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Article ; Online: Remarkable stability of tetrabenzo[a,c,g,i]fluorenyl ammonium salts in water: syntheses, reactions, and crystal structures.

Frey, Okko N / Stalling, Timo / Schlüter, Florian / Saak, Wolfgang / Schmidtmann, Marc / Haase, Detlev / Beckhaus, Rüdiger

Dalton transactions (Cambridge, England : 2003)

2016 Volume 45, Issue 3, Page(s) 1085–1092

Abstract: Deprotonation of 8bH-tetrabenzo[a,c,g,i]fluorene (8bH-Tbf), an extremely large benzannulated ...

Abstract	Deprotonation of 8bH-tetrabenzo[a,c,g,i]fluorene (8bH-Tbf), an extremely large benzannulated cyclopentadienyl derivative, by [NR3R'][OH] (R = (n)Bu, Et; R' = (n)Bu, Et, Bn) leads to a series of Tbf ammonium salts of the type [NR3R'][Tbf]. These cyclopentadienide analogs were smoothly isolated from a hydrous medium and structurally characterized by single X-ray diffraction. Subsequent reactions demonstrate the high potential of derivatization by converting the presented [Tbf] anions into functionalized Tbf compounds.
Language	English
Publishing date	2016-01-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/c5dt04137f
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Article ; Online: Modulation of distinct isoforms of L-type calcium channels by G(q)-coupled receptors in Xenopus oocytes: antagonistic effects of Gβγ and protein kinase C.

Weiss, Sharon / Keren-Raifman, Tal / Oz, Shimrit / Ben Mocha, Adva / Haase, Hannelore / Dascal, Nathan

Channels (Austin, Tex.)

2012 Volume 6, Issue 6, Page(s) 426–437

Abstract: ... physiology. In heart and smooth muscle, hormones and transmitters operating via G(q) enhance L-VDCC currents ... via essential protein kinase C (PKC) involvement. Heterologous reconstitution studies in Xenopus ... oocytes suggested that PKC and G(q)-coupled receptors increased L-VDCC currents only in cardiac long N ...

Abstract	L-type voltage dependent Ca(2+) channels (L-VDCCs; Ca(v)1.2) are crucial in cardiovascular physiology. In heart and smooth muscle, hormones and transmitters operating via G(q) enhance L-VDCC currents via essential protein kinase C (PKC) involvement. Heterologous reconstitution studies in Xenopus oocytes suggested that PKC and G(q)-coupled receptors increased L-VDCC currents only in cardiac long N-terminus (NT) isoforms of α(1C), whereas known smooth muscle short-NT isoforms were inhibited by PKC and G(q) activators. We report a novel regulation of the long-NT α(1C) isoform by Gβγ. Gβγ inhibited whereas a Gβγ scavenger protein augmented the G(q)--but not phorbol ester-mediated enhancement of channel activity, suggesting that Gβγ acts upstream from PKC. In vitro binding experiments reveal binding of both Gβγ and PKC to α(1C)-NT. However, PKC modulation was not altered by mutations of multiple potential phosphorylation sites in the NT, and was attenuated by a mutation of C-terminally located serine S1928. The insertion of exon 9a in intracellular loop 1 rendered the short-NT α(1C) sensitive to PKC stimulation and to Gβγ scavenging. Our results suggest a complex antagonistic interplay between G(q)-activated PKC and Gβγ in regulation of L-VDCC, in which multiple cytosolic segments of α(1C) are involved.
MeSH term(s)	Animals ; Binding Sites ; Calcium Channels, L-Type/chemistry ; Calcium Channels, L-Type/metabolism ; Chloride Channels/metabolism ; Enzyme Activation/drug effects ; Exons/genetics ; GTP-Binding Protein alpha Subunits, Gq-G11/metabolism ; GTP-Binding Protein beta Subunits/metabolism ; GTP-Binding Protein gamma Subunits/metabolism ; Humans ; Ion Channel Gating/drug effects ; Mutation/genetics ; Oocytes/drug effects ; Oocytes/metabolism ; Phosphorylation/drug effects ; Protein Binding/drug effects ; Protein Isoforms/chemistry ; Protein Isoforms/metabolism ; Protein Kinase C/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rabbits ; Rats ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Muscarinic/metabolism ; Tetradecanoylphorbol Acetate/pharmacology ; Up-Regulation/drug effects ; Xenopus laevis/metabolism
Chemical Substances	Calcium Channels, L-Type ; Chloride Channels ; G-protein Beta gamma ; GTP-Binding Protein beta Subunits ; GTP-Binding Protein gamma Subunits ; Protein Isoforms ; RNA, Messenger ; Receptors, G-Protein-Coupled ; Receptors, Muscarinic ; Protein Kinase C (EC 2.7.11.13) ; GTP-Binding Protein alpha Subunits, Gq-G11 (EC 3.6.5.1) ; Tetradecanoylphorbol Acetate (NI40JAQ945)
Language	English
Publishing date	2012-09-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2262854-X
ISSN	1933-6969 ; 1933-6969
ISSN (online)	1933-6969
ISSN	1933-6969
DOI	10.4161/chan.22016
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Article ; Online: White-nose syndrome, winter duration, and pre-hibernation climate impact abundance of reproductive female bats.

