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Article: Social perception of young adults prolongs the lifespan of aged Drosophila.

Cho, Li-Chun / Yu, Chih-Chieh / Kao, Chih-Fei

2021 Volume 7, Issue 1, Page(s) 21

Abstract: Lifespan is modulated at distinct levels by multiple factors, including genetic backgrounds, the environment, behavior traits, metabolic status, and more interestingly, sensory perceptions. However, the effects of social perception between individuals ... ...

Abstract	Lifespan is modulated at distinct levels by multiple factors, including genetic backgrounds, the environment, behavior traits, metabolic status, and more interestingly, sensory perceptions. However, the effects of social perception between individuals living in the same space remain less clear. Here, we used the Drosophila model to study the influences of social perception on the lifespan of aged fruit flies. We found the lifespan of aged Drosophila is markedly prolonged after being co-housed with young adults of the same gender. Moreover, the changes of lifespan were affected by several experimental contexts: (1) the ratios of aged and young adults co-housed, (2) the chronological ages of two populations, and (3) the integrity of sensory modalities. Together, we hypothesize the chemical/physical stimuli derived from the interacting young adults are capable of interfering with the physiology and behavior of aged flies, ultimately leading to the alteration of lifespan.
Language	English
Publishing date	2021-09-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2836493-4
ISSN	2056-3973
ISSN	2056-3973
DOI	10.1038/s41514-021-00073-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Neuroprotective Effect of NO-Delivery Dinitrosyl Iron Complexes (DNICs) on Amyloid Pathology in the Alzheimer's Disease Cell Model.

Chuang, Wen-Han / Chou, Yu-Ting / Chen, Yi-Hong / Kuo, Ting-Han / Liaw, Wen-Feng / Lu, Tsai-Te / Kao, Chih-Fei / Wang, Yun-Ming

ACS chemical neuroscience

2023 Volume 14, Issue 16, Page(s) 2922–2934

Abstract: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairment, memory loss, and behavioral deficits. β- ... ...

Abstract	Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairment, memory loss, and behavioral deficits. β-amyloid
MeSH term(s)	Humans ; Nitric Oxide/metabolism ; Neuroprotective Agents/pharmacology ; Alzheimer Disease/drug therapy ; Iron/metabolism ; Amyloid beta-Peptides
Chemical Substances	dinitrosyl iron complex (68586-27-6) ; Nitric Oxide (31C4KY9ESH) ; Neuroprotective Agents ; Iron (E1UOL152H7) ; Amyloid beta-Peptides
Language	English
Publishing date	2023-08-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1948-7193
ISSN (online)	1948-7193
DOI	10.1021/acschemneuro.3c00348
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Article: One-step-one-pot hydrothermally derived metal-organic-framework-nanohybrids for integrated point-of-care diagnostics of SARS-CoV-2 viral antigen/pseudovirus utilizing electrochemical biosensor chip.

Palanisamy, Sathyadevi / Lee, Li-Yun / Kao, Chih-Fei / Chen, Wen-Liang / Wang, Hsiang-Ching / Shen, San-Tai / Jian, Jhih-Wei / Yuan, Shyng-Shiou F / Kung, Yu-An / Wang, Yun-Ming

Sensors and actuators. B, Chemical

2023 Volume 390, Page(s) 133960

Abstract: The COVID-19 pandemic has become a global catastrophe, affecting the health and economy of the human community. It is required to mitigate the impact of pandemics by developing rapid molecular diagnostics for SARS-CoV-2 virus detection. In this context, ... ...

Abstract	The COVID-19 pandemic has become a global catastrophe, affecting the health and economy of the human community. It is required to mitigate the impact of pandemics by developing rapid molecular diagnostics for SARS-CoV-2 virus detection. In this context, developing a rapid point-of-care (POC) diagnostic test is a holistic approach to the prevention of COVID-19. In this context, this study aims at presenting a real-time, biosensor chip for improved molecular diagnostics including recombinant SARS-CoV-2 spike glycoprotein and SARS-CoV-2 pseudovirus detection based on one-step-one-pot hydrothermally derived CoFeBDCNH
Language	English
Publishing date	2023-05-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1021505-0
ISSN	0925-4005
ISSN	0925-4005
DOI	10.1016/j.snb.2023.133960
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Article ; Online: Reverse genetics by loss-of-function mosaic analysis in Drosophila.

Kao, Chih-Fei / Lee, Tzumin

Cold Spring Harbor protocols

2013 Volume 2013, Issue 1

Abstract: Genetic mosaics in Drosophila typically involve derivation of homozygous daughter cells from heterozygous precursors through mitotic recombination. MARCM (mosaic analysis with a repressible cell marker) couples loss of heterozygosity with derepression of ...

