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Article ; Online: Mechanic study based on untargeted metabolomics of Pi-pa-run-fei-tang on pepper combined with ammonia induced chronic cough model mice.

Jie, Xiao-Lu / Tong, Zhe-Ren / Xu, Xin-Yue / Wu, Jia-Hui / Jiang, Xing-Liang / Tao, Yi / Feng, Pei-Shi / Yu, Jin / Lan, Ji-Ping / Wang, Ping

Journal of ethnopharmacology

2024 Volume 326, Page(s) 117905

Abstract: Ethnopharmacological relevance: Pi-pa-run-fei-tang (PPRFT), a traditional Chinese medicine formula ...

Abstract	Ethnopharmacological relevance: Pi-pa-run-fei-tang (PPRFT), a traditional Chinese medicine formula with long-standing history, demonstrated beneficial effect on chronic cough. However, the mechanism underlying efficacy unclear. In current research, we explored the impact and molecular mechanism of chronic cough mouse stimulating with capsaicin combined with ammonia. Aim of the study: To investigate the metabolic modulating effects, and potential mechanisms underlying the therapeutic effect of PPRFT in chronic cough. Materials and methods: Chronic cough mouse models were created by stimulating mice by capsaicin combined with ammonia. Number of coughs and cough latency within 2 min were recorded. With lung tissue and serum samples collected for histopathology, metabolomics, RT-qPCR, immunohistochemistry, and WB analysis. Lymphocytes were isolated and flow cytometric assays were conducted to evaluate the differentiation between Th17 and Treg cell among CD4 Results: Results indicated that PPRFT obviously reduced the number of coughs, prolonged cough latency, reduced inflammatory cell infiltration and lung tissues damage, and decreased the serum level of IL-6, IL-1β, TNF-α, and IL-17 while increasing IL-10 levels. Notably, PPRFT suppressed Th17 cell divergence and promoted Treg cell divergence. Furthermore, serum metabolomic assays showed that 46 metabolites differed significantly between group, with 35 pathways involved. Moreover, mRNA levels of IL-6, NF-κB, IL-17, RORγT, JAK2, STAT3, PI3K and AKT in lung tissues remarkably reduced and mRNA levels of IL-10 and FOXP3 were elevated after PPRFT pretreatment. Additionally, PPRFT treatments decreased the protein levels of IL-6, NF-κB, IL-17, RORγT, p-JAK2, p-STAT3, p-PI3K, and p-AKT and increased the protein levels of IL-10 and FOXP3, but no significantly effects to the levels on JAK2, STAT3, PI3K, and AKT in the lungs. Conclusion: Conclusively, our result suggested the effect with PPRFT on chronic cough may be mediated through IL-6/JAK2/STAT3 and PI3K/AKT/NF-κB pathway, which regulate the differentiation between Th17 and Treg cell. This beneficial effect of PPRFT in capsaicin and ammonia-stimulated chronic cough mice indicates its potential application in treating chronic cough.
MeSH term(s)	Mice ; Animals ; Interleukin-10/metabolism ; Cytokines/metabolism ; Interleukin-17/metabolism ; NF-kappa B/metabolism ; Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism ; Ammonia/metabolism ; Interleukin-6/metabolism ; Chronic Cough ; Capsaicin/pharmacology ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; T-Lymphocytes, Regulatory ; Forkhead Transcription Factors/metabolism ; RNA, Messenger/metabolism ; Th17 Cells
Chemical Substances	Interleukin-10 (130068-27-8) ; Cytokines ; Interleukin-17 ; NF-kappa B ; Nuclear Receptor Subfamily 1, Group F, Member 3 ; Ammonia (7664-41-7) ; Interleukin-6 ; Capsaicin (S07O44R1ZM) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Forkhead Transcription Factors ; RNA, Messenger
Language	English
Publishing date	2024-02-15
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.117905
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Article ; Online: Nonvolatile Electrical Control and Reversible Gas Capture by Ferroelectric Polarization Switching in 2D FeI

Liu, Guogang / Chen, Tong / Zhou, Guanghui / Xu, Zhonghui / Xiao, Xianbo

ACS sensors

2023 Volume 8, Issue 4, Page(s) 1440–1449

Abstract: ... heterostructures consisting of a ferromagnetic FeI ...

