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Article: [Meta-analysis on effectiveness and safety of Pudilan Xiaoyan Oral Liquid on child upper respiratory infection].

Bai, Yu / Li, Yu-Xiang / Shi, Yu-Jing / Zhao, Hai-Yu

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2020 Volume 45, Issue 9, Page(s) 2203–2209

Abstract: To systematically review the effectiveness and safety of Pudilan Xiaoyan Oral Liquid on child ... VIP, WanFang and CNKI, for published articles of randomized controlled trials(RCTs) of Pudilan Xiaoyan ... evidence shows that Pudilan Xiaoyan Oral Liquid may significantly improve the effectiveness and safety ...

Abstract	To systematically review the effectiveness and safety of Pudilan Xiaoyan Oral Liquid on child upper respiratory infection and conduct Meta-analysis. We electronically retrieved databases, including PubMed, Web of Science, VIP, WanFang and CNKI, for published articles of randomized controlled trials(RCTs) of Pudilan Xiaoyan Oral Liquid on child upper respiratory infection from inception to April 2019. According to the inclusion and exclusion criteria, two reviewers independently screened out literatures, extracted data and assessed the risk of bias in included studies. Then, Meta-analysis were conducted by Stata 15.0 software. A total of 16 RCTs involving 1 924 patients with upper respiratory infection were included. The results of Meta-analysis showed that the improvement of clinical symptoms, such as fever subsided time(WMD=-3.66, 95%CI[-4.61,-2.72], P<0.001), cough time(WMD=-1.89, 95%CI[-2.51,-1.27], P<0.001), time of runny noses(WMD=-4.60, 95%CI[-5.85,-3.34], P<0.001) and time of sore throat(WMD=-2.62, 95%CI[-3.54,-1.70], P<0.001). Meanwhile, the results of Meta-analysis showed the improvement of laboratory indications, including TNF-α(WMD=-2.68, 95%CI[-2.98,-1.58], P<0.001) and IL-6(WMD=-2.26, 95%CI[-3.36,-2.36], P<0.01). The current evidence shows that Pudilan Xiaoyan Oral Liquid may significantly improve the effectiveness and safety. According to the limited quality of included studies, the above conclusion needs be to verified with more high-quality studies.
MeSH term(s)	Child ; Drugs, Chinese Herbal ; Humans ; Pharyngitis ; Tumor Necrosis Factor-alpha
Chemical Substances	Drugs, Chinese Herbal ; Pudilan xiaoyan ; Tumor Necrosis Factor-alpha
Language	Chinese
Publishing date	2020-06-03
Publishing country	China
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20200102.501
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [Anti-inflammatory mechanism of qingfei xiaoyan wan studied with network pharmacology].

Cheng, Bin-Feng / Hou, Yuan-Yuan / Jiang, Min / Zhao, Zhen-Ying / Dong, Lin-Yi / Bai, Gang

Yao xue xue bao = Acta pharmaceutica Sinica

2013 Volume 48, Issue 5, Page(s) 686–693

Abstract: This study aims to clarify out the anti-inflammatory mechanism of Qingfei Xiaoyan Wan. Chemical ... constituents of Qingfei Xiaoyan Wan identified by UPLC Q-TOF, were submit to Molinspiration, PharmMapper and ... through 11 protein targets; Qingfei Xiaoyan Wan treatment can significantly reduce the infiltration ...

