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Article ; Online: Early prehabilitation reduces admissions and time in hospital in patients with newly diagnosed lung cancer.

Phillips, Iain / Deans, Maria / Walton, Abi / Vallet, Mahéva / Mencnarowksi, Julie / McMillan, Debbie / Peacock, Catriona / Hall, Peter / O'Brien, Fiona / Stares, Mark / Mackean, Melanie / Plant, Tracie / Grecian, Robert / Allan, Lindsey / Petrie, Rebecca / Blues, Duncan / Haddad, Suraiya / Barrie, Colin

BMJ supportive & palliative care

2024

Abstract: ... in the prehabilitation group (0.73 vs 0.41 years, p=0.046).: Conclusions: Early prehabilitation appears to reduce time ... compared with 178 prehab eligible historical controls diagnosed from 2019 to 2021.: Results: From July ...

Abstract	Objectives: Lung cancer is the leading cause of cancer death in the UK. Prehabilitation aims to maximise patient fitness and minimise the negative impact of anticancer treatment. What constitutes prehabilitation before non-surgical anticancer treatment is not well established. We present data from a pilot project of Early prehabilitation In lung Cancer. Methods: All new patients with likely advanced lung cancer were offered prehabilitation at respiratory clinic, if fit for further investigation. Prehabilitation included assessment and appropriate intervention from a consultant in palliative medicine, registered dietitian and rehabilitation physiotherapist. Four objective endpoints were identified, namely admissions to hospital, time spent in the hospital, treatment rates and overall survival. Outcomes were to be compared with 178 prehab eligible historical controls diagnosed from 2019 to 2021. Results: From July 2021 to June 2023, 65 patients underwent prehabilitation and 72% of patients underwent all 3 interventions. 54 patients had a stage 3 or 4 lung cancer. In the prehab group, fewer patients attended Accident and Emergency (31.5 vs 37.4 attendances per 100 patients) and fewer were admitted (51.9 vs 67.9) when compared with historical controls. Those receiving prehab spent a lot less time in the hospital (129.7 vs 543.5 days per 100 patients) with shorter admissions (2.5 vs 8 days). Systemic anticancer treatment rates increased in the short term but were broadly similar overall. Median survival was higher in the prehabilitation group (0.73 vs 0.41 years, p=0.046). Conclusions: Early prehabilitation appears to reduce time spent in the hospital. It may improve survival. Further work is required to understand its full effect on treatment rates.
Language	English
Publishing date	2024-04-17
Publishing country	England
Document type	Journal Article
ISSN	2045-4368
ISSN (online)	2045-4368
DOI	10.1136/spcare-2024-004869
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Article: FMC7 is an epitope of CD20.

Deans, Julie P / Polyak, Maria J

Blood

2008 Volume 111, Issue 4, Page(s) 2492; author reply 2493–4

MeSH term(s)	Antibodies, Monoclonal/therapeutic use ; Antibodies, Monoclonal, Murine-Derived ; Antigens, CD20/analysis ; Antineoplastic Agents/therapeutic use ; Epitopes/analysis ; Glycoproteins/analysis ; Humans ; Immunophenotyping ; K562 Cells ; Lymphoma, B-Cell/drug therapy ; Rituximab
Chemical Substances	Antibodies, Monoclonal ; Antibodies, Monoclonal, Murine-Derived ; Antigens, CD20 ; Antineoplastic Agents ; Epitopes ; Glycoproteins ; MS4A1 protein, human ; Rituximab (4F4X42SYQ6)
Language	English
Publishing date	2008-01-13
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood-2007-11-126243
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Article: First evidence of protein-carbohydrate regulation in a plant bug (Lygus hesperus)

Deans, Carrie / Behmer, Spencer T / Sword, Gregory A

Journal of insect physiology. 2019 July, v. 116

2019

Abstract: ... macronutrients – protein (p) and carbohydrates (c) – that are tightly linked to survival and performance ...

