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  1. Article ; Online: Listening to School Nurses' Voices: A Mixed Methods Study on the Continued Impact of COVID-19 on School Nursing Practice.

    Hughes, M Laurette / White, Laura / O'Brien, Mary Jane / Aubin, Judy / Bradford, Carol

    The Journal of school nursing : the official publication of the National Association of School Nurses

    2024  , Page(s) 10598405241237726

    Abstract: School closures in March 2020 due to the COVID-19 pandemic precipitated losses of critical student resources as physical, mental, emotional, and social needs escalated. Identifying the challenges, strategies, and changes in school nurse (SN) practice in ... ...

    Abstract School closures in March 2020 due to the COVID-19 pandemic precipitated losses of critical student resources as physical, mental, emotional, and social needs escalated. Identifying the challenges, strategies, and changes in school nurse (SN) practice in Massachusetts during this pandemic is fundamental to understanding how to manage future anticipated pandemics while protecting children, communities, and SNs. The purpose of this mixed-methods descriptive study in the second year of the global pandemic was to (a) listen to SN voices through a novel online survey including the prompts of challenges, strategies, and practice changes and (b) describe the SN experience of COVID-19 response in Massachusetts schools, including identification of intent to leave school nursing. Responses were analyzed using descriptive qualitative analysis (
    Language English
    Publishing date 2024-04-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1217746-5
    ISSN 1546-8364 ; 1059-8405 ; 0048-945X
    ISSN (online) 1546-8364
    ISSN 1059-8405 ; 0048-945X
    DOI 10.1177/10598405241237726
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4182: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  2. Article ; Online: True volumetric counting of CD34+ cells using flow cytometry.

    Rico, Laura G / Bardina, Jorge / Salvia, Roser / Ward, Michael D / Bradford, Jolene A / Petriz, Jordi

    Journal of immunological methods

    2024  Volume 527, Page(s) 113649

    Abstract: While the single-platform flow cytometric CD34+ cell counting method is the preferred choice to predict the yield of mobilized peripheral blood stem cells, most flow cytometers lack the ability of hematology counter analyzers to perform volumetric ... ...

    Abstract While the single-platform flow cytometric CD34+ cell counting method is the preferred choice to predict the yield of mobilized peripheral blood stem cells, most flow cytometers lack the ability of hematology counter analyzers to perform volumetric counting. However, one of the problems using reference microbeads is the vanishing counting bead phenomenon. This phenomenon results in a drop in microbeads concentration and reduces the total and relative number of beads in calibration procedures. In the last years, flow cytometers including a volumetric system to quantify cells have been developed and may represent a promising alternative to enumerate CD34+ cells avoiding the use of beads. In this study we have used a direct true volumetric counting of CD34+ cells under continuous flow pump to overcome potential drawbacks with impact in rare cell analysis. To confirm this hypothesis, we have compared the results of CD34+ cell enumeration using non-volumetric vs. volumetric systems with FC500 (Beckman Coulter) and Attune NxT (ThermoFisher) flow cytometers, respectively, in mobilized peripheral blood samples. No statistically significant differences were observed between measurements of CD34+ cells using beads, when the FC500 and Attune NxT absolute counting values were compared, or when CD34+ counts were compared on the Attune NxT, either using or not using beads. Linear regressions to study the relationship between volumetric and non-volumetric CD34+ counts confirmed the accuracy of each method. Bland-Altman test showed agreement between both methods. Our data showed that CD34+ cell enumeration using a volumetric system is comparable with current counting systems. This method represents an alternative with the advantage of the simplification of sample preparation and the reduction of the analysis subjectivity.
    MeSH term(s) Flow Cytometry/methods ; Cell Count ; Linear Models ; Antigens, CD34 ; Microspheres
    Chemical Substances Antigens, CD34
    Language English
    Publishing date 2024-02-21
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2024.113649
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    Zs.A 795: Show issues Location:
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  3. Article ; Online: Thoracoabdominal asynchrony in a virtual preterm infant: computational modeling and analysis.

