Result 1 - 8 of total 8
Eur. J. Risk Regul.
| Keywords | covid19 |
|---|---|
| Publisher | WHO |
| Document type | Article |
| Note | WHO #Covidence: #31161 |
| Database | COVID19 |
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European Journal of Risk Regulation
2020 Volume 11, Issue 2, Page(s) 390–395
| Keywords | Safety Research ; covid19 |
|---|---|
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publishing country | uk |
| Document type | Article ; Online |
| ZDB-ID | 2600871-3 |
| ISSN | 2190-8249 ; 1867-299X |
| ISSN (online) | 2190-8249 |
| ISSN | 1867-299X |
| DOI | 10.1017/err.2020.24 |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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Methods in molecular biology (Clifton, N.J.)
2021 Volume 2348, Page(s) 71–90
Abstract: Mammalian genomes are pervasively transcribed and a small fraction of RNAs produced codify for proteins. The importance of noncoding RNAs for the maintenance of cell functions is well known (e.g., rRNAs, tRNAs), but only recently it was first ... ...
| Abstract | Mammalian genomes are pervasively transcribed and a small fraction of RNAs produced codify for proteins. The importance of noncoding RNAs for the maintenance of cell functions is well known (e.g., rRNAs, tRNAs), but only recently it was first demonstrated the involvement of microRNAs (miRNAs) in posttranscriptional regulation and then the activity of long noncoding RNAs (lncRNAs) in the regulation of miRNAs, DNA structure and protein function. LncRNAs have an expression more cell specific than other RNAs and basing on their subcellular localization exert different functions. In this book chapter we consider different protocols to evaluate the expression of lncRNAs at the single cell level using genome-wide approaches. We considered the skeletal muscle as example because the most abundant tissue in mammals involved in the regulation of metabolism and body movement. We firstly described how to isolate the smallest complete contractile system responsible for muscle metabolic and contractile traits (myofibers). We considered how to separate long and short RNAs to allow the sequencing of the full-length transcript using the SMART technique for the retrotranscription. Because of myofibers are multinucleated cells and because of it is better to perform single cell sequencing on fresh tissues we described the single-nucleus sequencing that can be applied to frozen tissues. The chapter concludes with a description of bioinformatics approaches to evaluate differential expression from single-cell or single-nucleus RNA sequencing. |
|---|---|
| MeSH term(s) | Computational Biology/methods ; Gene Expression Regulation ; Gene Library ; MicroRNAs/genetics ; Muscle Fibers, Skeletal/metabolism ; Polyadenylation ; RNA Processing, Post-Transcriptional ; RNA, Long Noncoding/genetics ; RNA, Messenger/genetics ; Sequence Analysis, RNA/methods ; Single-Cell Analysis/methods |
| Chemical Substances | MicroRNAs ; RNA, Long Noncoding ; RNA, Messenger |
| Language | English |
| Publishing date | 2021-06-23 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-0716-1581-2_5 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Revista de derecho y genoma humano = Law and the human genome review
2015 , Issue 42, Page(s) 161–167
Abstract: On 18 December 2014, in International Stem Cell Corporation v. Comptroller General of Patents, Designs and Trade Marks (C-364/13), the Court of Justice of the European Union (CJEU) delivered an important decision regarding the scope of the exclusion from ...
| Abstract | On 18 December 2014, in International Stem Cell Corporation v. Comptroller General of Patents, Designs and Trade Marks (C-364/13), the Court of Justice of the European Union (CJEU) delivered an important decision regarding the scope of the exclusion from patentability on morality-related grounds under Article 6(2) of the EU Biotech Directive. The Court made an important distinction between embryonic stem cell technologies based on fertilised human ovum and those based on unfertilised human ovum stimulated by parthenogenesis. The CJEU held, in particular, that a human ovum: (i) who is unfertilized and (ii) whose division and further development has been stimulated by parthenogenesis, is not a human embryo under Article 6(2)(c) of the Biotech Directive, if it in itself has not the inherent capacity of developing into a human being, this matter to be ascertained by the national court in the light of current scientific knowledge. By doing so, the Court clarified its previous ruling in Brüstle (October 2011). |
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| MeSH term(s) | European Union ; Human Embryonic Stem Cells ; Humans ; Patents as Topic/legislation & jurisprudence |
| Language | English |
| Publishing date | 2015-01 |
| Publishing country | Spain |
| Document type | Journal Article ; Legal Cases |
| ISSN | 1134-7198 |
| ISSN | 1134-7198 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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International journal of molecular sciences
2020 Volume 21, Issue 1
Abstract: In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most ... ...