Abstract	This study aims to clarify out the anti-inflammatory mechanism of Qingfei Xiaoyan Wan. Chemical constituents of Qingfei Xiaoyan Wan identified by UPLC Q-TOF, were submit to Molinspiration, PharmMapper and KEGG bioinformatics softwares for predicting their absorption parameters, target proteins and related pathways respectively; and the gene chip and real time-PCR were carried out to investigate the expression of inflammatory genes on lung tissue of guinea pigs or human bronchial epithelial cell lines. The predicted results showed that 19 of the 24 absorbable constituents affected at 9 inflammation-related pathways through 11 protein targets; Qingfei Xiaoyan Wan treatment can significantly reduce the infiltration of cytokines through ERK1 gene and 5 inflammatory pathways (Focal adhesion, Fc epsilon RI, Toll-like receptors, NK cell-mediated cytotoxic, and ERK/MAPK). The results of real time-PCR further confirmed that the anti-inflammatory effects of Qingfei Xiaoyan Wan were due to active ingredients such as arctigenin, cholic acid and sinapic acid intervened focal adhesion, Fc epsilon RI signaling and ERK/MAPK pathways. The novel approach of 'drug-target-pathway' will present an effective strategy for the study of traditional Chinese medicines.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Asthma/metabolism ; Asthma/pathology ; Cell Line ; Cholic Acid/pharmacology ; Coumaric Acids/pharmacology ; Cytokines/metabolism ; Drug Combinations ; Drugs, Chinese Herbal/pharmacology ; Epithelial Cells/drug effects ; Female ; Furans/pharmacology ; Guinea Pigs ; Humans ; Inflammation/metabolism ; Lignans/pharmacology ; Lung/pathology ; MAP Kinase Signaling System ; Male ; Random Allocation ; Receptors, IgE/metabolism ; Toll-Like Receptors/metabolism
Chemical Substances	Anti-Inflammatory Agents ; Coumaric Acids ; Cytokines ; Drug Combinations ; Drugs, Chinese Herbal ; Furans ; Lignans ; Receptors, IgE ; Toll-Like Receptors ; sinapinic acid (68A28V6010) ; Cholic Acid (G1JO7801AE) ; arctigenin (U76MR9VS6M)
Language	Chinese
Publishing date	2013-05
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	788758-9
ISSN	0513-4870
ISSN	0513-4870
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Article ; Online: Qingfei Xiaoyan Wan alleviates asthma through multi-target network regulation.

Zhao, Zhenying / Miao, Yingbo / Pan, Pengwei / Cheng, Binfeng / Bai, Gang / Wu, Hong

BMC complementary and alternative medicine

2013 Volume 13, Page(s) 206

Abstract: Background: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used ...

Abstract	Background: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving cough, asthma, upper respiratory tract infection, bronchitis, pneumonia, and etc. in clinic. Comparing with other anti-asthma drugs, it is characterised with moderate and persistent efficacy as well as few side effects, however, the underlying action mechanism still remains elusive. This study aimed to identify QFXY multi-target network regulation as an asthma controller. Methods: This study established asthma model induced by histamine phosphate and acetylcholine chloride (His&Ach) in guinea pigs, which then were administered orally with QFXY. Hematoxylin-Eosin staining sections were applied for evaluating QFXY effect. In both Model and QFXY groups, customized microarrays and 2D electrophoresis were adopted to detect differentially expressed genes (diff genes) and proteins (diff proteins) respectively, and some diff proteins were identified with MALDI-TOF/MS. The checked diff genes and proteins underwent Cluster, GO and KEGG analysis. Based on GAD and HPRD databases, QFXY-asthma target regulation network was constructed. Results: His&Ach-induced asthma model of guinea pigs was established. HE sections presented anti-inflammation and anti-remodelling effects of QFXY. Comparing with the Model group, 55 diff genes and 6 diff proteins were identified in QFXY group. Validation by qPCR and Western blot showed the microarray and 2D data reliable. Furthermore, QFXY-asthma target regulation network was achieved. Conclusions: A primarily combined genomic and proteomic screening of QFXY targets displayed a series of candidate genes and proteins, which indicated that the effect of QFXY relied on the combined mechanism, anti-inflammation and anti-remodelling, as well as influencing signal transduction in vivo.
MeSH term(s)	Acetylcholine/toxicity ; Animals ; Asthma/chemically induced ; Asthma/genetics ; Asthma/metabolism ; Asthma/therapy ; Cluster Analysis ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Electrophoresis, Gel, Two-Dimensional ; Female ; Gene Expression/drug effects ; Gene Regulatory Networks/genetics ; Gene Regulatory Networks/physiology ; Guinea Pigs ; Histamine/analogs & derivatives ; Histamine/toxicity ; Lung/chemistry ; Lung/drug effects ; Lung/metabolism ; Lung/pathology ; Male ; Oligonucleotide Array Sequence Analysis ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	Drugs, Chinese Herbal ; Histamine (820484N8I3) ; Acetylcholine (N9YNS0M02X) ; histamine phosphate (QWB37T4WZZ)
Language	English
Publishing date	2013-08-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-13-206
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of Qingfei Xiaoyan pill combined with ceftriaxone and azithromycin on immune index, oxidative stress and inflammatory index in senile CAP patients

Xiao-Rui Bai / Le Gao / Li Li

Journal of Hainan Medical University, Vol 25, Iss 10, Pp 36-

2019 Volume 39

Abstract: Objective: To explore the effects of Qingfei Xiaoyan pill combined with ceftriaxone and ... with ceftriaxone combined with azithromycin, while the observation group was treated with Qingfei Xiaoyan Pill ... significantly lower than those in the control group. Conclusions: Qingfei Xiaoyan pill combined with ceftriaxone ...

