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Article ; Online: Cordyceps militaris polysaccharides: preparation and topical product application.

Kanlayavattanakul, Mayuree / Lourith, Nattaya

2023 Volume 10, Issue 1, Page(s) 3

Abstract: Background: Topical product derived from the fungus Cordyceps militaris was explored as a feasible method for an industrial practice.: Results: The mycelium residue of C. militaris that was industrial biotechnological produced was extracted with ... ...

Abstract	Background: Topical product derived from the fungus Cordyceps militaris was explored as a feasible method for an industrial practice. Results: The mycelium residue of C. militaris that was industrial biotechnological produced was extracted with water at different time conditions under ambient temperature, filtered and lyophilized. The extracts were all light to dark brown powder. The 24 h extraction was significantly (p < 0.01) highest in an extractive yield and total polysaccharides content (TPC) (43.33 ± 0.99% and 144.02 ± 2.06 mg glucose/g crude extract). This extract was proved to be stable following an accelerated stability test with the insignificant (p > 0.05) reduction of TPC (4.95 ± 2.23%). Topical product containing the extract were developed. Skin care preparation containing 0.2% extract was exhibited as the appropriated amount giving the stable cream. The developed C. militaris polysaccharide cream was confirmed safe and gained more than 70% of the overall preferences examined in 20 female volunteers. Conclusions: Cordyceps militaris mycelium residue is a beneficial source for pharmaceutical products. The C. militaris polysaccharides extract was prepared and qualified in terms of active content and stability. The extract was shown to be compatible with the available cosmetic ingredients. The safe and preferred C. militaris polysaccharides skin care cosmetics was developed. Accordingly, C. militaris polysaccharides skin care cosmetics that meets all the quality characters which are stable, safe, usable and efficient.
Language	English
Publishing date	2023-01-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2806612-1
ISSN	2054-3085 ; 2054-3085
ISSN (online)	2054-3085
ISSN	2054-3085
DOI	10.1186/s40694-023-00150-5
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Article ; Online: Wound healing and photoprotection properties of Acanthus ebracteatus Vahl. extracts standardized in verbascoside.

Kanlayavattanakul, Mayuree / Khongkow, Mattaka / Lourith, Nattaya

Scientific reports

2024 Volume 14, Issue 1, Page(s) 1904

Abstract: Acanthus spp. have been documented in traditional Thai herbal medicine and are applicable for the treatment of inflamed skin with wound healing property. Nonetheless, the scientific evidence necessary to prove the herb's doctrine has not yet been ... ...

Abstract	Acanthus spp. have been documented in traditional Thai herbal medicine and are applicable for the treatment of inflamed skin with wound healing property. Nonetheless, the scientific evidence necessary to prove the herb's doctrine has not yet been revealed. Verbascoside-rich extracts of the herbal medicine A. ebracteatus Vahl., were therefore prepared. The extracts and verbascoside were examined for their wound healing abilities using a scratch assay with fibroblasts. The anti-inflammatory effect suppressing MMP-9 was assessed in cocultures of keratinocyte (HaCaT cells) and fibroblasts. The extracts significantly improved wound healing compared with the control (p < 0.001). The wound healing effect of the extracts significantly (p < 0.01) increased with increasing verbascoside content. It should be noted that the extract was significantly (p < 0.05) better than verbascoside at the same test concentration. The extracts were capable of protecting cocultures of HaCaT cells and fibroblasts from photodamage. The extracts significantly (p < 0.001) suppressed cellular MMP-9 secretion following UV exposure, showing a better effect than that of verbascoside (p < 0.01). A. ebracteatus extract is promising for wound healing and photoprotection, and a prominent source of verbascoside. Verbascoside-rich A. ebracteatus could be utilized for the development of innovative skin-care products.
MeSH term(s)	Matrix Metalloproteinase 9 ; Wound Healing ; Acanthaceae ; Phenols/pharmacology ; Plant Extracts/pharmacology ; Glucosides ; Polyphenols
Chemical Substances	acteoside (3TGX09BD5B) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Phenols ; Plant Extracts ; Glucosides ; Polyphenols
Language	English
Publishing date	2024-01-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52511-8
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Article: Moringa leaf: An innovative source of antioxidative phenolics for cosmeceutical products

Kanlayavattanakul, Mayuree / Lourith, Nattaya

Scientia horticulturae. 2022 Mar. 15, v. 295

2022

Abstract: Moringa oleifera Lam. (Moringaceae) or Moringa or miracle tree has been utilized in several forms in food and nutraceuticals. However, preparation of the antioxidative, phenolic-rich fraction of Moringa leaves is sparsely reported as per its status as an ...

