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Article ; Online: MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability.

Liu, Jin-Xin / Chen, Ao-Nan / Yu, Qihong / Shi, Ke-Tai / Liu, Yi-Bo / Guo, Cui-Lian / Wang, Zhe-Zheng / Yao, Yin / Pan, Li / Lu, Xiang / Xu, Kai / Wang, Heng / Zeng, Ming / Liu, Chaohong / Schleimer, Robert P / Wu, Ning / Liao, Bo / Liu, Zheng

JCI insight

2023 Volume 8, Issue 9

Abstract: Although the expression of Mex3 RNA-binding family member B (MEX3B) is upregulated in human nasal epithelial cells (HNECs) predominately in the eosinophilic chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) subtype, its functions as an RNA binding ... ...

Abstract	Although the expression of Mex3 RNA-binding family member B (MEX3B) is upregulated in human nasal epithelial cells (HNECs) predominately in the eosinophilic chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) subtype, its functions as an RNA binding protein in airway epithelial cells remain unknown. Here, we revealed the role of MEX3B based on different subtypes of CRS and demonstrated that MEX3B decreased the TGF-β receptor III (TGFBR3) mRNA level by binding to its 3' UTR and reducing its stability in HNECs. TGF-βR3 was found to be a TGF-β2-specific coreceptor in HNECs. Knocking down or overexpressing MEX3B promoted or inhibited TGF-β2-induced phosphorylation of SMAD2 in HNECs, respectively. TGF-βR3 and phosphorylated SMAD2 levels were downregulated in CRSwNP compared with controls and CRS without nasal polyps with a more prominent downregulation in the eosinophilic CRSwNP. TGF-β2 promoted collagen production in HNECs. Collagen abundance decreased and edema scores increased in CRSwNP compared with control, again more prominently in the eosinophilic type. Collagen expression in eosinophilic CRSwNP was negatively correlated with MEX3B but positively correlated with TGF-βR3. These results suggest that MEX3B inhibits tissue fibrosis in eosinophilic CRSwNP by downregulating epithelial cell TGFBR3 expression; consequently, MEX3B might be a valuable therapeutic target against eosinophilic CRSwNP.
MeSH term(s)	Humans ; Rhinitis/complications ; Rhinitis/metabolism ; Nasal Polyps/genetics ; Nasal Polyps/metabolism ; Transforming Growth Factor beta2/metabolism ; Sinusitis/genetics ; Sinusitis/metabolism ; Receptors, Transforming Growth Factor beta/genetics ; Receptors, Transforming Growth Factor beta/metabolism ; Epithelial Cells/metabolism ; RNA-Binding Proteins/genetics
Chemical Substances	betaglycan (145170-29-2) ; Transforming Growth Factor beta2 ; Receptors, Transforming Growth Factor beta ; MEX3B protein, human ; RNA-Binding Proteins
Language	English
Publishing date	2023-05-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.159058
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Article ; Online: MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability

Jin-Xin Liu / Ao-Nan Chen / Qihong Yu / Ke-Tai Shi / Yi-Bo Liu / Cui-Lian Guo / Zhe-Zheng Wang / Yin Yao / Li Pan / Xiang Lu / Kai Xu / Heng Wang / Ming Zeng / Chaohong Liu / Robert P. Schleimer / Ning Wu / Bo Liao / Zheng Liu

JCI Insight, Vol 8, Iss

2023 Volume 9

Abstract	Although the expression of Mex3 RNA-binding family member B (MEX3B) is upregulated in human nasal epithelial cells (HNECs) predominately in the eosinophilic chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) subtype, its functions as an RNA binding protein in airway epithelial cells remain unknown. Here, we revealed the role of MEX3B based on different subtypes of CRS and demonstrated that MEX3B decreased the TGF-β receptor III (TGFBR3) mRNA level by binding to its 3′ UTR and reducing its stability in HNECs. TGF-βR3 was found to be a TGF-β2–specific coreceptor in HNECs. Knocking down or overexpressing MEX3B promoted or inhibited TGF-β2–induced phosphorylation of SMAD2 in HNECs, respectively. TGF-βR3 and phosphorylated SMAD2 levels were downregulated in CRSwNP compared with controls and CRS without nasal polyps with a more prominent downregulation in the eosinophilic CRSwNP. TGF-β2 promoted collagen production in HNECs. Collagen abundance decreased and edema scores increased in CRSwNP compared with control, again more prominently in the eosinophilic type. Collagen expression in eosinophilic CRSwNP was negatively correlated with MEX3B but positively correlated with TGF-βR3. These results suggest that MEX3B inhibits tissue fibrosis in eosinophilic CRSwNP by downregulating epithelial cell TGFBR3 expression; consequently, MEX3B might be a valuable therapeutic target against eosinophilic CRSwNP.
Keywords	Inflammation ; Medicine ; R
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	American Society for Clinical investigation
Document type	Article ; Online
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Article: Endoplasmic reticulum stress promotes local immunoglobulin E production in allergic rhinitis.

