LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 1321

Search options

  1. Article ; Online: Conformational equilibrium shift underlies altered K

    Iwahashi, Yuta / Toyama, Yuki / Imai, Shunsuke / Itoh, Hiroaki / Osawa, Masanori / Inoue, Masayuki / Shimada, Ichio

    Nature communications

    2020  Volume 11, Issue 1, Page(s) 5168

    Abstract: The potassium ion (K ...

    Abstract The potassium ion (K
    MeSH term(s) Alanine/genetics ; Ataxia/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/isolation & purification ; Bacterial Proteins/metabolism ; Epilepsy/genetics ; Humans ; Ion Channel Gating/genetics ; Long QT Syndrome/genetics ; Magnetic Resonance Spectroscopy ; Molecular Dynamics Simulation ; Mutation, Missense ; Potassium/metabolism ; Potassium Channels/genetics ; Potassium Channels/isolation & purification ; Potassium Channels/metabolism ; Protein Conformation, alpha-Helical/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Streptomyces lividans ; Valine/genetics
    Chemical Substances Bacterial Proteins ; Potassium Channels ; Recombinant Proteins ; prokaryotic potassium channel ; Valine (HG18B9YRS7) ; Alanine (OF5P57N2ZX) ; Potassium (RWP5GA015D)
    Language English
    Publishing date 2020-10-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-020-19005-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR

    Yuta Iwahashi / Yuki Toyama / Shunsuke Imai / Hiroaki Itoh / Masanori Osawa / Masayuki Inoue / Ichio Shimada

    Nature Communications, Vol 11, Iss 1, Pp 1-

    2020  Volume 11

    Abstract: Potassium ion channels control K+ permeation across cell membranes and mutations that cause ... cardiovascular and neural diseases are known. Here, the authors perform NMR measurements with the prototypical K+ ... on the conformational dynamics of K+ channels in a physiological solution environment. ...

    Abstract Potassium ion channels control K+ permeation across cell membranes and mutations that cause cardiovascular and neural diseases are known. Here, the authors perform NMR measurements with the prototypical K+ channel from Streptomyces lividans, KcsA and characterise the effects of disease causing mutations on the conformational dynamics of K+ channels in a physiological solution environment.
    Keywords Science ; Q
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Characterization of an etoposide-resistant human K562 cell line, K/eto.

    Sugawara, I / Iwahashi, T / Okamoto, K / Sugimoto, Y / Ekimoto, H / Tsuruo, T / Ikeuchi, T / Mori, S

    Japanese journal of cancer research : Gann

    1991  Volume 82, Issue 9, Page(s) 1035–1043

    Abstract: An etoposide-resistant K562 cell line (K/eto) was obtained by stepwise exposure, in culture ... compared with parental K562. Karyotype analysis showed that the number of chromosomes in K/eto was 55 and ...

    Abstract An etoposide-resistant K562 cell line (K/eto) was obtained by stepwise exposure, in culture, to increasing concentrations of etoposide, without the use of mutagens. This cell line was resistant to etoposide, and slightly resistant to adriamycin, but sensitive to anti-cancer drugs such as camptothecin, vincristine, actinomycin D and so on. P-Glycoprotein, the mdr1 gene product, was not detected in this cell line, as assessed by immunocytochemistry, immunoprecipitation and flow cytometry. Overexpression of mdr1 mRNA was also not found. Interestingly, expression of 85 kD protein recognized by MRK 20 monoclonal antibody was noted. The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected. Cellular accumulation of [3H]etoposide was reduced by 75% in the resistant line compared with parental K562. Karyotype analysis showed that the number of chromosomes in K/eto was 55 and neither a homogeneous staining region nor double-minute chromosomes were detected. These results indicate that this resistance is not due to an altered interaction between the drug and cellular transport machinery, i.e. MDR1, associated with the "classic" multiple drug resistance phenotype, but rather is due to the existence of other mechanism(s) of resistance, decreased transport of the drug and decreased target enzyme, DNA topoisomerase II.
    MeSH term(s) Antibodies, Monoclonal ; DNA Topoisomerases, Type I/immunology ; DNA Topoisomerases, Type I/metabolism ; DNA Topoisomerases, Type II/immunology ; DNA Topoisomerases, Type II/metabolism ; Drug Resistance/genetics ; Etoposide/pharmacokinetics ; Etoposide/pharmacology ; Humans ; Leukemia, Myeloid/genetics ; Leukemia, Myeloid/metabolism ; Leukemia, Myeloid/pathology ; Tritium ; Tumor Cells, Cultured
    Chemical Substances Antibodies, Monoclonal ; Tritium (10028-17-8) ; Etoposide (6PLQ3CP4P3) ; DNA Topoisomerases, Type I (EC 5.99.1.2) ; DNA Topoisomerases, Type II (EC 5.99.1.3)
    Language English
    Publishing date 1991-09
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 804498-3
    ISSN 0910-5050
    ISSN 0910-5050
    DOI 10.1111/j.1349-7006.1991.tb01940.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.14: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: The rising threat of Mpox in Japan despite global decrease.