Abstract	Genetic mosaics in Drosophila typically involve derivation of homozygous daughter cells from heterozygous precursors through mitotic recombination. MARCM (mosaic analysis with a repressible cell marker) couples loss of heterozygosity with derepression of a marker gene, permitting unique labeling of specific homozygous daughter cells. The generation of GAL80-minus homozygous daughter cells in otherwise heterozygous tissues allows GAL4-dependent activation of upstream activation sequence (UAS)-reporter specifically in the homozygous cells of interest. To make MARCM clones, organisms must carry at least five genetic elements (flippase [FLP], flippase recognition targets [FRTs], tubP-GAL80, GAL4, and UAS-marker) in specific configurations. One major application of MARCM, as described here, is to study cell-autonomous function(s) of a gene within single cells or a group of cells in otherwise unperturbed organisms. A mutation of interest distal to one FRT site is put in trans to a tubP-GAL80-containing chromosome arm that carries the same FRT. The resulting MARCM clones, which are negative for tubP-GAL80 and thus specifically marked, will become homozygous for the mutation in otherwise heterozygous organisms. By including a UAS-transgene, one can perform rescue experiments in the mutant MARCM clones. Conversely, if the mutation is placed on the same chromosome arm as tubP-GAL80, MARCM-labeled cells will be homozygous wild-type and may lie adjacent to sister cells that are homozygous mutant. This variant, called reverse MARCM, allows one to determine non-cell-autonomous effects of a mutation.
MeSH term(s)	Animals ; Drosophila/genetics ; Homozygote ; Mosaicism ; Mutation ; Reverse Genetics/methods
Language	English
Publishing date	2013-01-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot071670
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Article ; Online: Genetic mosaic screens in Drosophila mushroom bodies.

Kao, Chih-Fei / Lee, Tzumin

Cold Spring Harbor protocols

2013 Volume 2013, Issue 1

Abstract	Genetic mosaics in Drosophila typically involve derivation of homozygous daughter cells from heterozygous precursors through mitotic recombination. MARCM (mosaic analysis with a repressible cell marker) couples loss of heterozygosity with derepression of a marker gene, permitting unique labeling of specific homozygous daughter cells. The generation of GAL80-minus homozygous daughter cells in otherwise heterozygous tissues allows GAL4-dependent activation of upstream activation sequence (UAS)-reporter specifically in the homozygous cells of interest. To make MARCM clones, organisms must carry at least five genetic elements (flippase [FLP], flippase recognition targets [FRTs], tubP-GAL80, GAL4, and UAS-marker) in specific configurations. In neurons whose progenitors can be efficiently targeted for mitotic recombination, genetic mosaic screens can be used to systematically uncover cell-autonomous genes that are required for development or function. This technique involves the generation of numerous FRT lines carrying various independent mutations, followed by derivation and phenotypic analysis of MARCM clones using these mutant FRT lines in combination with an MARCM-enabling stock that carries all the other genetic elements required for MARCM. Mutants of interest are recovered based on the MARCM phenotypes, which are imaged live using diverse fluorescent markers. Mutant genes that underlie the phenotypes of interest can then be identified by conventional genetics including derivation and analysis of series of recombinant chromosomes. Besides chemical mutagenesis, genes on a particular FRT chromosome may be randomly disrupted by P element insertion. This protocol describes procedures specifically used for genetic mosaic screens in the mushroom bodies (MBs).
MeSH term(s)	Animals ; Drosophila/genetics ; Genetic Testing/methods ; Mosaicism ; Mushroom Bodies ; Mutation
Language	English
Publishing date	2013-01-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot071688
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Article ; Online: Generation of standard wild-type MARCM clones for analysis of drosophila brain development.

Kao, Chih-Fei / Lee, Tzumin

Cold Spring Harbor protocols

2012 Volume 2012, Issue 12

Abstract: The generation and analysis of clones of cells with a genotype different from the rest of an organism has been used for studying molecular mechanisms underlying development. Genetic mosaics in Drosophila typically involve derivation of homozygous ... ...

