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Article ; Online: Prospective Evaluation of the BD MAX StaphSR Assay for the Screening of Methicillin-Susceptible and -Resistant

Fayolle, Martin / Epercieux, Amélie / Haddar, Cyrille H / Pillet, Sylvie / Berthelot, Philippe / Pozzetto, Bruno / Carricajo, Anne / Grattard, Florence / Verhoeven, Paul O

International journal of molecular sciences

2023 Volume 24, Issue 18

Abstract: Screening patients ... ...

Abstract	Screening patients for
MeSH term(s)	Humans ; Staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus ; Methicillin ; Prospective Studies ; Staphylococcal Infections/diagnosis ; Staphylococcal Infections/microbiology ; Sensitivity and Specificity ; Intensive Care Units
Chemical Substances	Methicillin (Q91FH1328A)
Language	English
Publishing date	2023-09-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241813881
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.

Haddar, Cyrille / Verhoeven, Paul O / Bourlet, Thomas / Pozzetto, Bruno / Pillet, Sylvie

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

2020 Volume 132, Page(s) 104636

Abstract: Background: Facing the emergence of a new RNA virus, clinical laboratories are often helpless in the case of a shortage of reagents recommended by Reference Centres.: Objectives: To compare five open one step RT-qPCR reagents to the SuperScript™ III ... ...

Abstract	Background: Facing the emergence of a new RNA virus, clinical laboratories are often helpless in the case of a shortage of reagents recommended by Reference Centres. Objectives: To compare five open one step RT-qPCR reagents to the SuperScript™ III Platinum™ One-Step qRT-PCR kit (Invitrogen) considered as the reference one in France at the beginning of the pandemic for detection of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in respiratory specimens by using a laboratory-developed assay targeting the viral RNA dependant RNA polymerase (RdRp) gene. Study design: A total of 51 NUCLISENS easyMAG extracts from respiratory specimens was tested on ABI 7500 thermocycler with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs), GoTaq® Probe 1- Step RT-qPCR System (Promega), LightCycler® Multiplex RNA Virus Master (Roche) and One-step PrimeScript RT-PCR kit (Takara). The CT values obtained using the 5 challenged reagents were compared to those obtained using the reference assay. Results: The percentages of concordance were all above 95 %. When comparing the CT values of the 48 extracts exhibiting CT values < 35 obtained with the reference reagent, the results were similar between the reagents although the differences of CT values were quite dispersed. Conclusions: All five reagents can be considered as alternative reagents to the reference for detecting SARS-CoV-2 RNA.
MeSH term(s)	COVID-19/diagnosis ; Humans ; Nasopharynx/virology ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Sputum/virology ; Trachea/virology
Chemical Substances	RNA, Viral
Keywords	covid19
Language	English
Publishing date	2020-09-08
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2020.104636
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Article ; Online: Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe

Haddar, Cyrille / Verhoeven, Paul O. / Bourlet, Thomas / Pozzetto, Bruno / Pillet, Sylvie

Journal of Clinical Virology

2020 Volume 132, Page(s) 104636

Keywords	Virology ; Infectious Diseases ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2020.104636
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Article: Effect of a lotion containing the heat-treated probiotic strain

Blanchet-Réthoré, Sandrine / Bourdès, Valérie / Mercenier, Annick / Haddar, Cyrille H / Verhoeven, Paul O / Andres, Philippe

Clinical, cosmetic and investigational dermatology

2017 Volume 10, Page(s) 249–257

Abstract: Objective: Staphylococcus aureus: Methods: This open-label, multicenter study was performed in AD patients in Germany. First, detection of : Results: Thirty-one patients with AD were included in the study. All sampling and quantification methods ... ...

Abstract	Objective: Staphylococcus aureus Methods: This open-label, multicenter study was performed in AD patients in Germany. First, detection of Results: Thirty-one patients with AD were included in the study. All sampling and quantification methods were found to be robust, reproducible, and repeatable for assessing Conclusion: This study demonstrated that the application of a lotion containing HT La1 to the lesional skin of patients with AD for 3 weeks controlled
Language	English
Publishing date	2017-07-03
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2494852-4
ISSN	1178-7015
ISSN	1178-7015
DOI	10.2147/CCID.S135529
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Article: Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe

Haddar, Cyrille / Verhoeven, Paul O / Bourlet, Thomas / Pozzetto, Bruno / Pillet, Sylvie

J Clin Virol

Abstract: BACKGROUND: Facing the emergence of a new RNA virus, clinical laboratories are often helpless in the case of a shortage of reagents recommended by Reference Centres. OBJECTIVES: To compare five open one step RT-qPCR reagents to the SuperScript™ III ... ...

