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  1. Book ; Conference proceedings: Botulinum and tetanus neurotoxins

    Dasgupta, Bibhuti R.

    neurotransmission and biomedical aspects

    1993  

    Event/congress International Conference on Botulinum, Tetanus Neurotoxins - Neurotransmission and Biomedical Aspects (1992, MadisonWis.)
    Author's details ed. by Bibhuti R. DasGupta
    Keywords Botulinum Toxins / pharmacology / congresses ; Tetanus Toxin / pharmacology / congresses ; Neurotoxins / pharmacology / congresses ; Neural Transmission / physiology / congresses ; Botulinustoxin ; Tetanustoxin
    Subject Botulin ; Botulinumtoxin ; Botulinustoxine ; EC 3.4.24.69
    Language English
    Size XVII, 689 S. : Ill., graph. Darst.
    Publisher Plenum Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book ; Conference proceedings
    HBZ-ID HT006129193
    ISBN 0-306-44412-7 ; 978-0-306-44412-8
    Database Catalogue ZB MED Medicine, Health

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  2. Article: Botulinum neurotoxins: perspective on their existence and as polyproteins harboring viral proteases.

    DasGupta, Bibhuti R

    The Journal of general and applied microbiology

    2006  Volume 52, Issue 1, Page(s) 1–8

    MeSH term(s) Botulinum Toxins/chemistry ; Botulinum Toxins/metabolism ; Clostridium botulinum/pathogenicity ; Evolution, Molecular ; Neurotoxins/chemistry ; Neurotoxins/metabolism ; Peptide Hydrolases/chemistry ; Peptide Hydrolases/metabolism ; Polyproteins/chemistry ; Polyproteins/metabolism ; Virulence Factors ; Viruses/enzymology
    Chemical Substances Neurotoxins ; Polyproteins ; Virulence Factors ; Peptide Hydrolases (EC 3.4.-) ; Botulinum Toxins (EC 3.4.24.69)
    Language English
    Publishing date 2006-03-28
    Publishing country Japan
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 218355-9
    ISSN 1349-8037 ; 0022-1260
    ISSN (online) 1349-8037
    ISSN 0022-1260
    DOI 10.2323/jgam.52.1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 996: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Book ; Conference proceedings: Botulinum and tetanus neurotoxins

    DasGupta, Bibhuti R

    neurotransmission and biomedical aspects

    1993  

    Event/congress International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects (1992, MadisonWis.)
    Author's details edited by Bibhuti R. DasGupta
    Keywords Botulinum toxin/Congresses. ; Tetanus toxin/Congresses. ; Neurotoxic agents/Congresses.
    Language English
    Size xvii, 689 p. :, ill. ;, 26 cm.
    Publisher Plenum Press
    Publishing place New York
    Document type Book ; Conference proceedings
    Note "Proceedings of an International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects, held May 11-13, 1992, in Madison, Wisconsin"--T.p. verso.
    ISBN 0306444127 ; 9780306444128
    Database NAL-Catalogue (AGRICOLA)

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  4. Book ; Conference proceedings: Botulinum and tetanus neurotoxins

    DasGupta, Bibhuti R

    neurotransmission and biomedical aspects

    1993  

    Event/congress International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects (1992, MadisonWis.)
    Author's details edited by Bibhuti R. DasGupta
    MeSH term(s) Botulinum Toxins/pharmacology ; Neurotoxins/pharmacology ; Synaptic Transmission/physiology ; Tetanus Toxin/pharmacology
    Language English
    Size xvii, 689 p. :, ill.
    Publisher Plenum Press
    Publishing place New York
    Document type Book ; Conference proceedings
    Note "Proceedings of an International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects, held May 11-13, 1992, in Madison, Wisconsin"--T.p. verso.
    ISBN 9780306444128 ; 0306444127
    Database Catalogue of the US National Library of Medicine (NLM)

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  5. Article ; Online: Partial characterization of a 36-kDa protein from Clostridium botulinum type E that inhibits trypsin and chymotrypsin.

    Prabakaran, Sakthivel / DasGupta, Bibhuti R.

    The Journal of general and applied microbiology

    2002  Volume 45, Issue 4, Page(s) 163–168

    Abstract: A 36-kDa trypsin inhibitor was purified from Clostridium botulinum type E culture supernatant by multiple molecular sieve and ion exchange chromatographic steps. The sequence of the amino-terminal 13 amino acid residues of this single-chain protein is ... ...