Abstract	Moringa oleifera Lam. (Moringaceae) or Moringa or miracle tree has been utilized in several forms in food and nutraceuticals. However, preparation of the antioxidative, phenolic-rich fraction of Moringa leaves is sparsely reported as per its status as an innovative product. A concise and processable extraction yielding phenolic-rich fractions derived from Moringa leaves was developed. Maceration of mature Moringa leaves in 70% ethanolic water for 24 h at room temperature afforded a significant phenolic extract with antioxidant activity (10.92 ± 0.23 g GAE/100 g extract and IC₅₀ = 50.22 ± 0.09 µg/ml) significantly greater (p < 0.05) than those resulting from other methods. The extract was applicable for topical products in terms of its physicochemical properties and compatibility, resulting in a stable water-in-silicone (W/Si) emulsion. This antioxidative, phenolic-rich Moringa emulsion can be used as a cosmeceutical product. The innovative Moringa product presented is meeting the consumers’ preferences for natural/sustainable products that are continuing to increase year by year. In addition, successive integration between the agricultural crop and cosmetic industries are evidenced and significance to horticulture.
Keywords	Moringa oleifera ; ambient temperature ; antioxidant activity ; cosmetics ; crops ; dietary supplements ; emulsions ; horticulture ; leaves ; maceration ; phenolic compounds ; trees
Language	English
Dates of publication	2022-0315
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2022.110894
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Sacha Inchi: The Promising Source of Functional Oil for Anti-Aging Product.

Lourith, Nattaya / Kanlayavattanakul, Mayuree / Chaikul, Puxvadee

Journal of oleo science

2023 Volume 73, Issue 4, Page(s) 429–435

Abstract: Sacha inchi (Plukenetia volubilis) oil is constituted with macronutrients and the health benefit fatty acids. In this context, the efficient of Sacha inchi oil for anti-aging product is presented. The light-clear yellowish seed oil of Sacha inchi was ... ...

Abstract	Sacha inchi (Plukenetia volubilis) oil is constituted with macronutrients and the health benefit fatty acids. In this context, the efficient of Sacha inchi oil for anti-aging product is presented. The light-clear yellowish seed oil of Sacha inchi was revealed on its physicochemical properties that are in the same range of the commercializing plant-oil supplied for topical products. The oil was GC/MS exhibited to be constituted with α-linolenic (51.72%) and linoleic (24.3%) acids, with unsaturated/saturated fatty acids ratio of 21.26. The oil was noted onto its potent in vitro antioxidant activity assessed by ABTS, DPPH and FRAP assays. In addition, the oil (1-3%) was proved to be safe in normal human fibroblast cells. Furthermore, the oil exhibited cellular antioxidant with inhibitory effect against MMP-2. Sacha inchi oil is therefore highlighted as a potential source of nutraceutical especially for anti-aging product. The oil is specified for the product development in terms of physicochemical, chemical and biological profiles. Innovative processing of Sacha inchi is therefore encouraged as the promising plant for anti-aging product.
MeSH term(s)	Humans ; Fatty Acids, Unsaturated ; Fatty Acids ; Plant Oils/pharmacology ; Plant Oils/chemistry ; Aging ; Antioxidants/pharmacology ; Euphorbiaceae/chemistry
Chemical Substances	Fatty Acids, Unsaturated ; Fatty Acids ; Plant Oils ; Antioxidants
Language	English
Publishing date	2023-12-29
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2218264-0
ISSN	1347-3352 ; 1345-8957
ISSN (online)	1347-3352
ISSN	1345-8957
DOI	10.5650/jos.ess23147
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Article ; Online: Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing.