    Foster, Richard R / Smith, Bradford / Ellwein Fix, Laura

    American journal of physiology. Lung cellular and molecular physiology

    2023  Volume 325, Issue 2, Page(s) L190–L205

    Abstract: Thoracoabdominal asynchrony (TAA), the asynchronous volume changes between the rib cage and abdomen during breathing, is associated with respiratory distress, progressive lung volume loss, and chronic lung disease in the newborn infant. Preterm infants ... ...

    Abstract Thoracoabdominal asynchrony (TAA), the asynchronous volume changes between the rib cage and abdomen during breathing, is associated with respiratory distress, progressive lung volume loss, and chronic lung disease in the newborn infant. Preterm infants are prone to TAA risk factors such as weak intercostal muscles, surfactant deficiency, and a flaccid chest wall. The causes of TAA in this fragile population are not fully understood and, to date, the assessment of TAA has not included a mechanistic modeling framework to explore the role these risk factors play in breathing dynamics and how TAA can be resolved. We present a dynamic compartmental model of pulmonary mechanics that simulates TAA in the preterm infant under various adverse clinical conditions, including high chest wall compliance, applied inspiratory resistive loads, bronchopulmonary dysplasia, anesthesia-induced intercostal muscle deactivation, weakened costal diaphragm, impaired lung compliance, and upper airway obstruction. Sensitivity analyses performed to screen and rank model parameter influence on model TAA and respiratory volume outputs show that risk factors are additive so that maximal TAA occurs in a virtual preterm infant with multiple adverse conditions, and addressing risk factors individually causes incremental changes in TAA. An abruptly obstructed upper airway caused immediate nearly paradoxical breathing and tidal volume reduction despite greater effort. In most simulations, increased TAA occurred together with decreased tidal volume. Simulated indices of TAA are consistent with published experimental studies and clinically observed pathophysiology, motivating further investigation into the use of computational modeling for assessing and managing TAA.
    MeSH term(s) Infant ; Humans ; Infant, Newborn ; Infant, Premature/physiology ; Respiratory Mechanics/physiology ; Thorax/physiology ; Bronchopulmonary Dysplasia ; Computer Simulation
    Language English
    Publishing date 2023-06-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1013184-x
    ISSN 1522-1504 ; 1040-0605
    ISSN (online) 1522-1504
    ISSN 1040-0605
    DOI 10.1152/ajplung.00123.2022
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    Uc I Zs.89: Show issues Location:
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    Zs.A 2749: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article: Standard contractual clauses for cross-border transfers of health data after

    Bradford, Laura / Aboy, Mateo / Liddell, Kathleen

    Journal of law and the biosciences

    2021  Volume 8, Issue 1, Page(s) lsab007

    Abstract: Standard contractual clauses (SCCs) have long been considered the most accessible method to transfer personal data legally across borders. In July 2020, the Court of Justice of the European Union (CJEU) ... ...

    Abstract Standard contractual clauses (SCCs) have long been considered the most accessible method to transfer personal data legally across borders. In July 2020, the Court of Justice of the European Union (CJEU) in
    Language English
    Publishing date 2021-06-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2756090-9
    ISSN 2053-9711
    ISSN 2053-9711
    DOI 10.1093/jlb/lsab007
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  5. Article ; Online: Functional Flow Cytometry to Predict PD-L1 Conformational Changes.

    Salvia, Roser / Rico, Laura G / Ward, Michael D / Bradford, Jolene A / Petriz, Jordi

    Current protocols

    2023  Volume 3, Issue 12, Page(s) e944

    Abstract: The programmed cell death protein 1/programmed cell death protein ligand 1 (PD-1/PD-L1) axis is one of the most widely recognized targets for cancer immunotherapy. Importantly, PD-L1 conformational changes can hinder target binding when living cells are ... ...