    Kaneda, Yudai / Shrestha, Rajeev / Iwahashi, Kota / Takahashi, Kenzo

    Journal of public health policy

    2023  Volume 44, Issue 3, Page(s) 499–502

    MeSH term(s) Humans ; Japan ; Mpox (monkeypox)
    Language English
    Publishing date 2023-08-24
    Publishing country England
    Document type Letter
    ZDB-ID 603208-4
    ISSN 1745-655X ; 0197-5897
    ISSN (online) 1745-655X
    ISSN 0197-5897
    DOI 10.1057/s41271-023-00427-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1767: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: K-252a, a potent inhibitor of protein kinase C from microbial origin.

    Kase, H / Iwahashi, K / Matsuda, Y

    The Journal of antibiotics

    1986  Volume 39, Issue 8, Page(s) 1059–1065

    Abstract: K-252a, a metabolite isolated from the culture broth of Nocardiopsis sp. K-252a, was found ...

    Abstract K-252a, a metabolite isolated from the culture broth of Nocardiopsis sp. K-252a, was found to exhibit an extremely potent inhibitory activity on protein kinase C. The IC50 value was 32.9 nM.
    MeSH term(s) Actinomycetales/metabolism ; Animals ; Bacteria/drug effects ; Calmodulin/antagonists & inhibitors ; Carbazoles/isolation & purification ; Carbazoles/pharmacology ; Fermentation ; Indole Alkaloids ; Mice ; Phosphodiesterase Inhibitors/pharmacology ; Protein Kinase C/antagonists & inhibitors
    Chemical Substances Calmodulin ; Carbazoles ; Indole Alkaloids ; Phosphodiesterase Inhibitors ; staurosporine aglycone (97161-97-2) ; Protein Kinase C (EC 2.7.11.13)
    Language English
    Publishing date 1986-08
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 390800-8
    ISSN 1881-1469 ; 0021-8820 ; 0368-3532
    ISSN (online) 1881-1469
    ISSN 0021-8820 ; 0368-3532
    DOI 10.7164/antibiotics.39.1059
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 207: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Long-Term Visual Prognosis of Patients Following Lens-Sparing Vitrectomy for Stage 4A Retinopathy of Prematurity.

    Iwahashi, Chiharu / Kurihara, Tomoki / Kuniyoshi, Kazuki / Kusaka, Shunji

    International journal of molecular sciences

    2023  Volume 24, Issue 3

    Abstract: This study evaluated the long-term visual outcomes of patients in whom at least one eye underwent successful lens-sparing vitrectomy (LSV) for stage 4A retinopathy of prematurity (ROP). A retrospective chart review was conducted using the data of 61 eyes ...