Kanlayavattanakul, Mayuree / Khongkow, Mattaka / Klinngam, Wannita / Chaikul, Puxvadee / Lourith, Nattaya / Chueamchaitrakun, Piyaporn

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2253

Abstract: Tea (Camellia spp.) is an important medicinal herb. C. sinensis var. sinensis is the most studied tea variety due to its more preferred flavor than C. sinensis var. assamica (Assam tea), the less economic importance with more bitter variety. A bitter ... ...

Abstract	Tea (Camellia spp.) is an important medicinal herb. C. sinensis var. sinensis is the most studied tea variety due to its more preferred flavor than C. sinensis var. assamica (Assam tea), the less economic importance with more bitter variety. A bitter taste highlights its potential as a candidate source for tea catechins, the health beneficial actives applicable for ageing treatment. Nonetheless, indicative data for tea on UV-induced and senescent ageing remain unclarified. Assam tea extract (ATE) was prepared and standardized in terms of TPC, TFC and TTC. EGCG was HPLC quantified as the prime ATE catechin. In vitro antioxidant activity of ATE was exhibited with ABTS, DPPH and FRAP assays. ATE's cellular antioxidant activity was indicated in HDFs at a stronger degree than ascorbic acid. The photoaging protection of ATE was evidenced in a coculture of HaCaT cells and HDFs. ATE markedly suppressed UV-induced IL-6, IL-8, MMP-1 and MMP-9 expressions. The proficiency of ATE targeting on senescent ageing was demonstrated in an ex vivo human skin model, where IL-6 and MMP-1 expressions were suppressed, whilst hyaluronic acid and collagen syntheses were promoted. ATE was chemically stabled as indicated by the catechin contents and color parameters following 6 months storage under conditions recommended for topical product. ATE enriched in catechins warrants its applicability as a new generation of photoaging protectant agent promising for the prevention and treatment for senescent ageing. The findings indicate the proficiency of ATE for innovative anti-ageing agent.
MeSH term(s)	Humans ; Tea/chemistry ; Camellia sinensis/chemistry ; Catechin/pharmacology ; Catechin/chemistry ; Antioxidants/pharmacology ; Antioxidants/analysis ; Matrix Metalloproteinase 1 ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Skin Aging ; Interleukin-6 ; Aging
Chemical Substances	Tea ; Catechin (8R1V1STN48) ; Antioxidants ; Matrix Metalloproteinase 1 (EC 3.4.24.7) ; Plant Extracts ; Interleukin-6
Language	English
Publishing date	2024-01-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52781-2
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Article ; Online: Anti-skin ageing activities of rice (Oryza sativa) bran soft and hard waxes in cultured skin cells.

Chaikul, Puxvadee / Kanlayavattanakul, Mayuree / Khongkow, Mattaka / Jantimaporn, Angkana / Lourith, Nattaya

International journal of cosmetic science

2023 Volume 46, Issue 2, Page(s) 162–174

Abstract: Objective: Rice (Oryza sativa) bran waxes, the by-products of rice bran oil manufacturing, are widely used as inactive components in several preparations. Nevertheless, the function of rice bran waxes against skin ageing has never been reported. This ... ...

