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Article ; Online: Community Service as a Criminal Sanction in Serbian Criminal Legislation.

Ćorović, Emir / Turanjanin, Veljko / Spasić, Dragana

International journal of offender therapy and comparative criminology

2022 , Page(s) 306624X221132993

Abstract: This paper analyses the normative framework governing the punishment of community service in Serbian criminal legislation. In this legislation, community service is prescribed as a criminal sanction. Accordingly, the paper has analyzed the provisions of ... ...

Abstract	This paper analyses the normative framework governing the punishment of community service in Serbian criminal legislation. In this legislation, community service is prescribed as a criminal sanction. Accordingly, the paper has analyzed the provisions of every law that regulates this criminal sanction as well as statistics. A good normative framework is a precondition for the proper implementation of all legal institutes, including community service. Comprehensive analysis of the substantive, procedural, and executive regulations related to the punishment in question, seeks to identify certain failures in its regulations, as well as to give certain suggestions
Language	English
Publishing date	2022-11-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	218274-9
ISSN	1552-6933 ; 0306-624X
ISSN (online)	1552-6933
ISSN	0306-624X
DOI	10.1177/0306624X221132993
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Article ; Online: Allantoic Fluid-Based qPCR for Early Onset

Monteiro Belo Santos, Simão / Corion, Matthias / De Ketelaere, Bart / Lammertyn, Jeroen / Spasic, Dragana

Journal of agricultural and food chemistry

2024

Abstract: The day-old male chick culling remains a welfare issue in the poultry industry. Several governments have prohibited this practice, pushing hatcheries to seek alternatives. Although different solutions exist for solving this problem, sex determination ... ...

Abstract	The day-old male chick culling remains a welfare issue in the poultry industry. Several governments have prohibited this practice, pushing hatcheries to seek alternatives. Although different solutions exist for solving this problem, sex determination during the embryo's incubation (
Language	English
Publishing date	2024-04-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c09418
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Article ; Online: Monitoring the Coagulation Profile of COVID-19 Patients Using Standard and ClotPro

Milić, Dragan / Lazarević, Milan / Vuković, Natalija / Kamenov, Aleksandar / Perić, Velimir / Golubović, Mlađan / Stošić, Marija / Spasić, Dimitrije / Stojiljković, Vladimir / Stokanović, Dragana

Medicina (Kaunas, Lithuania)

2023 Volume 59, Issue 7

Abstract: Background and ... ...

Abstract	Background and Objectives
MeSH term(s)	Humans ; Thrombelastography/adverse effects ; Thrombelastography/methods ; COVID-19/complications ; Hemostasis ; Blood Coagulation Disorders/etiology ; Thrombosis/diagnosis ; Thrombosis/etiology ; Fibrinogen ; Inflammation/complications ; Peptide Fragments ; Lipopolysaccharide Receptors
Chemical Substances	antineoplaston A10 (16VY3TM7ZO) ; Fibrinogen (9001-32-5) ; presepsin protein, human ; Peptide Fragments ; Lipopolysaccharide Receptors
Language	English
Publishing date	2023-06-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2188113-3
ISSN	1648-9144 ; 1010-660X
ISSN (online)	1648-9144
ISSN	1010-660X
DOI	10.3390/medicina59071202
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Article ; Online: Imaging the unimaginable: leveraging signal generation of CRISPR-Cas for sensitive genome imaging.

Van Tricht, Charlotte / Voet, Thierry / Lammertyn, Jeroen / Spasic, Dragana

Trends in biotechnology

2022 Volume 41, Issue 6, Page(s) 769–784

Abstract: Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with RNA imaging is challenging. Consequently, due to its capacity ... ...

Abstract	Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with RNA imaging is challenging. Consequently, due to its capacity to bind double-stranded DNA (dsDNA) and design flexibility, the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (CRISPR-Cas9) technology has sparked enormous interest over the past decade. In this review, we describe various nucleic acid (NA)- and protein-based (amplified) signal generation methods that achieve imaging of repetitive and single-copy sequences, and even single-nucleotide variants (SNVs), next to highly multiplexed as well as dynamic imaging in live cells. With future progress in the field, the CRISPR-(d)Cas9-based technology promises to break through as a next-generation cell-imaging technique.
MeSH term(s)	CRISPR-Cas Systems ; In Situ Hybridization, Fluorescence ; Genome ; DNA/genetics ; DNA/metabolism ; Diagnostic Imaging
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2022-11-08
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	47474-5
ISSN	1879-3096 ; 0167-7799
ISSN (online)	1879-3096
ISSN	0167-7799
DOI	10.1016/j.tibtech.2022.10.003
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Article: Trends in in ovo sexing technologies: insights and interpretation from papers and patents.