| Abstract | In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-coding RNAs (lncRNAs). In contrast to protein-coding RNAs, they show a more cell-specific expression. To understand the function of lncRNAs, it is fundamental to investigate in which cells they are preferentially expressed and to detect their subcellular localization. Recent improvements of techniques that localize single RNA molecules in tissues like single-cell RNA sequencing and fluorescence amplification methods have given a considerable boost in the knowledge of the lncRNA functions. In recent years, single-cell transcription variability was associated with non-coding RNA expression, revealing this class of RNAs as important transcripts in the cell lineage specification. The purpose of this review is to collect updated information about lncRNA classification and new findings on their function derived from single-cell analysis. We also retained useful for all researchers to describe the methods available for single-cell analysis and the databases collecting single-cell and lncRNA data. Tables are included to schematize, describe, and compare exposed concepts. |
|---|---|
| MeSH term(s) | Cell Lineage ; Databases, Genetic ; Gene Expression Regulation ; Humans ; MicroRNAs/antagonists & inhibitors ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Neoplasms/genetics ; Neoplasms/pathology ; RNA Splicing ; RNA, Long Noncoding/antagonists & inhibitors ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; RNA, Ribosomal/genetics ; RNA, Ribosomal/metabolism ; Single-Cell Analysis |
| Chemical Substances | MicroRNAs ; RNA, Long Noncoding ; RNA, Ribosomal |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-01-01 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Review |
| ZDB-ID | 2019364-6 |
| ISSN | 1422-0067 ; 1422-0067 ; 1661-6596 |
| ISSN (online) | 1422-0067 |
| ISSN | 1422-0067 ; 1661-6596 |
| DOI | 10.3390/ijms21010302 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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International Journal of Molecular Sciences, Vol 21, Iss 1, p
A Journey into the World of Long Non-Coding RNAs
2020 Volume 302
Abstract: In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most ... ...
| Abstract | In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-coding RNAs (lncRNAs). In contrast to protein-coding RNAs, they show a more cell-specific expression. To understand the function of lncRNAs, it is fundamental to investigate in which cells they are preferentially expressed and to detect their subcellular localization. Recent improvements of techniques that localize single RNA molecules in tissues like single-cell RNA sequencing and fluorescence amplification methods have given a considerable boost in the knowledge of the lncRNA functions. In recent years, single-cell transcription variability was associated with non-coding RNA expression, revealing this class of RNAs as important transcripts in the cell lineage specification. The purpose of this review is to collect updated information about lncRNA classification and new findings on their function derived from single-cell analysis. We also retained useful for all researchers to describe the methods available for single-cell analysis and the databases collecting single-cell and lncRNA data. Tables are included to schematize, describe, and compare exposed concepts. |
|---|---|
| Keywords | single-cell ; single-cell sequencing ; single-cell expression ; non-coding rnas ; long non-coding rnas ; lncrnas ; lncrna database ; single-cell database ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999 |
| Subject code | 572 |
| Language | English |
| Publishing date | 2020-01-01T00:00:00Z |
| Publisher | MDPI AG |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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ESMO open
2020 Volume 5, Issue 6, Page(s) e000988
Abstract: Effective networking and mentoring are critical determinants of professional satisfaction and success in oncology. There are multiple benefits associated with established mentoring programs. However, these are scarce in Latin America (LATAM). The AAZPIRE ...
