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Article: Metagenomic analysis of bacterial communities of Wadi Namar Lake, Riyadh, Saudi Arabia.

Alotaibi, Modhi O / Mohammed, Afrah E / Eltom, Kamal H

2022 Volume 29, Issue 5, Page(s) 3749–3758

Abstract: Wadi Namar lake is a new touristic attraction area in the south of Riyadh. Human activities around the lake may lead to changes in water quality with subsequent changes in microenvironment components including microbial diversity. The current study was ... ...

Abstract	Wadi Namar lake is a new touristic attraction area in the south of Riyadh. Human activities around the lake may lead to changes in water quality with subsequent changes in microenvironment components including microbial diversity. The current study was designed to assess possible changes in bacterial communities of the water at Wadi Namar Lake. Therefore, water samples were collected from three different locations along the lake: L1 (no human activities, no plants), L2 (no human activity, some plants) and L3 (human activities, municipal wastes and some plants). The total DNA of the samples was extracted and subjected to 16S rDNA sequencing and metagenomic analysis; water pH, electrical conductivity (EC), total dissolved solids (TDS) as well as the concentration of Na
Language	English
Publishing date	2022-03-06
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2022.03.001
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Article ; Online: Metagenomic analysis of bacterial communities of Wadi Namar Lake, Riyadh, Saudi Arabia

Modhi O. Alotaibi / Afrah E. Mohammed / Kamal H. Eltom

Saudi Journal of Biological Sciences, Vol 29, Iss 5, Pp 3749-

2022 Volume 3758

Abstract	Wadi Namar lake is a new touristic attraction area in the south of Riyadh. Human activities around the lake may lead to changes in water quality with subsequent changes in microenvironment components including microbial diversity. The current study was designed to assess possible changes in bacterial communities of the water at Wadi Namar Lake. Therefore, water samples were collected from three different locations along the lake: L1 (no human activities, no plants), L2 (no human activity, some plants) and L3 (human activities, municipal wastes and some plants). The total DNA of the samples was extracted and subjected to 16S rDNA sequencing and metagenomic analysis; water pH, electrical conductivity (EC), total dissolved solids (TDS) as well as the concentration of Na+1, K+1, Cl−1 and total N were analysed. Metagenomic analysis showed variations in relative abundance of 17 phyla, 31 families, 43 genera and 19 species of bacteria between the locations. Proteobacteria was the most abundant phylum in all locations; however, its highest abundance was in L1. Planctomycete phylum was highly abundant in L1 and L3, while its abundance in L2 was low. The phyla Acidobacteria, Candidatus Saccharibacteria, Nitrospirae and Chloroflexi were associated with high TDS, EC, K+1 and Cl−1 concentrations in L3; various human activities around this location had possibly affected microbial diversity. Current study results help in recognising the structure of bacterial communities at Wadi Namar Lake in relation to their surroundings for planning to environment protection and future restoration of affected ecosystems.
Keywords	16S rRNA gene ; Bacterial abundance ; Metagenomics ; Water microbiome ; Environment ; Biology (General) ; QH301-705.5
Subject code	580
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Mycobacterium avium

Ssekitoleko, Judah / Ojok, Lonzy / Abd El Wahed, Ahmed / Erume, Joseph / Amanzada, Ahmad / Eltayeb, ElSagad / Eltom, Kamal H / Okuni, Julius Boniface

Microorganisms

2021 Volume 9, Issue 12

Abstract: To propose a solution for control ... ...

Abstract	To propose a solution for control of
Language	English
Publishing date	2021-12-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9122623
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Article: Buffalopox Virus: An Emerging Virus in Livestock and Humans.

Eltom, Kamal H / Samy, Abdallah M / Abd El Wahed, Ahmed / Czerny, Claus-Peter

Pathogens (Basel, Switzerland)

2020 Volume 9, Issue 9

Abstract: Buffalopox virus (BPXV) is the cause of buffalopox, which was recognized by the FAO/WHO Joint Expert Committee on Zoonosis as an important zoonotic disease. Buffalopox was first described in India, later in other countries, and has become an emerging ... ...

