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  1. Article: CME-Sonografie 98: Thoraxsonografie.

    Bürge, Tobias / Tuma, Jan

    Praxis

    2021  Volume 110, Issue 8, Page(s) 453–459

    Abstract: CME Sonography 98: Chest ... ...

    Title translation CME Sonography 98: Chest Ultrasound.
    Abstract CME Sonography 98: Chest Ultrasound
    MeSH term(s) COVID-19 ; Humans ; Pandemics ; Pleural Effusion/diagnostic imaging ; Pneumothorax/diagnostic imaging ; SARS-CoV-2 ; Ultrasonography
    Language German
    Publishing date 2021-05-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 209026-0
    ISSN 1661-8165 ; 1661-8157 ; 0369-8394
    ISSN (online) 1661-8165
    ISSN 1661-8157 ; 0369-8394
    DOI 10.1024/1661-8157/a003680
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: CME-Sonografie 98/Antworten: Thoraxsonografie.

    Bürge, Tobias / Tuma, Jan

    Praxis

    2021  Volume 110, Issue 9, Page(s) 508–509

    Abstract: CME Sonography 98/Answers: Chest ... ...

    Title translation CME Sonography 98/Answers: Chest Ultrasound.
    Abstract CME Sonography 98/Answers: Chest Ultrasound
    MeSH term(s) COVID-19 ; Humans ; Pandemics ; Pleural Effusion/diagnostic imaging ; Pneumothorax/diagnostic imaging ; SARS-CoV-2 ; Ultrasonography
    Language German
    Publishing date 2021-07-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 209026-0
    ISSN 1661-8165 ; 1661-8157 ; 0369-8394
    ISSN (online) 1661-8165
    ISSN 1661-8157 ; 0369-8394
    DOI 10.1024/1661-8157/a003681
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection.

    Koban, Robert / Lam, Tobias / Schwarz, Franziska / Kloke, Lutz / Bürge, Silvio / Ellerbrok, Heinz / Neumann, Markus

    Viruses

    2020  Volume 12, Issue 11

    Abstract: Studies of virus-host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may ... ...

    Abstract Studies of virus-host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropism, cytopathogenicity, and genomic organisation and virus morphology. Infections were characterised on molecular and imaging level. The transparent matrix substance allowed easy optical monitoring of cells and infection even via live-cell microscopy. In conclusion, we established an enhanced, standardised, easy-to-handle bioprinted 3D-cell culture system. The infection models are suitable for sensitive monitoring and characterisation of virus-host interactions and replication of different viruses under physiologically relevant conditions. Individual cell culture models can further be combined to a multicellular array. This generates a potent diagnostic tool for propagation and characterisation of viruses from diagnostic samples.
    MeSH term(s) Animals ; Biomarkers ; Bioprinting/methods ; Cell Culture Techniques ; Cell Survival ; Chlorocebus aethiops ; Host-Pathogen Interactions ; Humans ; Molecular Imaging ; Spheroids, Cellular ; Vero Cells ; Virus Diseases/diagnosis ; Virus Diseases/virology
    Chemical Substances Biomarkers
    Language English
    Publishing date 2020-11-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12111298
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection

    Koban, Robert / Lam, Tobias / Schwarz, Franziska / Kloke, Lutz / Bürge, Silvio / Ellerbrok, Heinz / Neumann, Markus

    Viruses. 2020 Nov. 12, v. 12, no. 11

    2020  

    Abstract: Studies of virus–host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may ... ...

    Abstract Studies of virus–host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropism, cytopathogenicity, and genomic organisation and virus morphology. Infections were characterised on molecular and imaging level. The transparent matrix substance allowed easy optical monitoring of cells and infection even via live-cell microscopy. In conclusion, we established an enhanced, standardised, easy-to-handle bioprinted 3D-cell culture system. The infection models are suitable for sensitive monitoring and characterisation of virus–host interactions and replication of different viruses under physiologically relevant conditions. Individual cell culture models can further be combined to a multicellular array. This generates a potent diagnostic tool for propagation and characterisation of viruses from diagnostic samples.
    Keywords cell culture ; cell structures ; cytopathogenicity ; diagnostic techniques ; genomics ; host range ; host-pathogen relationships ; image analysis ; microscopy ; models ; monitoring ; tissue tropism ; viruses
    Language English
    Dates of publication 2020-1112
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12111298
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection

    Koban, Robert / Lam, Tobias / Schwarz, Franziska / Kloke, Lutz / Bürge, Silvio / Ellerbrok, Heinz / Neumann, Markus

    2020  

    Abstract: Studies of virus–host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may ... ...

