitine and polyunsaturated fatty acids have been reported to be reduced in NCL English Setters. Therefore, the normal laboratory diets of NCL dogs were supplemented with carnitine, fish oil and corn oil and the disease progression was compared with that of an untreated litter mate. The following specific prognostic indicators of NCL were monitored: cognitive function, brain atrophy, brain glucose metabolism and lifespan. Carnitine, with or without lipid supplements, dramatically delayed the progression of cognitive decline in NCL dogs. When fish oil and corn oil only were supplied, brain atrophy was reduced. A combination of all three supplements preserved cognitive function and increased lifespan by 10%. However, brain glucose hypometabolism and cerebral atrophy were not reduced. The results in this study indicated that the effectiveness of therapeutic interventions can be assessed by non-invasive methods at a relatively early stage of the disease process. Our study suggests that dietary supplementation with carnitine is a promising new approach for delaying or preventing the cognitive decline in dogs, and perhaps, with human NCL patients.
    MeSH term(s) Animals ; Carnitine/pharmacology ; Cerebral Ventricles/pathology ; Corn Oil/pharmacology ; Disease Models, Animal ; Dogs ; Fish Oils/pharmacology ; Glucose/metabolism ; Magnetic Resonance Imaging ; Neuronal Ceroid-Lipofuscinoses/diet therapy ; Neuronal Ceroid-Lipofuscinoses/pathology ; Tomography, Emission-Computed
    Chemical Substances Fish Oils ; Corn Oil (8001-30-7) ; Glucose (IY9XDZ35W2) ; Carnitine (S7UI8SM58A)
    Language English
    Publishing date 2001-12-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1397146-3
    ISSN 1090-3798
    ISSN 1090-3798
    DOI 10.1053/ejpn.2000.0453
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 641: Show issues

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