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  1. Article ; Online: Dataset for rapid state of health estimation of lithium batteries using EIS and machine learning: Training and validation.

    Rashid, Muhammad / Faraji-Niri, Mona / Sansom, Jonathan / Sheikh, Muhammad / Widanage, Dhammika / Marco, James

    Data in brief

    2023  Volume 48, Page(s) 109157

    Abstract: This article addresses the objective, experimental design and methodology of the tests conducted for battery State of Health (SOH) estimation using an accelerated test method. For this purpose, 25 unused cylindrical cells were aged, by continual ... ...

    Abstract This article addresses the objective, experimental design and methodology of the tests conducted for battery State of Health (SOH) estimation using an accelerated test method. For this purpose, 25 unused cylindrical cells were aged, by continual electrical cycling using a 0.5C charge and 1C discharge to 5 different SOH breakpoints (80, 85, 90, 95 and 100%). Ageing of the cells to the different SOH values was undertaken at a temperature of 25 °C. A reference performance test (RPT) of C/3 charge-discharge at 25 °C was performed when the cells were new and at each stage of cycling to define the energy capacity reduction due to increased charge-throughput. An electrochemical impedance spectroscopy (EIS) test was performed at 5, 20, 50, 70 and 95% states of charge (SOC) for each cell at temperatures of 15, 25 and 35 °C. The shared data includes the raw data files for the reference test and the measured energy capacity and the measured SOH for each cell. It contains the 360 EIS data files and a file which tabulates the key features of the EIS plot for each test case. The reported data has been used to train a machine-learning model for the rapid estimation of battery SOH discussed in the manuscript co-submitted (MF Niri et al., 2022). The reported data can be used for the creation and validation of battery performance and ageing models to underpin different application studies and the design of control algorithms to be employed in battery management systems (BMS).
    Language English
    Publishing date 2023-04-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2023.109157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Using L. minor and C. elegans to assess the ecotoxicity of real-life contaminated soil samples and their remediation by clay- and carbon-based sorbents.

    Rivenbark, Kelly J / Fawkes, Leanne S / Nikkhah, Hasan / Wang, Meichen / Sansom, Garett T / Beykal, Burcu / Wade, Terry L / Phillips, Timothy D

    Environmental pollution (Barking, Essex : 1987)

    2024  Volume 347, Page(s) 123762

    Abstract: Toxic substances, such as polycyclic aromatic hydrocarbons (PAHs) and heavy metals, can accumulate in soil, posing a risk to human health and the environment. To reduce the risk of exposure, rapid identification and remediation of potentially hazardous ... ...

    Abstract Toxic substances, such as polycyclic aromatic hydrocarbons (PAHs) and heavy metals, can accumulate in soil, posing a risk to human health and the environment. To reduce the risk of exposure, rapid identification and remediation of potentially hazardous soils is necessary. Adsorption of contaminants by activated carbons and clay materials is commonly utilized to decrease the bioavailability of chemicals in soil and environmental toxicity in vitro, and this study aims to determine their efficacy in real-life soil samples. Two ecotoxicological models (Lemna minor and Caenorhabditis elegans) were used to test residential soil samples, known to contain an average of 5.3, 262, and 9.6 ppm of PAHs, lead, and mercury, for potential toxicity. Toxicity testing of these soils indicated that 86% and 58% of soils caused ≤50% inhibition of growth and survival of L. minor and C. elegans, respectively. Importantly, 3 soil samples caused ≥90% inhibition of growth in both models, and the toxicity was positively correlated with levels of heavy metals. These toxic soil samples were prioritized for remediation using activated carbon and SM-Tyrosine sorbents, which have been shown to immobilize PAHs and heavy metals, respectively. The inclusion of low levels of SM-Tyrosine protected the growth and survival of L. minor and C. elegans by 83% and 78%, respectively from the polluted soil samples while activated carbon offered no significant protection. These results also indicated that heavy metals were the driver of toxicity in the samples. Results from this study demonstrate that adsorption technologies are effective strategies for remediating complex, real-life soil samples contaminated with hazardous pollutants and protecting natural soil and groundwater resources and habitats. The results highlight the applicability of these ecotoxicological models as rapid screening tools for monitoring soil quality and verifying the efficacy of remediation practices.
    MeSH term(s) Animals ; Humans ; Clay ; Caenorhabditis elegans ; Charcoal ; Metals, Heavy/toxicity ; Metals, Heavy/analysis ; Polycyclic Aromatic Hydrocarbons/analysis ; Soil/chemistry ; Tyrosine ; Soil Pollutants/analysis ; Araceae
    Chemical Substances Clay (T1FAD4SS2M) ; Charcoal (16291-96-6) ; Metals, Heavy ; Polycyclic Aromatic Hydrocarbons ; Soil ; Tyrosine (42HK56048U) ; Soil Pollutants
    Language English
    Publishing date 2024-03-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 280652-6
    ISSN 1873-6424 ; 0013-9327 ; 0269-7491
    ISSN (online) 1873-6424
    ISSN 0013-9327 ; 0269-7491
    DOI 10.1016/j.envpol.2024.123762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: KRAS allelic imbalance drives tumour initiation yet suppresses metastasis in colorectal cancer in vivo.