Abstract	Objective: Rice (Oryza sativa) bran waxes, the by-products of rice bran oil manufacturing, are widely used as inactive components in several preparations. Nevertheless, the function of rice bran waxes against skin ageing has never been reported. This study aimed to investigate thermal property and fatty acid profile of rice bran waxes, including rice bran soft (RBS) and hard (RBH) waxes, and the activities against skin ageing in cultured skin cells. Methods: Thermal property and fatty acid profile of rice bran waxes were analysed by differential scanning calorimetry and gas chromatography-mass spectrometry, respectively. The cytotoxicity assay of waxes was performed in B16F10 melanoma cells, human skin fibroblasts and co-culture cells of HaCaT cells and human skin fibroblasts. The non-cytotoxic concentrations of waxes were evaluated for their activities against skin ageing, including melanogenesis assay, antioxidant activity, collagen content analysis, matrix metalloproteinase-1 and matrix metalloproteinase-2 inhibitory assay and anti-inflammatory activity. Results: Thermal property indicated the endotherm peaks with melting temperatures at 40.89 ± 0.27°C and 69.64 ± 0.34°C for RBS and RBH, respectively. The main fatty acids in RBS were oleic (31.68 ± 0.75%) and linoleic acids (27.19 ± 0.40%), whereas those in RBH were palmitic (36.24 ± 1.08%) and stearic acids (35.21 ± 4.51%). The cytotoxicity assay in single cells and co-culture cells showed the non-cytotoxicity of RBS (0.0001-1 mg/mL) and RBH (0.0001-0.1 mg/mL). The anti-skin ageing activities of 1 mg/mL RBS and 0.1 mg/mL RBH included the melanogenesis inhibition by suppression of tyrosinase and tyrosinase-related protein-2 enzymes, the antioxidant activity by cellular protection against cell damage and cell death, the collagen stimulation, the matrix metalloproteinase-1 and matrix metalloproteinase-2 suppression and the anti-inflammation. Conclusions: The study results suggest that RBS and RBH can potentially be applied as the functional ingredients in formulations against skin ageing as well as provide the superior benefit on skin moisturization.
MeSH term(s)	Humans ; Waxes/chemistry ; Matrix Metalloproteinase 2 ; Antioxidants/pharmacology ; Oryza/chemistry ; Matrix Metalloproteinase 1 ; Skin Aging ; Fatty Acids ; Collagen
Chemical Substances	Waxes ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Antioxidants ; Matrix Metalloproteinase 1 (EC 3.4.24.7) ; Fatty Acids ; Collagen (9007-34-5)
Language	English
Publishing date	2023-12-20
Publishing country	England
Document type	Journal Article
ZDB-ID	198917-0
ISSN	1468-2494 ; 0142-5463
ISSN (online)	1468-2494
ISSN	0142-5463
DOI	10.1111/ics.12918
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Article: Formulation and clinical evaluation of the standardized Litchi chinensis extract for skin hyperpigmentation and aging treatments.

Lourith, N / Kanlayavattanakul, M

Annales pharmaceutiques francaises

2020 Volume 78, Issue 2, Page(s) 142–149

Abstract: Introduction: The standardized litchi extract had been revealed on phytochemical actives, in vitro and cellular activities against aging and darkening of skin. However, a formulation containing the extract has never been developed as per clinical ... ...

Abstract	Introduction: The standardized litchi extract had been revealed on phytochemical actives, in vitro and cellular activities against aging and darkening of skin. However, a formulation containing the extract has never been developed as per clinical evaluated. Materials and methods: The litchi serum was developed, safety and efficacy were clinically evaluated in human volunteers. The stable and none irritated 0.05 and 0.1% litchi serums were randomized-single blind placebo control clinical applied on the inner forearm of 29 volunteers for a consecutive 112 days and monitored by Mexameter® MX18, Cutometer® MPA 580 and Visioscan® VC 98. Results: Skin lightening efficacy of the 0.1% and 0.05% litchi serum was significantly (P<0.001 and P<0.05) higher than the placebo. Skin elasticity and wrinkle reduction was significantly (P<0.05 and P<0.005) achieved by the 0.1% litchi serum. The efficacy of litchi serums was confirmed by a split-face, randomized, single-blind controlled that the 0.1% litchi serum was significantly (P<0.05) better than the 0.05% one of all examined parameters. Conclusion: Safety and efficacy of litchi extract are clinically confirmed for hyperpigmentation and aging of skin treatments.
Language	English
Publishing date	2020-01-23
Publishing country	France
Document type	Journal Article
ZDB-ID	307-4
ISSN	0003-4509
ISSN	0003-4509
DOI	10.1016/j.pharma.2020.01.004
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Article: Improved Stability of Butterfly Pea Anthocyanins with Biopolymeric Walls.

Lourith, Nattaya / Kanlayavattanakul, Mayuree

Journal of cosmetic science

2020 Volume 71, Issue 1, Page(s) 1–10

Abstract: Butterfly pea ( ...

Abstract	Butterfly pea (
MeSH term(s)	Anthocyanins ; Color ; Gum Arabic ; Pisum sativum ; Plant Extracts
Chemical Substances	Anthocyanins ; Plant Extracts ; Gum Arabic (9000-01-5)
Language	English
Publishing date	2020-04-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	1435667-3
ISSN	1525-7886 ; 0037-9832
ISSN	1525-7886 ; 0037-9832
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Article: Phyllanthus emblica

Chaikul, Puxvadee / Kanlayavattanakul, Mayuree / Somkumnerd, Jariya / Lourith, Nattaya

Journal of traditional and complementary medicine

2021 Volume 11, Issue 5, Page(s) 390–399

Abstract: Background and aim: Skin aging influences the changes in skin, including skin dryness, wrinkle, and irregular pigmentation. Amla (: Experimental procedure: Amla branches were standardized the phenolic acids. The extract was investigated anti-skin ... ...

