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  1. Article: Multiple genetic changes can occur in the oral poliovaccines upon replication in humans

    Obijeski, J. F.

    J. General Virology

    1981  Volume 56, Page(s) 337–347

    Abstract: Poliovirus isolates of serotypes 2 and 3 from patients whose paralytic poliomyelitis cases were classified as oral vaccine-associated were analysed by oligonucleotide mapping of the virus genomes and by polyacrylamide gel electrophoresis of the virus ... ...

    Institution USA Atlanta, GA 30333 Public Health Service, U.S. Department of Health and Human Services
    Abstract Poliovirus isolates of serotypes 2 and 3 from patients whose paralytic poliomyelitis cases were classified as oral vaccine-associated were analysed by oligonucleotide mapping of the virus genomes and by polyacrylamide gel electrophoresis of the virus proteins. Oligonucleotide maps of all isolates were similar to the maps of the corresponding oral vaccine strain. No two isolates gave identical maps. Most maps differed from that of the vaccine strain by at least one oligonucleotide spot. Maps of changes had occurred in the vaccine virus genomes during replication in one or two individuals. In contrast, maps of some neural tissue isolates showed minimal differences from the reference vaccine maps, raising the possibility that neurovirulence may be restored by a small number of genetic changes. For many isolates, changes were also detected in the mobilities and processing rates of the virus proteins.
    Keywords Poliomyelitis ; Impfung ; Impfstoff ; Oral ; Impfschaden ; Genetik
    Language English
    Document type Article
    Database Social Medicine (SOMED)

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  2. Article: Rabies virus-induced RNA synthesis in BHK21 cells.

    Holloway, B P / Obijeski, J F

    The Journal of general virology

    1980  Volume 49, Issue 1, Page(s) 181–195

    Abstract: Rabies virus polysomes contained two sizes of messenger RNAs, one of which had a sedimentation value of 30S and another which sedimented at 12 to 16S. RNA extracted from infected cultures contained virion-size RNA, 42S, as well as 30S and 12 to 16S RNA ... ...

    Abstract Rabies virus polysomes contained two sizes of messenger RNAs, one of which had a sedimentation value of 30S and another which sedimented at 12 to 16S. RNA extracted from infected cultures contained virion-size RNA, 42S, as well as 30S and 12 to 16S RNA species. Hybridization studies indicated that the 30S and 12 to 16S RNAs had nucleotide sequences which were complementary to virion RNA. RNA. RNA isolated from virus polysomes contained adenylate-rich sequences which were heterogeneous in size and were determined to be about 100 to 250 nucleotides in length on the basis of their migration rates in polyacrylamide gels. Acid-urea agarose gel electrophoresis established that the 30S RNA material was composed of a single RNA species (mol. wt. greater than or equal to 1.65 X 10(6)), whereas the 12 to 16S material could be resolved into at least four distinct species whose mol. wt. ranged from 0.28 to 0.87 X 10(6). When labelled rabies-infected cell RNAs, which were purified by oligo(dT)-cellulose chromatography, were annealed to excess unlabelled virus RNA, digested with ribonuclease T2 and the RNA duplex molecules analysed by polyacrylamide gel electrophoresis, five duplexes could be separated. The mol. wt. of these duplexes were estimated to be 3.2, 1.4, 0.96, 0.55 and 0.39 X 10(6), when compared to the known mol. wt. of vesicular stomatitis virus (VSV) RNA duplexes. This study suggests that the replicative processes of rabies virus are very similar to VSV and that rabies virus proteins are probably translated from smaller than virion-size RNAs.
    MeSH term(s) Animals ; Base Sequence ; Cell Line ; Cricetinae ; Nucleic Acid Hybridization ; Poly A ; Polyribosomes/analysis ; RNA, Messenger/biosynthesis ; RNA, Viral/analysis ; RNA, Viral/biosynthesis ; Rabies virus/metabolism
    Chemical Substances RNA, Messenger ; RNA, Viral ; Poly A (24937-83-5)
    Language English
    Publishing date 1980-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/0022-1317-49-1-181
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  3. Article: Bunyaviridae: recent biochemical developments.

    Obijeski, J F / Murphy, F A

    The Journal of general virology

    1977  Volume 37, Issue 1, Page(s) 1–14

    MeSH term(s) Arboviruses/ultrastructure ; Base Sequence ; Bunyamwera virus/genetics ; Bunyamwera virus/metabolism ; Bunyamwera virus/ultrastructure ; DNA-Directed RNA Polymerases/metabolism ; Glycoproteins/analysis ; Molecular Weight ; Morphogenesis ; Nucleic Acid Conformation ; RNA, Viral/analysis ; RNA, Viral/biosynthesis ; Viral Proteins/analysis ; Viral Proteins/biosynthesis
    Chemical Substances Glycoproteins ; RNA, Viral ; Viral Proteins ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 1977-10
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/0022-1317-37-1-1
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  4. Article: High resolution polyacrylamide gradient gel electrophoresis.

