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Article ; Online: Antibacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release.

Schweizer, Tiziano A / Hertegonne, Sanne / Vulin, Clément / Zinkernagel, Annelies S / Mairpady Shambat, Srikanth

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2674, Page(s) 235–250

Abstract: Regulated cell death (RCD) and the concomitant release of extracellular traps by neutrophils (NETs) constitute an important antibacterial effector response. Usually, the dynamic processes of RCD and NETs release are assessed independently of each other ... ...

Abstract	Regulated cell death (RCD) and the concomitant release of extracellular traps by neutrophils (NETs) constitute an important antibacterial effector response. Usually, the dynamic processes of RCD and NETs release are assessed independently of each other by either unspecific or time-consuming methods. Here, we describe a flow cytometry-based high-throughput analysis method incorporating neutrophil RCD and NETs release with visual live-imaging conformation upon ex vivo bacterial challenge. This combined approach allows to quantify and closely follow the kinetics of the dynamic neutrophil effector response towards bacterial infection.
MeSH term(s)	Neutrophils/metabolism ; Extracellular Traps/metabolism ; Regulated Cell Death ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Flow Cytometry/methods
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-05-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3243-7_16
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Article ; Online: MEndoB, a chimeric lysin featuring a novel domain architecture and superior activity for the treatment of staphylococcal infections.

Roehrig, Christian / Huemer, Markus / Lorgé, Dominique / Arn, Fabienne / Heinrich, Nadine / Selvakumar, Lavanja / Gasser, Lynn / Hauswirth, Patrick / Chang, Chun-Chi / Schweizer, Tiziano A / Eichenseher, Fritz / Lehmann, Steffi / Zinkernagel, Annelies S / Schmelcher, Mathias

mBio

2024 Volume 15, Issue 2, Page(s) e0254023

Abstract: Bacterial infections are a growing global healthcare concern, as an estimated annual 4.95 million deaths are associated with antimicrobial resistance (AMR). Methicillin- ... ...

Abstract	Bacterial infections are a growing global healthcare concern, as an estimated annual 4.95 million deaths are associated with antimicrobial resistance (AMR). Methicillin-resistant
MeSH term(s)	Humans ; Animals ; Mice ; Staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus ; Peptidoglycan ; Zebrafish ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/microbiology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Staphylococcus ; N-Acetylmuramoyl-L-alanine Amidase/genetics ; N-Acetylmuramoyl-L-alanine Amidase/therapeutic use ; Sepsis/drug therapy
Chemical Substances	Peptidoglycan ; Anti-Bacterial Agents ; N-Acetylmuramoyl-L-alanine Amidase (EC 3.5.1.28)
Language	English
Publishing date	2024-01-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.02540-23
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Article ; Online: In Vitro Assessment of Bacterial Adhesion and Biofilm Formation on Novel Bioactive, Biodegradable Electrospun Fiber Meshes Intended to Support Tendon Rupture Repair.

Miescher, Iris / Rieber, Julia / Schweizer, Tiziano A / Orlietti, Mariano / Tarnutzer, Andrea / Andreoni, Federica / Meier Buergisser, Gabriella / Giovanoli, Pietro / Calcagni, Maurizio / Snedeker, Jess G / Zinkernagel, Annelies S / Buschmann, Johanna

ACS applied materials & interfaces

2024 Volume 16, Issue 5, Page(s) 6348–6355

Abstract: The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant ... ...

Abstract	The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant materials is an additional problem as it might lead to severe infections and impaired recovery. To counteract adhesion formation, two novel implant materials were fabricated by electrospinning, namely, a random fiber mesh containing hyaluronic acid (HA) and poly(ethylene oxide) (PEO) in a ratio of 1:1 (HA/PEO 1:1) and 1:4 (HA/PEO 1:4), respectively. Electrospun DegraPol (DP) treated with silver nanoparticles (DP-Ag) was developed to counteract the bacterial attachment. The three novel materials were compared to the previously described DP and DP with incorporated insulin-like growth factor-1 (DP-IGF-1), two implant materials that were also designed to improve tendon repair. To test whether the materials are prone to bacterial adhesion and biofilm formation, we assessed 10 strains of
MeSH term(s)	Humans ; Bacterial Adhesion ; Silver/pharmacology ; Silver/chemistry ; Insulin-Like Growth Factor I/pharmacology ; Metal Nanoparticles/chemistry ; Tendon Injuries/surgery ; Anti-Bacterial Agents/pharmacology ; Biofilms ; Tendons
Chemical Substances	Silver (3M4G523W1G) ; Insulin-Like Growth Factor I (67763-96-6) ; Anti-Bacterial Agents
Language	English
Publishing date	2024-01-30
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c15710
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Article: Pro-angiogenic and antibacterial copper containing nanoparticles in PLGA/amorphous calcium phosphate bone nanocomposites.