Corion, Matthias / Santos, Simão / De Ketelaere, Bart / Spasic, Dragana / Hertog, Maarten / Lammertyn, Jeroen

Journal of animal science and biotechnology

2023 Volume 14, Issue 1, Page(s) 102

Abstract: Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception. This review gives a complete overview of ...

Abstract	Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception. This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat/Espacenet databases for papers and patents, respectively. Based on a total of 49 papers and 115 patent families reported until May 2023 worldwide, 11 technology categories were defined: 6 non-optical and 5 optical techniques. Every category was described for its characteristics while assessing its potential for application. Next, the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves, and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents. When comparing the reported technologies in papers to those in patents, scientific gaps were observed, as some of the patented technologies were not reported in the scientific literature, e.g., ion mobility and mass spectrometry approaches. Generally, more diverse approaches in all categories were found in patents, although they do require more scientific evidence through papers or industrial adoption to prove their robustness. Moreover, although there is a recent trend for non-invasive techniques, invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported (and might continue to be) in papers and patents. It was also observed that none of the technologies complies with all the industry requirements, although 5 companies already entered the market. On the one hand, more research and harmony between consumers, industry, and governments is necessary. On the other hand, close monitoring of the market performance of the currently available techniques will offer valuable insights into the potential and expectations of in ovo sexing techniques in the poultry industry.
Language	English
Publishing date	2023-07-14
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2630162-3
ISSN	2049-1891 ; 1674-9782
ISSN (online)	2049-1891
ISSN	1674-9782
DOI	10.1186/s40104-023-00898-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Unraveling the Kinetics of the 10-23 RNA-Cleaving DNAzyme.

Montserrat Pagès, Aida / Hertog, Maarten / Nicolaï, Bart / Spasic, Dragana / Lammertyn, Jeroen

International journal of molecular sciences

2023 Volume 24, Issue 18

Abstract: DNA-based enzymes, or DNAzymes, are single-stranded DNA sequences with the ability to catalyze various chemical reactions, including the cleavage of the bond between two RNA nucleotides. Lately, an increasing interest has been observed in these RNA- ... ...

Abstract	DNA-based enzymes, or DNAzymes, are single-stranded DNA sequences with the ability to catalyze various chemical reactions, including the cleavage of the bond between two RNA nucleotides. Lately, an increasing interest has been observed in these RNA-cleaving DNAzymes in the biosensing and therapeutic fields for signal generation and the modulation of gene expression, respectively. Additionally, multiple efforts have been made to study the effects of the reaction environment and the sequence of the catalytic core on the conversion of the substrate into product. However, most of these studies have only reported alterations of the general reaction course, but only a few have focused on how each individual reaction step is affected. In this work, we present for the first time a mathematical model that describes and predicts the reaction of the 10-23 RNA-cleaving DNAzyme. Furthermore, the model has been employed to study the effect of temperature, magnesium cations and shorter substrate-binding arms of the DNAzyme on the different kinetic rate constants, broadening the range of conditions in which the model can be exploited. In conclusion, this work depicts the prospects of such mathematical models to study and anticipate the course of a reaction given a particular environment.
MeSH term(s)	DNA, Catalytic ; Catalysis ; Catalytic Domain ; DNA, Single-Stranded/genetics ; RNA/genetics
Chemical Substances	DNA, Catalytic ; DNA, Single-Stranded ; RNA (63231-63-0)
Language	English
Publishing date	2023-09-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241813686
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Article ; Online: Trends in in ovo sexing technologies

Matthias Corion / Simão Santos / Bart De Ketelaere / Dragana Spasic / Maarten Hertog / Jeroen Lammertyn

Journal of Animal Science and Biotechnology, Vol 14, Iss 1, Pp 1-

insights and interpretation from papers and patents

2023 Volume 24

Abstract: Abstract Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception. This review gives a complete ... ...

Abstract	Abstract Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception. This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat/Espacenet databases for papers and patents, respectively. Based on a total of 49 papers and 115 patent families reported until May 2023 worldwide, 11 technology categories were defined: 6 non-optical and 5 optical techniques. Every category was described for its characteristics while assessing its potential for application. Next, the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves, and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents. When comparing the reported technologies in papers to those in patents, scientific gaps were observed, as some of the patented technologies were not reported in the scientific literature, e.g., ion mobility and mass spectrometry approaches. Generally, more diverse approaches in all categories were found in patents, although they do require more scientific evidence through papers or industrial adoption to prove their robustness. Moreover, although there is a recent trend for non-invasive techniques, invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported (and might continue to be) in papers and patents. It was also observed that none of the technologies complies with all the industry requirements, although 5 companies already entered the market. On the one hand, more research and harmony between consumers, industry, and governments is necessary. On the other hand, close monitoring of the market performance of the currently available techniques will offer valuable insights into the potential and ...
Keywords	Animal welfare ; In ovo sexing ; Male day-old chick culling ; Optical and non-optical techniques ; Patents and papers ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
Subject code	001
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?

