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Article ; Online: ATF3 affects myocardial fibrosis remodeling after myocardial infarction by regulating autophagy and its mechanism of action.

Huang, Yiwei / Dai, Haiyue

2023 Volume 885, Page(s) 147705

Abstract: Background & objective: Myocardial fibrosis remodeling is a key event in the development of heart anomalousness and dysfunction after myocardial infarction (MI). The purpose of this study was to explore the effect of activating transcription factor 3 ( ... ...

Abstract	Background & objective: Myocardial fibrosis remodeling is a key event in the development of heart anomalousness and dysfunction after myocardial infarction (MI). The purpose of this study was to explore the effect of activating transcription factor 3 (ATF3) on myocardial fibrosis remodeling after MI and its underlying mechanism, so as to provide a theoretical basis for the clinical development of new strategies for MI treatment. Methods: MI mouse formers were structured by hypodesmus of the left anterior descending (LAD) arteria coronaria of mice, and primary cardiac fibroblasts (CFs) were separated and cultivated to investigate the effect of ATF3 on myocardial fibrosis after MI and its mechanism. Results: Increased collagen content and autophagic flux were found in the left ventricle (LV) tissues of MI mice as shown by Sirius red staining and Western blotting (WB) analysis. Meanwhile, immunofluorescence staining and WB analysis showed that ATF3 was raised in response to MI damage. After remedy with angiotensin II (AngII), the activity and differentiation of CFs were significantly raised, the expression of collagens was increased, and the level of autophagy was notably increased. Furthermore, AngII stimulation remarkably raised the expression of ATF3. Interestingly, knockdown of ATF3 in AngII-CFs reversed the above changes. In addition, after intervention with 3-methyladenine (3-MA), an autophagy restrainer, the activity and differentiation of AngII-CFs, as well as the relative collagen levels and autophagic flux were reduced. However, up-regulation of ATF3 protein expression partially reversed the effect of 3-MA on AngII-CFs. Conclusion: ATF3 can regulate the proliferation of CFs and collagen production by affecting autophagy, thus affecting myocardial fibrosis remodeling after MI.
MeSH term(s)	Animals ; Mice ; Activating Transcription Factor 3/genetics ; Autophagy ; Cardiomyopathies ; Collagen/metabolism ; Fibroblasts/metabolism ; Fibrosis ; Myocardial Infarction/genetics ; Myocardial Infarction/metabolism ; Myocardium/metabolism
Chemical Substances	Activating Transcription Factor 3 ; Collagen (9007-34-5) ; Atf3 protein, mouse
Language	English
Publishing date	2023-08-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2023.147705
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Zs.A 1357: Show issues

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Article ; Online: Enhancing Skin Injury Repair: Combined Application of PF-127 Hydrogel and hADSC-Exos Containing miR-148a-3p.

Zhang, Qiqi / Su, Peng / Zhao, Feng / Ren, Haiyue / He, Cai / Wu, Quan / Wang, Zitong / Ma, Jiajie / Huang, Xing / Wang, Zhe

ACS biomaterials science & engineering

2024 Volume 10, Issue 4, Page(s) 2235–2250

Abstract: The use of exosomes to relieve skin injuries has received considerable attention. The PluronicF-127 hydrogel (PF-127 hydrogel) is a novel biomaterial that can be used to carry biomolecules. This study sought to investigate the impact of exosomes ... ...

Abstract	The use of exosomes to relieve skin injuries has received considerable attention. The PluronicF-127 hydrogel (PF-127 hydrogel) is a novel biomaterial that can be used to carry biomolecules. This study sought to investigate the impact of exosomes originating from human mesenchymal stem cells (MSCs) developed from adipose tissue (hADSC-Exos) combined with a PF-127 hydrogel on tissue repair and explore the underlying mechanism using
MeSH term(s)	Humans ; Hydrogels/pharmacology ; Phosphatidylinositol 3-Kinases/genetics ; Mesenchymal Stem Cells ; MicroRNAs/genetics ; Wound Healing/genetics
Chemical Substances	Hydrogels ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; MicroRNAs
Language	English
Publishing date	2024-03-06
Publishing country	United States
Document type	Journal Article
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.3c01567
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Article ; Online: Predictors of participation in atrial fibrillation screening among community residents in China.