    Abstract Studies of virus–host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropism, cytopathogenicity, and genomic organisation and virus morphology. Infections were characterised on molecular and imaging level. The transparent matrix substance allowed easy optical monitoring of cells and infection even via live-cell microscopy. In conclusion, we established an enhanced, standardised, easy-to-handle bioprinted 3D-cell culture system. The infection models are suitable for sensitive monitoring and characterisation of virus–host interactions and replication of different viruses under physiologically relevant conditions. Individual cell culture models can further be combined to a multicellular array. This generates a potent diagnostic tool for propagation and characterisation of viruses from diagnostic samples.

    Peer Reviewed
    Keywords 3D cell cultivation ; 3D bioprinting ; virus infection ; detection ; infection model ; 610 Medizin und Gesundheit ; ddc:610
    Language English
    Publishing date 2020-11-12
    Publisher Robert Koch-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Direct Comparison of 4 Very Early Rule-Out Strategies for Acute Myocardial Infarction Using High-Sensitivity Cardiac Troponin I.

    Boeddinghaus, Jasper / Nestelberger, Thomas / Twerenbold, Raphael / Wildi, Karin / Badertscher, Patrick / Cupa, Janosch / Bürge, Tobias / Mächler, Patrick / Corbière, Sydney / Grimm, Karin / Giménez, Maria Rubini / Puelacher, Christian / Shrestha, Samyut / Flores Widmer, Dayana / Fuhrmann, Jakob / Hillinger, Petra / Sabti, Zaid / Honegger, Ursina / Schaerli, Nicolas /
    Kozhuharov, Nikola / Rentsch, Katharina / Miró, Òscar / López, Beatriz / Martin-Sanchez, F Javier / Rodriguez-Adrada, Esther / Morawiec, Beata / Kawecki, Damian / Ganovská, Eva / Parenica, Jiri / Lohrmann, Jens / Kloos, Wanda / Buser, Andreas / Geigy, Nicolas / Keller, Dagmar I / Osswald, Stefan / Reichlin, Tobias / Mueller, Christian

    Circulation

    2017  Volume 135, Issue 17, Page(s) 1597–1611

    Abstract: Background: Four strategies for very early rule-out of acute myocardial infarction using high-sensitivity cardiac troponin I (hs-cTnI) have been identified. It remains unclear which strategy is most attractive for clinical application.: Methods: We ... ...

    Abstract Background: Four strategies for very early rule-out of acute myocardial infarction using high-sensitivity cardiac troponin I (hs-cTnI) have been identified. It remains unclear which strategy is most attractive for clinical application.
    Methods: We prospectively enrolled unselected patients presenting to the emergency department with symptoms suggestive of acute myocardial infarction. The final diagnosis was adjudicated by 2 independent cardiologists. Hs-cTnI levels were measured at presentation and after 1 hour in a blinded fashion. We directly compared all 4 hs-cTnI-based rule-out strategies: limit of detection (LOD, hs-cTnI<2 ng/L), single cutoff (hs-cTnI<5 ng/L), 1-hour algorithm (hs-cTnI<5 ng/L and 1-hour change<2 ng/L), and the 0/1-hour algorithm recommended in the European Society of Cardiology guideline combining LOD and 1-hour algorithm.
    Results: Among 2828 enrolled patients, acute myocardial infarction was the final diagnosis in 451 (16%) patients. The LOD approach ruled out 453 patients (16%) with a sensitivity of 100% (95% confidence interval [CI], 99.2%-100%), the single cutoff 1516 patients (54%) with a sensitivity of 97.1% (95% CI, 95.1%-98.3%), the 1-hour algorithm 1459 patients (52%) with a sensitivity of 98.4% (95% CI, 96.8%-99.2%), and the 0/1-hour algorithm 1463 patients (52%) with a sensitivity of 98.4% (95% CI, 96.8%-99.2%). Predefined subgroup analysis in early presenters (≤2 hours) revealed significantly lower sensitivity (94.2%, interaction
    Conclusions: All 4 rule-out strategies balance effectiveness and safety equally well. The single cutoff should not be applied in early presenters, whereas the 3 other strategies seem to perform well in this challenging subgroup.
    Clinical trial registration: URL: http://www.clinicaltrials.gov. Unique identifier: NCT00470587.
    MeSH term(s) Acute Coronary Syndrome/blood ; Acute Coronary Syndrome/diagnosis ; Acute Coronary Syndrome/mortality ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Algorithms ; Biomarkers/blood ; Decision Support Techniques ; Electrocardiography ; Europe ; Female ; Health Status ; Humans ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Myocardial Infarction/blood ; Myocardial Infarction/diagnosis ; Myocardial Infarction/mortality ; Predictive Value of Tests ; Prognosis ; Prospective Studies ; Risk Assessment ; Risk Factors ; Sex Factors ; Time Factors ; Troponin I/blood ; Up-Regulation
    Chemical Substances Biomarkers ; Troponin I
    Language English
    Publishing date 2017-03-10
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Multicenter Study
    ZDB-ID 80099-5
    ISSN 1524-4539 ; 0009-7322 ; 0069-4193 ; 0065-8499
    ISSN (online) 1524-4539
    ISSN 0009-7322 ; 0069-4193 ; 0065-8499
    DOI 10.1161/CIRCULATIONAHA.116.025661
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Early diagnosis of acute myocardial infarction in patients with mild elevations of cardiac troponin.