Abstract	Lygus bugs are highly polyphagous piercing/sucking insects found throughout North America. Collectively, they have been reported to feed on over 330 plant species (one of the broadest host range ever documented for a group of insects); they also feed on many economically important crops. Despite its prevalence across North America and status as a common pest in many agroecosystems, very little is known about how Lygus bugs regulate their intake of nutrients. In reality, little is known about nutrient regulation for most hemipterans, specifically non-phloem feeding species in the suborder Heteroptera. This likely reflects difficulties in developing adequate artificial diets for insects with piercing/sucking mouthparts. There is, however, an artificial diet for L. hersperus, and in this study we modified it and performed choice and no-choice experiments to determine how L. hesperus regulates its intake of two macronutrients – protein (p) and carbohydrates (c) – that are tightly linked to survival and performance in other insect herbivores. In choice experiments L. hesperus was allowed to select between two foods with different protein:carbohydrate ratios. We documented strong regulation for protein and carbohydrates, with late instar nymphs selecting a slightly protein-biased intake target (protein-carbohydrate ratio = 1.5:1). We also performed no-choice experiments, where nymphs were restricted to a single food. Here, the protein-carbohydrate ratio of their food had a strong impact on survival, which was highest for nymphs reared on the treatment with a protein-carbohydrate ratio closest to the self-selected intake target (determined by the choice experiments), but no significant impact on developmental time or mass gain. Our data are the first of their kind for a non-phloem feeding hemipteran and provide a starting point for more broadly understanding and further investigating the nutritional ecology/physiology of Lygus bugs. Our study also provides a framework for exploring nutrient regulation in other hemipterans and for optimizing artificial diets for piercing/sucking insects, especially heteropterans.
Keywords	agroecosystems ; artificial diets ; carbohydrates ; crops ; foods ; host range ; insect physiology ; instars ; Lygus hesperus ; mouthparts ; nutrients ; nymphs ; pests ; phytophagous insects ; rearing ; North America
Language	English
Dates of publication	2019-07
Size	p. 118-124.
Publishing place	Elsevier Ltd
Document type	Article
ISSN	0022-1910
DOI	10.1016/j.jinsphys.2019.05.004
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Article ; Online: Asherman syndrome: Audit of a single-operator cohort of 423 cases.

Vancaillie, Thierry / Chan, Karen / Liu, Jinzhu / Deans, Rebecca / Howard, Elizabeth

The Australian & New Zealand journal of obstetrics & gynaecology

2020 Volume 60, Issue 4, Page(s) 574–578

Abstract: ... with a pregnancy rate of 94.5% (P = 0.029).: Conclusion: Asherman syndrome should be considered in any woman ... of a large cohort of patients treated by a single operator.: Materials and methods: From July 1998 ...

Abstract	Background: The diagnosis of Asherman syndrome, or 'intra-uterine adhesions' is often overlooked when the symptoms of amenorrhea and hematometra are missing. Aims: This audit reviews the clinical data of a large cohort of patients treated by a single operator. Materials and methods: From July 1998 till the end of December 2017, 423 patients with intra-uterine adhesions were treated by a single operator. Clinical information was obtained by review of the medical files and phone interviews. Results: Amenorrhea was recorded in 163/423 patients (38.5%), 225/423 (53.2%) patients did not have amenorrhea and for 35/423 (8.3%) patients the information was missing. A hematometra was documented in 19/423 (4.5%) patients. Pregnancy was achieved in 215/246 (87.4%). Patients with stage II disease did best with a pregnancy rate of 94.5% (P = 0.029). Conclusion: Asherman syndrome should be considered in any woman with a history of miscarriage or postpartum curettage who then fails to conceive again.
MeSH term(s)	Amenorrhea/etiology ; Dilatation and Curettage ; Female ; Gynatresia/epidemiology ; Gynatresia/etiology ; Gynatresia/surgery ; Humans ; Pregnancy ; Tissue Adhesions/complications ; Uterine Diseases/surgery
Language	English
Publishing date	2020-05-26
Publishing country	Australia
Document type	Journal Article
ZDB-ID	390815-x
ISSN	1479-828X ; 0004-8666
ISSN (online)	1479-828X
ISSN	0004-8666
DOI	10.1111/ajo.13182
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Article ; Online: Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells.

Zuccolo, Jonathan / Unruh, Tammy L / Deans, Julie P

BMC immunology

2009 Volume 10, Page(s) 30

Abstract: Background: Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate ... ...