Abstract	Buffalopox virus (BPXV) is the cause of buffalopox, which was recognized by the FAO/WHO Joint Expert Committee on Zoonosis as an important zoonotic disease. Buffalopox was first described in India, later in other countries, and has become an emerging contagious viral zoonotic disease infecting milkers with high morbidity among affected domestic buffalo and cattle. BPXV is a member of the genus
Keywords	covid19
Language	English
Publishing date	2020-08-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens9090676
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Article: Buffalopox Virus: An Emerging Virus in Livestock and Humans

Eltom, Kamal H / Samy, Abdallah M / Abd El Wahed, Ahmed / Czerny, Claus-Peter

Pathogens. 2020 Aug. 20, v. 9, no. 9

2020

Abstract	Buffalopox virus (BPXV) is the cause of buffalopox, which was recognized by the FAO/WHO Joint Expert Committee on Zoonosis as an important zoonotic disease. Buffalopox was first described in India, later in other countries, and has become an emerging contagious viral zoonotic disease infecting milkers with high morbidity among affected domestic buffalo and cattle. BPXV is a member of the genus Orthopoxvirus and a close variant of the vaccinia virus (VACV). Recent genome data show that BPXV shares a most recent common ancestor of VACV Lister strain, which had been used for inoculating buffalo calves to produce a Smallpox vaccine. Over time, VACV evolved into BPXV by establishing itself in buffaloes to be increasingly pathogenic to this host and to make infections in cattle and humans. Together with the current pandemic of SARS-COV2/COVID 19, BPXV infections illustrate how vulnerable the human population is to the emergence and re-emergence of viral pathogens from unsuspected sources. In view that majority of the world population are not vaccinated against smallpox and are most vulnerable in the event of its re-emergence, reviewing and understanding the biology of vaccinia-like viruses are necessary for developing a new generation of safer smallpox vaccines in the smallpox-free world.
Keywords	Vaccinia virus ; ancestry ; buffaloes ; cattle ; genome ; human population ; humans ; morbidity ; pandemic ; pathogens ; population ; smallpox ; vaccines ; viruses ; zoonoses ; India
Language	English
Dates of publication	2020-0820
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens9090676
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Evaluation of antibiotic-induced behavioral changes in mice.

Kwon, Hye-Joo / Mohammed, Afrah E / Eltom, Kamal H / Albrahim, Jehan S / Alburae, Najla Ali

Physiology & behavior

2020 Volume 223, Page(s) 113015

Abstract: Gut microbiota (GM) plays a critical role in health maintenance. Previous reports connected GM with metabolic, immunologic and neurologic pathways. The main purpose of the current investigation was to study whether antibiotic-induced disturbances of GM ... ...

Abstract	Gut microbiota (GM) plays a critical role in health maintenance. Previous reports connected GM with metabolic, immunologic and neurologic pathways. The main purpose of the current investigation was to study whether antibiotic-induced disturbances of GM affects psychological or behavioral conditions on mice as animal model. Mice were exposed to clindamycin or amoxicillin, and their behaviors were evaluated. Antibiotic-treated groups displayed reduced recognition memory and increased depression. No significant changes in the locomotor activity and anxiety were observed. Our data suggested that changes in GM composition by antibiotics may lead to the cognitive and behavioral deficit.
MeSH term(s)	Animals ; Anti-Bacterial Agents/toxicity ; Anxiety/chemically induced ; Behavior, Animal ; Depression/chemically induced ; Gastrointestinal Microbiome ; Mice
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2020-06-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3907-x
ISSN	1873-507X ; 0031-9384
ISSN (online)	1873-507X
ISSN	0031-9384
DOI	10.1016/j.physbeh.2020.113015
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Article: First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques.

Idris, Sanaa M / Ali, Eva A / Elmagzoub, Wisal A / Okuni, Julius B / Mukhtar, Mohamed E / Ojok, Lonzy / Eltayeb, ElSagad / Abd El Wahed, Ahmed / Eltom, Kamal H / Gameel, Ahmed A

Animals : an open access journal from MDPI

2022 Volume 12, Issue 23

Abstract: Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of ... ...

Abstract	Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and faecal samples were collected from all animals (N = 59): harvested sera were tested for anti-
Language	English
Publishing date	2022-11-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12233312
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Article ; Online: Buffalopox Virus