    Boeddinghaus, Jasper / Reichlin, Tobias / Nestelberger, Thomas / Twerenbold, Raphael / Meili, Yvette / Wildi, Karin / Hillinger, Petra / Giménez, Maria Rubini / Cupa, Janosch / Schumacher, Lukas / Schubera, Marie / Badertscher, Patrick / Corbière, Sydney / Grimm, Karin / Puelacher, Christian / Sabti, Zaid / Widmer, Dayana Flores / Schaerli, Nicolas / Kozhuharov, Nikola /
    Shrestha, Samyut / Bürge, Tobias / Mächler, Patrick / Büchi, Michael / Rentsch, Katharina / Miró, Òscar / López, Beatriz / Martin-Sanchez, F Javier / Rodriguez-Adrada, Esther / Morawiec, Beata / Kawecki, Damian / Ganovská, Eva / Parenica, Jiri / Lohrmann, Jens / Buser, Andreas / Keller, Dagmar I / Osswald, Stefan / Mueller, Christian

    Clinical research in cardiology : official journal of the German Cardiac Society

    2017  Volume 106, Issue 6, Page(s) 457–467

    Abstract: Background: The early diagnosis of acute myocardial infarction (AMI) in patients with mild elevations of high-sensitivity cardiac troponin (hs-cTn) is a challenge. It is unclear whether copeptin, a marker of endogenous stress, or 1h-hs-cTn changes are ... ...

    Abstract Background: The early diagnosis of acute myocardial infarction (AMI) in patients with mild elevations of high-sensitivity cardiac troponin (hs-cTn) is a challenge. It is unclear whether copeptin, a marker of endogenous stress, or 1h-hs-cTn changes are better suited to address this important unmet clinical need.
    Methods: We prospectively enrolled patients presenting with symptoms suggestive of AMI to the emergency department (ED). Two independent cardiologists adjudicated the final diagnosis. Mild hs-cTn elevations were defined as 26.2 ng/L (99th percentile) to 75 ng/L for hs-cTnI, and 14 ng/L (99th percentile) to 50 ng/L (biological-equivalent to 75 ng/L for hs-cTnI) for hs-cTnT.
    Results: Among 1356 patients, 80 (6%) had mild hs-cTnI elevations at presentation. Within this group, AMI was the final diagnosis in 39 patients (49%). The diagnostic accuracy for the diagnosis of AMI as quantified by the area under the receiver operating characteristic curve (AUC) was 0.51 (95% CI 0.39-0.64) for hs-cTnI at presentation, 0.58 (95% CI 0.45-0.71) for copeptin at presentation, and 0.78 (95% CI 0.68-0.88) for 1h-hs-cTnI changes, which was significantly higher as compared to copeptin (p = 0.02) or hs-cTnI alone (p < 0.001). The additional use of 1h-hs-cTnI changes, but not of copeptin, improved diagnostic accuracy of hs-cTnI at presentation (AUC 0.80, 95% CI 0.70-0.90; p = 0.002 for comparison). Similar findings regarding copeptin and 1h-hs-cTnT/I changes were obtained for mild hs-cTnT elevations.
    Conclusions: About 6-22% of patients presenting with suggestive AMI to the ED have mild hs-cTnT/I elevations at presentation. In contrast to copeptin, the addition of 1h-hs-cTn changes substantially improves the early diagnosis of AMI.
    Language English
    Publishing date 2017-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2213295-8
    ISSN 1861-0692 ; 1861-0684
    ISSN (online) 1861-0692
    ISSN 1861-0684
    DOI 10.1007/s00392-016-1075-9
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  8. Article: Comparative genomics reveals insights into avian genome evolution and adaptation