Näf, Lukas / Miescher, Iris / Pfuderer, Lara / Schweizer, Tiziano A / Brunner, David / Dürig, Johannes / Gröninger, Olivier / Rieber, Julia / Meier-Buergisser, Gabriella / Spanaus, Katharina / Calcagni, Maurizio / Bosshard, Philipp P / Achermann, Yvonne / Stark, Wendelin J / Buschmann, Johanna

Heliyon

2024 Volume 10, Issue 5, Page(s) e27267

Abstract: Large bone defects after trauma demand for adequate bone substitutes. Bone void fillers should be antibacterial and pro-angiogenic. One viable option is the use of composite materials like the combination of PLGA and amorphous calcium phosphate (aCaP). ... ...

Abstract	Large bone defects after trauma demand for adequate bone substitutes. Bone void fillers should be antibacterial and pro-angiogenic. One viable option is the use of composite materials like the combination of PLGA and amorphous calcium phosphate (aCaP). Copper stimulates angiogenesis and has antibacterial qualities. Either copper oxide (CuO) nanoparticles (NPs) were therefore added to PLGA/aCaP/CuO in different concentrations (1, 5 and 10 w/w %) or copper-doped tricalcium phosphate NPs (TCP with 2% of copper) were electrospun into PLGA/CuTCP nanocomposites. Bi-layered nanocomposites of PLGA/aCaP with different copper NPs (CuO or TCP) and a second layer of pristine PLGA were fabricated. Two clinical bacterial isolates (
Language	English
Publishing date	2024-03-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e27267
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Article ; Online: Limited Adaptation of

Räz, Anna K / Andreoni, Federica / Boumasmoud, Mathilde / Bergada-Pijuan, Judith / Schweizer, Tiziano A / Mairpady Shambat, Srikanth / Hasse, Barbara / Zinkernagel, Annelies S / Brugger, Silvio D

Microbiology spectrum

2023 Volume 11, Issue 4, Page(s) e0259021

Abstract: Staphylococcus aureus carriage is a risk factor for invasive infections. Unique genetic elements favoring the transition from colonizing to invasive phenotype have not yet been identified, and phenotypic adaptation traits are understudied. We therefore ... ...

Abstract	Staphylococcus aureus carriage is a risk factor for invasive infections. Unique genetic elements favoring the transition from colonizing to invasive phenotype have not yet been identified, and phenotypic adaptation traits are understudied. We therefore assessed phenotypic and genotypic profiles of 11 S. aureus isolate pairs sampled from colonized patients simultaneously suffering from invasive S. aureus infections. Ten out of 11 isolate pairs displayed the same
MeSH term(s)	Humans ; Staphylococcus aureus ; Staphylococcal Infections/microbiology ; Adaptation, Physiological ; Nasal Cavity/microbiology ; Virulence
Language	English
Publishing date	2023-06-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02590-21
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Article ; Online: Intervertebral disc cell chondroptosis elicits neutrophil response in

Schweizer, Tiziano A / Andreoni, Federica / Acevedo, Claudio / Scheier, Thomas C / Heggli, Irina / Maggio, Ewerton Marques / Eberhard, Nadia / Brugger, Silvio D / Dudli, Stefan / Zinkernagel, Annelies S

Frontiers in immunology

2022 Volume 13, Page(s) 908211

Abstract: To understand the pathophysiology of spondylodiscitis due ... ...