Abstract	The generation and analysis of clones of cells with a genotype different from the rest of an organism has been used for studying molecular mechanisms underlying development. Genetic mosaics in Drosophila typically involve derivation of homozygous daughter cells from heterozygous precursors through mitotic recombination. MARCM (mosaic analysis with a repressible cell marker) couples loss of heterozygosity with derepression of a marker gene. This permits unique labeling of specific homozygous daughter cells, and thus makes mosaic analysis possible in the complex nervous system. The principle of MARCM involves the generation of GAL80-minus homozygous daughter cells in otherwise heterozygous tissues, therefore allowing GAL4-dependent activation of upstream activation sequence (UAS)-reporter specifically in the homozygous cells of interest. To make MARCM clones, it is necessary to generate organisms carrying at least five genetic elements (flippase [FLP], flippase recognition targets [FRTs], tubP-GAL80, GAL4, and UAS-marker) in specific configurations. Induction of FLP in neural precursors can be temporally controlled using a heat-shock promoter or spatially regulated using a tissue-specific promoter. Mitotic recombination in a neuroblast (NB) may yield a multicellular NB clone (progeny of the renewed NB) or a two-cell clone (progeny of the derived ganglion mother cell [GMC]). Single-cell clones can be obtained following mitotic recombination in GMCs. Phenotypic analysis of mosaic brains can be greatly simplified using a GAL4 driver that is only expressed in neurons of interest. One can also mark different subcellular structures of the clones using distinct UAS reporters. This protocol outlines the steps involved in standard MARCM analysis.
MeSH term(s)	Animals ; Brain/embryology ; Drosophila/embryology ; Drosophila/genetics ; Entomology/methods ; Genotype ; Molecular Biology/methods ; Mosaicism
Language	English
Publishing date	2012-12-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot071662
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Article: Assessing the cognitive status of Drosophila by the value-based feeding decision.

Yu, Chih-Chieh / Chang, Ferng-Chang / Hong, Yong-Huei / Li, Jian-Chiuan / Chen, Po-Lin / Chen, Chun-Hong / Chiu, Tzai-Wen / Lu, Tsai-Te / Wang, Yun-Ming / Kao, Chih-Fei

NPJ aging and mechanisms of disease

2021 Volume 7, Issue 1, Page(s) 24

Abstract: Decision-making is considered an important aspect of cognitive function. Impaired decision-making is a consequence of cognitive decline caused by various physiological conditions, such as aging and neurodegenerative diseases. Here we exploited the value- ... ...

Abstract	Decision-making is considered an important aspect of cognitive function. Impaired decision-making is a consequence of cognitive decline caused by various physiological conditions, such as aging and neurodegenerative diseases. Here we exploited the value-based feeding decision (VBFD) assay, which is a simple sensory-motor task, to determine the cognitive status of Drosophila. Our results indicated the deterioration of VBFD is notably correlated with aging and neurodegenerative disorders. Restriction of the mushroom body (MB) neuronal activity partly blunted the proper VBFD. Furthermore, using the Drosophila polyQ disease model, we demonstrated the impaired VBFD is ameliorated by the dinitrosyl iron complex (DNIC-1), a novel and steady nitric oxide (NO)-releasing compound. Therefore we propose that the VBFD assay provides a robust assessment of Drosophila cognition and can be used to characterize additional neuroprotective interventions.
Language	English
Publishing date	2021-09-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2836493-4
ISSN	2056-3973
ISSN	2056-3973
DOI	10.1038/s41514-021-00075-6
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Article ; Online: Birth time/order-dependent neuron type specification.

Kao, Chih-Fei / Lee, Tzumin

Current opinion in neurobiology

2009 Volume 20, Issue 1, Page(s) 14–21

Abstract: Neurons derived from the same progenitor may acquire different fates according to their birth timing/order. To reveal temporally guided cell fates, we must determine neuron types as well as their lineage relationships and times of birth. Recent advances ... ...

Abstract	Neurons derived from the same progenitor may acquire different fates according to their birth timing/order. To reveal temporally guided cell fates, we must determine neuron types as well as their lineage relationships and times of birth. Recent advances in genetic lineage analysis and fate mapping are facilitating such studies. For example, high-resolution lineage analysis can identify each sequentially derived neuron of a lineage and has revealed abrupt temporal identity changes in diverse Drosophila neuronal lineages. In addition, fate mapping of mouse neurons made from the same pool of precursors shows production of specific neuron types in specific temporal patterns. The tools used in these analyses are helping to further our understanding of the genetics of neuronal temporal identity.
MeSH term(s)	Animals ; Cell Lineage/physiology ; Drosophila ; Mice ; Neurogenesis/physiology ; Neurons/physiology ; Time Factors
Language	English
Publishing date	2009-11-26
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1078046-4
ISSN	1873-6882 ; 0959-4388
ISSN (online)	1873-6882
ISSN	0959-4388
DOI	10.1016/j.conb.2009.10.017
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Article ; Online: Lineage analysis of Drosophila lateral antennal lobe neurons reveals notch-dependent binary temporal fate decisions.