Abstract	BACKGROUND: Facing the emergence of a new RNA virus, clinical laboratories are often helpless in the case of a shortage of reagents recommended by Reference Centres. OBJECTIVES: To compare five open one step RT-qPCR reagents to the SuperScript™ III Platinum™ One-Step qRT-PCR kit (Invitrogen) considered as the reference one in France at the beginning of the pandemic for detection of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in respiratory specimens by using a laboratory-developed assay targeting the viral RNA dependant RNA polymerase (RdRp) gene. STUDY DESIGN: A total of 51 NUCLISENS easyMAG extracts from respiratory specimens was tested on ABI 7500 thermocycler with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs), GoTaq® Probe 1- Step RT-qPCR System (Promega), LightCycler® Multiplex RNA Virus Master (Roche) and One-step PrimeScript RT-PCR kit (Takara). The CT values obtained using the 5 challenged reagents were compared to those obtained using the reference assay. RESULTS: The percentages of concordance were all above 95 %. When comparing the CT values of the 48 extracts exhibiting CT values < 35 obtained with the reference reagent, the results were similar between the reagents although the differences of CT values were quite dispersed. CONCLUSIONS: All five reagents can be considered as alternative reagents to the reference for detecting SARS-CoV-2 RNA.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #747685
Database	COVID19

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Article ; Online: Comparison of the Fully Automated FilmArray BCID Assay to a 4-Hour Culture Test Coupled to Mass Spectrometry for Day 0 Identification of Microorganisms in Positive Blood Cultures.

Verhoeven, Paul O / Haddar, Cyrille H / Rigaill, Josselin / Fonsale, Nathalie / Carricajo, Anne / Grattard, Florence / Pozzetto, Bruno

BioMed research international

2018 Volume 2018, Page(s) 7013470

Abstract: Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture ... ...

Abstract	Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture Identification (BCID) assay by comparison to an in-house protocol based on MALDI-TOF MS identification of microcolonies after a 4-hour culture, for identifying on the same day the microorganisms present in positive blood culture bottles. One hundred and fifty-three positive bottles from 123 patients were tested prospectively by the 3 techniques of bacterial identification: 11 bottles yielding negative results by the 3 tests were considered false positive (7.2%). The reference MALDI-TOF MS technique identified 134 monomicrobial (87.6%) and 8 double infections (5.2%), which resulted in a total of 150 microorganisms. Globally, 137 (91.3%) of these 150 pathogens were correctly identified by the fully automated multiplex FilmArray BCID system at the species or genus level on day of growth detection, versus 117 (78.8%) by MALDI-TOF MS identification on nascent microcolonies after a 4-hour culture (
MeSH term(s)	Anti-Infective Agents/pharmacology ; Bacteria/drug effects ; Biological Assay/methods ; Blood Culture/methods ; Humans ; Molecular Diagnostic Techniques/methods ; Prospective Studies ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemical Substances	Anti-Infective Agents
Language	English
Publishing date	2018-11-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2018/7013470
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Article ; Online: Strategy using a new antigenic test for rapid diagnosis of Streptococcus pneumoniae infection in respiratory samples from children consulting at hospital.

Haddar, Cyrille H / Joly, Johan / Carricajo, Anne / Verhoeven, Paul O / Grattard, Florence / Mory, Olivier / Begaud, Evelyne / Germani, Yves / Cantais, Aymeric / Pozzetto, Bruno

BMC microbiology

2020 Volume 20, Issue 1, Page(s) 79

Abstract: Background: Despite vaccination programs, Streptococcus pneumoniae remains among the main microorganisms involved in bacterial pneumonia, notably in terms of severity. The prognosis of pneumococcal infections is conditioned in part by the precocity of ... ...

Abstract	Background: Despite vaccination programs, Streptococcus pneumoniae remains among the main microorganisms involved in bacterial pneumonia, notably in terms of severity. The prognosis of pneumococcal infections is conditioned in part by the precocity of the diagnosis. The aim of this study was to evaluate the impact of a Rapid Diagnostic Test (RDT) targeting cell wall polysaccharide of Streptococcus pneumoniae and performed directly in respiratory samples, on the strategy of diagnosis of respiratory pneumococcal infections in children. Results: Upper-respiratory tract samples from 196 children consulting at hospital for respiratory infection were tested for detecting S. pneumoniae using a newly-designed RDT (PneumoResp, Biospeedia), a semi-quantitative culture and two PCR assays. If positive on fluidized undiluted specimen, the RDT was repeated on 1:100-diluted sample. The RDT was found highly specific when tested on non-S. pneumoniae strains. By comparison to culture and PCR assays, the RDT on undiluted secretions exhibited a sensitivity (Se) and negative predictive value (NPV) of more than 98%. By comparison to criteria of S. pneumoniae pneumonia combining typical symptoms, X-ray image, and culture ≥10 Conclusions: In case of negative result, the excellent NPV of RDT on undiluted secretions allows excluding S. pneumoniae pneumonia. In case of positive result, the excellent sensitivity of RDT on diluted secretions for the diagnosis of S. pneumoniae pneumonia allows proposing a suitable antimicrobial treatment at day 0.
MeSH term(s)	Adolescent ; Antigens, Bacterial/genetics ; Child ; Child, Preschool ; Early Diagnosis ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Microbiological Techniques/methods ; Pneumococcal Infections/diagnosis ; Pneumococcal Infections/immunology ; Polysaccharides, Bacterial/genetics ; Polysaccharides, Bacterial/immunology ; Prognosis ; Respiratory Tract Infections/microbiology ; Sensitivity and Specificity ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/growth & development ; Streptococcus pneumoniae/immunology ; Streptococcus pneumoniae/isolation & purification
Chemical Substances	Antigens, Bacterial ; Polysaccharides, Bacterial
Language	English
Publishing date	2020-04-07
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1471-2180
ISSN (online)	1471-2180
DOI	10.1186/s12866-020-01764-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Validation of a New Rapid Detection Test for Detection of Neisseria meningitidis A/C/W/X/Y Antigens in Cerebrospinal Fluid.