Abstract	Background: Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocytes from tonsils in a short and cost-effective single step, using a commercially available reagent designed for purifying cells from whole blood (RosetteSep). This technique relies on the presence of the large excess of red blood cells in whole blood for the formation of immunorosettes, whereas single cell suspensions from tonsils contain relatively few red blood cells. Results: B cell enrichment from tonsils was achieved using RosetteSep with no modification to the whole blood procedure; however, the degree of purity depended on the extent of red blood cell contamination of the starting tonsil cell suspension. Addition of a 50-fold excess of allogeneic human red blood cells, but not sheep red blood cells, reproducibly resulted in high levels of purity. Depletion of mononuclear cells from the donor red blood cells eliminated potential contamination with allogeneic B cells. Conclusion: RosetteSep reagent can be used in combination with allogeneic human red blood cells to reproducibly isolate tonsil B lymphocytes to high levels of purity with no change in phenotype or loss of cells. This method provides considerable time and cost savings compared to other methods.
MeSH term(s)	Animals ; B-Lymphocytes/cytology ; B-Lymphocytes/immunology ; Cell Separation ; Cost-Benefit Analysis ; Erythrocytes/immunology ; Erythrocytes/metabolism ; HLA Antigens ; Humans ; Immunologic Techniques/economics ; Palatine Tonsil/cytology ; Rosette Formation ; Sheep ; Time Factors
Chemical Substances	HLA Antigens
Language	English
Publishing date	2009-05-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1471-2172
ISSN (online)	1471-2172
DOI	10.1186/1471-2172-10-30
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Article: Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts.

Petrie, Ryan J / Deans, Julie P

Journal of immunology (Baltimore, Md. : 1950)

2002 Volume 169, Issue 6, Page(s) 2886–2891

Abstract: The B cell Ag receptor (BCR) and CD20, a putative calcium channel, inducibly associate with cholesterol-dependent membrane microdomains known as lipid rafts. A functional association between the BCR and CD20 is suggested by the effects of CD20-specific ... ...

Abstract	The B cell Ag receptor (BCR) and CD20, a putative calcium channel, inducibly associate with cholesterol-dependent membrane microdomains known as lipid rafts. A functional association between the BCR and CD20 is suggested by the effects of CD20-specific mAbs, which can modulate cell cycle transitions elicited by BCR signaling. Using immunofluorescence microscopy we show here that the BCR and CD20 colocalize after receptor ligation and then rapidly dissociate at the cell surface before endocytosis of the BCR. After separation, surface BCR and CD20 were detected in distinct lipid rafts isolated as low density, detergent-resistant membrane fragments. Pretreatment with methyl-beta-cyclodextrin, which we have previously shown to enhance receptor-mediated calcium mobilization, did not prevent colocalization of the BCR and CD20, but slowed their dissociation. The data demonstrate rapid dynamics of the BCR in relation to CD20 at the cell surface. Activation-dependent dissociation of the BCR from CD20 occurs before receptor endocytosis and appears to require in part the integrity of lipid rafts.
MeSH term(s)	Antigens, CD20/immunology ; Antigens, CD20/metabolism ; B-Lymphocytes/drug effects ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; Cell Membrane/immunology ; Cell Membrane/metabolism ; Cyclodextrins/pharmacology ; Detergents/pharmacology ; Endocytosis/drug effects ; Endocytosis/immunology ; Humans ; Kinetics ; Lymphocyte Activation ; Membrane Microdomains/drug effects ; Membrane Microdomains/immunology ; Membrane Microdomains/metabolism ; Microscopy, Fluorescence ; Receptors, Antigen, B-Cell/antagonists & inhibitors ; Receptors, Antigen, B-Cell/immunology ; Receptors, Antigen, B-Cell/metabolism ; Tumor Cells, Cultured ; beta-Cyclodextrins
Chemical Substances	Antigens, CD20 ; Cyclodextrins ; Detergents ; Receptors, Antigen, B-Cell ; beta-Cyclodextrins ; methyl-beta-cyclodextrin
Language	English
Publishing date	2002-08-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.169.6.2886
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Article ; Online: Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells

Deans Julie P / Unruh Tammy L / Zuccolo Jonathan

BMC Immunology, Vol 10, Iss 1, p

2009 Volume 30

Abstract: Abstract Background Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to ... ...