Abstract	Nonvolatile electrical control is the core of future magnetoelectric nanodevices. In this work, we systematically explore both the electronic structures and transport properties of multiferroic van der Waals (vdW) heterostructures consisting of a ferromagnetic FeI
MeSH term(s)	Adsorption ; Electricity ; Electronics ; Magnets
Language	English
Publishing date	2023-03-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-3694
ISSN (online)	2379-3694
DOI	10.1021/acssensors.2c02365
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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma.

Jie, Xiao-Lu / Luo, Zi-Rui / Yu, Jin / Tong, Zhe-Ren / Li, Qiao-Qiao / Wu, Jia-Hui / Tao, Yi / Feng, Pei-Shi / Lan, Ji-Ping / Wang, Ping

Journal of ethnopharmacology

2023 Volume 317, Page(s) 116719

Abstract: Ethnopharmacological relevance: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription ...

Abstract	Ethnopharmacological relevance: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma injury by affecting host metabolism. Untargeted metabolomics can be used to better understand the biological mechanisms underlying asthma development and identify early biomarkers that can help advance treatment. Aim of the study: The aim of this study was to verification the efficacy of PPRFT in the treatment of asthma and to preliminarily explore its mechanism. Materials and methods: A mouse asthma model was built by OVA induction. Inflammatory cell in BALF was counted. The level of IL-6, IL-1β, and TNF-α in BALF were measured. The levels of IgE in the serum and EPO, NO, SOD, GSH-Px, and MDA in the lung tissue were measured. Furthermore, pathological damage to the lung tissues was detected to evaluate the protective effects of PPRFT. The serum metabolomic profiles of PPRFT in asthmatic mice were determined by GC-MS. The regulatory effects on mechanism pathways of PPRFT in asthmatic mice were explored via immunohistochemical staining and western blotting analysis. Results: PPRFT displayed lung-protective effects through decreasing oxidative stress, airway inflammation, and lung tissue damage in OVA-induced mice, which was demonstrated by decreasing inflammatory cell levels, IL-6, IL-1β, and TNF-α levels in BALF, and IgE levels in serum, decreasing EPO, NO, and MDA levels in lung tissue, elevating SOD and GSH-Px levels in lung tissue and lung histopathological changes. In addition, PPRFT could regulate the imbalance in Th17/Treg cell ratios, suppress RORγt, and increase the expression of IL-10 and Foxp3 in the lung. Moreover, PPRFT treatment led to decreased expression of IL-6, p-JAK2/Jak2, p-STAT3/STAT3, IL-17, NF-κB, p-AKT/AKT, and p-PI3K/PI3K. Serum metabolomics analysis revealed that 35 metabolites were significantly different among different groups. Pathway enrichment analysis indicated that 31 pathways were involved. Moreover, correlation analysis and metabolic pathway analysis identified three key metabolic pathways: galactose metabolism; tricarboxylic acid cycle; and glycine, serine, and threonine metabolism. Conclusion: This research indicated that PPRFT treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating serum metabolism. The anti-asthmatic activity of PPRFT may be associated with the regulatory effects of IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB mechanistic pathways.
MeSH term(s)	Mice ; Animals ; NF-kappa B/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Ovalbumin/toxicity ; Interleukin-6/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Lung Injury/chemically induced ; Lung Injury/drug therapy ; Lung Injury/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Interleukin-17/metabolism ; T-Lymphocytes, Regulatory ; Disease Models, Animal ; Cytokines/metabolism ; Asthma/chemically induced ; Asthma/drug therapy ; Asthma/metabolism ; Signal Transduction ; Lung ; Immunoglobulin E ; Superoxide Dismutase/metabolism ; Mice, Inbred BALB C
Chemical Substances	NF-kappa B ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Ovalbumin (9006-59-1) ; Interleukin-6 ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Tumor Necrosis Factor-alpha ; Interleukin-17 ; Cytokines ; Immunoglobulin E (37341-29-0) ; Superoxide Dismutase (EC 1.15.1.1)
Language	English
Publishing date	2023-05-31
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116719
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Zs.A 1494: Show issues

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Article ; Online: Bu-Fei decoction and modified Bu-Fei decoction inhibit the growth of non-small cell lung cancer, possibly via inhibition of apurinic/apyrimidinic endonuclease 1.