    Najumudeen, Arafath K / Fey, Sigrid K / Millett, Laura M / Ford, Catriona A / Gilroy, Kathryn / Gunduz, Nuray / Ridgway, Rachel A / Anderson, Eve / Strathdee, Douglas / Clark, William / Nixon, Colin / Morton, Jennifer P / Campbell, Andrew D / Sansom, Owen J

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 100

    Abstract: Oncogenic KRAS mutations are well-described functionally and are known to drive tumorigenesis. Recent reports describe a significant prevalence of KRAS allelic imbalances or gene dosage changes in human cancers, including loss of the wild-type allele in ... ...

    Abstract Oncogenic KRAS mutations are well-described functionally and are known to drive tumorigenesis. Recent reports describe a significant prevalence of KRAS allelic imbalances or gene dosage changes in human cancers, including loss of the wild-type allele in KRAS mutant cancers. However, the role of wild-type KRAS in tumorigenesis and therapeutic response remains elusive. We report an in vivo murine model of colorectal cancer featuring deletion of wild-type Kras in the context of oncogenic Kras. Deletion of wild-type Kras exacerbates oncogenic KRAS signalling through MAPK and thus drives tumour initiation. Absence of wild-type Kras potentiates the oncogenic effect of KRASG12D, while incidentally inducing sensitivity to inhibition of MEK1/2. Importantly, loss of the wild-type allele in aggressive models of KRASG12D-driven CRC significantly alters tumour progression, and suppresses metastasis through modulation of the immune microenvironment. This study highlights the critical role for wild-type Kras upon tumour initiation, progression and therapeutic response in Kras mutant CRC.
    MeSH term(s) Humans ; Mice ; Animals ; Proto-Oncogene Proteins p21(ras)/genetics ; Allelic Imbalance ; Genes, ras ; Cell Transformation, Neoplastic/genetics ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Mutation ; Tumor Microenvironment/genetics
    Chemical Substances Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2) ; KRAS protein, human
    Language English
    Publishing date 2024-01-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-44342-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A Review of Scheduling Strategies for Radiotherapy and Immune Checkpoint Inhibition in Locally Advanced Rectal Cancer.

    Melissourgou-Syka, Lydia / Gillespie, Michael A / O'Cathail, Sean M / Sansom, Owen J / Steele, Colin W / Roxburgh, Campbell S D

    Journal of immunotherapy and precision oncology

    2023  Volume 6, Issue 4, Page(s) 187–197

    Abstract: Colorectal cancer (CRC) is the third most common malignancy across the globe and, despite advances in treatment strategies, survival rates remain low. Rectal cancer (RC) accounts for most of these cases, and traditional management strategies for advanced ...

    Abstract Colorectal cancer (CRC) is the third most common malignancy across the globe and, despite advances in treatment strategies, survival rates remain low. Rectal cancer (RC) accounts for most of these cases, and traditional management strategies for advanced disease include total neoadjuvant therapy (TNT) with chemoradiotherapy followed by curative surgery. Unfortunately, approximately 10-15% of patients have no response to treatment or have recurrence at a short interval following radiotherapy. The introduction of immunotherapy in the form of immune checkpoint blockade (ICB) in metastatic colorectal cancer has improved clinical outcomes, yet most patients with RC present with microsatellite stable disease, which lacks the immune-rich microenvironment where ICB is most effective. There is evidence that combining radiotherapy with ICB can unlock the mechanisms that drive resistance in patients; however, the sequencing of these therapies is still debated. This review offers a comprehensive overview of clinical trials and preclinical models that use radiotherapy-immunotherapy combinations in RC in an attempt to extrapolate the ideal sequencing of the two treatment modalities. The results highlight the dearth of evidence to answer the question of whether ICB should be given before, during, or after radiotherapy, yet it is suggested that improving the relevance of our preclinical models will provide a platform with higher translational value and will lead to appropriate clinical trial designs.
    Language English
    Publishing date 2023-12-02
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 3032799-4
    ISSN 2590-017X ; 2666-2345
    ISSN (online) 2590-017X
    ISSN 2666-2345
    DOI 10.36401/JIPO-23-10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Noninvasive Stratification of Colon Cancer by Multiplex PET Imaging.