    Abstract A 36-kDa trypsin inhibitor was purified from Clostridium botulinum type E culture supernatant by multiple molecular sieve and ion exchange chromatographic steps. The sequence of the amino-terminal 13 amino acid residues of this single-chain protein is Asn.Gln.Glu.Val.Phe.Asn.Met.Pro.Lys.Phe.Ser.Thr.Ala-. This novel protein that also inhibits chymotrypsin is produced by an organism that does not appear to produce any protease.
    Language English
    Publishing date 2002-12-23
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 218355-9
    ISSN 1349-8037 ; 0022-1260
    ISSN (online) 1349-8037
    ISSN 0022-1260
    DOI 10.2323/jgam.45.163
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 996: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Botulinum neurotoxin types A, B, and E: fragmentations by autoproteolysis and other mechanisms including by O-phenanthroline-dithiothreitol, and association of the dinucleotides NAD(+)/NADH with the heavy chain of the three neurotoxins.

    Dasgupta, Bibhuti R / Antharavally, Babu S / Tepp, William / Evenson, Mary L

    The protein journal

    2005  Volume 24, Issue 6, Page(s) 337–368

    Abstract: ... at the amino side of Tyr250 and Arg244, respectively [DasGupta and Foley (1989). Biochimie 71: 1183-1200 ...

    Abstract The first evidence of autoproteolytic activity of the approximately 50-kDa light chain of the clostridial neurotoxins (NT) is traceable to the observations that the light chains of botulinum NT serotypes A and E, separated from their approximately 100-kDa heavy chain conjugate, were found cleaved at the amino side of Tyr250 and Arg244, respectively [DasGupta and Foley (1989). Biochimie 71: 1183-1200]. Specific cleavages of the recombinant light chain of NT type A, including at Tyr249-Tyr250, firmly established that the cleavages reported earlier were due to autoproteolysis [Ahmed et al. (2001). J. Protein Chem. 20: 221-231; Ahmed et al. (2003). Biochemistry 42:12539-12549] and not by contaminating proteases or non-enzymatic. We now report many cleavages in the NT types A, B and E and also in their separated light and heavy chains, and identification of several of the peptide bonds cleaved. None of the identified cleaved bonds (-P1-P1' -) in one serotype (except Asp-Pro) was found common in other serotypes or cleaved within itself at a second site. After separation from the heavy chain self-cleavages of the light chains of type A, B and E at Tyr249-Tyr250, Gln258-Ser259 and Ile243-Arg244, respectively indicate an intriguing feature (in the aligned sequences these bonds of type A and B are 2 and type A and E are 4 peptide bonds apart) that may have some role in the NT's structure-function relationship yet to be understood. We point out that autoproteolysis of a single peptide bond (Phe418-Thr419 or Phe422-Glu423) in NT type A reported by Ahmed et al. (2001) can potentially generate proteolytically active light chain freed of the heavy chain; this is an efficient pathway, that by-passes nicking by a trypsin-like protease(s) inside the intrachain disulfide bridge and its reductive cleavage. We offer probable explanations for the observed cleavages such as acid- and metal-mediated (non-catalytic and non-stoichiometric) reactions in addition to autoproteolysis but cannot predict which mechanism(s) of cleavage occur or prevail following NT's entry in the body as poison or therapeutic agent. The metal chelator O-phenanthroline (above critical miceller concentration) in the presence of dithiothreitol cleaved type E NT at limited sites generating discrete 114-, 87-, 49-, 42-, and 31-kDa fragments but degraded NTs type A and B extensively. The limited cleavage of type E NT was dependent on the presence of metal ion(s) bound to the protein and its native (urea sensitive) conformation. The self-cleavage of the NTs at specific sites prompted us to search for specific binding sites on the NTs analogous to SNARE-motifs-the 9-residuelong motifs present on the NT's natural substrates (SNAP-25, syntaxin, VAMP/synaptobrevin); such putative binding motifs (sites) noted on all clostridial NTs are reported here. Their relationship to the observed autoproteolysis remains to be determined experimentally. The dinucleotide NAD(+)/NADH associated with the NTs type A, B and E (2-3 NADH per protein molecule) via their H-chains, and a portion of the H-chain (toward the C-terminus) appears to exhibit limited amino acid sequence homology with lactate dehydrogenase-a representative NAD(+)/NADH binding protein.
    MeSH term(s) Amino Acid Sequence ; Botulinum Toxins/metabolism ; Botulinum Toxins, Type A/metabolism ; Dithiothreitol ; L-Lactate Dehydrogenase/chemistry ; Molecular Weight ; NAD/metabolism ; Peptide Fragments/chemistry ; Peptide Hydrolases/metabolism ; Phenanthrolines ; Sequence Homology, Amino Acid
    Chemical Substances Peptide Fragments ; Phenanthrolines ; NAD (0U46U6E8UK) ; rimabotulinumtoxinB (0Y70779M1F) ; L-Lactate Dehydrogenase (EC 1.1.1.27) ; Peptide Hydrolases (EC 3.4.-) ; Botulinum Toxins (EC 3.4.24.69) ; Botulinum Toxins, Type A (EC 3.4.24.69) ; botulinum toxin type E (T579M564JY) ; Dithiothreitol (T8ID5YZU6Y) ; 1,10-phenanthroline (W4X6ZO7939)
    Language English
    Publishing date 2005-08
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2143071-8
    ISSN 1875-8355 ; 1573-4943 ; 1572-3887
    ISSN (online) 1875-8355 ; 1573-4943
    ISSN 1572-3887
    DOI 10.1007/s10930-005-7589-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Perceptions of people living with HIV/AIDS