Jiang, Shan-Tong / Han, Shu-Yan / Pang, Li-Na / Jiao, Yan-Na / He, Xi-Ran / Li, Ping-Ping

International journal of molecular medicine

2018 Volume 41, Issue 4, Page(s) 2128–2138

Abstract: ... Fei decoction (BFD) is a traditional Chinese herbal formula, which is believed to supplement Qi, clear ... away heat and nourish the lungs. BFD and modified Bu‑Fei decoction (MBFD) have been used in China ...

Abstract	Human apurinic/apyrimidinic endonuclease 1 (APE1) is a ubiquitous multifunctional protein, which possesses DNA repair and redox activities. High levels of APE1 are associated with chemo‑ and radioresistance, and poor prognosis in various types of cancer, including non‑small cell lung cancer (NSCLC). Bu‑Fei decoction (BFD) is a traditional Chinese herbal formula, which is believed to supplement Qi, clear away heat and nourish the lungs. BFD and modified Bu‑Fei decoction (MBFD) have been used in China to treat patients with lung cancer. The present study aimed to evaluate the potential antitumor effects of BFD and MBFD on NSCLC in vitro and in vivo. In addition, the possible contribution of APE1 was examined. MTT assay was used to investigated the anti-tumor activity of BFD and MBFD on H1975 and H292 NSCLC cell lines. The DNA damage of cells in the control and the experimental groups was detected using comet assay. The in vivo anti-tumor effects of BFD and MBFD were evaluated in a NSCLC tumor nude mouse xenograft model. Polymerase chain reaction (PCR), reverse transcription‑quantitative PCR (RT‑qPCR) analysis and western blot analysis were applied to analyze the mRNA and protein expression levels of APE1 in H1975 and H292 cells, so as to the xenograft tumor tissues. The concentration of APE1 in mice plasma was determined using enzyme linked immunosorbent assay (ELISA). In vitro, BFD and MBFD inhibited the growth of cultured H1975 and H292 NSCLC cells. The results of a comet assay revealed that BFD and MBFD increased DNA damage. Furthermore, the expression levels of APE1 were decreased in response to BFD and MBFD at the mRNA and protein levels. In mice carrying NSCLC xenografts, BFD and MBFD inhibited tumor growth and decreased APE1 expression. In addition, in normal human lung bronchial epithelial BEAS‑2B cells, the half maximal inhibitory concentrations of BFD and MBFD were much higher compared with in NSCLC cells, and they had no effect on DNA damage. These results suggested that BFD and MBFD may inhibit the growth of NSCLC, possibly by inhibiting APE1 expression.
MeSH term(s)	Animals ; Antineoplastic Agents, Phytogenic/pharmacology ; Antineoplastic Agents, Phytogenic/therapeutic use ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Cycle/drug effects ; Cell Line ; Cell Line, Tumor ; Cell Proliferation/drug effects ; DNA Repair/drug effects ; DNA-(Apurinic or Apyrimidinic Site) Lyase/antagonists & inhibitors ; DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics ; Down-Regulation/drug effects ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Lung Neoplasms/drug therapy ; Lung Neoplasms/genetics ; Lung Neoplasms/pathology ; Mice, Inbred BALB C ; Mice, Nude ; RNA, Messenger/antagonists & inhibitors ; RNA, Messenger/genetics
Chemical Substances	Antineoplastic Agents, Phytogenic ; Drugs, Chinese Herbal ; RNA, Messenger ; bu-fei decoction ; APEX1 protein, human (EC 4.2.99.18) ; DNA-(Apurinic or Apyrimidinic Site) Lyase (EC 4.2.99.18)
Language	English
Publishing date	2018-01-30
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1444428-8
ISSN	1791-244X ; 1107-3756
ISSN (online)	1791-244X
ISSN	1107-3756
DOI	10.3892/ijmm.2018.3444
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Zs.A 4942: Show issues

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Article: Effect of COD/N Ratio on Removal Performances in Two Subsurface Wastewater Infiltration Systems: Fei et al

Fei, Hexin / Pan, Jing / Tong, Deli / Huang, Linli / Yu, Long / Sun, Yafei / Qi, Shiyue / Huang, Yaoyao

Water environment research. 2017 Aug., v. 89, no. 8

2017

Abstract: Dissolved oxygen (DO), were investigated. Aerobic conditions were effectively developed in 50 cm depth of the matrix and anoxic or anaerobic conditions were not changed in 80 and 110 cm depth by intermittent aeration, which encouraged nitrification. ... ...