    Malviya, Gaurav / Lannagan, Tamsin R M / Johnson, Emma / Mackintosh, Agata / Bielik, Robert / Peters, Adam / Soloviev, Dmitry / Brown, Gavin / Jackstadt, Rene / Nixon, Colin / Gilroy, Kathryn / Campbell, Andrew / Sansom, Owen J / Lewis, David Y

    Clinical cancer research : an official journal of the American Association for Cancer Research

    2024  Volume 30, Issue 8, Page(s) 1518–1529

    Abstract: ... four different metabolic PET tracers, [18F]fluorodeoxy-D-glucose ([18F]FDG), O-(2-[18F]fluoroethyl)-l ...

    Abstract Purpose: The current approach for molecular subtyping of colon cancer relies on gene expression profiling, which is invasive and has limited ability to reveal dynamics and spatial heterogeneity. Molecular imaging techniques, such as PET, present a noninvasive alternative for visualizing biological information from tumors. However, the factors influencing PET imaging phenotype, the suitable PET radiotracers for differentiating tumor subtypes, and the relationship between PET phenotypes and tumor genotype or gene expression-based subtyping remain unknown.
    Experimental design: In this study, we conducted 126 PET scans using four different metabolic PET tracers, [18F]fluorodeoxy-D-glucose ([18F]FDG), O-(2-[18F]fluoroethyl)-l-tyrosine ([18F]FET), 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT), and [11C]acetate ([11C]ACE), using a spectrum of five preclinical colon cancer models with varying genetics (BMT, AKPN, AK, AKPT, KPN), at three sites (subcutaneous, orthograft, autochthonous) and at two tumor stages (primary vs. metastatic).
    Results: The results demonstrate that imaging signatures are influenced by genotype, tumor environment, and stage. PET imaging signatures exhibited significant heterogeneity, with each cancer model displaying distinct radiotracer profiles. Oncogenic Kras and Apc loss showed the most distinctive imaging features, with [18F]FLT and [18F]FET being particularly effective, respectively. The tissue environment notably impacted [18F]FDG uptake, and in a metastatic model, [18F]FET demonstrated higher uptake.
    Conclusions: By examining factors contributing to PET-imaging phenotype, this study establishes the feasibility of noninvasive molecular stratification using multiplex radiotracer PET. It lays the foundation for further exploration of PET-based subtyping in human cancer, thereby facilitating noninvasive molecular diagnosis.
    MeSH term(s) Humans ; Fluorodeoxyglucose F18 ; Dideoxynucleosides ; Positron-Emission Tomography/methods ; Colonic Neoplasms/diagnostic imaging ; Colonic Neoplasms/genetics ; Radiopharmaceuticals
    Chemical Substances Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; alovudine (PG53R0DWDQ) ; Dideoxynucleosides ; Radiopharmaceuticals
    Language English
    Publishing date 2024-03-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1225457-5
    ISSN 1557-3265 ; 1078-0432
    ISSN (online) 1557-3265
    ISSN 1078-0432
    DOI 10.1158/1078-0432.CCR-23-1063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: RNA helicase EIF4A1-mediated translation is essential for the GC response.

    Screen, Michael / Matheson, Louise S / Howden, Andrew Jm / Strathdee, Douglas / Willis, Anne E / Bushell, Martin / Sansom, Owen / Turner, Martin

    Life science alliance

    2023  Volume 7, Issue 2

    Abstract: EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5' untranslated regions. However, very little is known of their roles ... ...

    Abstract EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5' untranslated regions. However, very little is known of their roles in nonmalignant cells. Using mouse models to delete
    MeSH term(s) Animals ; Mice ; Eukaryotic Initiation Factor-4A/genetics ; Eukaryotic Initiation Factor-4A/metabolism ; RNA Helicases/metabolism ; B-Lymphocytes
    Chemical Substances Eukaryotic Initiation Factor-4A (EC 2.7.7.-) ; RNA Helicases (EC 3.6.4.13)
    Language English
    Publishing date 2023-11-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202302301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Microenvironmental cues in cancer stemness.

    Flanagan, D J / Hodder, M C / Sansom, O J

    Nature cell biology

    2018  Volume 20, Issue 10, Page(s) 1102–1104

    MeSH term(s) Colonic Neoplasms ; Cues ; Humans ; Neoplastic Stem Cells ; Tumor Microenvironment
    Language English
    Publishing date 2018-09-26
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-018-0210-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Pre-clinical modelling of rectal cancer to develop novel radiotherapy-based treatment strategies.