Gu, Haiyue / Huang, Jun / Qiao, Shuqian / Chen, Xinyi / Cao, Xi

Heart & lung : the journal of critical care

2023 Volume 61, Page(s) 59–64

Abstract: Background: Atrial fibrillation (AF) is associated with increased stroke risk, but many cases of AF remain undiagnosed. Screening is suggested for early detection of AF. However, nonparticipation in screening is frequently reported, and the underlying ... ...

Abstract	Background: Atrial fibrillation (AF) is associated with increased stroke risk, but many cases of AF remain undiagnosed. Screening is suggested for early detection of AF. However, nonparticipation in screening is frequently reported, and the underlying causes of why patients choose to participate or not are poorly understood. Objectives: To explore factors associated with participation in AF screening in a high-risk population in China. Methods: A cross-sectional study was conducted among community residents who were at risk for AF in Guangzhou, China, from February to September 2022. Data on AF knowledge, attitude, subjective norms, perceived behavioral control, and willingness to participate in AF screening were collected. Sociodemographic and clinical data were also collected. Multiple linear stepwise regression analysis was performed to explore predictors for participation in AF screening. Results: In total, 420 participants were included, with a mean age of 74.4 (±7.43) years old. The results showed that 77.4% of participants reported that they were likely to participate in AF screening. The results of multiple linear regression showed that perceived behavioral control (β =0.591, 95% CI: 0.071, 0.094), attitude (β =0.085, 95% CI: 0.001, 0.025), and having an AF diagnosis (β =0.098, 95% CI: 0.068, 0.494) were positively associated with willingness to participate in AF screening. Conclusions: Chinese patients are more likely to participate in AF screening when they perceive great control over their behavior, hold a positive attitude, and have an AF diagnosis. The findings provide clues for future interventions aimed at improving the AF screening participation rate.
MeSH term(s)	Humans ; Aged ; Atrial Fibrillation/diagnosis ; Atrial Fibrillation/epidemiology ; Cross-Sectional Studies ; Mass Screening/methods ; Electrocardiography ; Prevalence ; Risk Factors ; China/epidemiology
Language	English
Publishing date	2023-05-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	193129-5
ISSN	1527-3288 ; 0147-9563
ISSN (online)	1527-3288
ISSN	0147-9563
DOI	10.1016/j.hrtlng.2023.04.015
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Zs.A 1035: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Metabolic reprogramming in skin wound healing.

Wang, Zitong / Zhao, Feng / Xu, Chengcheng / Zhang, Qiqi / Ren, Haiyue / Huang, Xing / He, Cai / Ma, Jiajie / Wang, Zhe

Burns & trauma

2024 Volume 12, Page(s) tkad047

Abstract: Metabolic reprogramming refers to the ability of a cell to alter its metabolism in response to different stimuli and forms of pressure. It helps cells resist external stress and provides them with new functions. Skin wound healing involves the metabolic ... ...

Abstract	Metabolic reprogramming refers to the ability of a cell to alter its metabolism in response to different stimuli and forms of pressure. It helps cells resist external stress and provides them with new functions. Skin wound healing involves the metabolic reprogramming of nutrients, such as glucose, lipids, and amino acids, which play vital roles in the proliferation, differentiation, and migration of multiple cell types. During the glucose metabolic process in wounds, glucose transporters and key enzymes cause elevated metabolite levels. Glucose-mediated oxidative stress drives the proinflammatory response and promotes wound healing. Reprogramming lipid metabolism increases the number of fibroblasts and decreases the number of macrophages. It enhances local neovascularization and improves fibrin stability to promote extracellular matrix remodelling, accelerates wound healing, and reduces scar formation. Reprogramming amino acid metabolism affects wound re-epithelialization, collagen deposition, and angiogenesis. However, comprehensive reviews on the role of metabolic reprogramming in skin wound healing are lacking. Therefore, we have systematically reviewed the metabolic reprogramming of glucose, lipids, and amino acids during skin wound healing. Notably, we identified their targets with potential therapeutic value and elucidated their mechanisms of action.
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2775996-9
ISSN	2321-3876 ; 2321-3868
ISSN (online)	2321-3876
ISSN	2321-3868
DOI	10.1093/burnst/tkad047
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Article ; Online: Co-culture of RhoA-overexpressed Microtia Chondrocytes and Adipose-Derived Stem Cells in the Construction of Tissue-engineered Ear-shaped Cartilage.