Safdar, Saba / Lammertyn, Jeroen / Spasic, Dragana

Trends in biotechnology

2020 Volume 38, Issue 12, Page(s) 1343–1359

Abstract: Nucleic acid enzymes (NAzymes) are nucleic acid molecules with catalytic activity. A subset, the RNA-cleaving NAzyme, is characterized by its substrate of choice: an RNA unit. These enzymes have been used for diverse applications, including biosensor ... ...

Abstract	Nucleic acid enzymes (NAzymes) are nucleic acid molecules with catalytic activity. A subset, the RNA-cleaving NAzyme, is characterized by its substrate of choice: an RNA unit. These enzymes have been used for diverse applications, including biosensor development, akin to their protein counterparts. Owing to their function as both biorecognition elements and signal generators, robust bioassays based entirely on NAzyme molecules have been developed. Additionally, unique mechanisms for integration with other biorecognition elements and signal generation methods have been explored to realize ultrasensitive, specific, and user-friendly biosensors. Furthermore, NAzyme-based bioassays have already broken into the in vitro diagnostics market, with more promise in the pipeline.
MeSH term(s)	Biosensing Techniques/trends ; DNA, Catalytic/metabolism ; RNA/metabolism ; RNA, Catalytic/metabolism
Chemical Substances	DNA, Catalytic ; RNA, Catalytic ; RNA (63231-63-0)
Language	English
Publishing date	2020-05-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	47474-5
ISSN	1879-3096 ; 0167-7799
ISSN (online)	1879-3096
ISSN	0167-7799
DOI	10.1016/j.tibtech.2020.04.012
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Article ; Online: Multiplex Analysis to Unravel the Mode of Antifungal Activity of the Plant Defensin HsAFP1 in Single Yeast Cells.

Struyfs, Caroline / Breukers, Jolien / Spasic, Dragana / Lammertyn, Jeroen / Cammue, Bruno P A / Thevissen, Karin

International journal of molecular sciences

2022 Volume 23, Issue 3

Abstract: Single cell analyses have gained increasing interest over bulk approaches because of considerable cell-to-cell variability within isogenic populations. Herein, flow cytometry remains golden standard due to its high-throughput efficiency and versatility, ... ...

Abstract	Single cell analyses have gained increasing interest over bulk approaches because of considerable cell-to-cell variability within isogenic populations. Herein, flow cytometry remains golden standard due to its high-throughput efficiency and versatility, although it does not allow to investigate the interdependency of cellular events over time. Starting from our microfluidic platform that enables to trap and retain individual cells on a fixed location over time, here, we focused on unraveling kinetic responses of single
MeSH term(s)	Cell Membrane Permeability/drug effects ; Coral Bleaching ; Defensins/pharmacology ; Fungicides, Industrial/pharmacology ; Heuchera/metabolism ; Microbial Viability/drug effects ; Microfluidic Analytical Techniques ; Plant Proteins/pharmacology ; Reactive Oxygen Species/metabolism ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae/metabolism ; Single-Cell Analysis ; Time Factors
Chemical Substances	Defensins ; Fungicides, Industrial ; Plant Proteins ; Reactive Oxygen Species
Language	English
Publishing date	2022-01-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23031515
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Article ; Online: Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor.

Yildizhan, Yagmur / Driessens, Kaat / Tsao, Hong Shen Kevin / Boiy, Robin / Thomas, Debby / Geukens, Nick / Hendrix, An / Lammertyn, Jeroen / Spasic, Dragana

International journal of molecular sciences

2023 Volume 24, Issue 4

Abstract: Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed for EV detection, many of them are still not applicable to clinical settings as they rely on ... ...

Abstract	Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed for EV detection, many of them are still not applicable to clinical settings as they rely on complex EV isolation processes, while lacking sensitivity, specificity or standardization. To solve this problem, we have developed a sensitive breast cancer-specific EV detection bioassay directly in blood plasma using a fiber-optic surface plasmon resonance (FO-SPR) biosensor, previously calibrated with recombinant EVs. First, we established a sandwich bioassay to detect SK-BR-3 EVs by functionalizing the FO-SPR probes with anti-HER2 antibodies. A calibration curve was built using an anti-HER2/
MeSH term(s)	Female ; Humans ; Biomarkers ; Biosensing Techniques/methods ; Breast Neoplasms/diagnosis ; Extracellular Vesicles ; Surface Plasmon Resonance/methods
Chemical Substances	Biomarkers
Language	English
Publishing date	2023-02-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24043764
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