    Esposito, J J / Obijeski, J F

    Preparative biochemistry

    1976  Volume 6, Issue 6, Page(s) 431–442

    Abstract: A method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. Well-defined protein zones were observed and improved ... ...

    Abstract A method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. Well-defined protein zones were observed and improved resolution was attained especially for low molecular weight proteins in preparations containing a variety of polypeptides, e.g. viruses that are often separated by continuous gel methods. Electropherograms of continuous (8%) and gradient (3 to 30%) gels were made of purified vesicular stomatitis virus, variola virus, Rickettsia rickettsii, and alpha and beta chains of hemoglobin in order to demonstrate the resolution of the gradient system.
    MeSH term(s) Acrylamides ; Electrophoresis, Polyacrylamide Gel/methods ; Molecular Weight ; Peptides/isolation & purification ; Proteins/isolation & purification ; Species Specificity ; Viral Proteins/isolation & purification
    Chemical Substances Acrylamides ; Peptides ; Proteins ; Viral Proteins
    Language English
    Publishing date 1976
    Publishing country United States
    Document type Journal Article
    ZDB-ID 120528-6
    ISSN 0032-7484
    ISSN 0032-7484
    DOI 10.1080/00327487608069128
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  5. Article: Can variola-like viruses be derived from monkeypox virus? An investigation based on DNA mapping.

    Esposito, J J / Nakano, J H / Obijeski, J F

    Bulletin of the World Health Organization

    1985  Volume 63, Issue 4, Page(s) 695–703

    Abstract: The results are presented of a special study to determine whether variola-like "whitepox" viruses could arise as white pock variants of monkeypox virus after one or a few mutations. DNA mapping by cross-hybridization of restriction endonuclease DNA ... ...

    Abstract The results are presented of a special study to determine whether variola-like "whitepox" viruses could arise as white pock variants of monkeypox virus after one or a few mutations. DNA mapping by cross-hybridization of restriction endonuclease DNA fragments was carried out on 18 orthopoxviruses relevant to this study, including variola and monkeypox viruses and white (non-haemorrhagic) pock producers recovered from chorioallantoic membranes infected with red (haemorrhagic) pock-producing monkeypox viruses. The distinctiveness of the DNA maps of true variola and monkeypox viruses indicated that spontaneous production of "whitepox" from monkeypox virus was genetically impossible. These and other observations led to the conclusion that the "whitepox" viruses recovered from monkeypox virus stocks had an exogenous origin.
    MeSH term(s) Animals ; Chromosome Mapping ; Genetic Variation ; Monkeypox virus/genetics ; Mutation ; Poxviridae/genetics ; Variola virus/genetics
    Language English
    Publishing date 1985
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 80213-x
    ISSN 1564-0604 ; 0042-9686 ; 0366-4996 ; 0510-8659
    ISSN (online) 1564-0604
    ISSN 0042-9686 ; 0366-4996 ; 0510-8659
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  6. Article: Conditional lethal mutants of vesicular stomatitis virus. II. Synthesis of virus-specific polypeptides in nonpermissive cells infected with "RNA-" host-restricted mutants.

    Obijeski, J F / Simpson, R W

    Virology

    1974  Volume 57, Issue 2, Page(s) 369–377

    MeSH term(s) Animals ; Carbon Radioisotopes ; Carcinoma ; Cell Line ; Chick Embryo ; Culture Techniques ; Dactinomycin/pharmacology ; Electrophoresis, Polyacrylamide Gel ; Fibroblasts ; Fluorescent Antibody Technique ; Humans ; Laryngeal Neoplasms ; Mutation ; Peptide Biosynthesis ; Phenotype ; RNA, Viral/biosynthesis ; Transcription, Genetic ; Tritium ; Uridine/metabolism ; Vesicular stomatitis Indiana virus/growth & development ; Vesicular stomatitis Indiana virus/metabolism ; Viral Proteins/biosynthesis ; Virus Replication
    Chemical Substances Carbon Radioisotopes ; RNA, Viral ; Viral Proteins ; Tritium (10028-17-8) ; Dactinomycin (1CC1JFE158) ; Uridine (WHI7HQ7H85)
    Language English
    Publishing date 1974-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/0042-6822(74)90176-7
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  7. Article: Conditional lethal mutants of vesicular stomatitis virus. I. Phenotypic characterization of single and double mutants exhibiting host restriction and temperature sensitivity.