Wu, Yi / Wang, Jian / Li, Xiu / Wang, Kang / Huang, Zonglin / Wang, Qian / Fu, Xin / Jiang, Haiyue / Pan, Bo / Xiao, Ran

Stem cells (Dayton, Ohio)

2024

Abstract: Microtia is a congenital auricle dysplasia with a high incidence and tissue engineering technology provides a promising strategy to reconstruct auricles. We previously described that the engineered cartilage constructed from microtia chondrocytes ... ...

Abstract	Microtia is a congenital auricle dysplasia with a high incidence and tissue engineering technology provides a promising strategy to reconstruct auricles. We previously described that the engineered cartilage constructed from microtia chondrocytes exhibited inferior levels of biochemical and biomechanical properties, which was proposed to be resulted from the decreased migration ability of microtia chondrocytes. In the current study, we found that Rho GTPase members were deficient in microtia chondrocytes. By overexpressing RhoA, Rac1 and CDC42, respectively, we further demonstrated that RhoA took great responsibility for the decreased migration ability of microtia chondrocytes. Moreover, we constructed PGA/PLA scaffold-based cartilages to verify the chondrogenic ability of RhoA overexpressed microtia chondrocytes, and the results showed that overexpressing RhoA was of limited help to improve the quality of microtia chondrocyte engineered cartilage. However, co-culture of ADSCs significantly improved the biochemical and biomechanical property of engineered cartilage. Especially, co-culture of RhoA overexpressed microtia chondrocytes and ADSCs produced an excellent effect on the wet weight, cartilage-specific extracellular matrix and biomechanical property of engineered cartilage. Furthermore, we presented that co-culture of RhoA overexpressed microtia chondrocytes and ADSCs combined with human ear-shaped PGA/PLA scaffold and titanium alloy stent fabricated by CAD/CAM and 3D printing technology effectively constructed and maintained auricle structure in vivo. Collectively, our results provide evidence for the essential role of RhoA in microtia chondrocytes and a developed strategy for the construction of patient-specific tissue-engineered auricular cartilage.
Language	English
Publishing date	2024-04-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1143556-2
ISSN	1549-4918 ; 1066-5099
ISSN (online)	1549-4918
ISSN	1066-5099
DOI	10.1093/stmcls/sxae026
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Zs.A 1894: Show issues

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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Adipose mesenchymal stem cell-derived exosomes promote skin wound healing in diabetic mice by regulating epidermal autophagy.

Ren, Haiyue / Su, Peng / Zhao, Feng / Zhang, Qiqi / Huang, Xing / He, Cai / Wu, Quan / Wang, Zitong / Ma, Jiajie / Wang, Zhe

Burns & trauma

2024 Volume 12, Page(s) tkae001

Abstract: Background: Adipose mesenchymal stem cell-derived exosomes (ADSC-Exos) have great potential in the field of tissue repair and regenerative medicine, particularly in cases of refractory diabetic wounds. Interestingly, autophagy plays a role in wound ... ...