Kamal H. Eltom / Abdallah M. Samy / Ahmed Abd El Wahed / Claus-Peter Czerny

Pathogens, Vol 9, Iss 676, p

An Emerging Virus in Livestock and Humans

2020 Volume 676

Abstract	Buffalopox virus (BPXV) is the cause of buffalopox, which was recognized by the FAO/WHO Joint Expert Committee on Zoonosis as an important zoonotic disease. Buffalopox was first described in India, later in other countries, and has become an emerging contagious viral zoonotic disease infecting milkers with high morbidity among affected domestic buffalo and cattle. BPXV is a member of the genus Orthopoxvirus and a close variant of the vaccinia virus (VACV). Recent genome data show that BPXV shares a most recent common ancestor of VACV Lister strain, which had been used for inoculating buffalo calves to produce a Smallpox vaccine. Over time, VACV evolved into BPXV by establishing itself in buffaloes to be increasingly pathogenic to this host and to make infections in cattle and humans. Together with the current pandemic of SARS-COV2/COVID 19, BPXV infections illustrate how vulnerable the human population is to the emergence and re-emergence of viral pathogens from unsuspected sources. In view that majority of the world population are not vaccinated against smallpox and are most vulnerable in the event of its re-emergence, reviewing and understanding the biology of vaccinia-like viruses are necessary for developing a new generation of safer smallpox vaccines in the smallpox-free world.
Keywords	buffalopox ; VACV ; smallpox ; orthopoxviruses ; Medicine ; R ; covid19
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular and serological survey of paratuberculosis in cattle in selected districts of Western Uganda.

Ssekitoleko, Judah / Ojok, Lonzy / Omala, Saint Kizito / Mukhtar, Mohammed Elwasila / Eltom, Kamal H / Eltayeb, El Sagad / Kankya, Clovice / Kisekka, Magid / Truyen, Uwe / Czerny, Claus-Peter / El Wahed, Ahmed Abd / Okuni, Julius Boniface

BMC veterinary research

2022 Volume 18, Issue 1, Page(s) 438

Abstract: Knowledge of Mycobacterium avium subsp. paratuberculosis (MAP) herd infection status is important to plan appropriate control and prevention strategies for Paratuberculosis (PTB); however, in Uganda MAP infection status of most herds is unknown. This ... ...

Abstract	Knowledge of Mycobacterium avium subsp. paratuberculosis (MAP) herd infection status is important to plan appropriate control and prevention strategies for Paratuberculosis (PTB); however, in Uganda MAP infection status of most herds is unknown. This study aimed at determining the MAP infection status of cattle herds and the associated risk factors for MAP infection in six western districts of Uganda. The survey covered a total of 93 herds where faecal and blood samples were collected from 1814 cattle. A Recombinase Polymerase Amplification (RPA) and an antibody-based (ELISA) assays were used to test for the presence of MAP DNA in faeces and MAP antibodies in serum, respectively. The apparent cow-level prevalence of MAP infection was 3.2 and 2.7% using ELISA and RPA respectively and the true cow-level prevalence using ELISA and RPA was 4.9 and 3% respectively. A herd-level prevalence of 43% (ELISA) and 40.8% (RPA) and a within-herd prevalence of 3.8 ± 2.1% based on ELISA were obtained. Among the risk factors investigated, long dry spells were significantly associated with high MAP infection (p < 0.05). These results indicate that MAP is actively present in most areas where surveillance was carried out. This poses a serious threat to the livestock industry and potentially to public health as MAP is highly suspected to play a role in the pathogenesis of several diseases in humans. Other areas of the country are to be surveyed as well in order to establish full data on MAP infection status to enable interventions for the control and prevention of the disease.
MeSH term(s)	Female ; Humans ; Cattle ; Animals ; Paratuberculosis/epidemiology ; Paratuberculosis/microbiology ; Uganda/epidemiology ; Cattle Diseases/epidemiology ; Cattle Diseases/microbiology ; Mycobacterium avium subsp. paratuberculosis ; Enzyme-Linked Immunosorbent Assay/veterinary ; Feces/microbiology ; Prevalence ; Dairying
Language	English
Publishing date	2022-12-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2191675-5
ISSN	1746-6148 ; 1746-6148
ISSN (online)	1746-6148
ISSN	1746-6148
DOI	10.1186/s12917-022-03535-7
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Article: Paratuberculosis: The Hidden Killer of Small Ruminants.

Idris, Sanaa M / Eltom, Kamal H / Okuni, Julius B / Ojok, Lonzy / Elmagzoub, Wisal A / El Wahed, Ahmed Abd / Eltayeb, ElSagad / Gameel, Ahmed A

Animals : an open access journal from MDPI

2021 Volume 12, Issue 1

Abstract: Paratuberculosis (PTB) is a contagious and chronic enteric disease of ruminants and many non-ruminants caused ... ...

Abstract	Paratuberculosis (PTB) is a contagious and chronic enteric disease of ruminants and many non-ruminants caused by
Language	English
Publishing date	2021-12-21
Publishing country	Switzerland
Document type	Journal Article ; Review
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12010012
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