Haddar, Cyrille H / Terrade, Aude / Verhoeven, Paul / Njanpop-Lafourcade, Berthe-Marie / Dosso, Mireille / Sidikou, Fati / Mahamane, Ali Elhaj / Lombart, Jean-Pierre / Razki, Aziza / Hong, Eva / Agnememel, Alain / Begaud, Evelyne / Germani, Yves / Pozzetto, Bruno / Taha, Muhamed-Kheir

Journal of clinical microbiology

2020 Volume 58, Issue 3

Abstract: Meningococcal meningitis remains a life-threatening disease worldwide, with high prevalence in the sub-Saharan meningitis belt. A rapid diagnosis is crucial for implementing adapted antimicrobial treatment. We describe the performances of a new ... ...

Abstract	Meningococcal meningitis remains a life-threatening disease worldwide, with high prevalence in the sub-Saharan meningitis belt. A rapid diagnosis is crucial for implementing adapted antimicrobial treatment. We describe the performances of a new immunochromatographic test (MeningoSpeed, BioSpeedia, France) for detecting and grouping
MeSH term(s)	Africa ; Antigens, Bacterial ; Cerebrospinal Fluid ; France ; Humans ; Meningitis, Meningococcal/diagnosis ; Neisseria meningitidis/genetics ; Sensitivity and Specificity
Chemical Substances	Antigens, Bacterial
Language	English
Publishing date	2020-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01699-19
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Article ; Online: Comparison of the Fully Automated FilmArray BCID Assay to a 4-Hour Culture Test Coupled to Mass Spectrometry for Day 0 Identification of Microorganisms in Positive Blood Cultures

Paul O. Verhoeven / Cyrille H. Haddar / Josselin Rigaill / Nathalie Fonsale / Anne Carricajo / Florence Grattard / Bruno Pozzetto

BioMed Research International, Vol

2018 Volume 2018

Abstract	Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture Identification (BCID) assay by comparison to an in-house protocol based on MALDI-TOF MS identification of microcolonies after a 4-hour culture, for identifying on the same day the microorganisms present in positive blood culture bottles. One hundred and fifty-three positive bottles from 123 patients were tested prospectively by the 3 techniques of bacterial identification: 11 bottles yielding negative results by the 3 tests were considered false positive (7.2%). The reference MALDI-TOF MS technique identified 134 monomicrobial (87.6%) and 8 double infections (5.2%), which resulted in a total of 150 microorganisms. Globally, 137 (91.3%) of these 150 pathogens were correctly identified by the fully automated multiplex FilmArray BCID system at the species or genus level on day of growth detection, versus 117 (78.8%) by MALDI-TOF MS identification on nascent microcolonies after a 4-hour culture (P < 0.01). By combining the two approaches, 140 (93.5%) of the positive bottles were identified successfully at day 0. These results confirm the excellent sensitivity of the FilmArray BCID assay, notably in case of multimicrobial infection. Due to the limited number of targets included into the test, it must be coupled to another identification strategy, as that presented in this study relying on MALDI-TOF MS identification of microcolonies obtained after a very short culture period.
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Does pharyngeal sampling improve the detection of nasopharyngeal persistent carriers of Staphylococcus aureus?

Verhoeven, Paul O / Haddar, Cyrille H / Grattard, Florence / Carricajo, Anne / Botelho-Nevers, Elisabeth / Pozzetto, Bruno / Berthelot, Philippe

The Journal of infection

2015 Volume 70, Issue 5, Page(s) 549–552

MeSH term(s)	Carrier State/diagnosis ; Carrier State/microbiology ; Humans ; Nasopharynx/microbiology ; Pharynx/microbiology ; Staphylococcal Infections/diagnosis ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/isolation & purification
Language	English
Publishing date	2015-05
Publishing country	England
Document type	Letter
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2015.01.003
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