Abstract	Background: Adipose mesenchymal stem cell-derived exosomes (ADSC-Exos) have great potential in the field of tissue repair and regenerative medicine, particularly in cases of refractory diabetic wounds. Interestingly, autophagy plays a role in wound healing, and recent research has demonstrated that exosomes are closely associated with intracellular autophagy in biogenesis and molecular signaling mechanisms. Therefore, this study aimed to investigate whether ADSC-Exos promote the repair of diabetic wounds by regulating autophagy to provide a new method and theoretical basis for the treatment of diabetic wounds. Methods: Western blot analysis and autophagy double-labelled adenovirus were used to monitor changes in autophagy flow in human immortalized keratinocyte cell line (HaCaT) cells. ADSC-Exos were generated from ADSC supernatants via ultracentrifugation. The effectiveness of ADSC-Exos on HaCaT cells was assessed using a live-cell imaging system, cell counting kit-8 and cell scratch assays. The cells were treated with the autophagy inhibitor bafilomycin A1 to evaluate the effects of autophagy on cell function. The recovery of diabetic wounds after ADSC-Exo treatment was determined by calculating the healing rates and performing histological analysis. High-throughput transcriptome sequencing was used to analyze changes in mRNA expression after the treatment of HaCaT cells with ADSC-Exos. Results: ADSC-Exos activated autophagy in HaCaT cells, which was inhibited by high glucose levels, and potentiated their cellular functions. Moreover, ADSC-Exos in combination with the autophagy inhibitor bafilomycin A1 showed that autophagy defects further impaired the biological function of epidermal cells under high-glucose conditions and partially weakened the healing effect of ADSC-Exos. Using a diabetes wound model, we found that ADSC-Exos promoted skin wound healing in diabetic mice, as evidenced by increased epidermal autophagy and rapid re-epithelialization. Finally, sequencing results showed that increased expression of autophagy-related genes nicotinamide phosphoribosyltransferase ( Conclusions: This study elucidated the molecular mechanism through which ADCS-Exos regulate autophagy in skin epithelial cells, thereby providing a new theoretical basis for the treatment and repair of skin epithelial damage by ADSC-Exos.
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2775996-9
ISSN	2321-3876 ; 2321-3868
ISSN (online)	2321-3876
ISSN	2321-3868
DOI	10.1093/burnst/tkae001
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Ethanol Extract of

Wu, Hongtan / Lin, Tingting / Chen, Yupei / Chen, Fangfang / Zhang, Shudi / Pang, Haiyue / Huang, Lisen / Yu, Chihli / Wang, Gueyhorng / Wu, Chun

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 6

Abstract: The fruit ... ...

Abstract	The fruit of
MeSH term(s)	Animals ; Mice ; NF-kappa B/metabolism ; AMP-Activated Protein Kinases/metabolism ; Rosa/metabolism ; Lipopolysaccharides/pharmacology ; Fruit/metabolism ; Macrophages ; Signal Transduction ; Anti-Inflammatory Agents/therapeutic use ; RAW 264.7 Cells ; Nitric Oxide/metabolism ; Mammals/metabolism
Chemical Substances	NF-kappa B ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Lipopolysaccharides ; Anti-Inflammatory Agents ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2023-03-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28062813
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Article ; Online: Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles

Jianguo Chen / Ruiquan Liu / Tianyu Huang / Hengyun Sun / Haiyue Jiang

Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-

a survey of minimal information implementation, mass production and functional modification

2022 Volume 16

Abstract: Abstract Objectives To investigate current situation of minimal information implementation highlighted by minimal information for studies of extracellular vesicles 2018 (MISEV2018) guidelines, and explore technological advances towards mass production ... ...

Abstract	Abstract Objectives To investigate current situation of minimal information implementation highlighted by minimal information for studies of extracellular vesicles 2018 (MISEV2018) guidelines, and explore technological advances towards mass production and functional modification in aesthetic, plastic and reconstructive surgery. Methods Original articles on extracellular vesicles (EVs) of adipose stem cells (ASCs) were identified. Statistics upon minimal information for EVs research, such as species, cell types, culture conditions, conditioned media harvesting parameters, EVs isolation/storage/identification/quantification, functional uptake and working concentration, were analyzed. Results The items of cell culture conditions such as passage number, seeding density, conditioned media harvesting time, functional uptake and working concentration were poorly documented, with a reporting percentage of 47.13%, 54.02%, 29.89%, 62.07% and 36.21%, respectively. However, there were some studies not reporting information of ASCs origin, culture medium, serum, EVs isolation methods, quantification and identification of EVs, accounting for 3.45%, 10.34%, 6.90%, 3.45%, 18.39% and 4.02%, respectively. Serum deprivation and trophic factors stimuli were attempted for EVs mass production. Several technological advances towards functional modification included hypoxia pre-condition, engineering EVs and controlled release. Presently, ASCs EVs have been applied in multiple fields, including diabetic/non-diabetic wound healing, angiogenesis, inflammation modulation, fat grafting, hair regeneration, antiaging, and healing and regeneration of cartilage/bone/peripheral nerve/tendon. Conclusion Our results highlight normative reporting of ASCs EVs in functional studies to increase reliability and reproducibility of scientific publications. The advances towards mass production and functional modification of ASCs EVs are also recommended to enhance therapeutic effects.
Keywords	Adipose stem cells ; Extracellular vesicles ; Functional modification ; Mass production ; Minimal information ; MISEV2018 guidelines ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
Subject code	571
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: On-Mask Chemical Modulation of Respiratory Droplets.