    Abstract This study evaluated the long-term visual outcomes of patients in whom at least one eye underwent successful lens-sparing vitrectomy (LSV) for stage 4A retinopathy of prematurity (ROP). A retrospective chart review was conducted using the data of 61 eyes of 42 patients with a minimum 4-year follow-up after successful LSV, with or without anti-vascular endothelial growth factor (VEGF) therapy, and whose best-corrected visual acuity (BCVA) was measurable using Landolt rings at the final visit. The mean age at the final follow-up was 10.1 ± 3.3 years. Before LSV, all eyes underwent laser ablation therapy. Twenty eyes (32.8%) with high vascular activity received anti-VEGF therapy before LSV. The mean decimal BCVA at the final follow-up was 0.23 ± 0.26 (range: hand motion to 1.2). Twenty-three eyes (54.1%) had a decimal BCVA of ≥0.4. Among 49 phakic eyes at the final examination, the mean refractive error was -10.1 ± 5.0 D, with 37 eyes (75.5%) having high myopia (>-6.0 D). No significant differences were observed in terms of decimal BCVA and refractive errors between eyes with and without anti-VEGF therapy. Approximately half of the patients had a decimal BCVA of ≥0.4, despite myopic refraction after successful LSV for stage 4A ROP. LSV for stage 4A ROP seemed to be associated with good visual function, despite myopic refraction.
    MeSH term(s) Infant, Newborn ; Humans ; Child ; Adolescent ; Vitrectomy/adverse effects ; Retinopathy of Prematurity/surgery ; Retrospective Studies ; Visual Acuity ; Treatment Outcome ; Myopia/complications ; Infant, Newborn, Diseases/etiology ; Follow-Up Studies ; Gestational Age
    Language English
    Publishing date 2023-01-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032416
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: Prevention and management of critical care complications in cardiogenic shock: a narrative review.

    Kirigaya, Jin / Iwahashi, Noriaki / Terasaka, Kengo / Takeuchi, Ichiro

    Journal of intensive care

    2023  Volume 11, Issue 1, Page(s) 31

    Abstract: Background: Cardiogenic shock (CS) is a common cause of morbidity and mortality in cardiac intensive care units (CICUs), even in the contemporary era.: Main text: Although mechanical circulatory supports have recently become widely available and used ...

    Abstract Background: Cardiogenic shock (CS) is a common cause of morbidity and mortality in cardiac intensive care units (CICUs), even in the contemporary era.
    Main text: Although mechanical circulatory supports have recently become widely available and used in transforming the management of CS, their routine use to improve outcomes has not been established. Transportation to a high-volume center, early reperfusion, tailored mechanical circulatory supports, regionalized systems of care with multidisciplinary CS teams, a dedicated CICU, and a systemic approach, including preventing noncardiogenic complications, are the key components of CS treatment strategies.
    Conclusions: This narrative review aimed to discuss the challenges of preventing patients from developing CS-related complications and provide a comprehensive practical approach for its management.
    Language English
    Publishing date 2023-07-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2739853-5
    ISSN 2052-0492
    ISSN 2052-0492
    DOI 10.1186/s40560-023-00675-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Community-Based Trial Educational Heat Disorder Program in Local Salons for Older Adults.

    Kuniyoshi, Hikaru / Iwahashi, Yoshiko / Watanabe, Koichi

    Journal of UOEH

    2023  Volume 45, Issue 3, Page(s) 143–153

    Abstract: We investigated whether a combined program for heat disorder prevention can improve the behaviors and knowledge of heat disorder prevention in older adults participating in salons. This non-randomized parallel-group comparison study includes 59 ... ...

    Abstract We investigated whether a combined program for heat disorder prevention can improve the behaviors and knowledge of heat disorder prevention in older adults participating in salons. This non-randomized parallel-group comparison study includes 59 participants from two salons. The intervention group met on alternate weeks on a salon day to watch an educational DVD and conduct hands-on education (Wet-Bulb Globe Temperature (WBGT) measurements inside and outside the meeting place). The intervention group conducted calls every other week except salon week to prevent action for heat disorder prevention information based on a leaflet and monitored WBGT in their bedrooms daily at bedtime. We assessed changes in recognition of heat disorders and prevention behavior between baseline, one and a half months after intervention, and post-season (approximately two months after baseline), using the Wilcoxon signed rank test. Recognition of heat disorder scores and preventive behavior improved from pre- to post-season in the intervention group. It was suggested that the approach toward older adults participating in salons could improve their recognition and behavior in preventing heat disorders.
    MeSH term(s) Aged ; Humans ; Hot Temperature ; Knowledge ; Seasons ; Heat Stress Disorders/prevention & control ; Health Promotion ; Patient Education as Topic
    Language English
    Publishing date 2023-08-24
    Publishing country Japan
    Document type Comparative Study ; Journal Article
    ZDB-ID 632724-2
    ISSN 2187-2864 ; 0387-821X
    ISSN (online) 2187-2864
    ISSN 0387-821X
    DOI 10.7888/juoeh.45.143
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2868: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: The effect of K-252a, a potent microbial inhibitor of protein kinase, on activated cyclic nucleotide phosphodiesterase.