Abstract	Dissolved oxygen (DO), were investigated. Aerobic conditions were effectively developed in 50 cm depth of the matrix and anoxic or anaerobic conditions were not changed in 80 and 110 cm depth by intermittent aeration, which encouraged nitrification. Increased influent COD/N ratio led to lower COD and nitrogen removal in conventional SWISs. Sufficient carbon source in high COD/N ratio influent promoted denitrification with intermittent aeration. High removal rates of COD (95.68 ± 0.21%), TP (92.02 ± 0.28%), ‐N (99.33 ± 0.05%), and ‐ (89.65 ± 0.6%) were obtained with influent COD/N ratio of 12 in aerated SWISs. Under the COD/N ratio of 12 and 18, intermittent aeration boosted the growth and reproduction of nitrifying bacteria and denitrifying bacteria. Meanwhile, nitrate and nitrite reductase activities with intermittent aeration were higher than that without aeration in 80 and 110 cm depths.
Keywords	aeration ; carbon ; denitrification ; dissolved oxygen ; nitrates ; nitrification ; nitrite reductase ; nitrogen ; reproduction ; research ; wastewater ; water
Language	English
Dates of publication	2017-08
Size	p. 694-702.
Publishing place	Water Environment Federation
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1098976-6
ISSN	1554-7531 ; 1047-7624 ; 1061-4303
ISSN (online)	1554-7531
ISSN	1047-7624 ; 1061-4303
DOI	10.2175/106143017X14839994522867
Database	NAL-Catalogue (AGRICOLA)

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Z 4344: Show issues

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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma

Jie, Xiao-Lu / Luo, Zi-Rui / Yu, Jin / Tong, Zhe-Ren / Li, Qiao-Qiao / Wu, Jia-Hui / Tao, Yi / Feng, Pei-Shi / Lan, Ji-Ping / Wang, Ping

Journal of Ethnopharmacology. 2023 Dec., v. 317 p.116719-

2023

Abstract: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma ...

Abstract	Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma injury by affecting host metabolism. Untargeted metabolomics can be used to better understand the biological mechanisms underlying asthma development and identify early biomarkers that can help advance treatment. The aim of this study was to verification the efficacy of PPRFT in the treatment of asthma and to preliminarily explore its mechanism. A mouse asthma model was built by OVA induction. Inflammatory cell in BALF was counted. The level of IL-6, IL-1β, and TNF-α in BALF were measured. The levels of IgE in the serum and EPO, NO, SOD, GSH-Px, and MDA in the lung tissue were measured. Furthermore, pathological damage to the lung tissues was detected to evaluate the protective effects of PPRFT. The serum metabolomic profiles of PPRFT in asthmatic mice were determined by GC-MS. The regulatory effects on mechanism pathways of PPRFT in asthmatic mice were explored via immunohistochemical staining and western blotting analysis. PPRFT displayed lung-protective effects through decreasing oxidative stress, airway inflammation, and lung tissue damage in OVA-induced mice, which was demonstrated by decreasing inflammatory cell levels, IL-6, IL-1β, and TNF-α levels in BALF, and IgE levels in serum, decreasing EPO, NO, and MDA levels in lung tissue, elevating SOD and GSH-Px levels in lung tissue and lung histopathological changes. In addition, PPRFT could regulate the imbalance in Th17/Treg cell ratios, suppress RORγt, and increase the expression of IL-10 and Foxp3 in the lung. Moreover, PPRFT treatment led to decreased expression of IL-6, p-JAK2/Jak2, p-STAT3/STAT3, IL-17, NF-κB, p-AKT/AKT, and p-PI3K/PI3K. Serum metabolomics analysis revealed that 35 metabolites were significantly different among different groups. Pathway enrichment analysis indicated that 31 pathways were involved. Moreover, correlation analysis and metabolic pathway analysis identified three key metabolic pathways: galactose metabolism; tricarboxylic acid cycle; and glycine, serine, and threonine metabolism. This research indicated that PPRFT treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating serum metabolism. The anti-asthmatic activity of PPRFT may be associated with the regulatory effects of IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB mechanistic pathways.
Keywords	animal models ; asthma ; biomarkers ; blood serum ; galactose ; histopathology ; immunohistochemistry ; inflammation ; interleukin-10 ; interleukin-17 ; interleukin-6 ; lungs ; metabolism ; metabolites ; metabolomics ; mice ; oxidative stress ; serine ; threonine ; traditional medicine ; tricarboxylic acid cycle ; PPRFT ; Th17/Treg
Language	English
Dates of publication	2023-12
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116719
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo.