Abstract	Background and aim: Skin aging influences the changes in skin, including skin dryness, wrinkle, and irregular pigmentation. Amla ( Experimental procedure: Amla branches were standardized the phenolic acids. The extract was investigated anti-skin aging activities, including antioxidant, anti-tyrosinase, anti-melanogenesis, and matrix metalloproteinase-2 inhibitory assays. Topical gel containing extract was prepared and evaluated the skin irritation by a single closed patch test. Randomized, double-blind, placebo-control study was performed in 20 volunteers for 84 consecutive days. The tested skin was evaluated by Chromameter® CR 400, Dermalab® USB, Mexameter® MX 18, Corneometer® CM 825, and Visioscan® VC 98. Results: Amla branch extract, a dark brown powder, consisted a variety of phenolic acids, mainly sinapic and ferulic acids. The extract exhibited the potent antioxidant and tyrosinase inhibitory activities Conclusion: The study results suggested that amla branch is a rich source of bioactive compounds and can be a potential ingredient for utilization in anti-skin aging products.
Language	English
Publishing date	2021-02-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2709698-1
ISSN	2225-4110
ISSN	2225-4110
DOI	10.1016/j.jtcme.2021.02.004
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Article: Valorization of spent coffee grounds as the specialty material for dullness and aging of skin treatments

Kanlayavattanakul, Mayuree / Lourith, Nattaya / Chaikul, Puxvadee

Chemical and biological technologies in agriculture. 2021 Dec., v. 8, no. 1

2021

Abstract: BACKGROUND: Coffee beans contain oil with health benefits from fatty acids. The unprocessed and processed coffee beans are mostly identical in coffee oil quality and are substantively supplied for certain industries. However, the cost-effective ... ...

Abstract	BACKGROUND: Coffee beans contain oil with health benefits from fatty acids. The unprocessed and processed coffee beans are mostly identical in coffee oil quality and are substantively supplied for certain industries. However, the cost-effective valorization of specialty ingredients from spent coffee grounds for cosmetics is sparely presented. Linoleic acid-rich spent coffee oil, as a specialty material for skin lightening and antiaging cosmetics, is objectively to be presented. RESULTS: Spent coffee oils were prepared by different methods. The most cost-effective material with a high extraction yield, linoleic acid content and unsaturated/saturated fatty acid (UFA/SFA) ratio (13.21 ± 0.25, 32.09% and 0.97) was modified. The modified oil was boosted in linoleic acid (77.20% or 140.57% improvement) and the UFA/SFA ratio (33.12). The physicochemical properties of the oil were applicable for cosmetics as per its safety profiles in B16F10 melanoma and normal human skin fibroblast cells. The oil significantly better inhibited cellular melanogenesis than kojic and linoleic acids (p < 0.01), with prominent tyrosinase and TRP-2 inhibitions. The cellular antioxidant activity of the oil was comparable to those of ascorbic and linoleic acids. The collagen stimulating efficacy of the oil was significantly better than that of ascorbic but comparable to that of linoleic acid as indicated by the MMP-2 inhibitory activities (p < 0.01 and p < 0.001, respectively). CONCLUSIONS: The oil is a specialty material for skin brightening and skin wrinkle reduction/skin elasticity improvement products. A successive circular bioeconomy of spent coffee ground waste in a more profitable cosmetic industry is indicated.
Keywords	agriculture ; antioxidant activity ; bioeconomics ; collagen ; cosmetics ; cost effectiveness ; fibroblasts ; industry ; linoleic acid ; melanogenesis ; melanoma ; oils ; skin (animal) ; wastes
Language	English
Dates of publication	2021-12
Size	p. 55.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	2762782-2
ISSN	2196-5641
ISSN	2196-5641
DOI	10.1186/s40538-021-00252-5
Database	NAL-Catalogue (AGRICOLA)

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