Krueger, Sarah K / Williams, Sarah C / O'Keefe, Joy M / Zirkle, Gene A / Haase, Catherine G

PloS one

2024 Volume 19, Issue 4, Page(s) e0298515

Abstract: White-nose syndrome (WNS) is an infectious disease that disrupts hibernation in bats, leading to premature exhaustion of fat stores. Though we know WNS does impact reproduction in hibernating female bats, we are unsure how these impacts are exacerbated ... ...

Abstract	White-nose syndrome (WNS) is an infectious disease that disrupts hibernation in bats, leading to premature exhaustion of fat stores. Though we know WNS does impact reproduction in hibernating female bats, we are unsure how these impacts are exacerbated by local climate factors. We compiled data from four southeastern U.S. states and used generalized linear mixed effects models to compare effects of WNS, pre-hibernation climate variables, and winter duration on the number of reproductive females in species across the range of WNS susceptibility. We predicted we would see a decline in the number of reproductive females in WNS-susceptible species, with the effect exaggerated by longer winter durations and pre-hibernation climate variables that lead to reductions in foraging. We found that the number of reproductive females in WNS-susceptible species was positively correlated with pre-hibernation local climate conditions conducive to foraging; however, WNS-susceptible species experienced an overall decline with the presence of WNS and as winter duration increased. Our long-term dataset provides evidence that pre-hibernation climate, specifically favorable summer weather conditions for foraging, greatly influences the reproduction, regardless of WNS status.
MeSH term(s)	Animals ; Chiroptera/physiology ; Female ; Hibernation/physiology ; Seasons ; Reproduction/physiology ; Climate ; Mycoses/veterinary ; Mycoses/epidemiology ; White
Language	English
Publishing date	2024-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0298515
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Article ; Online: Cluster analysis of volatile organic compounds - A pilot study in patients with severe acute decompensated heart failure.

Butter, Lukas / Haase-Fielitz, Anja / Zänker, Michael / Becher, Gunther / Nübel, Jonathan / Butter, Christian

Journal of cardiology

2024

Abstract: Introduction: Acute decompensated heart failure (ADHF) is a global health problem and early detection of high-risk patients for effective treatment is important. Exhaled breath analysis and measurement of volatile organic compounds (VOCs) may be a fast ... ...

Abstract	Introduction: Acute decompensated heart failure (ADHF) is a global health problem and early detection of high-risk patients for effective treatment is important. Exhaled breath analysis and measurement of volatile organic compounds (VOCs) may be a fast and cost-effective non-invasive diagnostic and screening tool complementing measurement of cardiac biomarkers. Another technique to detect and characterize VOCs is the ion mobility spectrometry (IMS) not requiring vacuum or sample pretreatment. Methods: This prospective controlled proof-of-concept study prospectively enrolled adult patients with severe ADHF at the University Hospital Heart Centre Brandenburg. Severe ADHF was defined as patients presenting with symptomatic acute decompensation and NTproBNP >7000 pg/dL. Cardiac patients with NT-proBNP 220 pg/dL served as control. A gas chromatography ion mobility spectrometer (GC-IMS) of the type "MultiMarkerMonitor™" from GRAUPNER medical solutions GmbH was used. Measurement was performed at T0 (within 24 h of admission), T1 (after 3-5 days) and T2 (after 8-10 days). Results: Forty patients were enrolled in the study, 20 patients with severe ADHF and 20 control patients. In patients with severe ADHF, three clusters with significantly altered maximum peak heights were detected compared to control. There was no change in the peak height of clusters 8, 9 and 206 at the time points T1 and T2 (all p > 0.50). Also, NT-proBNP was stable over time (p = 0.247). Sixteen control patients (16/20, 80 %) and four with severe ADHF (4/20, 20 %) presented without cluster deviation. Patients with deviation in at least two clusters had longer hospital stay, 11 days (5.0-15.0) compared to those without deviation, 4 days (2.0-9.5), p = 0.028. Conclusion: Longer-term follow-up studies are needed to assess the stability and clinical significance of the identified clusters by IMS and their diagnostic and prognostic relevance.
Language	English
Publishing date	2024-04-15
Publishing country	Netherlands
Document type	Letter
ZDB-ID	639364-0
ISSN	1876-4738 ; 0386-2887 ; 0914-5087
ISSN (online)	1876-4738
ISSN	0386-2887 ; 0914-5087
DOI	10.1016/j.jjcc.2024.04.003
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Article ; Online: De-novo and genome-wide meta-analyses identify a risk haplotype for congenital sensorineural deafness in Dalmatian dogs.