Abstract	To understand the pathophysiology of spondylodiscitis due to
MeSH term(s)	Animals ; Cytokines/metabolism ; Discitis/metabolism ; Discitis/pathology ; Humans ; Intervertebral Disc/metabolism ; Neutrophils/metabolism ; Retrospective Studies ; Staphylococcal Infections/metabolism ; Staphylococcus aureus/metabolism ; Swine
Chemical Substances	Cytokines
Language	English
Publishing date	2022-07-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.908211
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Article ; Online: Polyester Vascular Graft Material and Risk for Intracavitary Thoracic Vascular Graft Infection

Schweizer, Tiziano A / Shambat, Srikanth Mairpady / Haunreiter, Vanina Dengler / Mestres, Carlos A / Weber, Alberto / Maisano, Francesco / Zinkernagel, Annelies S / Hasse, Barbara

Emerging infectious diseases

2021 Volume 26, Issue 10, Page(s) 2448–2452

Abstract: Prosthetic vascular graft infections of the thoracic aorta are rare but can be fatal. Our comparison of collagen- and gelatin-coated grafts showed that collagen-coated grafts were associated with increased biofilm formation and bacterial adherence in ... ...

Abstract	Prosthetic vascular graft infections of the thoracic aorta are rare but can be fatal. Our comparison of collagen- and gelatin-coated grafts showed that collagen-coated grafts were associated with increased biofilm formation and bacterial adherence in vitro and with higher rates of perioperative vascular graft infections in vivo.
MeSH term(s)	Blood Vessel Prosthesis/adverse effects ; Collagen ; Gelatin ; Humans ; Polyesters ; Prosthesis-Related Infections/epidemiology
Chemical Substances	Polyesters ; Gelatin (9000-70-8) ; Collagen (9007-34-5)
Language	English
Publishing date	2021-01-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2610.191711
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Zs.A 4778: Show issues

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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Enzymatic debridement with bromelain and development of bacteremia in burn injuries: A retrospective cohort study.

Deplazes, Barla C / Hofmaenner, Daniel A / Scheier, Thomas C / Epprecht, Jana / Mayer, Michelle / Schweizer, Tiziano A / Buehler, Philipp K / Frey, Pascal M / Brugger, Silvio D

Burns : journal of the International Society for Burn Injuries

2023 Volume 50, Issue 2, Page(s) 405–412

Abstract: Background: Debridement is crucial for effective wound management in patients with severe burn injuries, and bromelain, a proteolytic enzyme from pineapple stems, has emerged as a promising alternative for surgery. However, potential links of bromelain ... ...

Abstract	Background: Debridement is crucial for effective wound management in patients with severe burn injuries, and bromelain, a proteolytic enzyme from pineapple stems, has emerged as a promising alternative for surgery. However, potential links of bromelain use to fever and sepsis have raised some concerns. Given the uncertainty as to whether this was caused by infection or other inflammatory sources, we aimed to investigate if the use of topical bromelain was associated with bacteremia. Methods: This single-centre retrospective cohort study included critically ill adult patients with severe burn injuries hospitalised at the Burn Center of the University Hospital Zurich between January 2017 and December 2021. Data were collected from two in-hospital electronic medical records databases. Our primary outcome, the association between topical bromelain treatment and the development of bacteremia, was investigated using a competing risk regression model, taking into account the competing risk of death. As a secondary outcome, the relationship between bromelain treatment and overall ICU mortality was examined using a Cox proportional hazards model. Results: The study included 269 patients with a median age of 50 years and median burnt total body surface area of 19%. A first bacteremia occurred in 61 patients (23%) after a median time of 6 days. Bromelain treatment was given to 83 (31%) of patients, with 22 (27%) of these developing bacteremia. In the fully adjusted competing risk regression model, no evidence for an association between bromelain treatment and bacteremia was found (SHR 0.79, 95%CI 0.42-1.48, p = 0.47). During hospital stay, 40 (15%) of patients died. There was no significant difference in mortality between patients treated with bromelain and those who were not (HR 0.55, 95%CI 0.26-1.20, p = 0.14). Among the five multidrug-resistant (MDR) pathogens identified, three were found in patients with bromelain treatment. Conclusion: Our study did not confirm an association between topical bromelain and bacteremia in patients with severe burn injuries. This finding can inform evidence-based practices by addressing concerns about potential risks of bromelain use, contributing to the development of more effective and safe burn wound management strategies.
MeSH term(s)	Adult ; Humans ; Middle Aged ; Retrospective Studies ; Debridement ; Bromelains/therapeutic use ; Burns/complications ; Bacteremia/drug therapy
Chemical Substances	Bromelains (9001-00-7)
Language	English
Publishing date	2023-12-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	197308-3
ISSN	1879-1409 ; 0305-4179
ISSN (online)	1879-1409
ISSN	0305-4179
DOI	10.1016/j.burns.2023.12.005
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Zs.A 1250: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Establishment of a localized acute implant-associated Staphylococcus aureus bone infection model in sheep.