Liu, Jin-Xin / Zhen, Zhen / Chen, Ao-Nan / Guo, Cui-Lian / Shi, Ke-Tai / Wang, Heng / Xu, Kai / Yao, Yin / Wang, Hai / Liao, Bo / Liu, Zheng

Laryngoscope investigative otolaryngology

2021 Volume 6, Issue 6, Page(s) 1256–1266

Abstract: Background: The role of endoplasmic reticulum (ER) stress in the pathogenesis of allergic rhinitis (AR) remains elusive.: Methods: Real-time polymerase chain reaction (RT-PCR), immunohistochemistry, and western blotting analyses were performed to ... ...

Abstract	Background: The role of endoplasmic reticulum (ER) stress in the pathogenesis of allergic rhinitis (AR) remains elusive. Methods: Real-time polymerase chain reaction (RT-PCR), immunohistochemistry, and western blotting analyses were performed to detect the expression of ER stress and unfolded protein response markers: 78-kDa glucose-regulated protein (GRP78), C/EBP homologous protein (CHOP), activating transcription factor 6 (ATF6α), spliced X-box binding protein 1 (sXBP-1), and phosphorylated eukaryotic initiation factor 2α ( Results: Compared to those in non-AR controls, the mRNA and protein levels of GRP78, CHOP, ATF6α, sXBP-1, and Conclusions: ER stress may be involved in the regulation of local IgE production in patients with AR. Inhibition of ER stress potentially provides a therapeutic avenue in AR by reducing local IgE production. Level of evidence: NA.
Language	English
Publishing date	2021-11-08
Publishing country	United States
Document type	Journal Article
ISSN	2378-8038
ISSN	2378-8038
DOI	10.1002/lio2.693
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Article ; Online: Design, Synthesis, and Fungicidal Activities of Novel

Ma, Yi-Dan / Zhou, Huan / Lin, Guo-Tai / Wu, Ke-Huan / Xu, Gong / Liu, Xili / Xu, Dan

Journal of agricultural and food chemistry

2024 Volume 72, Issue 12, Page(s) 6691–6701

Abstract: To accelerate the development of novel fungicides, a variety ... ...

Abstract	To accelerate the development of novel fungicides, a variety of
MeSH term(s)	Structure-Activity Relationship ; Antifungal Agents/pharmacology ; Diphenylamine/chemistry ; Molecular Docking Simulation ; Fungicides, Industrial/chemistry ; Benzamides ; Succinate Dehydrogenase ; Strobilurins ; Acetates ; Amides ; Imines
Chemical Substances	Antifungal Agents ; trifloxystrobin (F625Z36B2D) ; fluxapyroxad (7U8P4NAR2S) ; Diphenylamine (9N3CBB0BIQ) ; benzamide (6X80438640) ; Fungicides, Industrial ; Benzamides ; Succinate Dehydrogenase (EC 1.3.99.1) ; Strobilurins ; Acetates ; Amides ; Imines
Language	English
Publishing date	2024-03-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c07567
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Article ; Online: Fiber-reinforced gelatin/β-cyclodextrin hydrogels loaded with platelet-rich plasma-derived exosomes for diabetic wound healing.

Shu, Qiu-Hao / Zuo, Rong-Tai / Chu, Min / Shi, Jing-Jing / Ke, Qin-Fei / Guan, Jun-Jie / Guo, Ya-Ping

Biomaterials advances

2023 Volume 154, Page(s) 213640

Abstract: Diabetic complications with high-glucose status (HGS) cause the dysregulated autophagy and excessive apoptosis of multiple-type cells, leading to the difficulty in wound self-healing. Herein, we firstly developed fiber-reinforced gelatin (GEL)/β- ... ...