    Pranita Taraphdar / Tapobrata Guha Ray / Dibakar Haldar / Aparajita Dasgupta / Bibhuti Saha

    Indian Journal of Medical Sciences, Vol 64, Iss 10, Pp 441-

    2010  Volume 447

    Abstract: Background: HIV/AIDS being a behavioral disease, appropriate knowledge is important for those who are infected. Objectives: To elicit and compare knowledge and attitude about HIV/AIDS among newly diagnosed and previously diagnosed HIV/AIDS patients ... ...

    Abstract Background: HIV/AIDS being a behavioral disease, appropriate knowledge is important for those who are infected. Objectives: To elicit and compare knowledge and attitude about HIV/AIDS among newly diagnosed and previously diagnosed HIV/AIDS patients attending or admitted in Calcutta School of Tropical Medicine, (CSTM), Kolkata. Materials and Methods: A cross-sectional descriptive study was undertaken among previously diagnosed HIV/AIDS Patients admitted in indoor wards and newly diagnosed HIV/AIDS patients attending Integrated Counseling and Testing Centre (ICTC) of the School of Tropical Medicine, Kolkata. Data were gathered by interviewing patients using a predesigned, pretested, semi-structured questionnaire. Results: More in-patients had heard about AIDS than ICTC patients. Television was the most popular source of information in both groups, followed by health personnel and friends. Correct knowledge about transmission, symptoms, prevention of AIDS, and lifestyles desirable for affected patients was significantly higher among in-patients who had already been counseled, than the newly diagnosed ICTC patients yet to receive. Within each group of patients, the knowledge score was significantly higher among females, Christians, urban residents, patients educated beyond middle school, and non- migrants. In-patients had a significantly higher attitudinal score toward HIV/AIDS. Conclusion: Repeated counseling is required to keep up high level of knowledge and positive attitude pertaining to HIV/AIDS to reduce risk behavior, prevent disease transmission, and improve quality of life.
    Keywords Counseling ; ICTC ; knowledge-attitude ; life-style ; PLWHAs ; Medicine (General) ; R5-920 ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Correlation Between Clinical And Histopathological Criteria For The Classification Of Leprosy

    Mitra Kalyani / Biswas Surajit / Saha Bibhuti / Dasgupta Anjali

    Indian Journal of Dermatology, Vol 46, Iss 3, Pp 135-

    2001  Volume 137

    Abstract: Clinicians and histopathologists sometimes disagree on the classification of a case of leprosy. A detailed histopathological study of 92 fresh uncomplicated cases representing the whole spectrum of leprosy was done for the last one year. There was ... ...

    Abstract Clinicians and histopathologists sometimes disagree on the classification of a case of leprosy. A detailed histopathological study of 92 fresh uncomplicated cases representing the whole spectrum of leprosy was done for the last one year. There was complete agreement between clinical and histopathological diagnosis in 53 cases (57.61)% and disparity in 39 cases (42.39%). Among the latter, shift towards the lepromatous pole of the spectrum was found in 13 cases (14.13%) and shift towards tuberculoid pole in 26 cases (28.26%). The latter group included 16 cases (17.39%) where histopathological diagnosis was indeterminate leprosy because of the presence of early histopathological changes. It is concluded that in case of discrepancy, the more advanced finding (i.e. towards the lepromatous pole) should be given greater weightage and the case is to be classified and treated accordingly.
    Keywords Dermatology ; RL1-803 ; Medicine ; R ; DOAJ:Dermatology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2001-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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