Abstract	Abstract Background Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocytes from tonsils in a short and cost-effective single step, using a commercially available reagent designed for purifying cells from whole blood (RosetteSep). This technique relies on the presence of the large excess of red blood cells in whole blood for the formation of immunorosettes, whereas single cell suspensions from tonsils contain relatively few red blood cells. Results B cell enrichment from tonsils was achieved using RosetteSep with no modification to the whole blood procedure; however, the degree of purity depended on the extent of red blood cell contamination of the starting tonsil cell suspension. Addition of a 50-fold excess of allogeneic human red blood cells, but not sheep red blood cells, reproducibly resulted in high levels of purity. Depletion of mononuclear cells from the donor red blood cells eliminated potential contamination with allogeneic B cells. Conclusion RosetteSep reagent can be used in combination with allogeneic human red blood cells to reproducibly isolate tonsil B lymphocytes to high levels of purity with no change in phenotype or loss of cells. This method provides considerable time and cost savings compared to other methods.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Allergy and Immunology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	610
Language	English
Publishing date	2009-05-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.

Polyak, Maria J / Deans, Julie P

Blood

2002 Volume 99, Issue 9, Page(s) 3256–3262

Abstract: In vivo ablation of malignant B cells can be achieved using antibodies directed against the CD20 antigen. Fine specificity differences among CD20 monoclonal antibodies (mAbs) are assumed not to be a factor in determining their efficacy because evidence ... ...

Abstract	In vivo ablation of malignant B cells can be achieved using antibodies directed against the CD20 antigen. Fine specificity differences among CD20 monoclonal antibodies (mAbs) are assumed not to be a factor in determining their efficacy because evidence from antibody-blocking studies indicates limited epitope diversity with only 2 overlapping extracellular CD20 epitopes. However, in this report a high degree of heterogeneity among antihuman CD20 mAbs is demonstrated. Mutation of alanine and proline at positions 170 and 172 (AxP) (single-letter amino acid codes; x indicates the identical amino acid at the same position in the murine and human CD20 sequences) in human CD20 abrogated the binding of all CD20 mAbs tested. Introduction of AxP into the equivalent positions in the murine sequence, which is not otherwise recognized by antihuman CD20 mAbs, fully reconstituted the epitope recognized by B1, the prototypic anti-CD20 mAb. 2H7, a mAb previously thought to recognize the same epitope as B1, did not recognize the murine AxP mutant. Reconstitution of the 2H7 epitope was achieved with additional mutations replacing VDxxD in the murine sequence for INxxN (positions 162-166 in the human sequence). The integrity of the 2H7 epitope, unlike that of B1, further depends on the maintenance of CD20 in an oligomeric complex. The majority of 16 antihuman CD20 mAbs tested, including rituximab, bound to murine CD20 containing the AxP mutations. Heterogeneity in the fine specificity of these antibodies was indicated by marked differences in their ability to induce homotypic cellular aggregation and translocation of CD20 to a detergent-insoluble membrane compartment previously identified as lipid rafts.
MeSH term(s)	Alanine ; Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/chemistry ; Antibodies, Monoclonal/immunology ; Antibody Specificity ; Antigens, CD20/immunology ; Antigens, CD20/metabolism ; Cell Membrane/metabolism ; Epitope Mapping ; Epitopes/chemistry ; Epitopes/immunology ; Epitopes/metabolism ; Humans ; Membrane Microdomains/metabolism ; Mice ; Molecular Sequence Data ; Mutagenesis ; Proline ; Protein Structure, Quaternary ; Tumor Cells, Cultured
Chemical Substances	Antibodies, Monoclonal ; Antigens, CD20 ; Epitopes ; Proline (9DLQ4CIU6V) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2002-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood.v99.9.3256
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Article: Determination of apparent calcium affinity for endogenously expressed human sarco(endo)plasmic reticulum calcium-ATPase isoform SERCA3.

Chandrasekera, P Charukeshi / Kargacin, Margaret E / Deans, Julie P / Lytton, Jonathan

American journal of physiology. Cell physiology

2009 Volume 296, Issue 5, Page(s) C1105–14

Abstract: The sarco(endo)plasmic reticulum Ca(2+)-ATPases (SERCAs) play a crucial role in regulating free cytosolic Ca(2+) concentration in diverse cell types. It has been shown that recombinant SERCA3, when measured in heterologous systems, exhibits low apparent ... ...