Abstract	Diabetic complications with high-glucose status (HGS) cause the dysregulated autophagy and excessive apoptosis of multiple-type cells, leading to the difficulty in wound self-healing. Herein, we firstly developed fiber-reinforced gelatin (GEL)/β-cyclodextrin (β-CD) therapeutic hydrogels by the modification of platelet-rich plasma exosomes (PRP-EXOs). The GEL fibers that were uniformly dispersed within the GEL/β-CD hydrogels remarkably enhanced the compression strengths and viscoelasticity. The PRP-EXOs were encapsulated in the hydrogels via the covalent crosslinking between the PRP-EXOs and genipin. The diabetic rat models demonstrated that the GEL/β-CD hydrogels and PRP-EXOs cooperatively promoted diabetic wound healing. On the one hand, the GEL/β-CD hydrogels provided the biocompatible microenvironments and active components for cell adhesion, proliferation and skin tissue regeneration. On the other hand, the PRP-EXOs in the therapeutic hydrogels significantly activated the autophagy and inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) and human skin fibroblasts (HSFs). The activation of autophagy and inhibition of apoptosis in HUVECs and HSFs induced the blood vessel creation, collagen formation and re-epithelialization. Taken together, this work proved that the incorporation of PRP-EXOs in a wound dressing was an effective strategy to regulate autophagy and apoptosis, and provide a novel therapeutic platform for diabetic wound healing.
MeSH term(s)	Rats ; Humans ; Animals ; Hydrogels/pharmacology ; Gelatin/pharmacology ; Exosomes/metabolism ; Wound Healing ; Diabetes Complications/metabolism ; Human Umbilical Vein Endothelial Cells ; Platelet-Rich Plasma/metabolism ; Diabetes Mellitus/metabolism
Chemical Substances	Hydrogels ; Gelatin (9000-70-8)
Language	English
Publishing date	2023-09-28
Publishing country	Netherlands
Document type	Journal Article
ISSN	2772-9508
ISSN (online)	2772-9508
DOI	10.1016/j.bioadv.2023.213640
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Article ; Online: Microneurosurgery and subsequent gamma knife radiosurgery for functioning pituitary macroadenomas or giant adenomas: One institution's experience.

Fu, Peng / He, Yun-Song / Cen, Yong-Cun / Huang, Qin / Guo, Ke-Tai / Zhao, Hong-Yang / Xiang, Wei

Clinical neurology and neurosurgery

2016 Volume 145, Page(s) 8–13

Abstract: Objective: Functioning pituitary macroadenoma and giant adenoma have large growth volumes and endocrinological abnormalities, requiring proper medical intervention. In this retrospective study, microneurosurgery and subsequent gamma knife radiosurgery ( ... ...

Abstract	Objective: Functioning pituitary macroadenoma and giant adenoma have large growth volumes and endocrinological abnormalities, requiring proper medical intervention. In this retrospective study, microneurosurgery and subsequent gamma knife radiosurgery (GKRS) is assessed for efficacy and safety for the treatment of functioning pituitary macroadenoma and giant adenoma. Methods: Between January 2007 and December 2011, 59 patients with functioning pituitary macroadenoma (n=38) or giant adenoma (n=21) received microneurosurgical resection, and after three months, GKRS with average maximum radiation dose ∼42Gy (range 30-66.7Gy). The median follow-up time was 54.3 months (range 36-85 months). Results: The combined treatment controlled tumor growth in 81.4% (48/59) of patients, and improved the endocrinological status in 64.4% (38/59). Complications included hypopituitarism and visual deterioration (22 and 7 patients, respectively). Large tumor size at presentation was a risk factor for tumor recurrence, but not age, gender, invasion, radiosurgical dose, pituitary hormone status or follow-up period. Better outcomes were achieved by patients with macroadenoma than giant adenoma. Conclusions: Combined microneurosurgery and GKRS are safe and effective for functioning pituitary macroadenomas or giant adenomas. Tumor control and endocrinological improvement were satisfactory, with minimal complications.
MeSH term(s)	Adenoma/radiotherapy ; Adenoma/surgery ; Adolescent ; Adult ; Aged ; Female ; Follow-Up Studies ; Humans ; Male ; Microsurgery/adverse effects ; Microsurgery/methods ; Middle Aged ; Neurosurgical Procedures/adverse effects ; Neurosurgical Procedures/methods ; Pituitary Neoplasms/radiotherapy ; Pituitary Neoplasms/surgery ; Radiosurgery/adverse effects ; Radiosurgery/methods ; Treatment Outcome ; Young Adult
Language	English
Publishing date	2016-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	193107-6
ISSN	1872-6968 ; 0303-8467
ISSN (online)	1872-6968
ISSN	0303-8467
DOI	10.1016/j.clineuro.2016.03.021
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Article ; Online: Fluoride Induces Neuroinflammation and Alters Wnt Signaling Pathway in BV2 Microglial Cells.

Chen, Rui / Zhao, Lian-Dong / Liu, Hong / Li, Hui-Hua / Ren, Chao / Zhang, Peng / Guo, Ke-Tai / Zhang, Hong-Xi / Geng, De-Qin / Zhang, Cai-Yi

Inflammation

2017 Volume 40, Issue 4, Page(s) 1123–1130

Abstract: Fluoride is a common element in nature and our daily life, and excessive intake of this element can cause fluorosis and irreversible brain damage. The toxic effects of fluoride on the central nervous system may be attributed to the release of ... ...