Abstract	Objective: To explore the effects of Qingfei Xiaoyan pill combined with ceftriaxone and azithromycin on immune index, oxidative stress and inflammatory index in senile CAP patients. Methods: A total of 130 patients with CAP admitted to Yulin first hospital from October 2016 to December 2017 were randomly divided into control group (n = 65) and observation group (n = 65), the control group was treated with ceftriaxone combined with azithromycin, while the observation group was treated with Qingfei Xiaoyan Pill on the basis of the control group, the changes of cellular humoral immune function, oxidative stress index and inflammatory factors were compared between the two groups before and after treatment. Results: After treatment, the levels of CD4+ and CD4+/CD8+ in both groups were significantly higher than before treatment, while the level of CD8+ was significantly lower than before treatment, the levels of CD4+ and CD4+/CD8+ in the observation group were significantly higher than those in the control group, while the level of CD8+ was significantly lower than those in the control group; the levels of IgA and IgM in the observation group were significantly higher than those in the control group; the levels of MDA and SOD in the observation group were significantly lower than those in the control group, and the level of MDA in the observation group was significantly lower than that in the control group, the levels of SOD in the observation group were significantly higher than those in the control group; the levels of PCT and CRP in the two groups were significantly lower than those before treatment , and the levels of PCT and CRP in the observation group were significantly lower than those in the control group. Conclusions: Qingfei Xiaoyan pill combined with ceftriaxone and azithromycin can effectively enhance the immune function of elderly patients with CAP, enhance the body's antioxidant effect and reduce its inflammatory reaction, which is of clinical significance.
Keywords	qingfei xiaoyan pill ; cap ; immunity ; oxidative stress ; inflammation ; Medicine ; R
Subject code	630
Language	English
Publishing date	2019-05-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Searching for synergistic bronchodilators and novel therapeutic regimens for chronic lung diseases from a traditional Chinese medicine, Qingfei Xiaoyan Wan.

Hou, Yuanyuan / Cheng, Binfeng / Zhou, Mengge / Fang, Runping / Jiang, Min / Hou, Wenbin / Bai, Gang

PloS one

2014 Volume 9, Issue 11, Page(s) e113104

Abstract: ... A classical formula known as Qingfei Xiaoyan Wan, used clinically as a treatment for prevalent chronic ...

Abstract	Classical Chinese pharmacopeias describe numerous excellent herbal formulations, and each prescription is an outstanding pool of effective compounds for drug discovery. Clarifying the bioactivity of the combined mechanisms of the ingredients in complex traditional Chinese medicine formulas is challenging. A classical formula known as Qingfei Xiaoyan Wan, used clinically as a treatment for prevalent chronic lung disease, was investigated in this work. A mutually enhanced bioactivity-guided ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) characterization system was proposed, coupled with a dual-luciferase reporter assay for β2AR-agonist cofactor screening. Arctiin, arctigenin, descurainoside and descurainolide B, four lignin compounds that showed synergistic bronchodilation effects with ephedrine, were revealed. The synergistic mechanism of arctigenin with the β2ARagonist involved with the reduction of free Ca2+ was clarified by a dual-luciferase reporter assay for intracellular calcium and the Ca2+ indicator fluo-4/AM to monitor changes in the fluorescence. The relaxant and contractile responses of airway smooth muscle are regulated by crosstalk between the intracellular cAMP and calcium signaling pathways. Our data indicated the non-selective βAR agonist ephedrine as the principal bronchodilator of the formula, whereas the lignin ingredients served as adjuvant ingredients. A greater understanding of the mechanisms governing the control of these pathways, based on conventional wisdom, could lead to the identification of novel therapeutic targets or new agents for the treatment of asthma and COPD.
MeSH term(s)	Adrenergic beta-2 Receptor Agonists/pharmacology ; Adrenergic beta-2 Receptor Agonists/therapeutic use ; Animals ; Asthma/chemically induced ; Asthma/drug therapy ; Bronchodilator Agents/pharmacology ; Bronchodilator Agents/therapeutic use ; Calcium/metabolism ; Cell Line ; Chromatography, High Pressure Liquid ; Chronic Disease ; Disease Models, Animal ; Drug Synergism ; Drugs, Chinese Herbal/analysis ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Ephedrine/pharmacology ; Ephedrine/therapeutic use ; Flavonoids/isolation & purification ; Flavonoids/pharmacology ; Flavonoids/therapeutic use ; Furans/isolation & purification ; Furans/pharmacology ; Furans/therapeutic use ; Glucosides/isolation & purification ; Glucosides/pharmacology ; Glucosides/therapeutic use ; Guinea Pigs ; Humans ; Lactones/isolation & purification ; Lactones/pharmacology ; Lactones/therapeutic use ; Lignans/isolation & purification ; Lignans/pharmacology ; Lignans/therapeutic use ; Lung Diseases/drug therapy ; Medicine, Chinese Traditional ; Myocytes, Smooth Muscle/cytology ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism ; Trachea/drug effects ; Trachea/physiopathology
Chemical Substances	Adrenergic beta-2 Receptor Agonists ; Bronchodilator Agents ; Drugs, Chinese Herbal ; Flavonoids ; Furans ; Glucosides ; Lactones ; Lignans ; descurainolide B ; descurainoside B ; Ephedrine (GN83C131XS) ; Calcium (SY7Q814VUP) ; arctiin (TM5RQ949K7) ; arctigenin (U76MR9VS6M)
Language	English
Publishing date	2014-11-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0113104
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Qingfei Xiaoyan Wan alleviates asthma through multi-target network regulation

