Article: Study on DNA extraction methods for AFLP analysis in chieh-qua
Molecular Plant Breeding
Mar.2009 , Issue (2)
Abstract: 本研究以CTAB法为基础,采用以下5种处理提取节瓜叶片基因组DNA:A、PVPP(3%)+抗坏血酸(100mmol/L);B、β-巯基乙醇(3%)+抗坏血酸(100mmol/L);C、PVPP(3%)+半胱氨酸(5%);D、PVPP(3%)+β-巯基乙醇(3%);E、抗坏血酸(100mmol/L)+半胱氨酸(5%)。实验结果表明:在5种处理中,C处理提取的DNA质量最高,其溶液呈无色透明状,经核酸蛋白仪测定其OD260/280为1.8~2.0;经0.8%琼脂糖凝胶电泳检测,节瓜叶片基因组DNA带型清晰、 ... ...
Abstract | 本研究以CTAB法为基础,采用以下5种处理提取节瓜叶片基因组DNA:A、PVPP(3%)+抗坏血酸(100mmol/L);B、β-巯基乙醇(3%)+抗坏血酸(100mmol/L);C、PVPP(3%)+半胱氨酸(5%);D、PVPP(3%)+β-巯基乙醇(3%);E、抗坏血酸(100mmol/L)+半胱氨酸(5%)。实验结果表明:在5种处理中,C处理提取的DNA质量最高,其溶液呈无色透明状,经核酸蛋白仪测定其OD260/280为1.8~2.0;经0.8%琼脂糖凝胶电泳检测,节瓜叶片基因组DNA带型清晰、完整、无降解,蛋白、多糖、酚类及RNA等去除比较彻底;经AFLP分析,酶切彻底,PCR扩增后的带型清晰稳定,重复性好,说明C处理提取的节瓜基因组DNA完全满足AFLP分析的要求。[著者文摘] |
---|---|
Keywords | BENIN ; DNA ; EXTRACTION ; BENIN ; ADN ; EXTRACTION ; BENIN ; ADN ; EXTRACCION ; http://www.fao.org/aos/agrovoc#c_875 ; http://www.fao.org/aos/agrovoc#c_2347 ; http://www.fao.org/aos/agrovoc#c_36910 |
Language | zho |
Document type | Article |
ISSN | 1672-416X |
Database | AGRIS - International Information System for the Agricultural Sciences and Technology |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.