Haase, B / Willet, C E / Chew, T / Samaha, G / Child, G / Wade, C M

Scientific reports

2022 Volume 12, Issue 1, Page(s) 15439

Abstract: ... markers (BICF2G630233852T>C, BICF2G630233861T>C, BICF2G630233888G>A) on CFA20 with 62% of bilaterally deaf ...

Abstract	Congenital sensorineural deafness (CSD) has been reported to affect up to 30% of Dalmatian dogs world-wide and while unilaterally deaf dogs can live a close to normal life, dogs suffering bilateral deafness are frequently euthanized. Extreme-white coat patterning as encoded by the gene Melanocyte Inducing Transcription Factor (MITF) has long been postulated as the major risk factor for CSD in the Dalmatian breed. While attempts to identify causative risk variants associated with CSD have been numerous, no genome-wide association study has positively identified MITF as a risk locus for either bilateral or unilateral deafness in the Dalmatian breed to date. In this study, we identified an association with CSD on CFA20 in the vicinity of MITF within Australian Dalmatian dogs. Although not genome-wide significant, the association signal was validated by reanalysing publicly available data and merging the wider data resource with the local data to improve statistical power. The merged data, representing three major global populations of Dalmatian dogs, enabled us to identify a single, well-defined genome-wide significant risk haplotype for CSD. The haplotype was formed by three genome-wide significant associated markers (BICF2G630233852T>C, BICF2G630233861T>C, BICF2G630233888G>A) on CFA20 with 62% of bilaterally deaf dogs homozygous for the risk haplotype (CCA), while 30% of bilaterally deaf and 45% of hearing dogs carried one copy of the risk haplotype. Animals homozygous or heterozygous for the low-risk haplotype were less likely to be unilaterally deaf. While the association between the risk haplotype and deafness is incomplete, animals homozygous for the risk haplotype were 10-times more likely to be bilaterally deaf. Although the underlying causative variants are yet to be discovered, results from this study can now assist with reducing deafness in Dalmatian dogs.
MeSH term(s)	Animals ; Australia ; Deafness/genetics ; Deafness/veterinary ; Dog Diseases/genetics ; Dogs ; Haplotypes ; Hearing Loss, Sensorineural/congenital ; Hearing Loss, Sensorineural/genetics ; Hearing Loss, Sensorineural/veterinary
Language	English
Publishing date	2022-09-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-19535-4
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Article ; Online: Patient-reported outcomes in large vessel vasculitis: insights from a retrospective analysis of disease activity and associated factors.

Kernder, A / Rohde, M / Acar, H / Düsing, C / Fischer-Betz, R / Haase, I / Mucke, J / Sander, O / Richter, J G / Filla, T / Schneider, M / Chehab, G

Journal of patient-reported outcomes

2024 Volume 8, Issue 1, Page(s) 4

Abstract: ... Patient and physician-rated global disease activity scale (G-DAS) scores, measured on a numeric rating ... up was 2.5 years (0-7.7). Patient and physician G-DAS exhibited a moderate correlation (Pearson R 0 ... 19, CI 0.14-0.24, p < 0.001). Higher patient G-DAS correlated with younger age (CI -3.4 - -1.5, p < 0 ...