He, Xi-Ran / Han, Shu-Yan / Li, Xiao-Hong / Zheng, Wen-Xian / Pang, Li-Na / Jiang, Shan-Tong / Li, Ping-Ping

Journal of ethnopharmacology

2017 Volume 204, Page(s) 45–57

Abstract: Ethnopharmacological relevance: Traditional Chinese medicine Bu-Fei decoction (BFD) has been ...

Abstract	Ethnopharmacological relevance: Traditional Chinese medicine Bu-Fei decoction (BFD) has been utilized to treat patients with Qi deficiency for decades, with the advantages of invigorating vital energy, clearing heat-toxin and moistening lung, etc. According to previous clinical experience and trials, BFD has been found to indeed improve life quality of lung cancer patients and prolong survival time. Nevertheless, little is known on its potential mechanisms so far. Being regarded as a pivotal cytokine in the tumor microenvironment, transforming growth factor β (TGF-β) stands out as a robust regulator of epithelial-mesenchymal transition (EMT), which is closely linked to tumor progression. Aim of the study: The present study was designed to explore whether BFD antagonized EMT via blocking TGF-β1-induced signaling pathway, and then help contribute to create a relatively steady microenvironment for confining lung cancer. Materials and methods: This experiment was performed in lung adenocarcinoma A549 cells both in vitro and in vivo. In detail, the influences mediated by TGF-β1 alone or in combination with different concentrations of BFD on migration were detected by wound healing and transwell assays, and the effects of BFD on cell viability were determined by cell counting kit-8 (CCK-8) assay. TGF-β1, EMT relevant proteins and genes were evaluated by western blotting, confocal microscopy, quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and enzyme-linked immuno sorbent assay (ELISA). Female BALB/C nude mice were subcutaneously implanted A549 cells and given BFD by gavage twice daily for 28 days. The tumor volume was monitored every 4 days to draw growth curve. The tumor weight, expression levels of EMT-related protein in tumor tissues and TGF-β1 serum level were evaluated, respectively. Results: BFD only exerted minor effects on A549 cell proliferation and this was in accordance with the in vivo result, which showed that the tumor growth and weight were not be restrained by BFD administration. However, the data elucidated that BFD could dose-dependently suppress EMT induced by TGF-β1 in vitro via attenuating canonical Smad signaling pathway. In the A549 xenograft mouse model, BFD also inhibited protein markers that are associated with EMT and TGF-β1 secretion into serum. Conclusions: Based on these above data, the conclusion could be put forward that BFD probably attenuated TGF-β1 mediated EMT in A549 cells via decreasing canonical Smad signaling pathway both in vitro and in vivo, which may help restrain the malignant phenotype induced by TGF-β1 in A549 cells to some extent.
Language	English
Publishing date	2017-05-23
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2017.04.008
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Zs.A 1494: Show issues

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Article: Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo

He, Xi-Ran / Li-Na Pang / Ping-Ping Li / Shan-Tong Jiang / Shu-Yan Han / Wen-Xian Zheng / Xiao-Hong Li

Journal of ethnopharmacology. 2017 May 23, v. 204

2017

Abstract: Traditional Chinese medicine Bu-Fei decoction (BFD) has been utilized to treat patients with Qi ...