| Abstract | Effective networking and mentoring are critical determinants of professional satisfaction and success in oncology. There are multiple benefits associated with established mentoring programs. However, these are scarce in Latin America (LATAM). The AAZPIRE project meeting was held to encourage the discussion of mentorship strategies in our region, to create new learning frameworks, and improve cancer care. A group of 30 young oncologists and investigators, together with seven members of LACOG and CLICaP experts of 8 LATAM countries, were reunited to share views and define opportunities, barriers, and possible solutions to implement mentorship programs in LATAM. For each of the mentioned topics, key points were obtained by consensus, and a literature review was conducted to support group conclusions. This article analyses mentoring in LATAM countries and its role on promoting leadership. It will address conceptual frameworks, limitations, and opportunities from the perspectives of both mentor and mentee. The creation of regional and international group stimulation programs and joint projects that impact health policies are attractive, starting points to implement mentorship scenarios. |
|---|---|
| MeSH term(s) | Humans ; Latin America ; Mentoring ; Mentors ; Neoplasms/therapy ; Research Personnel |
| Language | English |
| Publishing date | 2020-11-21 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Review |
| ISSN | 2059-7029 |
| ISSN (online) | 2059-7029 |
| DOI | 10.1136/esmoopen-2020-000988 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
2021 Volume 142, Page(s) 112000
Abstract: The main goal of this study is to evaluate the efficacy of the paclitaxel (PTX) drug formulated with a liposomal nanosystem (L-PTX) in a peritoneal carcinomatosis derived from ovarian cancer. In vitro cell viability studies with the human ovarian cancer ... ...
| Abstract | The main goal of this study is to evaluate the efficacy of the paclitaxel (PTX) drug formulated with a liposomal nanosystem (L-PTX) in a peritoneal carcinomatosis derived from ovarian cancer. In vitro cell viability studies with the human ovarian cancer line A2780 showed a 50% decrease in the inhibitory concentration for L-PTX compared to free PTX. A2780 cells treated with the L-PTX formulation demonstrated a reduced capacity to form colonies in comparison to those treated with PTX. Cell death following L-PTX administration hinted at apoptosis, with most cells undergoing initial apoptosis. A2780 cells exhibited an inhibitory migration profile when analyzed by Wound Healing and real-time cell analysis (xCELLigence) methods after L-PTX administration. This inhibition was related to decreased expression of the zinc finger E-box-binding homeobox 1 (ZEB1) and transforming growth factor 2 (TGF-β2) genes. In vivoL-PTX administration strongly inhibited tumor cell proliferation in ovarian peritoneal carcinomatosis derived from ovarian cancer, indicating higher antitumor activity than PTX. L-PTX formulation did not show toxicity in the mice model. This study demonstrated that liposomal paclitaxel formulations are less toxic to normal tissues than free paclitaxel and are more effective in inhibiting tumor cell proliferation/migration and inducing ZEB1/TGF-β2 gene expression. |
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| MeSH term(s) | Animals ; Antineoplastic Agents, Phytogenic/administration & dosage ; Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Carcinoma, Ovarian Epithelial/drug therapy ; Carcinoma, Ovarian Epithelial/genetics ; Carcinoma, Ovarian Epithelial/pathology ; Cell Death/drug effects ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Liposomes ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/pathology ; Paclitaxel/administration & dosage ; Paclitaxel/pharmacology ; Peritoneal Neoplasms/drug therapy |
| Chemical Substances | Antineoplastic Agents, Phytogenic ; Liposomes ; Paclitaxel (P88XT4IS4D) |
| Language | English |
| Publishing date | 2021-08-20 |
| Publishing country | France |
| Document type | Comparative Study ; Journal Article |
| ZDB-ID | 392415-4 |
| ISSN | 1950-6007 ; 0753-3322 ; 0300-0893 |
| ISSN (online) | 1950-6007 |
| ISSN | 0753-3322 ; 0300-0893 |
| DOI | 10.1016/j.biopha.2021.112000 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Ud II Zs.198: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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