Abstract	Fluoride is a common element in nature and our daily life, and excessive intake of this element can cause fluorosis and irreversible brain damage. The toxic effects of fluoride on the central nervous system may be attributed to the release of inflammatory cytokines and ROS. GSK3β is a key protein that modulates NF-κB activity and inflammatory cytokine levels and plays an important role in the Wnt signaling pathway. In this study, we found that fluoride altered the inflammatory status and oxidative stress by inhibiting Wnt signaling pathway activity. This study thus provides a valid basis for the fluorine-induced neuroinflammation injury theory.
Language	English
Publishing date	2017-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	434408-x
ISSN	1573-2576 ; 0360-3997
ISSN (online)	1573-2576
ISSN	0360-3997
DOI	10.1007/s10753-017-0556-y
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Article ; Online: CELL-SELEX

Hans P. Wendel / Angela Paul / Gerhard Ziemer / Ke-Tai Guo

International Journal of Molecular Sciences, Vol 9, Iss 4, Pp 668-

Novel Perspectives of Aptamer-Based Therapeutics

2008 Volume 678

Abstract: Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) ... ...

Abstract	Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) targeting whole living cells has raised great expectations for cancer biology, -therapy and regenerative medicine. Combining nanobiotechnology with aptamers, this technology opens the way to more sophisticated applications in molecular diagnosis. This paper gives a review of recent developments in SELEX technologies and new applications of aptamers.
Keywords	cell-SELEX ; aptamer ; nanotechnology ; Chemistry ; QD1-999 ; Science ; Q ; DOAJ:Chemistry (General) ; DOAJ:Chemistry
Language	English
Publishing date	2008-04-01T00:00:00Z
Publisher	Molecular Diversity Preservation International
Document type	Article ; Online
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Article ; Online: EGb-761 Attenuates the Anti-proliferative Activity of Fluoride via DDK1 in PC-12 Cells.

Zhang, Cai-Yi / Chen, Rui / Wang, Fen / Ren, Chao / Zhang, Peng / Li, Qian / Li, Hui-Hua / Guo, Ke-Tai / Geng, De-Qin / Liu, Chun-Feng

Neurochemical research

2017 Volume 42, Issue 2, Page(s) 606–614

Abstract: EGb-761 is commonly used as a treatment for ischemic brain injury, neurodegenerative diseases and some types of tumors (Christen and Maixent, in Cell Mol Biol 48(6):601-611, 2002). However, it is unclear whether EGb-761 affects the proliferation of cells ...

Abstract	EGb-761 is commonly used as a treatment for ischemic brain injury, neurodegenerative diseases and some types of tumors (Christen and Maixent, in Cell Mol Biol 48(6):601-611, 2002). However, it is unclear whether EGb-761 affects the proliferation of cells exposed to fluoride. In this study, the proliferation and apoptosis of PC-12 cells exposed to fluoride were investigated and EGb-761 was used to protect PC-12 cells against the effects of fluoride. We found that the canonical Wnt signaling pathway was involved in the anti-proliferation of PC-12 cells exposed to fluoride. Furthermore, the results also showed that EGb-761 could attenuate the anti-proliferative activity of fluoride via DDK1 in PC-12 cells. This study may provide a new method for protecting against the inhibition of cell proliferation induced by fluoride.
MeSH term(s)	Animals ; Cell Proliferation/drug effects ; Cell Proliferation/physiology ; Dose-Response Relationship, Drug ; Exodeoxyribonucleases/biosynthesis ; PC12 Cells ; Plant Extracts/pharmacology ; Rats ; Sodium Fluoride/toxicity
Chemical Substances	Plant Extracts ; Ginkgo biloba extract (19FUJ2C58T) ; Sodium Fluoride (8ZYQ1474W7) ; Exodeoxyribonucleases (EC 3.1.-) ; MGME1 protein, human (EC 3.1.-)
Language	English
Publishing date	2017-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	199335-5
ISSN	1573-6903 ; 0364-3190
ISSN (online)	1573-6903
ISSN	0364-3190
DOI	10.1007/s11064-016-2115-6
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Article ; Online: CELL-SELEX: Novel perspectives of aptamer-based therapeutics.

Guo, Ke-Tai / Ziemer, Gerhard / Paul, Angela / Wendel, Hans P

International journal of molecular sciences

2008 Volume 9, Issue 4, Page(s) 668–678

Abstract	Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) targeting whole living cells has raised great expectations for cancer biology, -therapy and regenerative medicine. Combining nanobiotechnology with aptamers, this technology opens the way to more sophisticated applications in molecular diagnosis. This paper gives a review of recent developments in SELEX technologies and new applications of aptamers.
Language	English
Publishing date	2008-04-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms9040668
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