    Matsuda, Y / Nakanishi, S / Nagasawa, K / Iwahashi, K / Kase, H

    The Biochemical journal

    1988  Volume 256, Issue 1, Page(s) 75–80

    Abstract: K-252a, an indole carbazol compound of microbial origin, inhibited activation of bovine brain ... by K-252a with respect to CaM. On the other hand, inhibition of the trypsin-activated phosphodiesterase ... compound 48/80, or calmidazolium but, in contrast, had no effect on the inhibition by K-252a. Furthermore ...

    Abstract K-252a, an indole carbazol compound of microbial origin, inhibited activation of bovine brain phosphodiesterase induced by calmodulin (CaM), sodium oleate, or limited proteolysis with almost equal potency. Kinetic analysis revealed that the CaM-activated phosphodiesterase (CaM-PDE) was competitively inhibited by K-252a with respect to CaM. On the other hand, inhibition of the trypsin-activated phosphodiesterase was competitive with respect to cyclic AMP. Addition of a lower amount of phosphatidylserine or sodium oleate to the reaction medium was efficacious in attenuating the inhibition of the CaM-PDE by W-7, compound 48/80, or calmidazolium but, in contrast, had no effect on the inhibition by K-252a. Furthermore, CaM-independent systems such as [3H]nitrendipine receptor binding or Na+ + K+-ATPase were influenced less by K-252a compared with W-7, compound 48/80 and calmidazolium. In conclusion, K-252a is an inhibitor of CaM-dependent cyclic nucleotide phosphodiesterase and it appears that it inhibits the enzyme not only via CaM antagonism but possibly also by interfering with the enzyme.
    MeSH term(s) 2',3'-Cyclic-Nucleotide Phosphodiesterases/antagonists & inhibitors ; Animals ; Binding, Competitive ; Brain/enzymology ; Calmodulin/pharmacology ; Carbazoles/pharmacology ; Cattle ; Enzyme Activation/drug effects ; Indole Alkaloids ; Kinetics ; Oleic Acid ; Oleic Acids/pharmacology ; Phosphatidylserines/pharmacology ; Protein Kinase Inhibitors ; Trypsin/pharmacology
    Chemical Substances Calmodulin ; Carbazoles ; Indole Alkaloids ; Oleic Acids ; Phosphatidylserines ; Protein Kinase Inhibitors ; Oleic Acid (2UMI9U37CP) ; osteum (399SL044HN) ; staurosporine aglycone (97161-97-2) ; 2',3'-Cyclic-Nucleotide Phosphodiesterases (EC 3.1.4.-) ; Trypsin (EC 3.4.21.4)
    Language English
    Publishing date 1988-11-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0264-6021 ; 0006-2936 ; 0306-3275
    ISSN (online) 1470-8728
    ISSN 0264-6021 ; 0006-2936 ; 0306-3275
    DOI 10.1042/bj2560075
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.110: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Worsening Dyspnea in Patients With Idiopathic Portal Hypertension.

    Iwahashi, Noriaki / Horii, Mutsuo / Tamura, Kouichi / Kimura, Kazuo

    Chest

    2022  Volume 161, Issue 4, Page(s) e245–e248

    MeSH term(s) Dyspnea/diagnosis ; Dyspnea/etiology ; Humans ; Hypertension, Portal/complications ; Hypertension, Portal/diagnosis ; Liver ; Liver Cirrhosis ; Pancytopenia ; Splenomegaly
    Language English
    Publishing date 2022-04-05
    Publishing country United States
    Document type Case Reports
    ZDB-ID 1032552-9
    ISSN 1931-3543 ; 0012-3692
    ISSN (online) 1931-3543
    ISSN 0012-3692
    DOI 10.1016/j.chest.2021.05.077
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ui III Zs.45: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 349: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top