    Abstract The programmed cell death protein 1/programmed cell death protein ligand 1 (PD-1/PD-L1) axis is one of the most widely recognized targets for cancer immunotherapy. Importantly, PD-L1 conformational changes can hinder target binding when living cells are used. Antibody affinity, equilibrium binding, association and dissociation rates, and other affinity-related constants are fundamental to ensure target saturation. Here, PD-L1 changes in conformation and their potential impact on PD-L1 function and mutation are explored. Specifically, we present detailed flow cytometry procedures to analyze PD-L1 reactivity in myeloid-derived suppressor cells (MDSCs). This approach can also be used to study the contribution of protein conformational changes in living cells. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Sample preparation for PD-L1
    MeSH term(s) Humans ; B7-H1 Antigen/metabolism ; Flow Cytometry ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; Myeloid-Derived Suppressor Cells/metabolism ; Immunotherapy/methods
    Chemical Substances B7-H1 Antigen
    Language English
    Publishing date 2023-12-15
    Publishing country United States
    Document type Journal Article
    ISSN 2691-1299
    ISSN (online) 2691-1299
    DOI 10.1002/cpz1.944
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  6. Article: COVID-19 contact tracing apps: a stress test for privacy, the GDPR, and data protection regimes.

    Bradford, Laura / Aboy, Mateo / Liddell, Kathleen

    Journal of law and the biosciences

    2020  Volume 7, Issue 1, Page(s) lsaa034

    Abstract: Digital surveillance has played a key role in containing the COVID-19 outbreak in China, Singapore, Israel, and South Korea. Google and Apple recently announced the intention to build interfaces to allow Bluetooth contact tracking using Android and ... ...

    Abstract Digital surveillance has played a key role in containing the COVID-19 outbreak in China, Singapore, Israel, and South Korea. Google and Apple recently announced the intention to build interfaces to allow Bluetooth contact tracking using Android and iPhone devices. In this article, we look at the compatibility of the proposed Apple/Google Bluetooth exposure notification system with Western privacy and data protection regimes and principles, including the General Data Protection Regulation (GDPR). Somewhat counter-intuitively, the GDPR's expansive scope is not a hindrance, but rather an advantage in conditions of uncertainty such as a pandemic. Its principle-based approach offers a functional blueprint for system design that is compatible with fundamental rights. By contrast, narrower, sector-specific rules such as the US Health Insurance Portability and Accountability Act (HIPAA), and even the new California Consumer Privacy Act (CCPA), leave gaps that may prove difficult to bridge in the middle of an emergency.
    Keywords covid19
    Language English
    Publishing date 2020-05-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2756090-9
    ISSN 2053-9711
    ISSN 2053-9711
    DOI 10.1093/jlb/lsaa034
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  7. Article ; Online: Inflammatory Bowel Disease Presenting With Retiform Purpura.

    Quinn, Laura A / Plost, Grant R / Siegele, Bradford / Feldman, Amy G / de Zoeten, Edwin

    JPGN reports

    2022  Volume 3, Issue 3, Page(s) e232

    Language English
    Publishing date 2022-08-16
    Publishing country United States
    Document type Journal Article
    ISSN 2691-171X
    ISSN (online) 2691-171X
    DOI 10.1097/PG9.0000000000000232
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  8. Article: International transfers of health data between the EU and USA: a sector-specific approach for the USA to ensure an 'adequate' level of protection.

    Bradford, Laura / Aboy, Mateo / Liddell, Kathleen

    Journal of law and the biosciences

    2020  Volume 7, Issue 1, Page(s) lsaa055

    Abstract: International health research increasingly depends on collaboration and combination using medical data to advance treatment and drug discovery. The European Union (EU), through its General Data Protection Regulation, has tightened the rules for sharing ... ...