Zhao, Zhenying / Miao, Yingbo / Pan, Pengwei / Cheng, Binfeng / Bai, Gang / Wu, Hong

BMC Complement Altern Med. 2013 Dec., v. 13, no. 1 p.206-206

2013

Abstract: BACKGROUND: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving ...

Abstract	BACKGROUND: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving cough, asthma, upper respiratory tract infection, bronchitis, pneumonia, and etc. in clinic. Comparing with other anti-asthma drugs, it is characterised with moderate and persistent efficacy as well as few side effects, however, the underlying action mechanism still remains elusive. This study aimed to identify QFXY multi-target network regulation as an asthma controller. METHODS: This study established asthma model induced by histamine phosphate and acetylcholine chloride (His&Ach) in guinea pigs, which then were administered orally with QFXY. Hematoxylin-Eosin staining sections were applied for evaluating QFXY effect. In both Model and QFXY groups, customized microarrays and 2D electrophoresis were adopted to detect differentially expressed genes (diff genes) and proteins (diff proteins) respectively, and some diff proteins were identified with MALDI-TOF/MS. The checked diff genes and proteins underwent Cluster, GO and KEGG analysis. Based on GAD and HPRD databases, QFXY-asthma target regulation network was constructed. RESULTS: His&Ach-induced asthma model of guinea pigs was established. HE sections presented anti-inflammation and anti-remodelling effects of QFXY. Comparing with the Model group, 55 diff genes and 6 diff proteins were identified in QFXY group. Validation by qPCR and Western blot showed the microarray and 2D data reliable. Furthermore, QFXY-asthma target regulation network was achieved. CONCLUSIONS: A primarily combined genomic and proteomic screening of QFXY targets displayed a series of candidate genes and proteins, which indicated that the effect of QFXY relied on the combined mechanism, anti-inflammation and anti-remodelling, as well as influencing signal transduction in vivo.
Keywords	Western blotting ; acetylcholine ; asthma ; bronchitis ; chlorides ; complement ; cough ; electrophoresis ; gene expression regulation ; genomics ; histamine ; microarray technology ; models ; phosphates ; pneumonia ; proteomics ; signal transduction
Language	English
Dates of publication	2013-12
Size	p. 206.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2050429-9
ISSN	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-13-206
Database	NAL-Catalogue (AGRICOLA)

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Article: Qingfei Xiaoyan Wan, a traditional Chinese medicine formula, ameliorates Pseudomonas aeruginosa-induced acute lung inflammation by regulation of PI3K/AKT and Ras/MAPK pathways.