    Gillespie, Michael A / Steele, Colin W / Lannagan, Tamsin R M / Sansom, Owen J / Roxburgh, Campbell S D

    Oncology reviews

    2021  Volume 15, Issue 1, Page(s) 511

    Abstract: Pre-operative chemoradiotherapy reduces local recurrence rates in locally advanced rectal cancer. 10-20% of patients undergo complete response to chemoradiotherapy, however, many patients show no response. The mechanisms underlying this are poorly ... ...

    Abstract Pre-operative chemoradiotherapy reduces local recurrence rates in locally advanced rectal cancer. 10-20% of patients undergo complete response to chemoradiotherapy, however, many patients show no response. The mechanisms underlying this are poorly understood; identifying molecular and immunological factors underpinning heterogeneous responses to chemoradiotherapy, will promote development of treatment strategies to improve responses and overcome resistance mechanisms. This review describes the advances made in pre-clinical modelling of colorectal cancer, including genetically engineered mouse models, transplantation models, patient derived organoids and radiotherapy platforms to study responses to chemoradiotherapy. Relevant literature was identified through the PubMed and MEDLINE databases, using the following keywords: rectal cancer; mouse models; organoids; neo-adjuvant treatment; radiotherapy; chemotherapy. By delineating the advantages and disadvantages of available models, we discuss how modelling techniques can be utilized to address current research priorities in locally advanced rectal cancer. We provide unique insight into the potential application of pre-clinical models in the development of novel neo-adjuvant treatment strategies, which will hopefully guide future clinical trials.
    Language English
    Publishing date 2021-06-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2390302-8
    ISSN 1970-5565 ; 1970-5565 ; 1970-5557
    ISSN (online) 1970-5565
    ISSN 1970-5565 ; 1970-5557
    DOI 10.4081/oncol.2021.511
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Comparison of Daily versus Admission and Discharge Surveillance Cultures for Multidrug-Resistant Organism Detection in an Intensive Care Unit.

    Sansom, Sarah E / Shimasaki, Teppei / Dangana, Thelma / Lin, Michael Y / Schoeny, Michael E / Fukuda, Christine / Moore, Nicholas M / Yelin, Rachel D / Bassis, Christine M / Rhee, Yoona / Cornejo Cisneros, Enrique / Bell, Pamela / Lolans, Karen / Aboushaala, Khaled / Young, Vincent B / Hayden, Mary K

    The Journal of infectious diseases

    2024  

    Abstract: Background: Admission and discharge screening of patients for asymptomatic gut colonization with multidrug-resistant organisms (MDROs) is a traditional approach to active surveillance, but its sensitivity for detecting colonization is uncertain.: ... ...

    Abstract Background: Admission and discharge screening of patients for asymptomatic gut colonization with multidrug-resistant organisms (MDROs) is a traditional approach to active surveillance, but its sensitivity for detecting colonization is uncertain.
    Methods: Daily rectal or fecal swab samples and clinical data were collected over 12 months from patients in one 25-bed intensive care unit (ICU) in Chicago, IL USA and tested for the following multidrug-resistant organisms (MDROs): vancomycin-resistant enterococci (VRE); third-generation cephalosporin-resistant Enterobacterales, including extended-spectrum β-lactamase-producing Enterobacterales (ESBL); and carbapenem-resistant Enterobacterales (CRE). MDRO detection by (1) admission/discharge surveillance cultures or (2) clinical cultures were compared to daily surveillance cultures. Samples underwent 16S rRNA gene sequencing to measure the relative abundance of operational taxonomic units (OTUs) corresponding to each MDRO.
    Results: Compared with daily surveillance cultures, admission/discharge cultures detected 91% of prevalent MDRO colonization and 63% of incident MDRO colonization among medical ICU patients. Only a minority (7%) of MDRO carriers were identified by clinical cultures. Higher relative abundance of MDRO-associated OTUs and specific antibiotic exposures were independently associated with higher probability of MDRO detection by culture.
    Conclusion: Admission and discharge surveillance cultures underestimated MDRO acquisitions in an ICU. These limitations should be considered when designing sampling strategies for epidemiologic studies that use culture-based surveillance.
    Language English
    Publishing date 2024-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiae162
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Concerns About a Dog Model of Dry Eye Disease.

    Ofri, Ron / Millichamp, Nicholas J / Keller, Charlotte / McLellan, Gillian J / Komáromy, András M / Morton, David / Matas, Màrian / Michau, Tammy M / Coall, Sarah / Sansom, Jane / Leonard, Brian C

    Translational vision science & technology

    2024  Volume 13, Issue 3, Page(s) 28

    MeSH term(s) Dogs ; Animals ; Dry Eye Syndromes/diagnosis ; Tears
    Language English
    Publishing date 2024-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2674602-5
    ISSN 2164-2591 ; 2164-2591
    ISSN (online) 2164-2591
    ISSN 2164-2591
    DOI 10.1167/tvst.13.3.28
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