    Simpson, R W / Obijeski, J F

    Virology

    1974  Volume 57, Issue 2, Page(s) 357–368

    MeSH term(s) Animals ; Carcinoma ; Cell Line ; Cell-Free System ; Chick Embryo ; Culture Techniques ; DNA-Directed RNA Polymerases/metabolism ; Dactinomycin/pharmacology ; Fibroblasts ; Fluorouracil ; HeLa Cells ; Humans ; Laryngeal Neoplasms ; Mutagens ; Mutation ; Nitrosoguanidines ; Phenotype ; RNA, Viral/biosynthesis ; Temperature ; Tritium ; Uridine/metabolism ; Vesicular stomatitis Indiana virus/enzymology ; Vesicular stomatitis Indiana virus/growth & development ; Vesicular stomatitis Indiana virus/metabolism ; Viral Plaque Assay ; Virus Replication
    Chemical Substances Mutagens ; Nitrosoguanidines ; RNA, Viral ; Tritium (10028-17-8) ; Dactinomycin (1CC1JFE158) ; DNA-Directed RNA Polymerases (EC 2.7.7.6) ; Fluorouracil (U3P01618RT) ; Uridine (WHI7HQ7H85)
    Language English
    Publishing date 1974-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/0042-6822(74)90175-5
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  8. Article: Rotaviruses: a review.

    Estes, M K / Palmer, E L / Obijeski, J F

    Current topics in microbiology and immunology

    1983  Volume 105, Page(s) 123–184

    MeSH term(s) Adult ; Animals ; Genes, Viral ; Humans ; Infant, Newborn ; Rotavirus/genetics ; Rotavirus/pathogenicity ; Rotavirus/physiology ; Rotavirus Infections/diagnosis ; Rotavirus Infections/immunology ; Rotavirus Infections/microbiology ; Viral Proteins/isolation & purification ; Virus Cultivation ; Virus Replication
    Chemical Substances Viral Proteins
    Language English
    Publishing date 1983
    Publishing country Germany
    Document type Comparative Study ; Journal Article ; Review
    ISSN 0070-217X
    ISSN 0070-217X
    DOI 10.1007/978-3-642-69159-1_3
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  9. Article: Molecular cloning and sequencing of the La Crosse virus S RNA.

    Cabradilla, C D / Holloway, B P / Obijeski, J F

    Virology

    1983  Volume 128, Issue 2, Page(s) 463–468

    Abstract: Complete double-stranded DNA copies of the La Crosse virus (LAC) S genome have been synthesized and cloned into plasmid pBR322. The cloned genome was characterized and sequenced. The LAC S genome consisted of 981 nucleotides and contained two overlapping ...

    Abstract Complete double-stranded DNA copies of the La Crosse virus (LAC) S genome have been synthesized and cloned into plasmid pBR322. The cloned genome was characterized and sequenced. The LAC S genome consisted of 981 nucleotides and contained two overlapping open reading frames. The first reading frame begins at nucleotide 82 and encodes a protein of 235 amino acids. A polypeptide of 92 amino acids can be translated in a +1 reading frame 16 nucleotides downstream from the start of the first reading frame. This second reading frame is initiated with two AUG codons followed by the serine codon UCG, the same serine codon which immediately follows the AUG of the first reading frame.
    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Bunyaviridae/genetics ; Capsid/genetics ; Cloning, Molecular ; Codon ; Encephalitis Virus, California/genetics ; Genes, Viral ; Protein Biosynthesis ; RNA, Messenger/genetics ; RNA, Viral/genetics ; Viral Proteins/genetics
    Chemical Substances Codon ; RNA, Messenger ; RNA, Viral ; Viral Proteins
    Language English
    Publishing date 1983-07-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/0042-6822(83)90271-4
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  10. Article: Nucleotide sequences at the terminal of La Crosse virus RNAs.

    Obijeski, J F / McCauley, J / Skehel, J J

    Nucleic acids research

    1980  Volume 8, Issue 11, Page(s) 2431–2438

    Abstract: The 5' and 3'-terminal sequences of the three RNA molecules which make up the genomes of La Crosse virus are reported. Eleven nucleotides at both the 5' and 3' termini of all three RNAs are conserved and complementary. In addition more extensive unique ... ...

    Abstract The 5' and 3'-terminal sequences of the three RNA molecules which make up the genomes of La Crosse virus are reported. Eleven nucleotides at both the 5' and 3' termini of all three RNAs are conserved and complementary. In addition more extensive unique sequence complementarity is present in at least two of the three RNAs.
    MeSH term(s) Base Sequence ; Bunyaviridae ; Encephalitis Virus, California ; Isotope Labeling ; Phosphorus Radioisotopes ; RNA, Viral ; Ribonucleases
    Chemical Substances Phosphorus Radioisotopes ; RNA, Viral ; Ribonucleases (EC 3.1.-)
    Language English
    Publishing date 1980-06-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 186809-3
    ISSN 0305-1048 ; 0301-5610
    ISSN 0305-1048 ; 0301-5610
    DOI 10.1093/nar/8.11.2431
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