Article ; Online: Mycobacterial membrane protein Large 3-like-family proteins in bacteria, protozoa, fungi, plants, and animals: A bioinformatics and structural investigation.
2021 Volume 90, Issue 3, Page(s) 776–790
Abstract: Lipid transporters play an important role in most if not all organisms, ranging from bacteria to humans. For example, in Mycobacterium tuberculosis, the trehalose monomycolate transporter MmpL3 is involved in cell wall biosynthesis, while in humans, ... ...
| Abstract | Lipid transporters play an important role in most if not all organisms, ranging from bacteria to humans. For example, in Mycobacterium tuberculosis, the trehalose monomycolate transporter MmpL3 is involved in cell wall biosynthesis, while in humans, cholesterol transporters are involved in normal cell function as well as in disease. Here, using structural and bioinformatics information, we propose that there are proteins that also contain "MmpL3-like" (MMPL) transmembrane (TM) domains in many protozoa, including Trypanosoma cruzi, as well as in the bacterium Staphylococcus aureus, where the fatty acid transporter FarE has the same set of "active-site" residues as those found in the mycobacterial MmpL3s, and in T. cruzi. We also show that there are strong sequence and predicted structural similarities between the TM proton-translocation domain seen in the X-ray structures of mycobacterial MmpL3s and several human as well as fungal lipid transporters, leading to the proposal that there are similar proteins in apicomplexan parasites, and in plants. The animal, fungal, apicomplexan, and plant proteins have larger extra-membrane domains than are found in the bacterial MmpL3, but they have a similar TM domain architecture, with the introduction of a (catalytically essential) Phe > His residue change, and a Ser/Thr H-bond network, involved in H |
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| MeSH term(s) | Amino Acid Sequence ; Bacterial Proteins/chemistry ; Biological Transport ; Catalytic Domain ; Cholesterol/chemistry ; Cord Factors/chemistry ; Fungi ; Membrane Transport Proteins/chemistry ; Models, Molecular ; Mycobacterium tuberculosis/metabolism ; Protein Binding ; Protein Conformation ; Protein Domains ; Staphylococcus aureus ; Structure-Activity Relationship ; Trypanosoma cruzi |
| Chemical Substances | Bacterial Proteins ; Cord Factors ; Membrane Transport Proteins ; trehalose monomycolate ; Cholesterol (97C5T2UQ7J) |
| Language | English |
| Publishing date | 2021-11-12 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 806683-8 |
| ISSN | 1097-0134 ; 0887-3585 |
| ISSN (online) | 1097-0134 |
| ISSN | 0887-3585 |
| DOI | 10.1002/prot.26273 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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