Suewei Lin / Chih-Fei Kao / Hung-Hsiang Yu / Yaling Huang / Tzumin Lee

PLoS Biology, Vol 10, Iss 11, p e

2012 Volume 1001425

Abstract: Binary cell fate decisions allow the production of distinct sister neurons from an intermediate precursor. Neurons are further diversified based on the birth order of intermediate precursors. Here we examined the interplay between binary cell fate and ... ...

Abstract	Binary cell fate decisions allow the production of distinct sister neurons from an intermediate precursor. Neurons are further diversified based on the birth order of intermediate precursors. Here we examined the interplay between binary cell fate and birth-order-dependent temporal fate in the Drosophila lateral antennal lobe (lAL) neuronal lineage. Single-cell mapping of the lAL lineage by twin-spot mosaic analysis with repressible cell markers (ts-MARCM) revealed that projection neurons (PNs) and local interneurons (LNs) are made in pairs through binary fate decisions. Forty-five types of PNs innervating distinct brain regions arise in a stereotyped sequence; however, the PNs with similar morphologies are not necessarily born in a contiguous window. The LNs are morphologically less diverse than the PNs, and the sequential morphogenetic changes in the two pairs occur independently. Sanpodo-dependent Notch activity promotes and patterns the LN fates. By contrast, Notch diversifies PN temporal fates in a Sanpodo-dispensable manner. These pleiotropic Notch actions underlie the differential temporal fate specification of twin neurons produced by common precursors within a lineage, possibly by modulating postmitotic neurons' responses to Notch-independent transcriptional cascades.
Keywords	Biology (General) ; QH301-705.5
Subject code	590
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hierarchical deployment of factors regulating temporal fate in a diverse neuronal lineage of the Drosophila central brain.

Kao, Chih-Fei / Yu, Hung-Hsiang / He, Yisheng / Kao, Jui-Chun / Lee, Tzumin

Neuron

2012 Volume 73, Issue 4, Page(s) 677–684

Abstract: The anterodorsal projection neuron lineage of Drosophila melanogaster produces 40 neuronal types in a stereotypic order. Here we take advantage of this complete lineage sequence to examine the role of known temporal fating factors, including Chinmo and ... ...

Abstract	The anterodorsal projection neuron lineage of Drosophila melanogaster produces 40 neuronal types in a stereotypic order. Here we take advantage of this complete lineage sequence to examine the role of known temporal fating factors, including Chinmo and the Hb/Kr/Pdm/Cas transcriptional cascade, within this diverse central brain lineage. Kr mutation affects the temporal fate of the neuroblast (NB) itself, causing a single fate to be skipped, whereas Chinmo null only elicits fate transformation of NB progeny without altering cell counts. Notably, Chinmo operates in two separate windows to prevent fate transformation (into the subsequent Chinmo-indenpendent fate) within each window. By contrast, Hb/Pdm/Cas play no detectable role, indicating that Kr either acts outside of the cascade identified in the ventral nerve cord or that redundancy exists at the level of fating factors. Therefore, hierarchical fating mechanisms operate within the lineage to generate neuronal diversity in an unprecedented fashion.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Body Patterning ; Brain/cytology ; Cell Lineage/genetics ; Cell Lineage/physiology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/cytology ; Drosophila melanogaster/embryology ; Drosophila melanogaster/genetics ; Gene Expression Regulation, Developmental/genetics ; Gene Expression Regulation, Developmental/physiology ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Kruppel-Like Transcription Factors/genetics ; Kruppel-Like Transcription Factors/metabolism ; Mutation/genetics ; Nerve Tissue Proteins/genetics ; Neurons/physiology ; POU Domain Factors/genetics ; POU Domain Factors/metabolism ; Signal Transduction/genetics ; Signal Transduction/physiology ; Transcription Factors/metabolism
Chemical Substances	Chinmo protein, Drosophila ; DNA-Binding Proteins ; Drosophila Proteins ; Homeodomain Proteins ; Kr protein, Drosophila ; Kruppel-Like Transcription Factors ; Nerve Tissue Proteins ; POU Domain Factors ; Transcription Factors ; cas protein, Drosophila ; nub protein, Drosophila
Language	English
Publishing date	2012-02-25
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	808167-0
ISSN	1097-4199 ; 0896-6273
ISSN (online)	1097-4199
ISSN	0896-6273
DOI	10.1016/j.neuron.2011.12.018
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Zs.A 2496: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 92: Show issues

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