    Zhang, Guojie / Agostinho Antunes / Alonzo Alfaro-Núñez / Amhed Missael Vargas Velazquez / Anders Ödeen / Andreas Pfenning / Andrew Dixon / Angela M. Ribeiro / Bo Li / Bo Wang / Cai Li / Carsten Rahbek / Chen Ye / Chul Lee / Claudio V. Mello / Daniela Almeida / Darren Griffin / David A. Ray / David Haussler /
    David Lambert / David P. Froman / David P. Mindell / David W. Burt / Denis M. Larkin / Douglas Rhoads / Edward C. Holmes / Edward L. Braun / Elizabeth Derryberry / Emanuel Maldonado / Eric Lyons / Erich D. Jarvis / Erina Hara / Eske Willerslev / Fang Zhang / Federico G. Hoffmann / Fiona McCarthy / Frederick H. Sheldon / Frederique Pitel / Gancho Slavov / Ganeshkumar Ganapathy / Gary R. Graves / Guangbiao Wang / Hailin Pan / Hao Yu / Haofu Hu / Heebal Kim / Huanming Yang / Hui Li / Hyeon Jeong Kim / Imran Khan / Jacqueline Smith / Jason T. Howard / Jay F. Storz / Jian Wang / Jiang Hu / Jie Cui / Jie Wang / Jin Xiao / Jingjing Wang / Jinmin Lian / Jitendra Narayan / João Paulo Machado / Joana Pereira / John Gatesy / Jon Fjeldså / José Alfredo Samaniego / Juan C. Opazo / Julia Smith / Jun Wang / Kartik Sunagar / Kyu-Won Kim / Lijun Jin / Long Zhou / Ludovic Orlando / Luohao Xu / M. Lisandra Zepeda / M. Thomas P. Gilbert / Mads F. Bertelsen / María Avila-Arcos / Maria Gloria Dominguez-Bello / Mark S. Springer / Marta Farré / Matthew J. Greenwold / Michael Bunce / Michael D. Martin / Michael N Romanov / Michael W. Bruford / Mikkel Schubert / Miriam V. Rivas / Na An / Ning Li / Oliver A. Ryder / Olle Håstad / Osceola Whitney / Paul P. Gardner / Paula F. Campos / Pei Zhang / Per Alström / Peter F. Stadler / Peter Houde / Peter V. Lovell / Ping Wen / Qi Zhou / Qiaolin Xie / Qiumei Zheng / Qiye Li / Richard E. Green / Richard K Wilson / Robb T. Brumfield / Robert W. Meredith / Roger H. Sawyer / Ross Barnett / Rui Borges / Rute R. da Fonseca / Sarah W. Burge / Scott V. Edwards / Seoae Cho / Shaoguang Liang / Shengbin Li / Shiping Liu / Siby Philip / Stephen J. O’Brien / Tammy E. Steeves / Tobias Mourier / Warren E. Johnson / Wei Yang / Wesley Warren / Xiangjiang Zhan / Yang Liu / Yingqi Xiong / Yinhua Huang / Yong Zhang / Yongli Zeng / Yu Fan / Zengli Yan / Zhikai Yang / Zhiyong Huang / Zijun Xiong / Zongji Wang

    Science. 2014 Dec. 12, v. 346, no. 6215

    2014  

    Abstract: Birds are the most species-rich class of tetrapod vertebrates and have wide relevance across many research fields. We explored bird macroevolution using full genomes from 48 avian species representing all major extant clades. The avian genome is ... ...

    Abstract Birds are the most species-rich class of tetrapod vertebrates and have wide relevance across many research fields. We explored bird macroevolution using full genomes from 48 avian species representing all major extant clades. The avian genome is principally characterized by its constrained size, which predominantly arose because of lineage-specific erosion of repetitive elements, large segmental deletions, and gene loss. Avian genomes furthermore show a remarkably high degree of evolutionary stasis at the levels of nucleotide sequence, gene synteny, and chromosomal structure. Despite this pattern of conservation, we detected many non-neutral evolutionary changes in protein-coding genes and noncoding regions. These analyses reveal that pan-avian genomic diversity covaries with adaptations to different lifestyles and convergent evolution of traits.
    Keywords birds ; convergent evolution ; gene deletion ; genes ; genetic variation ; genomics ; lifestyle ; nucleotide sequences
    Language English
    Dates of publication 2014-1212
    Size p. 1311-1320.
    Publishing place American Association for the Advancement of Science
    Document type Article
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.1251385
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