    Abstract International health research increasingly depends on collaboration and combination using medical data to advance treatment and drug discovery. The European Union (EU), through its General Data Protection Regulation, has tightened the rules for sharing data across borders to protect individual privacy. These new rules threaten cooperation between the EU and the USA, the two largest public funders of biomedical research. This article analyzes the primary pathway for sharing research data with the USA, the US-EU Privacy Shield, and argues that the Shield is ill-suited to support complex health studies. Its legitimacy is in question under both EU and US law, and its terms are too restrictive for the variety of exchanges underlying research, treatment, and care. As an alternative, we propose that the USA seek an additional sector-based adequacy determination based on the existing US health privacy law, the Health Insurance Portability and Accountability Act. A sector-specific approach to adequacy for health would avoid many of the most contentious issues that divide the USA and EU on data protection. It could also serve as a model for other third-party jurisdictions and facilitate international harmonization of health research practices.
    Language English
    Publishing date 2020-10-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2756090-9
    ISSN 2053-9711
    ISSN 2053-9711
    DOI 10.1093/jlb/lsaa055
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  9. Article ; Online: Flow Cytometric Quantification of Cytotoxic Activity in Whole Blood Samples.

    Rico, Laura G / Ward, Michael D / Bradford, Jolene A / Petriz, Jordi

    Current protocols

    2021  Volume 1, Issue 8, Page(s) e215

    Abstract: Current methods for the determination of cell-mediated cytotoxic activity in blood samples usually isolate peripheral blood mononuclear cells by density gradient centrifugation or alternatively use erythrocyte lysis. Both centrifugation and red cell ... ...

    Abstract Current methods for the determination of cell-mediated cytotoxic activity in blood samples usually isolate peripheral blood mononuclear cells by density gradient centrifugation or alternatively use erythrocyte lysis. Both centrifugation and red cell lysis can cause cellular depletion and cell dysfunction, resulting in erroneous measurements. To address limitations of current assays, we developed an improved strategy to determine cellular cytotoxicity using flow cytometry. Viable nucleic acid stains are used to identify live nucleated cells and discriminate them from non-nucleated erythrocytes, platelets, and debris while avoiding lysing and washing steps to maintain cell functionality. To detect target cells, we have used two different labeling approaches. In the first approach, EGFP-labeled K562 human chronic myelogenous leukemia cells provide a "ready-to-use" target without the need of additional for labeling or staining. For the second approach, we perform parallel cytotoxicity assays in the presence of wild-type K562 cells previously loaded with a fluorescent dye that has spectral properties similar to those of EGFP. Given the importance of cytotoxic assays and the deleterious effects of current sample preparation methods, the aim of this study was to adapt this "untouched cells" flow cytometry method to study cytotoxic activity using unlysed whole blood samples and fluorescent target cells. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Sample preparation for cell-mediated cytotoxic activity determination in unlysed whole blood Basic Protocol 2: Protocol preparation, sample acquisition, and gating strategy for flow cytometric identification of cell-mediated cytotoxic activity using unlysed whole blood samples Support Protocol 1: Optimization of the performance of target cell labeling approaches Support Protocol 2: Assessment of the linearity and reproducibility of cytotoxicity assays.
    MeSH term(s) Flow Cytometry ; Fluorescent Dyes ; Humans ; Killer Cells, Natural ; Leukocytes, Mononuclear ; Reproducibility of Results
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2021-08-09
    Publishing country United States
    Document type Journal Article
    ISSN 2691-1299
    ISSN (online) 2691-1299
    DOI 10.1002/cpz1.215
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  10. Article ; Online: A Novel Flow Cytometric Method to Study Cytotoxic Activity in Whole Blood Samples.

    Rico, Laura G / Ward, Michael D / Bradford, Jolene A / Petriz, Jordi

    Cytometry. Part A : the journal of the International Society for Analytical Cytology

    2020  Volume 99, Issue 5, Page(s) 503–510

    Abstract: For several decades, cell-mediated cytotoxicity has been measured using ... ...

    Abstract For several decades, cell-mediated cytotoxicity has been measured using the
    MeSH term(s) Cytotoxicity Tests, Immunologic ; Cytotoxicity, Immunologic ; Flow Cytometry ; Fluorescent Dyes ; Humans ; Killer Cells, Natural
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2020-09-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2099868-5
    ISSN 1552-4930 ; 0196-4763 ; 1552-4922
    ISSN (online) 1552-4930
    ISSN 0196-4763 ; 1552-4922
    DOI 10.1002/cyto.a.24226
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    Zs.A 1688: Show issues Location:
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