Hou, Yuanyuan / Nie, Yan / Cheng, Binfeng / Tao, Jin / Ma, Xiaoyao / Jiang, Min / Gao, Jie / Bai, Gang

Acta pharmaceutica Sinica. B

2016 Volume 6, Issue 3, Page(s) 212–221

Abstract: ... to global public health. Qingfei Xiaoyan Wan (QF), a traditional Chinese medicine (TCM) formula, has been ...

Abstract	Gram-negative pathogen-induced nosocomial infections and resistance are a most serious menace to global public health. Qingfei Xiaoyan Wan (QF), a traditional Chinese medicine (TCM) formula, has been used clinically in China for the treatment of upper respiratory tract infections, acute or chronic bronchitis and pulmonary infection. In this study, the effects of QF on Pseudomonas aeruginosa-induced acute pneumonia in mice were evaluated. The mechanisms by which four typical anti-inflammatory ingredients from QF, arctigenin (ATG), cholic acid (CLA), chlorogenic acid (CGA) and sinapic acid (SPA), regulate anti-inflammatory signaling pathways and related targets were investigated using molecular biology and molecular docking techniques. The results showed that pretreatment with QF significantly inhibits the release of cytokines (TNF-α and IL-6) and chemokines (IL-8 and RANTES), reduces leukocytes recruitment into inflamed tissues and ameliorates pulmonary edema and necrosis. In addition, ATG was identified as the primary anti-inflammatory agent with action on the PI3K/AKT and Ras/MAPK pathways. CLA and CGA enhanced the actions of ATG and exhibited synergistic NF-κB inactivation effects possibly via the Ras/MAPK signaling pathway. Moreover, CLA is speculated to target FGFR and MEK firstly. Overall, QF regulated the PI3K/AKT and Ras/MAPK pathways to inhibit pathogenic bacterial infections effectively.
Keywords	covid19
Language	English
Publishing date	2016-03-22
Publishing country	Netherlands
Document type	Journal Article
ISSN	2211-3835
ISSN	2211-3835
DOI	10.1016/j.apsb.2016.03.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Searching for synergistic bronchodilators and novel therapeutic regimens for chronic lung diseases from a traditional Chinese medicine, Qingfei Xiaoyan Wan.

Yuanyuan Hou / Binfeng Cheng / Mengge Zhou / Runping Fang / Min Jiang / Wenbin Hou / Gang Bai

PLoS ONE, Vol 9, Iss 11, p e

2014 Volume 113104

Abstract: ... A classical formula known as Qingfei Xiaoyan Wan, used clinically as a treatment for prevalent chronic ...

Abstract	Classical Chinese pharmacopeias describe numerous excellent herbal formulations, and each prescription is an outstanding pool of effective compounds for drug discovery. Clarifying the bioactivity of the combined mechanisms of the ingredients in complex traditional Chinese medicine formulas is challenging. A classical formula known as Qingfei Xiaoyan Wan, used clinically as a treatment for prevalent chronic lung disease, was investigated in this work. A mutually enhanced bioactivity-guided ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) characterization system was proposed, coupled with a dual-luciferase reporter assay for β2AR-agonist cofactor screening. Arctiin, arctigenin, descurainoside and descurainolide B, four lignin compounds that showed synergistic bronchodilation effects with ephedrine, were revealed. The synergistic mechanism of arctigenin with the β2ARagonist involved with the reduction of free Ca2+ was clarified by a dual-luciferase reporter assay for intracellular calcium and the Ca2+ indicator fluo-4/AM to monitor changes in the fluorescence. The relaxant and contractile responses of airway smooth muscle are regulated by crosstalk between the intracellular cAMP and calcium signaling pathways. Our data indicated the non-selective βAR agonist ephedrine as the principal bronchodilator of the formula, whereas the lignin ingredients served as adjuvant ingredients. A greater understanding of the mechanisms governing the control of these pathways, based on conventional wisdom, could lead to the identification of novel therapeutic targets or new agents for the treatment of asthma and COPD.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Qingfei Xiaoyan Wan, a traditional Chinese medicine formula, ameliorates Pseudomonas aeruginosa–induced acute lung inflammation by regulation of PI3K/AKT and Ras/MAPK pathways

Yuanyuan Hou / Yan Nie / Binfeng Cheng / Jin Tao / Xiaoyao Ma / Min Jiang / Jie Gao / Gang Bai

Acta Pharmaceutica Sinica B, Vol 6, Iss 3, Pp 212-

2016 Volume 221

Abstract: ... to global public health. Qingfei Xiaoyan Wan (QF), a traditional Chinese medicine (TCM) formula, has been ...