Huang, Haiyue / Park, Hun / Liu, Yihan / Huang, Jiaxing

Matter

2020 Volume 3, Issue 5, Page(s) 1791–1810

Abstract: Transmission of infectious respiratory diseases starts from pathogen-laden respiratory droplets released during coughing, sneezing, or speaking. Here we report an on-mask chemical modulation strategy, whereby droplets escaping a masking layer are ... ...

Abstract	Transmission of infectious respiratory diseases starts from pathogen-laden respiratory droplets released during coughing, sneezing, or speaking. Here we report an on-mask chemical modulation strategy, whereby droplets escaping a masking layer are chemically contaminated with antipathogen molecules (e.g., mineral acids or copper salts) preloaded on polyaniline-coated fabrics. A colorimetric method based on the color change of polyaniline and a fluorometric method utilizing fluorescence quenching microscopy are developed for visualizing the degree of modification of the escaped droplets by H
Keywords	covid19
Language	English
Publishing date	2020-10-29
Publishing country	United States
Document type	Journal Article
ISSN	2590-2385
ISSN (online)	2590-2385
DOI	10.1016/j.matt.2020.10.012
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Autophagy and skin wound healing.

Ren, Haiyue / Zhao, Feng / Zhang, Qiqi / Huang, Xing / Wang, Zhe

Burns & trauma

2022 Volume 10, Page(s) tkac003

Abstract: Autophagy is a lysosome-dependent, self-renewal mechanism that can degrade and recycle cellular components in eukaryotic cells to maintain the stability of the intracellular environment and the cells ability to cope with unfavorable environments. ... ...

Abstract	Autophagy is a lysosome-dependent, self-renewal mechanism that can degrade and recycle cellular components in eukaryotic cells to maintain the stability of the intracellular environment and the cells ability to cope with unfavorable environments. Numerous studies suggest that autophagy participates in regulating various cellular functions and is closely associated with the onset and progression of various diseases. Wound healing is a complex, multistep biological process that involves multiple cell types. Refractory wounds, which include diabetic skin ulcers, can seriously endanger human health. Previous studies have confirmed that autophagy plays an essential role in various phases of wound healing. Specifically, in the inflammatory phase, autophagy has an anti-infection effect and it negatively regulates the inflammatory response, which prevents excessive inflammation from causing tissue damage. In the proliferative phase, local hypoxia in the wound can induce autophagy, which plays a role in anti-apoptosis and anti-oxidative stress and promotes cell survival. Autophagy of vascular endothelial cells promotes wound angiogenesis and that of keratinocytes promotes their differentiation, proliferation and migration, which is conducive to the completion of wound re-epithelialisation. In the remodeling phase, autophagy of fibroblasts affects the formation of hypertrophic scars. Additionally, a refractory diabetic wound may be associated with increased levels of autophagy, and the regulation of mesenchymal stem cell autophagy may improve its application to wound healing. Therefore, understanding the relationship between autophagy and skin wound healing and exploring the molecular mechanism of autophagy regulation may provide novel strategies for the clinical treatment of wound healing.
Language	English
Publishing date	2022-02-16
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2775996-9
ISSN	2321-3876 ; 2321-3868
ISSN (online)	2321-3876
ISSN	2321-3868
DOI	10.1093/burnst/tkac003
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