Abstract	Traditional Chinese medicine Bu-Fei decoction (BFD) has been utilized to treat patients with Qi deficiency for decades, with the advantages of invigorating vital energy, clearing heat-toxin and moistening lung, etc. According to previous clinical experience and trials, BFD has been found to indeed improve life quality of lung cancer patients and prolong survival time. Nevertheless, little is known on its potential mechanisms so far. Being regarded as a pivotal cytokine in the tumor microenvironment, transforming growth factor β (TGF-β) stands out as a robust regulator of epithelial-mesenchymal transition (EMT), which is closely linked to tumor progression.The present study was designed to explore whether BFD antagonized EMT via blocking TGF-β1-induced signaling pathway, and then help contribute to create a relatively steady microenvironment for confining lung cancer.This experiment was performed in lung adenocarcinoma A549 cells both in vitro and in vivo. In detail, the influences mediated by TGF-β1 alone or in combination with different concentrations of BFD on migration were detected by wound healing and transwell assays, and the effects of BFD on cell viability were determined by cell counting kit-8 (CCK-8) assay. TGF-β1, EMT relevant proteins and genes were evaluated by western blotting, confocal microscopy, quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and enzyme-linked immuno sorbent assay (ELISA). Female BALB/C nude mice were subcutaneously implanted A549 cells and given BFD by gavage twice daily for 28 days. The tumor volume was monitored every 4 days to draw growth curve. The tumor weight, expression levels of EMT-related protein in tumor tissues and TGF-β1 serum level were evaluated, respectively.BFD only exerted minor effects on A549 cell proliferation and this was in accordance with the in vivo result, which showed that the tumor growth and weight were not be restrained by BFD administration. However, the data elucidated that BFD could dose-dependently suppress EMT induced by TGF-β1 in vitro via attenuating canonical Smad signaling pathway. In the A549 xenograft mouse model, BFD also inhibited protein markers that are associated with EMT and TGF-β1 secretion into serum.Based on these above data, the conclusion could be put forward that BFD probably attenuated TGF-β1 mediated EMT in A549 cells via decreasing canonical Smad signaling pathway both in vitro and in vivo, which may help restrain the malignant phenotype induced by TGF-β1 in A549 cells to some extent.
Keywords	adenocarcinoma ; animal models ; blood serum ; cell proliferation ; cell viability ; confocal microscopy ; cytokines ; energy ; enzyme-linked immunosorbent assay ; females ; genes ; immunohistochemistry ; lung neoplasms ; mice ; Oriental traditional medicine ; patients ; phenotype ; proteins ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; secretion ; signal transduction ; sorbents ; tissue repair ; tissues ; transforming growth factor beta 1 ; Western blotting
Language	English
Dates of publication	2017-0523
Size	p. 45-57.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2017.04.008
Database	NAL-Catalogue (AGRICOLA)

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Article: [Preliminary study on differential expressed genes in T lymphocyte of chronic obstructive pulmonary disease patients with Fei-qi deficiency syndrome].

Li, Ze-Geng / Tong, Jia-Bing / Peng, Bo

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine

2006 Volume 26, Issue 12, Page(s) 1082–1085

Abstract: ... with chronic obstructive pulmonary disease (COPD) of Fei-qi deficiency (FQD) syndrome type by gene chips ... from COPD patients of FQD syndrome type, Fei-yin deficiency (FYD) syndrome type, and also from healthy ...

Abstract	Objective: To study T lymphocyte related genes with differential expression in patients with chronic obstructive pulmonary disease (COPD) of Fei-qi deficiency (FQD) syndrome type by gene chips. Methods: Lymphocytes in peripheral blood were isolated by Ficoll technique from blood samples collected from COPD patients of FQD syndrome type, Fei-yin deficiency (FYD) syndrome type, and also from healthy subjects for control. They were sorted and purified by flow cytometry, and the different expressed genes were screened from them by gene chip technique. Results: There were 15 genes with high differential expression between patients of FQD type and those of FYD syndrome type, and between patients of FQD type and healthy subjects. Conclusion: Gene chip technique could be used for studying the gene expression profiles of TCM syndrome, and the T-lymphocyte related genes with differential expression in COPD patients with FQD were screened preliminarily.
MeSH term(s)	Adult ; Diagnosis, Differential ; Female ; Gene Expression Profiling ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Oligonucleotide Array Sequence Analysis/methods ; Pulmonary Disease, Chronic Obstructive/complications ; Pulmonary Disease, Chronic Obstructive/genetics ; Pulmonary Disease, Chronic Obstructive/immunology ; Syndrome ; T-Lymphocytes/metabolism ; Yang Deficiency/complications ; Yang Deficiency/immunology
Language	Chinese
Publishing date	2006-12
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1195456-5
ISSN	1003-5370
ISSN	1003-5370
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Article: Identification and validation of potential biomarkers for atrial fibrillation based on integrated bioinformatics analysis.

Tong, Fei / Sun, Zhijun

Frontiers in cell and developmental biology

2024 Volume 11, Page(s) 1190273

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2024-01-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2023.1190273
Database	MEDical Literature Analysis and Retrieval System OnLINE

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