Abstract	Gram-negative pathogen–induced nosocomial infections and resistance are a most serious menace to global public health. Qingfei Xiaoyan Wan (QF), a traditional Chinese medicine (TCM) formula, has been used clinically in China for the treatment of upper respiratory tract infections, acute or chronic bronchitis and pulmonary infection. In this study, the effects of QF on Pseudomonas aeruginosa–induced acute pneumonia in mice were evaluated. The mechanisms by which four typical anti-inflammatory ingredients from QF, arctigenin (ATG), cholic acid (CLA), chlorogenic acid (CGA) and sinapic acid (SPA), regulate anti-inflammatory signaling pathways and related targets were investigated using molecular biology and molecular docking techniques. The results showed that pretreatment with QF significantly inhibits the release of cytokines (TNF-α and IL-6) and chemokines (IL-8 and RANTES), reduces leukocytes recruitment into inflamed tissues and ameliorates pulmonary edema and necrosis. In addition, ATG was identified as the primary anti-inflammatory agent with action on the PI3K/AKT and Ras/MAPK pathways. CLA and CGA enhanced the actions of ATG and exhibited synergistic NF-κB inactivation effects possibly via the Ras/MAPK signaling pathway. Moreover, CLA is speculated to target FGFR and MEK firstly. Overall, QF regulated the PI3K/AKT and Ras/MAPK pathways to inhibit pathogenic bacterial infections effectively.
Keywords	Anti-inflammatory ; Network pharmacology ; Pathogenic bacterial infection ; PI3K/AKT pathway ; Ras/MAPK pathway ; Lung ; Mouse ; Medicine ; R ; Therapeutics. Pharmacology ; RM1-950
Subject code	570
Publishing date	2016-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dual-bioactivity-based liquid chromatography-coupled quadrupole time-of-flight mass spectrometry for NF-κB inhibitors and β2AR agonists identification in Chinese Medicinal Preparation Qingfei Xiaoyan Wan.

Cheng, Binfeng / Hou, Yuanyuan / Wang, Liqiang / Dong, Linyi / Peng, Jiamin / Bai, Gang

Analytical and bioanalytical chemistry

2012 Volume 404, Issue 8, Page(s) 2445–2452

Abstract: ... preparations. Qingfei Xiaoyan Wan, a TCM preparation used for the clinical therapy of asthma, was ...

Abstract	Traditional Chinese medicine (TCM) preparations have been used as an effective multitarget strategy for the treatment of complex diseases; however, their bioactive constituents are undefined and difficult to identify. In this study, a simple and dual-target method based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry combined with dual-bioactive (NF-κB and β(2)-adrenergic receptor) luciferase reporter assay systems was developed for the rapid determination of various bioactive compounds of TCM preparations. Qingfei Xiaoyan Wan, a TCM preparation used for the clinical therapy of asthma, was analyzed with this method. Potential anti-inflammatory and spasmolytic constituents were screened using NF-κB and β(2)-adrenergic receptor activity luciferase reporter assay systems and simultaneously identified according to the time-of-flight mass spectrometry data. One β(2)-adrenergic receptor agonist (ephedrine) and four structural types of NF-κB inhibitors (arctigenin derivatives, cholic acid derivatives, chlorogenic acid, and sinapic acid) were characterized. Tracheloside was considered a new NF-κB inhibitor. Further cytokine and chemokine detection confirmed the anti-inflammatory effects of the potential NF-κB inhibitors. The integration of ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and dual-bioactive human cell functional evaluation systems proved to be a simple and effective strategy for the rapid screening of various bioactive compounds in TCM preparations used to treat complex diseases.
MeSH term(s)	Adrenergic beta-2 Receptor Agonists/analysis ; Adrenergic beta-2 Receptor Agonists/chemistry ; Cell Line ; Chromatography, Liquid ; Drug Delivery Systems ; Drugs, Chinese Herbal/analysis ; Drugs, Chinese Herbal/chemistry ; Humans ; Molecular Structure ; NF-kappa B/antagonists & inhibitors ; Tandem Mass Spectrometry ; Time Factors
Chemical Substances	Adrenergic beta-2 Receptor Agonists ; Drugs, Chinese Herbal ; NF-kappa B
Language	English
Publishing date	2012-11
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-012-6332-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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