Abstract	Background: Patient-reported outcomes (PROs) play a crucial role in assessing rheumatic diseases, offering insights into disease evaluation and treatment efficacy. This study focuses on PRO assessment in large vessel vasculitides, including Takayasu Arteritis and Giant Cell Arteritis (GCA). Methods: We retrospectively analyzed routine data from patients treated at our rheumatology clinic over a 10-year span. Patient and physician-rated global disease activity scale (G-DAS) scores, measured on a numeric rating scale (0-10 points), were collected at each visit. Clinical variables like age, sex, body mass index (BMI), disease duration, lab values, pain perception, and questionnaire responses were recorded. Linear regression and generalized additive linear regression (GAM analysis) examined associations between PROs and these factors. Results: The study included 138 patients, primarily diagnosed with GCA (94.4%). Mean follow-up was 2.5 years (0-7.7). Patient and physician G-DAS exhibited a moderate correlation (Pearson R 0.19, CI 0.14-0.24, p < 0.001). Higher patient G-DAS correlated with younger age (CI -3.4 - -1.5, p < 0.001), increased pain (CI 3.5-4, p < 0.001), functional limitations (HAQ, CI 0.5-0.6, p < 0.001), reduced physical (CI 2.3-2.7, p ≤ 0.001) and psychological well-being (CI 2.1-2.5, p < 0.001), and higher BMI (CI 1.3-2.4, p < 0.001). Physician G-DAS correlated with Birmingham Vasculitis Activity Score (V3.0; R 0.42, p 0.046) and were significantly linked to serum CRP elevations (β = 0.04, CI 0.0-0.08, p 0.028). Conclusions: These findings underscore the need to integrate PRO measures into vasculitis disease management strategies, enhancing the understanding of disease activity from the patient's perspective.
MeSH term(s)	Humans ; Retrospective Studies ; Giant Cell Arteritis/diagnosis ; Takayasu Arteritis/diagnosis ; Patient Reported Outcome Measures ; Ambulatory Care Facilities
Language	English
Publishing date	2024-01-08
Publishing country	Germany
Document type	Journal Article
ISSN	2509-8020
ISSN (online)	2509-8020
DOI	10.1186/s41687-023-00681-w
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Book ; Online: Lower Bounds on the Depth of Integral ReLU Neural Networks via Lattice Polytopes

Haase, Christian / Hertrich, Christoph / Loho, Georg

2023

Abstract: We prove that the set of functions representable by ReLU neural networks with integer weights strictly increases with the network depth while allowing arbitrary width. More precisely, we show that $\lceil\log_2(n)\rceil$ hidden layers are indeed ... ...

Abstract	We prove that the set of functions representable by ReLU neural networks with integer weights strictly increases with the network depth while allowing arbitrary width. More precisely, we show that $\lceil\log_2(n)\rceil$ hidden layers are indeed necessary to compute the maximum of $n$ numbers, matching known upper bounds. Our results are based on the known duality between neural networks and Newton polytopes via tropical geometry. The integrality assumption implies that these Newton polytopes are lattice polytopes. Then, our depth lower bounds follow from a parity argument on the normalized volume of faces of such polytopes. Comment: ICLR 2023 conference paper
Keywords	Computer Science - Machine Learning ; Computer Science - Discrete Mathematics ; Computer Science - Neural and Evolutionary Computing ; Mathematics - Combinatorics ; Statistics - Machine Learning
Publishing date	2023-02-24
Publishing country	us
Document type	Book ; Online
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Article ; Online: Comparison of 3D and 4D robustly optimized proton treatment plans for non-small cell lung cancer patients with tumour motion amplitudes larger than 5 mm.

Spautz, Saskia / Haase, Leon / Tschiche, Maria / Makocki, Sebastian / Richter, Christian / Troost, Esther G C / Stützer, Kristin

Physics and imaging in radiation oncology

2023 Volume 27, Page(s) 100465

Abstract: Background and purpose: There is no consensus about an ideal robust optimization (RO) strategy for proton therapy of targets with large intrafractional motion. We investigated the plan robustness of 3D and different 4D RO strategies.: Materials and ... ...