Abstract	The sarco(endo)plasmic reticulum Ca(2+)-ATPases (SERCAs) play a crucial role in regulating free cytosolic Ca(2+) concentration in diverse cell types. It has been shown that recombinant SERCA3, when measured in heterologous systems, exhibits low apparent affinity for Ca(2+); however, Ca(2+) affinity of native SERCA3 in an endogenous setting has not been examined. Such a measurement is complicated, because SERCA3 is always coexpressed with the housekeeping isoform SERCA2b. We used a fluorescence-based assay for monitoring continuous Ca(2+) uptake into microsomes to examine the properties of endogenous human SERCA3 and SERCA2b. The kinetic parameters were derived using a cooperative two-component uptake model for Ca(2+) activation, and the values assigned to SERCA3 were confirmed using the highly specific human SERCA3 inhibitory antibody PL/IM430. First, using recombinant human SERCA3 and SERCA2b proteins transiently expressed in HEK-293 cells, we confirmed the previously observed low apparent Ca(2+) affinity for SERCA3 compared with SERCA2b (1.10 +/- 0.04 vs. 0.26 +/- 0.01 microM), and using mixtures of recombinant protein isoforms, we validated the two-component uptake model. Then we determined apparent Ca(2+) affinity for SERCA proteins present endogenously in cultured Jurkat T lymphocytes and freshly isolated human tonsil lymphocytes. The apparent Ca(2+) affinity in these two preparations was 1.04 +/- 0.07 and 1.1 +/- 0.2 muM for SERCA3 and 0.27 +/- 0.02 and 0.26 +/- 0.01 microM for SERCA2b, respectively. Our data demonstrate, for the first time, that affinity for Ca(2+) is inherently lower for SERCA3 expressed in situ than for other SERCA isoforms.
MeSH term(s)	Antibodies/pharmacology ; Calcium/pharmacokinetics ; Calcium Signaling/physiology ; Cytosol/metabolism ; Humans ; Jurkat Cells ; Kidney/cytology ; Microsomes/metabolism ; Models, Biological ; Palatine Tonsil/cytology ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/immunology ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism ; T-Lymphocytes/cytology ; T-Lymphocytes/metabolism
Chemical Substances	Antibodies ; Recombinant Proteins ; Sarcoplasmic Reticulum Calcium-Transporting ATPases (EC 3.6.3.8) ; ATP2A2 protein, human (EC 7.2.2.10) ; ATP2A3 protein, human (EC 7.2.2.10) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2009-02-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00650.2008
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Article: CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins.

Polyak, Maria J / Li, Haidong / Shariat, Neda / Deans, Julie P

The Journal of biological chemistry

2008 Volume 283, Issue 27, Page(s) 18545–18552

Abstract: B cell antigen receptor (BCR) signaling initiates sustained cellular calcium influx necessary for the development, differentiation, and activation of B lymphocytes. CD20 is a B cell-restricted tetraspanning protein organized in the plasma membrane as ... ...

Abstract	B cell antigen receptor (BCR) signaling initiates sustained cellular calcium influx necessary for the development, differentiation, and activation of B lymphocytes. CD20 is a B cell-restricted tetraspanning protein organized in the plasma membrane as multimeric molecular complexes involved in BCR-activated calcium entry. Using coprecipitation of native CD20 with tagged or truncated forms of the molecule, we provide here direct evidence of CD20 homo-oligomerization into tetramers. Additionally, the function of CD20 was explored by examining its association with surface-labeled and intracellular proteins before and after BCR signaling. Two major surface-labeled proteins that coprecipitated with CD20 were identified as the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR. After activation, BCR-CD20 complexes dissociated, and phosphoproteins and calmodulin-binding proteins were transiently recruited to CD20. These data provide new evidence of the involvement of CD20 in signaling downstream of the BCR and, together with the previously described involvement of CD20 in calcium influx, the first evidence of physical coupling of the BCR to a calcium entry pathway.
MeSH term(s)	Animals ; Antigens, CD20/immunology ; Antigens, CD20/metabolism ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; Calcium/immunology ; Calcium/metabolism ; Calcium Signaling/physiology ; Calmodulin-Binding Proteins/immunology ; Calmodulin-Binding Proteins/metabolism ; Cell Line, Tumor ; Cell Membrane/immunology ; Cell Membrane/metabolism ; Immunoglobulin M/immunology ; Immunoglobulin M/metabolism ; Lymphocyte Activation/physiology ; Mice ; Protein Structure, Quaternary/physiology ; Receptors, Antigen, B-Cell/immunology ; Receptors, Antigen, B-Cell/metabolism
Chemical Substances	Antigens, CD20 ; Calmodulin-Binding Proteins ; Immunoglobulin M ; Receptors, Antigen, B-Cell ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2008-05-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M800784200
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