Klein, Karina / Schweizer, Tiziano A / Siwy, Katharina / Lechmann, Beat / Karol, Agnieszka / von Rechenberg, Brigitte / Achermann, Yvonne / Darwiche, Salim E

Pathogens and disease

2021 Volume 79, Issue 6

Abstract: Orthopedic implant-associated bacterial infections with Staphylococcus aureus constitute a major clinical problem, and large pre-clinical animal models remain scarce. The aim of this study was to establish a standardized method of a localized, acute S. ... ...

Abstract	Orthopedic implant-associated bacterial infections with Staphylococcus aureus constitute a major clinical problem, and large pre-clinical animal models remain scarce. The aim of this study was to establish a standardized method of a localized, acute S. aureus bone infection in the presence of complex implanted devices in a sheep model. Four sheep underwent surgery receiving a complex implanted metallic device with a component stabilizing a bone defect created in the left tibial metaphysis, and an attached component placed in adjacent soft tissue. The bone defect was inoculated with S. aureus strain ATCC25293 (1 × 104 CFU). Twenty one days later, the surgery site was macroscopically evaluated, tissue samples and implants harvested for bacterial cell count quantification and tissue samples histologically analyzed. The animals exhibited clinical signs of localized infection (e.g. swelling, lameness, pain) but did not develop symptoms of sepsis. After euthanasia, macroscopic assessment revealed a localized bone and soft tissue infection at the surgery site. Histologically, an acute inflammation with neutrophils but also signs of bone destruction with necrosis was noted. An ovine model of a localized, acute S. aureus bone infection with complex implants was successfully established and could be used to test novel treatments against orthopedic implant-associated infections.
MeSH term(s)	Animals ; Biofilms/growth & development ; Disease Models, Animal ; Humans ; Osteomyelitis/diagnostic imaging ; Osteomyelitis/microbiology ; Osteomyelitis/pathology ; Prostheses and Implants/microbiology ; Prosthesis-Related Infections/microbiology ; Sheep ; Staphylococcal Infections/diagnostic imaging ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/pathology ; Staphylococcus aureus
Language	English
Publishing date	2021-01-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2049-632X
ISSN (online)	2049-632X
DOI	10.1093/femspd/ftab032
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Article ; Online: Genomic Surveillance of Vancomycin-Resistant Enterococcus faecium Reveals Spread of a Linear Plasmid Conferring a Nutrient Utilization Advantage.

Boumasmoud, Mathilde / Dengler Haunreiter, Vanina / Schweizer, Tiziano A / Meyer, Lilly / Chakrakodi, Bhavya / Schreiber, Peter W / Seidl, Kati / Kühnert, Denise / Kouyos, Roger D / Zinkernagel, Annelies S

mBio

2022 Volume 13, Issue 2, Page(s) e0377121

Abstract: Healthcare-associated outbreaks of vancomycin-resistant Enterococcus faecium (VREfm) are a worldwide problem with increasing prevalence. The genomic plasticity of this hospital-adapted pathogen contributes to its efficient spread despite infection ... ...

Abstract	Healthcare-associated outbreaks of vancomycin-resistant Enterococcus faecium (VREfm) are a worldwide problem with increasing prevalence. The genomic plasticity of this hospital-adapted pathogen contributes to its efficient spread despite infection control measures. Here, we aimed to identify the genomic and phenotypic determinants of health care-associated transmission of VREfm. We assessed the VREfm transmission networks at the tertiary-care University Hospital of Zurich (USZ) between October 2014 and February 2018 and investigated microevolutionary dynamics of this pathogen. We performed whole-genome sequencing for the 69 VREfm isolates collected during this time frame and assessed the population structure and variability of the vancomycin resistance transposon. Phylogenomic analysis allowed us to reconstruct transmission networks and to unveil external or wider transmission networks undetectable by routine surveillance. Notably, it unveiled a
MeSH term(s)	Enterococcus faecium/genetics ; Genomics ; Gram-Positive Bacterial Infections/microbiology ; Humans ; Nutrients ; Plasmids/genetics ; Vancomycin/pharmacology ; Vancomycin-Resistant Enterococci/genetics
Chemical Substances	Vancomycin (6Q205EH1VU)
Language	English
Publishing date	2022-03-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.03771-21
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