Abstract	Background and purpose: There is no consensus about an ideal robust optimization (RO) strategy for proton therapy of targets with large intrafractional motion. We investigated the plan robustness of 3D and different 4D RO strategies. Materials and methods: For eight non-small cell lung cancer patients with clinical target volume (CTV) motion >5 mm, different RO approaches were investigated: 3DRO considering the average CT (AvgCT) with a target density override, 4DRO considering three/all 4DCT phases, and 4DRO considering the AvgCT and three/all 4DCT phases. Robustness against setup/range errors, interplay effects based on breathing and machine log file data for deliveries with/without rescanning, and interfractional anatomical changes were analyzed for target coverage and OAR sparing. Results: All nominal plans fulfilled the clinical requirements with individual CTV coverage differences <2pp; 4DRO without AvgCT generated the most conformal dose distributions. Robustness against setup/range errors was best for 4DRO with AvgCT (18% more passed error scenarios than 3DRO). Interplay effects caused fraction-wise median CTV coverage loss of 3pp and missed maximum dose constraints for heart and esophagus in 18% of scenarios. CTV coverage and OAR sparing fulfilled requirements in all cases when accumulating four interplay scenarios. Interfractional changes caused less target misses for RO with AvgCT compared to 4DRO without AvgCT (≤42%/33% vs. ≥56%/44% failed single/accumulated scenarios). Conclusions: All RO strategies provided acceptable plans with equally low robustness against interplay effects demanding other mitigation than rescanning to ensure fraction-wise target coverage. 4DRO considering three phases and the AvgCT provided best compromise on planning effort and robustness.
Language	English
Publishing date	2023-06-24
Publishing country	Netherlands
Document type	Journal Article
ISSN	2405-6316
ISSN (online)	2405-6316
DOI	10.1016/j.phro.2023.100465
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Article: App-based assessment of memory functions in patients after transfemoral aortic valve replacement.

Nübel, Jonathan / Hauptmann, Michael / Schön, Julika / Fritz, Georg / Butter, Christian / Haase-Fielitz, Anja

Journal of geriatric cardiology : JGC

2023 Volume 20, Issue 9, Page(s) 664–672

Abstract: Background: Transfemoral aortic valve replacement (TAVR) is the standard treatment for elderly patients with aortic valve stenosis. Although safe and well-established, there is a risk of intraprocedural hemodynamic instability and silent cerebral ... ...

Abstract	Background: Transfemoral aortic valve replacement (TAVR) is the standard treatment for elderly patients with aortic valve stenosis. Although safe and well-established, there is a risk of intraprocedural hemodynamic instability and silent cerebral embolism, which can lead to a decline in neurocognitive function and dementia. In clinical practice, comprehensive cognitive testing is difficult to perform. AI-assisted digital applications may help to optimize diagnosis and monitoring. Methods: Neurocognitive function was assessed by validated psychometric tests using "∆elta -App", which uses artificial intelligence and computational linguistic methods for extraction and analysis. Memory function was assessed using the 'Consortium to Establish a Registry for Alzheimer's Disease' (CERAD) word list and digit span task (DST) before TAVR and before hospital discharge. The study is registered in the German Register of Clinical Trials (https://drks.de/search/de/trial/DRKS00020813). Results: From October 2020 until March 2022, 141 patients were enrolled at University Hospital Heart Centre Brandenburg. Mean age was 81 ± 6 years, 42.6% were women. Time between the pre- and post-interventional test was on average 6 ± 3 days. Memory function before TAVR was found to be below average in relation to age and educational level. The pre-post TAVR comparison showed significant improvements in the wordlist repeat, Conclusions: Despite impaired preoperative memory function before TAVR, no global negative effect on memory function after TVAR was detected. The improvements shown in the word list test should be interpreted as usual learning effects in this task.
Language	English
Publishing date	2023-08-01
Publishing country	China
Document type	Journal Article
ZDB-ID	2421391-3
ISSN	1671-5411
ISSN	1671-5411
DOI	10.26599/1671-5411.2023.09.004
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Article ; Online: The new WHO 2022 and ICC proposals for the classification of myelodysplastic neoplasms. Validation based on the Düsseldorf MDS Registry and proposals for a merged classification.

Nachtkamp, K / Strupp, C / Vukelja, M / Kasprzak, A / Haase, D / Ganster, C / Hildebrandt, B / Betz, B / Giagounidis, A / Aul, C / Blum, S / Hofmann, W K / Pfeilstöcker, M / Valent, P / Lübbert, M / Seidl, M / Rudelius, M / Stauder, R / Krieger, O /

Götze, K S / Bobak, J / Kündgen, A / Schulz, F / Dietrich, S / Kobbe, G / Gattermann, N / Germing, U

Leukemia

2024 Volume 38, Issue 2, Page(s) 442–445

MeSH term(s)	Humans ; Neoplasms ; Myelodysplastic Syndromes ; Registries ; World Health Organization
Language	English
Publishing date	2024-01-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	807030-1
ISSN	1476-5551 ; 0887-6924
ISSN (online)	1476-5551
ISSN	0887-6924
DOI	10.1038/s41375-024-02157-2
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Zs.A 2295: Show issues

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